##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1364315_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1633593
Sequences flagged as poor quality	0
Sequence length	25
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.419368839117208	31.0	30.0	34.0	26.0	34.0
2	29.400713641647584	31.0	30.0	34.0	25.0	34.0
3	29.45640988912171	31.0	30.0	34.0	25.0	34.0
4	32.77643513408787	35.0	33.0	37.0	26.0	37.0
5	32.37034010307341	35.0	32.0	37.0	24.0	37.0
6	32.215802222463	35.0	32.0	37.0	22.0	37.0
7	31.94264850547229	35.0	32.0	37.0	18.0	37.0
8	31.804601880639794	35.0	32.0	37.0	17.0	37.0
9	32.46028845618217	37.0	32.0	39.0	11.0	39.0
10	32.612321428899364	37.0	32.0	39.0	15.0	39.0
11	32.53813097876888	37.0	32.0	39.0	11.0	39.0
12	32.50299860491567	37.0	32.0	39.0	11.0	39.0
13	32.511340952122104	37.0	32.0	39.0	11.0	39.0
14	33.39726051715452	38.0	32.0	41.0	11.0	41.0
15	33.425183628970004	38.0	32.0	41.0	11.0	41.0
16	33.39633005283446	38.0	32.0	41.0	10.0	41.0
17	33.43436584265481	38.0	32.0	41.0	10.0	41.0
18	33.4135718015442	38.0	32.0	41.0	10.0	41.0
19	33.36041963940835	39.0	32.0	41.0	10.0	41.0
20	33.27602224054584	39.0	32.0	41.0	8.0	41.0
21	33.21975853226599	38.0	32.0	41.0	2.0	41.0
22	33.14944175201534	38.0	32.0	41.0	2.0	41.0
23	32.894073370784525	38.0	32.0	41.0	2.0	41.0
24	32.81634348335234	38.0	32.0	41.0	2.0	41.0
25	32.65384584777237	38.0	32.0	41.0	2.0	41.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1101	1	0.4050789758828053
1101	2	0.5345205592900939
1101	3	-1.273725407546113
1101	4	-0.1834262273195577
1101	5	0.4794206312168754
1101	6	0.822513347377793
1101	7	1.0167513672968305
1101	8	1.0901815293420185
1101	9	1.8804350582941964
1101	10	1.514376258179574
1101	11	1.3691302300012183
1101	12	1.5446132809969129
1101	13	1.4992480191809747
1101	14	1.7433732467384147
1101	15	1.5698800033006108
1101	16	1.5994446580132546
1101	17	1.4817063524792857
1101	18	1.5978473562042197
1101	19	1.6904524577231257
1101	20	1.7676219478954494
1101	21	1.669465005594148
1101	22	1.8461231161392888
1101	23	2.0205757454906106
1101	24	1.8413602279593988
1101	25	1.9781742340814574
1102	1	0.6917018077429304
1102	2	0.6048019522076906
1102	3	0.7313739085532234
1102	4	0.7488320042486194
1102	5	0.7366781345425153
1102	6	0.8570195827522582
1102	7	0.9779217987077082
1102	8	0.9024464283752351
1102	9	1.6055615297487051
1102	10	1.1851457354308934
1102	11	1.2270502722069168
1102	12	1.4643840949778806
1102	13	1.218540782392104
1102	14	1.334217457857548
1102	15	1.2324446145971137
1102	16	1.3075891607530608
1102	17	1.2772745920419482
1102	18	1.372084400769424
1102	19	1.208396839412842
1102	20	1.391755258356568
1102	21	1.5821956561898531
1102	22	1.549627581075221
1102	23	1.8272961294848074
1102	24	1.8191256885485316
1102	25	1.7782514277907353
1103	1	-2.0605383210663213
1103	2	-2.097367026230547
1103	3	-1.991671425804281
1103	4	-1.8386908303427667
1103	5	-3.8381164156837357
1103	6	-8.25499818268294
1103	7	-9.318074577301076
1103	8	-8.753434925303655
1103	9	-10.101993211721865
1103	10	-8.250587014912547
1103	11	-11.172990103782173
1103	12	-10.223227862223574
1103	13	-11.357714196902894
1103	14	-10.752359495330925
