##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1364315_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1633593
Sequences flagged as poor quality	0
Sequence length	25
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	26.147957906283878	30.0	26.0	31.0	7.0	34.0
2	26.0249541960574	31.0	26.0	31.0	7.0	34.0
3	25.51007197019086	30.0	25.0	31.0	7.0	34.0
4	29.162079538783527	35.0	28.0	37.0	7.0	37.0
5	29.860553393654357	35.0	30.0	37.0	7.0	37.0
6	29.93225179099078	35.0	30.0	37.0	2.0	37.0
7	29.525960872751046	35.0	30.0	37.0	2.0	37.0
8	28.99722758361477	35.0	28.0	37.0	2.0	37.0
9	30.00005631757727	35.0	28.0	39.0	2.0	39.0
10	29.405232515075664	35.0	27.0	39.0	2.0	39.0
11	29.992973770088387	35.0	28.0	39.0	2.0	39.0
12	29.554227399358346	35.0	27.0	39.0	2.0	39.0
13	29.111129883636867	35.0	26.0	39.0	2.0	39.0
14	29.435657474046472	36.0	25.0	40.0	2.0	41.0
15	30.149881886124636	36.0	27.0	40.0	2.0	41.0
16	30.488386030057672	37.0	27.0	40.0	2.0	41.0
17	30.684429965113708	37.0	29.0	40.0	2.0	41.0
18	30.53351722246606	37.0	28.0	40.0	2.0	41.0
19	30.543320153795957	37.0	27.0	40.0	2.0	41.0
20	30.44047017831247	37.0	27.0	40.0	2.0	41.0
21	30.348460112157678	37.0	27.0	40.0	2.0	41.0
22	30.42337412072652	37.0	28.0	40.0	2.0	41.0
23	30.439136308737854	37.0	29.0	40.0	2.0	41.0
24	30.32871345555472	37.0	28.0	40.0	2.0	41.0
25	30.154326689695658	37.0	28.0	40.0	2.0	41.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1101	1	0.9939982878733886
1101	2	1.0509027191982199
1101	3	1.8464147235051804
1101	4	1.2061983740370792
1101	5	0.7918654909713254
1101	6	0.672091502007305
1101	7	0.9469271869026308
1101	8	1.2516710738439407
1101	9	1.2342341458727155
1101	10	1.543820351538308
1101	11	1.114824881941196
1101	12	1.3156778138227772
1101	13	1.3650221079776053
1101	14	1.8818079336076288
1101	15	1.3029925255361015
1101	16	0.8479426953739875
1101	17	0.1220208673977119
1101	18	-5.415729363296904
1101	19	-6.542371380736629
1101	20	-7.800779466740469
1101	21	-9.64857046721729
1101	22	-9.457762946826659
1101	23	-10.98761036754955
1101	24	-10.618835927178914
1101	25	-11.25237080540089
1102	1	1.842821286096477
1102	2	1.9212702065672609
1102	3	2.741310390623898
1102	4	2.1572308601276475
1102	5	1.459380695057252
1102	6	1.5363681306003727
1102	7	1.8683242473920743
1102	8	2.1544672015356063
1102	9	2.168018935637189
1102	10	-0.971521129480454
1102	11	1.109318559177627
1102	12	2.0338529674819554
1102	13	2.4353630549518357
1102	14	2.933955278111668
1102	15	2.4154757354997543
1102	16	2.0623025054483364
1102	17	1.8623468934914484
1102	18	2.0143559543624967
1102	19	1.8003696594526488
1102	20	2.0855344284508632
1102	21	2.106434824678505
1102	22	2.0638498352864083
1102	23	2.0176083803264078
1102	24	2.2209798436415404
1102	25	2.1888854291644186
1103	1	-0.7535852508746359
1103	2	-0.3962189977795596
1103	3	-2.8809318554628334
1103	4	-0.6096726461823323
1103	5	-3.0755026823449967
1103	6	-7.190020532609715
1103	7	-7.326057581443273
1103	8	-2.539990947766924
1103	9	-6.853475862261291
1103	10	-4.205704820535203
