FastQCFastQC Report
Thu 26 May 2016
SRR1364314_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364314_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4565017
Sequences flagged as poor quality0
Sequence length25
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNN120930.26490591382244577No Hit
GTATCAACGCAGAGTACTTTTTTTT65440.14335105433342307No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGACCG4950.013.24669555
CGTCCGA655.5162294E-513.13871711
GGACCGA4750.012.3993626
ACCGATT701.09265355E-412.2132538
ACCGAGT4050.011.7274048
GGTATCA31600.011.4159291
CGCATAT2050.010.6576378
GTATCAA69850.010.2611311
CGCGCCA2800.010.17759910
CGAGTGG4650.010.00979610
CCGTATG1151.0287E-59.9120969
TGGTATC11050.09.8800652
CAGGACC12300.09.8124314
ACGCATA5600.09.6690397
TTGCGCG1901.038643E-99.50798118
GGACCGG1502.6539965E-79.4995116
GACCGAG5800.09.4994077
GACCGAT900.00111501929.4994067
GTACTAG3000.09.4932451
GTGGTAT12650.09.4557241
Produced by FastQC (version 0.11.5)