1103	15	-11.112905007001089
1103	16	-5.5351680269115775
1103	17	-3.864099471801353
1103	18	-3.1562413251366976
1103	19	-2.5692092583462873
1103	20	-7.633717066857251
1103	21	-4.408059943516683
1103	22	-9.14759780065021
1103	23	-10.554724738008087
1103	24	-11.643702304814052
1103	25	-12.307788820176924
1104	1	0.20935282470856365
1104	2	0.43788902434365085
1104	3	0.40570260629088395
1104	4	0.5553913901337992
1104	5	0.4790010047329787
1104	6	0.6304056253445935
1104	7	0.4406880007859364
1104	8	0.7745641769295375
1104	9	0.8966788415456364
1104	10	0.7583345427128592
1104	11	0.6557234712000621
1104	12	0.6652059087710782
1104	13	0.4968101737251658
1104	14	0.5421641271581308
1104	15	0.5998844292761731
1104	16	0.12745868913226133
1104	17	0.3465390648387583
1104	18	0.6090300622870828
1104	19	0.6363232295899692
1104	20	0.8798055456441531
1104	21	0.5069514656031302
1104	22	0.36444723731784023
1104	23	0.8507104918576189
1104	24	1.0519360447913044
1104	25	1.1800165140541736
1105	1	0.3669195451561187
1105	2	0.37582493571023434
1105	3	-0.042532110450025584
1105	4	0.2981302643100321
1105	5	0.44998707657985904
1105	6	0.43720538011627497
1105	7	0.670492649148283
1105	8	0.5054518124989329
1105	9	0.5044090214058627
1105	10	0.34021634078207796
1105	11	0.9467793798070403
1105	12	1.1363627310260185
1105	13	0.7383193000130461
1105	14	0.6019664392660431
1105	15	0.5641529072456066
1105	16	0.7688615080035532
1105	17	0.7200315143466511
1105	18	0.7335430159555543
1105	19	0.750103994846576
1105	20	0.9809603452358573
1105	21	0.9873327297833328
1105	22	0.5998336522334924
1105	23	1.1504423713821552
1105	24	1.1283445788622046
1105	25	1.3076899966255624
1106	1	-0.053587263838753785
1106	2	0.16226627596221022
1106	3	9.239259390412258E-4
1106	4	-1.0106599060484136
1106	5	-0.07917050916754675
1106	6	0.03252245671018983
1106	7	0.29773181843256324
1106	8	0.4352889819474939
1106	9	0.96322977382831
1106	10	0.4830979766314343
1106	11	0.5606968903712115
1106	12	0.7822913292829625
1106	13	0.5561746001997321
1106	14	0.1503767700228309
1106	15	0.48091674273882035
1106	16	0.3450288696446364
1106	17	0.33496991561044354
1106	18	0.3608684689249628
1106	19	-0.8551368219616791
1106	20	0.19870958507021186
1106	21	0.03046245116358648
1106	22	0.5946553552118772
1106	23	0.45864067712783196
1106	24	0.583857205227531
1106	25	0.9138710025721153
1107	1	0.009325798480194436
1107	2	-0.00833370625348806
1107	3	-0.2945328112427994
1107	4	-0.1424292123479347
1107	5	0.0915677084196247
1107	6	0.013871483645893079
1107	7	-0.1281051396002404
1107	8	-0.2657030564465579
1107	9	0.41210256848099647
1107	10	0.28325346402775153
1107	11	0.2230587958964918
1107	12	0.09880665196157423
1107	13	0.09562428252451127
1107	14	0.21040301439165887
1107	15	0.0010623172656920588
1107	16	-0.18037528486838283
1107	17	-0.2942648146254996
1107	18	-0.17813284861624368
1107	19	0.1556946943253621
1107	20	0.1864387301587982
1107	21	0.07200679857365344
1107	22	0.2952059171081345
1107	23	0.3489617902247204
1107	24	0.7298412719963139
1107	25	0.330468264541139
1108	1	-0.11052824066025835
1108	2	-0.08776791014379981
1108	3	-0.005837361147371212
1108	4	-0.2001040242107166