1103	11	-8.787161343919998
1103	12	-2.5933565100428986
1103	13	-0.7565410668457808
1103	14	-0.19183168438297926
1103	15	-0.41117241729874365
1103	16	-0.845034599593248
1103	17	-0.844096031108851
1103	18	-0.3850482536033937
1103	19	-5.3808328556760365
1103	20	-1.5520191805224535
1103	21	-6.702682270613753
1103	22	-2.777401623419294
1103	23	-1.8059081713671645
1103	24	-7.68965135124154
1103	25	-4.3238561497337145
1104	1	1.5718178090138935
1104	2	1.6294608735405305
1104	3	2.4592282462840735
1104	4	1.8146156865898995
1104	5	1.5500163390891863
1104	6	1.3504537875189655
1104	7	1.4585719027148834
1104	8	1.8437669544513078
1104	9	2.026242089334115
1104	10	2.299756594195653
1104	11	1.872528926532251
1104	12	2.1609359374938535
1104	13	2.366808351476145
1104	14	2.8151821187816495
1104	15	1.9266684535350578
1104	16	1.01850484603575
1104	17	0.8864991134690108
1104	18	0.8601917119822104
1104	19	1.268458282617459
1104	20	-9.1817090769052
1104	21	-3.3084504223363425
1104	22	-0.8950651853090932
1104	23	0.2024522820050727
1104	24	0.6658643035671226
1104	25	1.052513879817777
1105	1	1.4072899911225747
1105	2	1.255347043071069
1105	3	2.2332247936449683
1105	4	1.5468233843130683
1105	5	1.1877902806012912
1105	6	1.1325823818620933
1105	7	1.4228866353862237
1105	8	1.6369874263119861
1105	9	1.5897969905293685
1105	10	2.1482699367172096
1105	11	1.6928140436507242
1105	12	1.9088511048025971
1105	13	2.0368045727021915
1105	14	2.767830945244981
1105	15	2.101756611860978
1105	16	1.5251183276350027
1105	17	0.9730606330065257
1105	18	1.4938315807129356
1105	19	1.4657806841615795
1105	20	1.5115139036850707
1105	21	1.5690749667911348
1105	22	1.3288820498356984
1105	23	1.3164095991069225
1105	24	1.3339405626749858
1105	25	1.3246453435203804
1106	1	1.1056833761463736
1106	2	1.412982391630841
1106	3	1.8805864028277206
1106	4	1.446294278720817
1106	5	0.7998318785878986
1106	6	0.5116747763778946
1106	7	1.1770089142005489
1106	8	1.1396045651864526
1106	9	1.4510719635557479
1106	10	-1.7131390072805317
1106	11	0.16820349646702581
1106	12	0.8240103189352581
1106	13	1.60703367103768
1106	14	0.9825575447826225
1106	15	1.537385553737515
1106	16	1.0472690720909554
1106	17	-5.462321578997749
1106	18	-1.5377452177476734
1106	19	-0.04647518763457725
1106	20	0.6699860923480969
1106	21	1.0742771074558704
1106	22	1.0753164682541545
1106	23	0.51591916965819
1106	24	0.952348174140031
1106	25	1.0360618233852605
1107	1	0.8330940256866732
1107	2	1.3645986026478418
1107	3	1.827020431209963
1107	4	1.3448924499435648
1107	5	0.9030752279479763
1107	6	1.0240776045827964
1107	7	1.0671462353086376
1107	8	1.1230017612784167
1107	9	1.2950773389234875
1107	10	-1.5080761485417398
1107	11	0.3585094553351915
1107	12	0.7077842729490627
1107	13	1.4276115168077084
1107	14	2.014764351160423
1107	15	1.5829822483622742
1107	16	-4.663622998490176
1107	17	-1.476822046774977
1107	18	0.30569015197167104
1107	19	0.9431108201278917
1107	20	1.3124882872064312
1107	21	1.452796551834215
1107	22	1.093715236127693
1107	23	0.8601977281779369
