FastQCFastQC Report
Thu 26 May 2016
SRR1364315_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364315_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1633593
Sequences flagged as poor quality0
Sequence length25
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT121260.7422901542795544No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA70780.4332780564069508No Hit
TATCAACGCAGAGTACTTTTTTTTT70370.43076825133310437No Hit
GTACTTTTTTTTTTTTTTTTTTTTT58980.3610446420864928No Hit
GGTATCAACGCAGAGTACTTTTTTT52640.32223448557872125No Hit
NNNNNNNNNNNNNNNNNNNNNNNNN48190.2949939183138028No Hit
GAAAAAAAAAAAAAAAAAAAAAAAA31910.19533629245473016No Hit
GAGTACTTTTTTTTTTTTTTTTTTT28660.17544149613765486No Hit
ACGCAGAGTACTTTTTTTTTTTTTT26400.1616069608525502No Hit
GAACAAAAAAAAAAAAAAAAAAAAA25280.15475090796789653No Hit
GTGGTATCAACGCAGAGTACATGGG20220.12377624047115775No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAACGC2300.015.6847933
TATAACG2300.015.2720362
TATCACG1450.014.4038212
TAACGCA2650.013.9860644
CGCATAT1101.8553692E-1013.8179598
GGTATCA27650.012.2573381
CTTACTC802.8869283E-511.8667843
ATCACGC1701.8189894E-1211.7271753
TTGACCG1751.8189894E-1211.40928318
TTACTCT1751.8189894E-1211.4040214
GTCTTAA1850.011.2894811
ACGCATA5400.011.2590767
GTCCATA855.3606418E-511.1687381
GTATCAC2300.011.1444591
CGTGACA600.00584036311.09235914
CGTCAAA600.00584036311.09235914
ACAACCG600.005860451611.0872445
GGGCGAT600.00587656511.0831557
GTCTTAG2150.011.0388691
GTTATCA4500.010.9701831
Produced by FastQC (version 0.11.5)