1108	5	-0.056928429946523806
1108	6	0.09662219317785059
1108	7	-0.26215105398173577
1108	8	0.1490134274393995
1108	9	0.3907894151924225
1108	10	0.2072086967641198
1108	11	0.40504014534221255
1108	12	0.30995545094894794
1108	13	-0.043393300023474524
1108	14	-0.05532906436391727
1108	15	-0.5260395784454701
1108	16	-0.9217051504775924
1108	17	-0.8769549543563215
1108	18	-1.0697030156405631
1108	19	-0.6869128232522428
1108	20	-0.15839627275843782
1108	21	-0.40552304132429384
1108	22	-0.2168462371989932
1108	23	-0.5526394775382997
1108	24	-0.20635400504254164
1108	25	-0.6212143398119743
1109	1	0.12947147606693932
1109	2	0.16645933370827848
1109	3	0.1381535186738425
1109	4	0.09618169679088595
1109	5	0.1775226054457022
1109	6	0.24408195939943766
1109	7	0.46573151799065116
1109	8	0.3509174726955493
1109	9	1.1135082856210374
1109	10	0.4829625640405695
1109	11	0.4161757799757524
1109	12	0.5801839918288323
1109	13	0.6805654916191344
1109	14	0.3075150668866158
1109	15	-0.015118507891259014
1109	16	0.41913787540630665
1109	17	0.32122149205268613
1109	18	0.19133663772996812
1109	19	0.5183098834133446
1109	20	0.8535337311350588
1109	21	0.7135815695131171
1109	22	0.40450752954573943
1109	23	0.7746150424710052
1109	24	0.33992508617009776
1109	25	0.6710147037264562
1110	1	0.10879160968310231
1110	2	0.08184524134043158
1110	3	0.0513575589583084
1110	4	0.3564696220410397
1110	5	0.26397822867203047
1110	6	0.43471014280046916
1110	7	0.4741422974598599
1110	8	0.3670056978944345
1110	9	-2.8445386413551716
1110	10	-1.0019671737378566
1110	11	-0.21836550127362386
1110	12	0.25406445741379
1110	13	0.09446123027552744
1110	14	0.32338510535073084
1110	15	0.539072316718233
1110	16	0.16427705504159462
1110	17	0.2855942385565484
1110	18	0.2621623149429979
1110	19	0.21733854624491755
1110	20	0.4476652043104181
1110	21	0.4106382826866337
1110	22	0.640836839480869
1110	23	0.781189078423651
1110	24	0.7676563880456584
1110	25	0.7625179261878472
1111	1	0.11182007780773162
1111	2	0.3291826621445928
1111	3	0.16870938814082592
1111	4	0.10902079624960237
1111	5	0.2031720759711746
1111	6	0.12688287208285942
1111	7	0.14691701560523995
1111	8	0.30234318510470004
1111	9	0.8681046451181658
1111	10	0.47743557320330865
1111	11	0.6524134511350397
1111	12	0.8633579925168462
1111	13	0.5926796242437575
1111	14	0.4411593997202843
1111	15	0.49217212772294516
1111	16	0.43610653463613147
1111	17	0.4715728505465222
1111	18	0.41287709807899375
1111	19	0.5628412361292519
1111	20	0.7729267002128992
1111	21	0.7450472202500364
1111	22	0.702259869993604
1111	23	0.978429205538923
1111	24	0.8722827095054058
1111	25	1.2705500073291276
1112	1	0.021536878334689646
1112	2	-0.09477502741937727
1112	3	-0.11153208086267696
1112	4	-0.015129646882407144
1112	5	0.24068663527318535
1112	6	0.07766357433217763
1112	7	0.3904784472214651
1112	8	-0.0536866949786976
1112	9	0.5678884479575501
1112	10	0.15022687842969873
1112	11	0.40020457047473457
1112	12	-0.08478311866570465
1112	13	0.26823779825032545
1112	14	-0.550552240592026
1112	15	0.05272285970101365
1112	16	-0.09364517759901503
1112	17	-0.22524463802363215
1112	18	-0.3205517591464684