1107	24	1.2087270398773207
1107	25	1.2972656202083819
1108	1	-0.46681054597357985
1108	2	-3.0844399444680484
1108	3	-0.03762089667785773
1108	4	-0.9320104307067325
1108	5	-0.9506449939599975
1108	6	-0.650129796305233
1108	7	-0.23259640484246447
1108	8	0.07062866119238365
1108	9	-0.5056109317963262
1108	10	-3.7913388583110326
1108	11	-1.7081902568964473
1108	12	-5.87899371029906
1108	13	-1.6837763474763783
1108	14	-5.57094686191672
1108	15	-2.652927238104702
1108	16	-7.460339620821397
1108	17	-3.237583693482449
1108	18	-1.9120953666541602
1108	19	-0.7141345642060379
1108	20	-0.6015248453153497
1108	21	-0.17247663033007754
1108	22	-0.3720418122193685
1108	23	-0.33827220447833284
1108	24	-0.004604744187627574
1108	25	-0.3182842546964366
1109	1	-8.427789868264497
1109	2	-5.847607308066166
1109	3	-5.249152751608431
1109	4	-5.265953202368365
1109	5	-2.4796329928365495
1109	6	-1.5245145820244268
1109	7	-1.3061204631412124
1109	8	-0.7450680271677577
1109	9	-5.505805003800656
1109	10	-1.6496021067388682
1109	11	-1.1789432442190844
1109	12	-6.213058228994463
1109	13	-7.9088860102129885
1109	14	-8.057464432665608
1109	15	-3.966350963588045
1109	16	-2.310122615774322
1109	17	-1.8940949297693344
1109	18	-1.3124134759829964
1109	19	-1.0860416169755425
1109	20	-0.7165066314769319
1109	21	-0.7859780147505582
1109	22	-0.9260738389217487
1109	23	-1.1290842813019175
1109	24	-0.825768296259799
1109	25	-0.724963043831778
1110	1	-2.587991882487838
1110	2	-2.5158004114504138
1110	3	-1.634589918616161
1110	4	-2.475093601760342
1110	5	-5.2955573748850036
1110	6	-4.118937357702059
1110	7	-8.65917111699057
1110	8	-9.092613963164187
1110	9	-11.243060301719847
1110	10	-8.545968920420453
1110	11	-11.789356467263666
1110	12	-6.56134881426587
1110	13	-4.806886177915025
1110	14	-4.427200859028023
1110	15	-9.389686470084467
1110	16	-10.763546711307615
1110	17	-11.663564976821654
1110	18	-8.498815016752726
1110	19	-11.712890220043676
1110	20	-12.865784801865104
1110	21	-13.805299291525944
1110	22	-14.392258811948704
1110	23	-15.093432220670378
1110	24	-15.657854889700445
1110	25	-16.122418615122697
1111	1	-0.22217329174781852
1111	2	0.46454337997149153
1111	3	-2.36656481011644
1111	4	-2.7432257464243044
1111	5	-0.820627865844596
1111	6	0.05537431238539625
1111	7	0.4919425396880932
1111	8	0.6551747570590081
1111	9	-3.7267868728141416
1111	10	-3.5853564813327665
1111	11	-0.7893070995634233
1111	12	-5.310872394114412
1111	13	-0.7297681506658016
1111	14	0.5137094988397486
1111	15	0.5711308668033404
1111	16	0.44605849563042455
1111	17	0.4386707246758945
1111	18	0.7668937417763111
1111	19	0.8612539493687947
1111	20	1.0617822967034058
1111	21	1.143847171518619
1111	22	0.7935568429422375
1111	23	0.6957746894096317
1111	24	0.8277699350005321
1111	25	1.0130044136425056
1112	1	-0.40987249121036484
1112	2	0.09975583096847984
1112	3	-2.4500913562672935
1112	4	-0.5404688499185824
1112	5	-0.4515968516336706
1112	6	-0.22040150213729603
1112	7	0.20669962514217488
1112	8	-3.225496044602515
