FastQCFastQC Report
Thu 26 May 2016
SRR1364315_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364315_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1633593
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT281601.7238075824272017No Hit
TATCAACGCAGAGTACTTTTTTTTT207591.2707571592189733No Hit
GGTATCAACGCAGAGTACTTTTTTT196021.199931684330185No Hit
GTACTTTTTTTTTTTTTTTTTTTTT191481.1721401842441783No Hit
GAGTACTTTTTTTTTTTTTTTTTTT95310.5834378575324454No Hit
ACGCAGAGTACTTTTTTTTTTTTTT83370.5103474366014056No Hit
GTGGTATCAACGCAGAGTACTTTTT42180.2582038488166881No Hit
GTGGTATCAACGCAGAGTACATGGG32740.20041711736032167No Hit
ATCAACGCAGAGTACTTTTTTTTTT32170.19692787616009616No Hit
GCAGAGTACTTTTTTTTTTTTTTTT29940.1832769851486876No Hit
GTATAACGCAGAGTACTTTTTTTTT27880.17066674502155676No Hit
GGTATCAACGCAGAGTACATGGGAT21750.1331420984296578No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA17500.10712582632271318No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAACGCA4250.014.7549384
ATAACGC4250.014.5309343
TATAACG4100.014.3669182
TATCACG2100.013.5724342
GGTATCA47800.012.7209981
AACCGTG604.0907215E-412.6672187
ACCGACC550.003067560312.0914358
ATCACGC2450.012.02166753
TTAGTAC802.8634975E-511.8762443
CCTATAC802.8634975E-511.8762443
GTATAAC5350.011.7208541
TACAACG1552.5465852E-1111.6460252
GTGGTAT15750.011.64248851
TGGTATC15350.011.5741482
TCACGCA2750.011.4015454
GGTATCC1251.82863E-811.4011953
CGCTATT752.0748693E-411.39944811
TTGCGCG2100.011.306918
GCGTGCG600.00587531111.0834769
AACGCCG1658.0035534E-1110.939876
Produced by FastQC (version 0.11.5)