1112	19	-0.06106318049487669
1112	20	-0.06414033140401898
1112	21	-0.20768157456122793
1112	22	0.1697920251114553
1112	23	0.31977622363204716
1112	24	0.17192856254819588
1112	25	0.10659663033647604
1113	1	-2.364613193562249
1113	2	-2.147120781247459
1113	3	-2.1074819310884685
1113	4	-2.0614610425866644
1113	5	-2.0267768780284072
1113	6	-1.9678502429034204
1113	7	-2.1340130248184686
1113	8	-2.299751766357339
1113	9	-10.885287110805237
1113	10	-4.27055651909258
1113	11	-3.1150410353067883
1113	12	-13.090830435089305
1113	13	-5.088008464039266
1113	14	-4.544052013339233
1113	15	-3.8559970027246635
1113	16	-3.315780396997635
1113	17	-3.618734310646719
1113	18	-3.9656599766606107
1113	19	-4.927377405988121
1113	20	-15.362283237528683
1113	21	-18.376926165014485
1113	22	-12.940506646190634
1113	23	-19.15973726933641
1113	24	-20.270882142520207
1113	25	-16.571195588846848
1114	1	-0.20829656369625837
1114	2	-0.12604743108594008
1114	3	-0.18850015928077468
1114	4	-0.7411749985628688
1114	5	-0.23732751804400465
1114	6	-0.14356284981422007
1114	7	-0.30953117659886686
1114	8	0.05123500120817681
1114	9	0.42625846494531316
1114	10	-0.2545985509115525
1114	11	-0.3137130490503921
1114	12	-0.3374754253665486
1114	13	-0.41497396274553466
1114	14	-0.45097018936064615
1114	15	-0.36714365561962126
1114	16	-0.3663851050251026
1114	17	-0.3727401390308458
1114	18	-0.3152787075905721
1114	19	-0.6154297455099424
1114	20	-0.8694401348890466
1114	21	-0.8904178730050631
1114	22	-1.0860686952945073
1114	23	-0.5359995150033257
1114	24	-0.34284371523536095
1114	25	-0.6921946350154506
1115	1	-0.1179946655104942
1115	2	-0.16513960643240821
1115	3	-0.14763135820858153
1115	4	-0.17333852449672094
1115	5	-0.40230919948906774
1115	6	-0.39274210818616595
1115	7	-0.6094109529851295
1115	8	-0.3864534107137061
1115	9	-0.030247615374861425
1115	10	-0.4182428537495966
1115	11	-0.3101816167665916
1115	12	-0.28562326440982133
1115	13	-0.45887371894361095
1115	14	-0.5115212433491791
1115	15	-0.4492834122977172
1115	16	-0.41389306970314266
1115	17	-0.37248226374320836
1115	18	-0.3667948533655192
1115	19	-0.25590700161869506
1115	20	-0.5617102310924835
1115	21	-0.13754108393559505
1115	22	-0.6426546610422363
1115	23	-0.3766029949969223
1115	24	-0.1141836112288388
1115	25	-0.11647448656874815
1116	1	-0.5554706967148313
1116	2	-0.6077478590876524
1116	3	-0.48253065285850383
1116	4	-0.2964260775517005
1116	5	-1.320471707788812
1116	6	-1.1777493601795896
1116	7	-2.2090445506696668
1116	8	-1.4603559163057511
1116	9	-0.6937417708992406
1116	10	-1.5409795386959253
1116	11	-1.2793308438588937
1116	12	-2.0804612960316398
1116	13	-1.7967841146181946
1116	14	-1.287213769615743
1116	15	-2.370109120115469
1116	16	-2.7084663995197005
1116	17	-3.3135296792125786
1116	18	-2.5952598849490442
1116	19	-2.2012224134396874
1116	20	-2.565745349187516
1116	21	-1.691078406215908
1116	22	-1.6736259858673534
1116	23	-1.6528818296940848
1116	24	-2.0835612666747387
1116	25	-1.591251762181713
1201	1	0.6012072096973782
1201	2	0.6894632991777883
1201	3	0.36518810551998016
1201	4	0.6234206883242379
1201	5	0.8985662553374141
1201	6	0.9124707327173951