1112	9	-0.7981122536959795
1112	10	-3.0996529176880045
1112	11	-0.8475460571283335
1112	12	0.16900263754099853
1112	13	0.6047041213813422
1112	14	1.2212620533821834
1112	15	0.6099693767530887
1112	16	0.5146931119318587
1112	17	0.23759336913062867
1112	18	0.2792035960892534
1112	19	0.2909863731257296
1112	20	0.48782672843465846
1112	21	0.4980676086453961
1112	22	0.3362948183693284
1112	23	0.22881375317052033
1112	24	0.3928239361788357
1112	25	0.39437043154273965
1113	1	-1.73622717035866
1113	2	-1.1903266445187874
1113	3	-4.024444483185988
1113	4	-2.4095324355158034
1113	5	-2.413038830407462
1113	6	-2.0798710329865315
1113	7	-2.1975089371051553
1113	8	-8.509340896064241
1113	9	-9.689538045691897
1113	10	-4.456478608151098
1113	11	-9.422820421684339
1113	12	-8.02409419341216
1113	13	-3.662953952206035
1113	14	-2.1869839005878298
1113	15	-7.28366834242625
1113	16	-3.597587310040698
1113	17	-2.745401742940878
1113	18	-2.240252698281523
1113	19	-2.2082335251819707
1113	20	-7.694052585713752
1113	21	-3.6179775016984586
1113	22	-2.733792600862902
1113	23	-2.79499277097808
1113	24	-8.757730640373463
1113	25	-5.544547738846354
1114	1	-4.303470703012895
1114	2	-4.067492131631521
1114	3	-0.9454809448403836
1114	4	-0.8358012684161373
1114	5	-0.8029930359607604
1114	6	-1.0163195129165494
1114	7	-0.7394422110655583
1114	8	-0.4422428095238047
1114	9	-0.9145205236510137
1114	10	-3.7132821663110995
1114	11	-1.7231750783313835
1114	12	-4.54365142593096
1114	13	-1.50920739732911
1114	14	-5.78956188025699
1114	15	-2.267969647752423
1114	16	-1.491376339398677
1114	17	-1.2101553013643702
1114	18	-0.9437207449296743
1114	19	-0.7455436152426245
1114	20	-0.5941242548133054
1114	21	-0.5229790562855428
1114	22	-0.4773494961153517
1114	23	-0.8912949616284251
1114	24	-0.7674655174029894
1114	25	-0.5903211063981715
1115	1	0.44632549232429497
1115	2	0.528454248854942
1115	3	1.2036743397211112
1115	4	0.2665270822375234
1115	5	0.0016609106766445336
1115	6	-0.6039380625910198
1115	7	-2.410005235594941
1115	8	-0.1290422949187331
1115	9	0.029358258872161258
1115	10	0.6838290031662879
1115	11	-0.2583528950018277
1115	12	0.39491220735319743
1115	13	0.7485082012756621
1115	14	1.4155227494758265
1115	15	0.4803587994510572
1115	16	0.17018259649142564
1115	17	-0.09452666796157061
1115	18	0.2096251835181846
1115	19	0.38643275678072797
1115	20	0.4497659554989255
1115	21	0.68590319957638
1115	22	0.25904127535859445
1115	23	0.022871160240899968
1115	24	0.09406418080246581
1115	25	0.29890052479734663
1116	1	0.07325924486546143
1116	2	0.3502671878783232
1116	3	0.9759682188660612
1116	4	0.2788947111080624
1116	5	-0.6969491823506004
1116	6	-1.451698385961425
1116	7	-4.700191500443456
1116	8	-1.189032515919795
1116	9	-1.3494830187516236
1116	10	-0.7246661583058582
1116	11	-2.252615217052906
1116	12	-0.6345894358746165
1116	13	-6.3556571506469375
1116	14	-1.6692893001063425
1116	15	-1.5703529692748113
1116	16	-1.0877455124939814
1116	17	-0.7310488826863804
1116	18	-0.46172466893382236
1116	19	-0.16954270780262704
