FastQCFastQC Report
Thu 26 May 2016
SRR1364314_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364314_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4565017
Sequences flagged as poor quality0
Sequence length25
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT125200.2742596577405955No Hit
TATCAACGCAGAGTACTTTTTTTTT90490.19822489160500387No Hit
GGTATCAACGCAGAGTACTTTTTTT89890.19691054819730136No Hit
GTGGTATCAACGCAGAGTACATGGG51700.11325259029703504No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGACCGA7150.013.6868856
AGGACCG7500.013.43135
TGGTATC14450.012.4258062
ACCGAGT7250.012.3186558
CGAGTGG7450.012.24207710
CCGAGTG7400.011.9402979
GTGGTAT15450.011.7464571
GGTATCA44200.011.7374121
CGTCGCT752.0821614E-411.39606312
ACGGCCC4300.011.26466215
TCGCGGT1303.2838216E-810.95883417
CAGGACC15450.010.7623424
GACCGAG8700.010.7023777
TAGACCG909.490179E-510.5591975
TGCCGTA1909.094947E-1210.49740815
TCGCGCA2300.010.32308718
CTGTCCG2500.010.2609539
TAACGCA1502.4427209E-810.1350534
TTAAACG1606.21003E-910.09125917
TATAACG1054.108216E-59.9525412
Produced by FastQC (version 0.11.5)