1201	7	1.07856532066101
1201	8	0.7947853011132793
1201	9	1.6237057601811529
1201	10	1.315988459429711
1201	11	1.4219646656373257
1201	12	1.4593152053318619
1201	13	1.242249041826021
1201	14	1.1837794744598398
1201	15	1.2735158669016613
1201	16	1.1856839409334796
1201	17	1.182563985870381
1201	18	1.2702605048028204
1201	19	1.3345940099541238
1201	20	1.5062793991863046
1201	21	1.531961300842184
1201	22	1.3827414185485125
1201	23	1.840181693386441
1201	24	1.7350058106935151
1201	25	1.9513344742828878
1202	1	0.8122186702261374
1202	2	0.81563385542022
1202	3	0.716902380252364
1202	4	0.87472016750759
1202	5	0.8764763904422495
1202	6	0.7362239437794642
1202	7	0.9703956100249478
1202	8	0.9043528247037713
1202	9	1.7040713568140973
1202	10	1.302900458712223
1202	11	1.4155761735712886
1202	12	1.3764848583129492
1202	13	1.2177075590680602
1202	14	1.2356074576245888
1202	15	1.1555566610029118
1202	16	1.0301910228868323
1202	17	1.173634190417438
1202	18	1.0242761741451005
1202	19	1.1010810920355922
1202	20	1.4108696964938119
1202	21	1.3871662642953382
1202	22	1.4626858135235636
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2211	3	0.15735257118155133
2211	4	0.17165190194853608
2211	5	-0.6364866965812652
2211	6	0.09169389755449942
2211	7	0.23373050652122984
2211	8	0.24459533826979651
2211	9	0.7987119409904224
2211	10	0.06622195666894726
2211	11	0.43815271272852385
2211	12	0.5595959991985922
2211	13	0.3902740493206238
2211	14	0.4009253379756288
2211	15	0.3289069658196908
2211	16	0.4297488710813795
2211	17	0.3320652214350517
2211	18	0.2676472466981963
2211	19	-0.011721212513208457
2211	20	0.4611544154594469
2211	21	0.5933898859205797
2211	22	0.3409734801678965
2211	23	0.7471897756156878
2211	24	0.7897385190584529
2211	25	0.9157130073469304
2212	1	0.14973672387250403
2212	2	0.18910751487801036
2212	3	0.22078774268248935
2212	4	0.358099345611258
2212	5	-0.02174386476917789
2212	6	0.2541087749424875
2212	7	0.3881640269215616
2212	8	0.10564078453649728
2212	9	0.7241338024717194
2212	10	0.30093317373387407
2212	11	0.5353681955651695
2212	12	0.3850730958270816
2212	13	0.13089807928886898
2212	14	0.17407613100998987
2212	15	0.22484811616672573
2212	16	0.32926812774240943
2212	17	0.2658268865108795
2212	18	0.3437812248114369
2212	19	0.5558463111687573
2212	20	0.7970191567351463
2212	21	0.877912055890576
2212	22	0.6203181331431438
2212	23	0.9834136671307192
2212	24	1.0397866231614117
2212	25	1.0529910214580127
2213	1	-0.1422008106235495
2213	2	-0.1789773019197689
2213	3	-0.382440013941288
2213	4	-0.3460007718632383
2213	5	-0.1912388049903342
2213	6	0.029462970199261918
2213	7	-0.11574583878812561
2213	8	-0.25703768219588596
2213	9	0.3560268384034515
2213	10	-0.09895794223084664
2213	11	0.11183654617605754
2213	12	0.1331840007672369
2213	13	-0.13909473157290364
2213	14	-0.07128180109533844
2213	15	-0.07463224175464234
2213	16	-0.2636559132942011
2213	17	-0.3987694801555719
2213	18	-0.4801239483614239
2213	19	-0.4200342374607402
2213	20	0.0196872773365655
2213	21	-0.22489369456096142
2213	22	-0.09496622170284041
2213	23	-0.501257737172665
2213	24	-0.395999715006198