1116	20	-0.11188475354592242
1116	21	-0.07106130314291548
1116	22	-0.32894908372430365
1116	23	-0.5460474539999751
1116	24	-0.3710689414166808
1116	25	-0.41881691229585627
1201	1	2.221291162200192
1201	2	2.4702512702730672
1201	3	2.992099588625507
1201	4	2.5895500865002674
1201	5	2.0653803193244933
1201	6	2.029487922482634
1201	7	2.3009292535534556
1201	8	2.4049930681886877
1201	9	2.7580580373174755
1201	10	3.023686526552126
1201	11	2.5917793221698595
1201	12	2.8496553957345085
1201	13	2.958297082010965
1201	14	3.5885451084906137
1201	15	3.1382298566194393
1201	16	2.462294704217328
1201	17	2.4203495236207573
1201	18	2.3367491491417205
1201	19	2.330341352128727
1201	20	2.632921493691441
1201	21	2.625451678521646
1201	22	1.7849270101581673
1201	23	1.389501341068705
1201	24	1.8814177315139702
1201	25	1.8799515867874916
1202	1	2.458877724746234
1202	2	2.598423735535942
1202	3	3.330921889571691
1202	4	2.875599823529498
1202	5	2.1912481869230334
1202	6	2.216237206599967
1202	7	2.4858902723810097
1202	8	2.7847606545590473
1202	9	3.1104923586863364
1202	10	-0.13796234180874478
1202	11	1.935403962627003
1202	12	2.892232957697601
1202	13	3.2642579663102325
1202	14	3.81338095195094
1202	15	3.27063387709336
1202	16	3.020260059980586
1202	17	2.551527414857844
1202	18	2.6461869490276904
1202	19	2.8555852647237785
1202	20	2.8922654440825397
1202	21	3.0062243833729916
1202	22	2.8796461343149637
1202	23	2.4704982597303413
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2211	16	-0.5460225152187164
2211	17	-0.12030885498874255
2211	18	0.31801651960449817
2211	19	0.5234220705119448
2211	20	0.670673697582906
2211	21	0.8034374871446808
2211	22	0.5247188213920708
2211	23	0.4430044565548883
2211	24	0.663948196633303
2211	25	0.7065228559972674
2212	1	1.072155192779487
2212	2	1.0727619378500002
2212	3	1.8293592965818029
2212	4	1.389296499045777
2212	5	0.8508556298880272
2212	6	0.6347341561687863
2212	7	0.9645983643337637
2212	8	1.2313590209852912
2212	9	1.054700748932504
2212	10	1.783091076662803
2212	11	1.062598015919189
2212	12	1.3017475740748488
2212	13	1.5528184501987568
2212	14	-3.825343484448698
2212	15	-0.5777649912786806
2212	16	0.01985899686576431
2212	17	0.4240421807446886
2212	18	0.8078246742174429
2212	19	1.156281137533206
2212	20	1.3655382090955506
2212	21	1.4832195018121688
2212	22	1.2695860256519715
2212	23	1.1050259763414374
2212	24	1.2633223426332485
2212	25	1.2924661470107175
2213	1	0.5050300373459145
2213	2	0.5719305427384
2213	3	1.211486690903083
2213	4	0.7840721324308682
2213	5	0.24806665767171054
2213	6	-0.09687099209175543
2213	7	0.1388546992584807
2213	8	-5.205978834149548
2213	9	-1.877703097014841
2213	10	-0.03893372613253021
2213	11	-0.27463001559919675
2213	12	0.3758373078351305
2213	13	-8.07384474270209
2213	14	-2.37040311295441
2213	15	-1.0195161934056785
2213	16	-0.5181757858923284
2213	17	-0.14281156995597044
2213	18	0.05349602375667217
2213	19	0.4260802604476055
2213	20	0.4880288562361095
2213	21	0.5337421237053768
2213	22	0.17461383188563318
