FastQCFastQC Report
Thu 26 May 2016
SRR1364313_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364313_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3491740
Sequences flagged as poor quality0
Sequence length25
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNN91760.262791616787046No Hit
GTATCAACGCAGAGTACTTTTTTTT64170.18377656984769772No Hit
TATCAACGCAGAGTACTTTTTTTTT42600.12200221093208544No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGTCC1001.4439684E-712.3502898
AGGACCG3250.012.2828635
GGTATCA25150.012.2686921
GGACCGA2700.011.9635886
TGGACCG1501.7462298E-1011.4055155
TAAGGCG752.0659211E-411.4055155
GTATCAA62350.010.917811
ATAACGC2100.010.8503773
TATAACG2250.010.5489782
TAACGCA2200.010.368654
CATGGGT20200.010.2103824
ATGGGTA10450.010.1867445
CGTGCGA750.002674161210.12178211
GAACGCG750.002674161210.12178211
TATCACG951.6555323E-49.9937682
GTCTAGA4000.09.9687831
CAGGACC9450.09.8566194
CTCAACG1554.1080057E-89.8003413
GTATTAG3400.09.7733181
GTGGTAT9950.09.7326241
Produced by FastQC (version 0.11.5)