2213	25	-0.022648887010674912
2214	1	-0.9175232317312485
2214	2	-0.7003149973898815
2214	3	-0.8011770805605103
2214	4	-0.6068208620500712
2214	5	-0.7812248205241588
2214	6	-0.5443470528663674
2214	7	-0.44482571810128135
2214	8	-0.3916694104662177
2214	9	-9.79433344295914
2214	10	-3.2653577338141098
2214	11	-1.205465352025925
2214	12	-0.60800091401277
2214	13	-0.5086030089365998
2214	14	-0.6163948099848184
2214	15	-0.7706767452928531
2214	16	-0.7334217198182387
2214	17	-0.5811002315783753
2214	18	-0.9875862575510368
2214	19	-1.0320496261981873
2214	20	-0.9445639050683141
2214	21	-1.3484415022668728
2214	22	-1.0810633006915147
2214	23	-1.5485094533277177
2214	24	-1.6963411456684518
2214	25	-8.968911020825232
2215	1	-0.4503588132562051
2215	2	-0.24370427155788477
2215	3	-0.19492032295490702
2215	4	-0.1941449638314694
2215	5	-0.3554198218005027
2215	6	-0.20506800668945147
2215	7	-0.36102512710732526
2215	8	-0.23853328004659957
2215	9	0.5684661470070651
2215	10	0.30170885391080304
2215	11	0.4710813294133658
2215	12	0.40100245526757305
2215	13	0.35034469571610316
2215	14	0.04688030157831946
2215	15	-0.31191267376948417
2215	16	0.023452694633981253
2215	17	0.013552222008819115
2215	18	0.01691943654610384
2215	19	-0.16665952608205004
2215	20	0.2364213788881102
2215	21	0.26030192055308277
2215	22	0.05132108327546803
2215	23	0.4317756457927402
2215	24	0.6856579963077536
2215	25	0.7736370692699381
2216	1	-0.568593789723618
2216	2	-0.5857849864826825
2216	3	-0.4743900588287353
2216	4	-0.405562636831732
2216	5	-1.1658223375903667
2216	6	-1.5856166945292713
2216	7	-1.0955113543225394
2216	8	-1.9493955945520973
2216	9	-0.5820725348503437
2216	10	-1.0493216563943015
2216	11	-0.5998229678649061
2216	12	-1.8458604839500552
2216	13	-1.4252359424432157
2216	14	-1.538799231687598
2216	15	-1.2023008423570545
2216	16	-2.2002156891086777
2216	17	-2.6590935926846235
2216	18	-2.125739137098453
2216	19	-3.5173408858368234
2216	20	-2.2208790176452666
2216	21	-2.357519316482815
2216	22	-2.422397065670726
2216	23	-1.982979737055203
2216	24	-2.115721664547678
2216	25	-2.129130193696806
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	67272.0
3	3.0
4	908.0
5	9420.0
6	13308.0
7	10480.0
8	8171.0
9	6264.0
10	5410.0
11	4786.0
12	4307.0
13	3769.0
14	3889.0
15	3758.0
16	3941.0
17	4216.0
18	4866.0
19	6249.0
20	8604.0
21	11200.0
22	13495.0
23	15883.0
24	18115.0
25	20165.0
26	22470.0
27	24484.0
28	26661.0
29	31218.0
30	37326.0
31	46223.0
32	57367.0
33	70953.0
34	89494.0
35	112493.0
36	147894.0
37	223181.0
38	495350.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.73150199433968	19.657318926571314	13.604311251700729	22.00686782738828
2	15.25866243219634	22.690512905115582	40.15458102066814	21.896243642019936
3	17.96359789836721	29.97067190177199	30.146664977133142	21.919065222727653
4	11.272732392745224	18.637332822370382	37.994829561002426	32.09510522388197
5	11.339461878465219	40.343867229552224	33.941656348322795	14.375014543659761
6	26.7538333633317	39.54275135268167	19.526735895561586	14.176679388425036
7	24.96477974252017	34.832758115047326	22.880401740532776	17.32206040189972