2213	23	0.154304861214289
2213	24	0.19761890773924407
2213	25	0.44854359963972
2214	1	-1.1041429199477157
2214	2	-0.7550997200488325
2214	3	-7.015345215919389
2214	4	-2.4684185693023366
2214	5	-1.5732873468312754
2214	6	-1.1068735125786517
2214	7	-0.8015654383728119
2214	8	-6.090824750854868
2214	9	-1.7165126646983886
2214	10	-0.06880970335071623
2214	11	-0.6545870614191074
2214	12	-0.12351747928963164
2214	13	-5.822767843494031
2214	14	-1.4686655655364262
2214	15	-0.7072049651660599
2214	16	-0.8655894274452045
2214	17	-0.7272310689933121
2214	18	-0.5004817122953966
2214	19	-0.23520257207133355
2214	20	-0.03537262026087973
2214	21	0.022286616653310887
2214	22	-0.2117353752200195
2214	23	-0.3162141790520785
2214	24	-0.03618047738903485
2214	25	-0.08660329285410029
2215	1	0.27165867073751215
2215	2	0.5152987264202373
2215	3	-2.575622456504181
2215	4	-0.42660982307289785
2215	5	-0.004040817350407622
2215	6	-0.42811762034751055
2215	7	0.17686207933228104
2215	8	0.4463244717782473
2215	9	0.8643158494904135
2215	10	1.369091025167613
2215	11	0.6794452093635783
2215	12	0.5671684185146582
2215	13	0.9910289652455155
2215	14	0.9832480814522704
2215	15	0.6342760747801606
2215	16	0.4508786151779951
2215	17	0.4664450818210746
2215	18	0.5627483597010858
2215	19	0.5178418754321754
2215	20	0.7998059542702478
2215	21	0.8654224931406524
2215	22	0.6802295393584998
2215	23	0.6442041944181476
2215	24	0.6189550355742064
2215	25	0.673636659780847
2216	1	-0.03088358997921148
2216	2	-0.3574294126258586
2216	3	0.8833416331367161
2216	4	-0.5893510387203484
2216	5	-0.6283958550184323
2216	6	-0.8632423245357295
2216	7	-1.5311596479438165
2216	8	-6.376525433191389
2216	9	-2.29081023118216
2216	10	-1.0900435367662418
2216	11	-1.6501994988482096
2216	12	-0.3687877351645277
2216	13	-0.024208981251593542
2216	14	0.767046860616535
2216	15	0.2815763124884221
2216	16	-0.5125354895512864
2216	17	-0.5277366247018733
2216	18	-0.3016501960491773
2216	19	-0.20603880455109547
2216	20	-0.03486636304116075
2216	21	-0.30618169348944946
2216	22	-0.4220278949805518
2216	23	-0.7041077592896272
2216	24	-0.750961369472197
2216	25	-0.602749221725027
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	156321.0
3	4.0
4	923.0
5	7120.0
6	12142.0
7	10906.0
8	9231.0
9	7485.0
10	6806.0
11	6284.0
12	6075.0
13	5707.0
14	5555.0
15	5398.0
16	5231.0
17	5493.0
18	6437.0
19	8815.0
20	12229.0
21	15514.0
22	19086.0
23	22017.0
24	24744.0
25	27533.0
26	29930.0
27	32379.0
28	34450.0
29	40118.0
30	45411.0
31	51001.0
32	63622.0
33	81243.0
34	102440.0
35	129203.0
36	165122.0
37	199141.0
38	272477.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	46.506058884059534	18.890632919168805	13.56391393159374	21.039394265177926
2	13.715244819860084	23.14052836290561	42.17650965437883	20.967717162855475
3	15.338707962959663	31.649690972109152	30.472073205724282	22.539527859206906
4	9.889633187955381	19.121870472300152	38.797418343182905	32.19107799656156
5	11.104542985446617	41.776576944158364	32.93983979235438	14.179040278040638