8	25.885304984306558	35.05381571744697	20.697161219373903	18.36371807887257
9	26.799493220383614	15.930552332221104	20.76933015810226	36.50062428929303
10	15.161452305280498	32.28592949130123	31.519972565661764	21.03264563775651
11	31.960733280871562	24.735277276805263	23.915281379846718	19.38870806247646
12	22.84441620185777	29.27962425932082	30.066417013628065	17.809542525193347
13	27.97683324885464	23.564413166922552	27.632001018641635	20.826752565581177
14	21.60289048233271	22.975771641121298	30.69655996116312	24.724777915382866
15	23.064024698205486	29.46514770009287	27.066096350182644	20.404731251519
16	20.25334124297728	30.06589125219092	28.215267204546947	21.465500300284855
17	19.86200774446332	28.88244027120927	29.67788542479759	21.577666559529824
18	19.11488658278997	26.57293292925582	33.67526861970796	20.63691186824624
19	20.77635355544018	26.576535760116766	33.86882487779552	18.77828580664753
20	21.358098688774103	25.329061558495784	33.873182653883994	19.43965709884612
21	22.15396671370542	25.09769469298796	33.417309240512914	19.331029352793706
22	21.185816533745392	25.47864209087297	33.54483192657383	19.79070944880781
23	20.389233929101632	25.531860186780865	34.0281548732296	20.0507510108879
24	20.071747780838688	25.80593816957453	34.09874502601775	20.023569023569024
25	20.173360991019422	25.23974250163158	34.282641533714695	20.3042549736343
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2663.0
1	2663.0
2	3442.0
3	4221.0
4	4221.0
5	4221.0
6	13573.0
7	22925.0
8	22925.0
9	22925.0
10	19893.0
11	16861.0
12	16861.0
13	16861.0
14	15655.5
15	14450.0
16	14450.0
17	14450.0
18	23481.5
19	32513.0
20	32513.0
21	32513.0
22	47078.0
23	61643.0
24	61643.0
25	61643.0
26	87540.5
27	113438.0
28	113438.0
29	113438.0
30	128105.0
31	142772.0
32	142772.0
33	142772.0
34	151574.5
35	160377.0
36	160377.0
37	160377.0
38	163253.5
39	166130.0
40	166130.0
41	166130.0
42	173696.0
43	181262.0
44	181262.0
45	181262.0
46	187341.0
47	193420.0
48	193420.0
49	193420.0
50	185672.5
51	177925.0
52	177925.0
53	177925.0
54	156370.5
55	134816.0
56	134816.0
57	134816.0
58	113508.0
59	92200.0
60	92200.0
61	92200.0
62	75696.0
63	59192.0
64	59192.0
65	59192.0
66	44511.0
67	29830.0
68	29830.0
69	29830.0
70	22051.0
71	14272.0
72	14272.0
73	14272.0
74	10350.5
75	6429.0
76	6429.0
77	6429.0
78	4975.0
79	3521.0
80	3521.0
81	3521.0
82	2502.0
83	1483.0
84	1483.0
85	1483.0
86	1085.5
87	688.0
88	688.0
89	688.0
90	604.0
91	520.0
92	520.0
93	520.0
94	280.0
95	40.0
96	40.0
97	40.0
98	21.0
99	2.0
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.02883215097028452
2	9.794361263790918E-4
3	3.672885473921595E-4
4	0.004652321600300687
5	0.03544334482334339
6	0.010467723600676545
7	0.018364427369607975
8	0.026138701622742015
9	0.032811110233699585
10	0.03691249901291203
11	0.04027931069734016
12	0.00771305949523535
13	0.002754664105441196
14	0.005754187242477166
15	0.00697848240045103
16	0.008814925137411828
17	0.010284079326980467
18	0.014875186169382458
19	0.016221910843153713
20	0.014446682864091608
21	0.011508374484954332
22	0.010406508842777853
23	0.007835489011032735
24	0.005692972484578472
25	0.10639124922792886