6	26.25511244304155	39.879266510274014	19.872327098097493	13.993293948586938
7	25.58230516997937	36.119165202394996	21.848366351866172	16.45016327575946
8	24.8831703235118	36.142240783675476	21.06711296043237	17.907475932380354
9	27.295802899198705	18.595197399896698	19.975642456316407	34.13335724458818
10	12.666910559857843	33.975694711309366	32.32800150025055	21.029393228582233
11	31.216175383171645	27.537383648231355	23.053065496004304	18.19337547259269
12	21.75362980168364	30.962004155363683	29.547485439952048	17.736880603000632
13	25.967198939793484	26.334500559457908	27.03833668434906	20.65996381639955
14	19.98481829861768	25.46724265419376	30.001117746622807	24.546821300565753
15	22.309321071125474	31.93928094629266	25.21158572307602	20.53981225950585
16	20.352118168636697	32.84790369442805	26.010844325767035	20.789133811168217
17	19.796566538364228	31.691602535917724	27.070258479874038	21.44157244584401
18	19.286810451712505	30.24675095784029	30.178278964113918	20.288159626333286
19	20.865133542455276	29.956779554850293	30.26880439401718	18.90928250867725
20	21.883092711829473	28.681693589818526	29.97129769489419	19.46391600345781
21	22.76579934782208	28.55261945848353	29.41592616019504	19.265655033499346
22	21.86130344668765	28.819712903968682	29.334397789207035	19.98458586013664
23	20.97834076677775	29.223476788678322	29.570002413336354	20.22818003120757
24	20.440816045797412	29.114910503571323	30.067262797771292	20.377010652859973
25	20.62106545559046	28.68923024775809	30.238485892747054	20.451218403904395
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	12993.0
1	12993.0
2	12038.5
3	11084.0
4	11084.0
5	11084.0
6	15820.5
7	20557.0
8	20557.0
9	20557.0
10	19984.5
11	19412.0
12	19412.0
13	19412.0
14	20934.0
15	22456.0
16	22456.0
17	22456.0
18	30467.0
19	38478.0
20	38478.0
21	38478.0
22	51112.5
23	63747.0
24	63747.0
25	63747.0
26	83573.0
27	103399.0
28	103399.0
29	103399.0
30	120515.0
31	137631.0
32	137631.0
33	137631.0
34	146327.5
35	155024.0
36	155024.0
37	155024.0
38	163108.0
39	171192.0
40	171192.0
41	171192.0
42	179802.0
43	188412.0
44	188412.0
45	188412.0
46	191477.5
47	194543.0
48	194543.0
49	194543.0
50	182876.0
51	171209.0
52	171209.0
53	171209.0
54	149880.5
55	128552.0
56	128552.0
57	128552.0
58	108491.5
59	88431.0
60	88431.0
61	88431.0
62	71280.5
63	54130.0
64	54130.0
65	54130.0
66	40951.5
67	27773.0
68	27773.0
69	27773.0
70	20495.0
71	13217.0
72	13217.0
73	13217.0
74	9548.5
75	5880.0
76	5880.0
77	5880.0
78	4492.5
79	3105.0
80	3105.0
81	3105.0
82	2213.5
83	1322.0
84	1322.0
85	1322.0
86	1003.0
87	684.0
88	684.0
89	684.0
90	493.5
91	303.0
92	303.0
93	303.0
94	171.5
95	40.0
96	40.0
97	40.0
98	29.5
99	19.0
100	19.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.35614746145459736
2	0.3603100649917085
3	0.371573580445068
4	0.37408338551891446
5	0.37983757276139163
6	0.3191125329258879
7	0.32847839088438796
8	0.32860082040018534
9	0.3274989547580089
10	0.440623827354794
11	0.32694802193692063
12	0.3266419481474272
13	0.3202143985680644
14	0.325478867747352
15	0.31678637212573757
16	0.32296906267350556
17	0.3151947884203715
18	0.317704593494218
19	0.3181330967995088
20	0.5073479134643696
21	0.318500385346901
22	0.31941860671538136
23	0.3147662851150807
24	0.31856160010479967
25	0.31029760778847604
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1633593.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	61.82187105582013
#Duplication Level	Percentage of deduplicated	Percentage of total
1	84.47348956582114	52.22309179573361
2	8.687546001094242	10.741606973423082
3	2.8155030711128104	5.221790034588052
4	1.2841863310906456	3.175632070893305
5	0.7057099907802304	2.1814156026409712
6	0.41518501801393126	1.5400508788779368
7	0.29765851078445255	1.2881264250668707
8	0.21289676251738393	1.0529340960441016
9	0.1592994389548731	0.8863370436899389
>10	0.8342926971235364	9.518840163215772
>50	0.07093938206295029	3.01453949692068
>100	0.039320659402335305	4.247766929921406
>500	0.0021390768222971208	0.8809188484596633
>1k	0.0013241904138029793	1.6715984582430312
>5k	4.074432042470706E-4	1.5917496413263925
>10k+	1.0186080106176765E-4	0.7636015409551703
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	12126	0.7422901542795544	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA	7078	0.4332780564069508	No Hit
TATCAACGCAGAGTACTTTTTTTTT	7037	0.43076825133310437	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	5898	0.3610446420864928	No Hit
GGTATCAACGCAGAGTACTTTTTTT	5264	0.32223448557872125	No Hit
NNNNNNNNNNNNNNNNNNNNNNNNN	4819	0.2949939183138028	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAA	3191	0.19533629245473016	No Hit
GAGTACTTTTTTTTTTTTTTTTTTT	2866	0.17544149613765486	No Hit
ACGCAGAGTACTTTTTTTTTTTTTT	2640	0.1616069608525502	No Hit
GAACAAAAAAAAAAAAAAAAAAAAA	2528	0.15475090796789653	No Hit
GTGGTATCAACGCAGAGTACATGGG	2022	0.12377624047115775	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	6.121475789869325E-5	0.0	0.0	0.0	0.0
8	1.224295157973865E-4	0.0	0.0	0.0	0.0
9	1.224295157973865E-4	0.0	0.0	1.224295157973865E-4	0.0
10	1.8364427369607975E-4	0.0	0.0	1.224295157973865E-4	0.0
11	1.8364427369607975E-4	0.0	0.0	1.224295157973865E-4	0.0
12	1.8364427369607975E-4	0.0	0.0	1.224295157973865E-4	0.0
13	3.0607378949346623E-4	0.0	0.0	1.224295157973865E-4	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATAACGC	230	0.0	15.684793	3
TATAACG	230	0.0	15.272036	2
TATCACG	145	0.0	14.403821	2
TAACGCA	265	0.0	13.986064	4
CGCATAT	110	1.8553692E-10	13.817959	8
GGTATCA	2765	0.0	12.257338	1
CTTACTC	80	2.8869283E-5	11.866784	3
ATCACGC	170	1.8189894E-12	11.727175	3
TTGACCG	175	1.8189894E-12	11.409283	18
TTACTCT	175	1.8189894E-12	11.404021	4
GTCTTAA	185	0.0	11.289481	1
ACGCATA	540	0.0	11.259076	7
GTCCATA	85	5.3606418E-5	11.168738	1
GTATCAC	230	0.0	11.144459	1
CGTGACA	60	0.005840363	11.092359	14
CGTCAAA	60	0.005840363	11.092359	14
ACAACCG	60	0.0058604516	11.087244	5
GGGCGAT	60	0.005876565	11.083155	7
GTCTTAG	215	0.0	11.038869	1
GTTATCA	450	0.0	10.970183	1
>>END_MODULE