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1633593.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	47.25613983174659
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.79965513582628	35.81999102296775
2	12.709355436699724	12.011901553761017
3	4.691508823678533	6.651077909808771
4	2.1350483880713753	4.0357658069698426
5	1.206289430422955	2.8502291000812554
6	0.7165796512687821	2.0317672920565064
7	0.48277193482127967	1.5969756641130068
8	0.34547949560612073	1.3060821882691298
9	0.2617505511850797	1.1132388583054982
>10	1.4450608339614852	12.63458969019797
>50	0.12514229936159033	4.087754300446867
>100	0.07448097446790385	6.156217473864099
>500	0.004281933448214481	1.4286970141369737
>1k	0.0018165778265152345	1.8044504606018394
>5k	2.5951111807360496E-4	1.0956205256909635
>10k+	5.190222361472099E-4	5.375641138728512
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	28160	1.7238075824272017	No Hit
TATCAACGCAGAGTACTTTTTTTTT	20759	1.2707571592189733	No Hit
GGTATCAACGCAGAGTACTTTTTTT	19602	1.199931684330185	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	19148	1.1721401842441783	No Hit
GAGTACTTTTTTTTTTTTTTTTTTT	9531	0.5834378575324454	No Hit
ACGCAGAGTACTTTTTTTTTTTTTT	8337	0.5103474366014056	No Hit
GTGGTATCAACGCAGAGTACTTTTT	4218	0.2582038488166881	No Hit
GTGGTATCAACGCAGAGTACATGGG	3274	0.20041711736032167	No Hit
ATCAACGCAGAGTACTTTTTTTTTT	3217	0.19692787616009616	No Hit
GCAGAGTACTTTTTTTTTTTTTTTT	2994	0.1832769851486876	No Hit
GTATAACGCAGAGTACTTTTTTTTT	2788	0.17066674502155676	No Hit
GGTATCAACGCAGAGTACATGGGAT	2175	0.1331420984296578	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA	1750	0.10712582632271318	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	1.8364427369607975E-4	0.0	0.0	0.0	0.0
3	1.8364427369607975E-4	0.0	0.0	0.0	0.0
4	1.8364427369607975E-4	0.0	0.0	0.0	0.0
5	1.8364427369607975E-4	0.0	0.0	6.121475789869325E-5	0.0
6	2.44859031594773E-4	0.0	0.0	6.121475789869325E-5	0.0
7	2.44859031594773E-4	0.0	0.0	6.121475789869325E-5	0.0
8	2.44859031594773E-4	0.0	6.121475789869325E-5	6.121475789869325E-5	0.0
9	2.44859031594773E-4	0.0	6.121475789869325E-5	6.121475789869325E-5	0.0
10	2.44859031594773E-4	0.0	6.121475789869325E-5	6.121475789869325E-5	0.0
11	2.44859031594773E-4	0.0	6.121475789869325E-5	1.224295157973865E-4	0.0
12	2.44859031594773E-4	0.0	6.121475789869325E-5	1.224295157973865E-4	0.0
13	3.0607378949346623E-4	0.0	6.121475789869325E-5	1.224295157973865E-4	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAACGCA	425	0.0	14.754938	4
ATAACGC	425	0.0	14.530934	3
TATAACG	410	0.0	14.366918	2
TATCACG	210	0.0	13.572434	2
GGTATCA	4780	0.0	12.720998	1
AACCGTG	60	4.0907215E-4	12.667218	7
ACCGACC	55	0.0030675603	12.091435	8
ATCACGC	245	0.0	12.0216675	3
TTAGTAC	80	2.8634975E-5	11.876244	3
CCTATAC	80	2.8634975E-5	11.876244	3
GTATAAC	535	0.0	11.720854	1
TACAACG	155	2.5465852E-11	11.646025	2
GTGGTAT	1575	0.0	11.6424885	1
TGGTATC	1535	0.0	11.574148	2
TCACGCA	275	0.0	11.401545	4
GGTATCC	125	1.82863E-8	11.401195	3
CGCTATT	75	2.0748693E-4	11.399448	11
TTGCGCG	210	0.0	11.3069	18
GCGTGCG	60	0.005875311	11.083476	9
AACGCCG	165	8.0035534E-11	10.93987	6
>>END_MODULE