##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1364313_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3491740
Sequences flagged as poor quality	0
Sequence length	25
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	27.000450491731915	31.0	27.0	31.0	10.0	34.0
2	26.890909689724893	31.0	26.0	31.0	10.0	34.0
3	26.264710144512478	31.0	25.0	31.0	10.0	34.0
4	30.18373618883422	35.0	30.0	37.0	10.0	37.0
5	30.709944898531965	35.0	31.0	37.0	16.0	37.0
6	31.041276269138024	35.0	32.0	37.0	15.0	37.0
7	30.687378785362025	35.0	31.0	37.0	10.0	37.0
8	30.22067994753332	35.0	30.0	37.0	10.0	37.0
9	31.44292788122827	37.0	30.0	39.0	10.0	39.0
10	30.697783340111233	35.0	29.0	39.0	8.0	39.0
11	31.418507964510532	37.0	30.0	39.0	8.0	39.0
12	30.90001632423949	37.0	30.0	39.0	7.0	39.0
13	30.54288291797213	35.0	29.0	39.0	2.0	39.0
14	30.896223659264436	36.0	27.0	40.0	2.0	41.0
15	31.62644011295228	37.0	30.0	40.0	2.0	41.0
16	32.01195363915985	37.0	31.0	40.0	2.0	41.0
17	32.179137621930614	38.0	31.0	40.0	2.0	41.0
18	32.0149177773832	38.0	31.0	40.0	2.0	41.0
19	31.983234719652668	38.0	31.0	40.0	2.0	41.0
20	31.831263496136597	38.0	30.0	40.0	2.0	41.0
21	31.7567696907559	38.0	30.0	40.0	2.0	41.0
22	31.852610159977548	38.0	31.0	40.0	2.0	41.0
23	31.867074581727163	38.0	31.0	40.0	2.0	41.0
24	31.697619238545826	38.0	31.0	40.0	2.0	41.0
25	31.520082251255822	38.0	31.0	40.0	2.0	41.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1101	1	0.6647705563072748
1101	2	0.806681909544352
1101	3	1.4485929773193718
1101	4	0.9127437811525141
1101	5	0.36732637152144676
1101	6	0.24799400433291652
1101	7	0.5432433392493827
1101	8	0.9070295035282108
1101	9	0.8158781125714505
1101	10	1.1307369321359246
1101	11	0.4911502820851652
1101	12	0.9153615866418292
1101	13	0.8455962982434926
1101	14	1.3812800271473478
1101	15	0.6496079015894161
1101	16	0.028193634982965676
1101	17	-0.40658846748383226
1101	18	-6.489015996825781
1101	19	-7.744210667811593
1101	20	-8.918243930165438
1101	21	-10.734256082506892
1101	22	-11.021013143117209
1101	23	-12.595496116281755
1101	24	-12.132860167409426
1101	25	-12.614906393519174
1102	1	1.721904097610743
1102	2	2.0143531245262096
1102	3	2.6810473801859835
1102	4	2.147008672505468
1102	5	1.4092167564991378
1102	6	1.415155851089466
1102	7	1.803470938198636
1102	8	2.0689102526069796
1102	9	2.2337759520266296
1102	10	-1.5955092513861615
1102	11	0.7916668245602843
1102	12	1.9888591598456067
1102	13	2.469961040896692
1102	14	3.138419801585144
1102	15	2.624843852060753
1102	16	2.258206475239678
1102	17	2.0216970044495923
1102	18	2.2302842627359816
1102	19	1.9779064768369778
1102	20	2.2883537810627566
1102	21	2.4192390466059024
1102	22	2.1836966834102753
1102	23	2.238165021389925
1102	24	2.4109595570017603
1102	25	2.5466057402863775
1103	1	-0.3520652208157635
1103	2	-0.07279015250308518
1103	3	-2.392831999781521
1103	4	-0.15737082403767033
1103	5	-2.5741033055097837
1103	6	-7.153070268781079
1103	7	-8.825857020974166
1103	8	-2.5806338059956246
1103	9	-7.887556700961422
1103	10	-4.612362786808241
1103	11	-9.684643405595065
1103	12	-2.9204853042498087
1103	13	-0.3078330320373226
1103	14	0.20178509210560946
1103	15	-0.07408695386683917
1103	16	-0.2392794004722596
1103	17	-0.37174506394050155
1103	18	-0.12239887090629153
1103	19	-6.298330992294783
1103	20	-1.7286520825601563
1103	21	-6.3750168027868845
1103	22	-2.439858401566376
1103	23	-1.1647483364766238
1103	24	-7.072689570382497
1103	25	-3.8062104779675217
1104	1	1.5205635672767173
1104	2	1.6047966473113462
1104	3	2.4654349307160075
1104	4	1.8501475954097906
1104	5	1.3661894571262572
1104	6	1.2731768211872847
1104	7	1.423114852871148
1104	8	1.7570717017037438
1104	9	1.8786701075593477
1104	10	2.277613460811871
1104	11	1.7592738305745677
1104	12	2.1302439216202593
1104	13	2.3576851261399625
1104	14	2.8851252372685963
1104	15	1.88090675744402
1104	16	1.1152708317201068
1104	17	0.9808860527774499
1104	18	0.8985675606772325
1104	19	1.410064682783542
1104	20	-9.836373816617925
1104	21	-3.661037227873063
1104	22	-0.9972065052670303
1104	23	0.11799680886941388
1104	24	0.6617124900109275
1104	25	1.190605466795244
1105	1	1.3694962844235299
1105	2	1.2966730109096716
1105	3	2.1150491503183524
1105	4	1.4873381091305617
1105	5	1.2348196450357918
1105	6	1.255523817559606
1105	7	1.5194839146430823
1105	8	1.7965105962049357
1105	9	1.7350422430222174
1105	10	2.2492214477414407
1105	11	1.7333178382652328
1105	12	2.059397155975919
1105	13	2.078586043459687
1105	14	2.8065660821956584
1105	15	2.1589230077744226
1105	16	1.4736089827823626
1105	17	0.9593841966945789
1105	18	1.5070443094524855
1105	19	1.4498643537698683
1105	20	1.3837649138592063
1105	21	1.2468937597191214
1105	22	1.3261904381806744
1105	23	1.334772684096876
1105	24	1.3962833862613806
1105	25	1.3324605277842672
1106	1	0.7883020188403478
1106	2	1.0939714997403662
1106	3	1.7382935166190592
1106	4	1.2153104471837928
1106	5	0.5424675295131145
1106	6	0.4045636428989816
1106	7	0.8891099998828587
1106	8	0.8834155062135771
1106	9	1.185063797302842
1106	10	-2.2440392058526797
1106	11	-0.1521921467604308
1106	12	0.5575158923012786
1106	13	1.4833219439360903
1106	14	0.6286632220824622
1106	15	1.1994902098056066
1106	16	0.6483362546579592
1106	17	-6.386178487135055
1106	18	-1.934252824262174
1106	19	-0.33697083719940224
1106	20	0.3472534848551767
1106	21	0.6705004363245948
1106	22	0.71080119156926
1106	23	0.25779758800501185
1106	24	0.5200029593529862
1106	25	0.5948813800387356
1107	1	0.7855671442524184
1107	2	1.1407204948536993
1107	3	1.680319743635799
1107	4	1.0865077624582327
1107	5	0.5806777350126922
1107	6	0.7032136245950689
1107	7	0.7215274131183307
1107	8	0.8738169147471275
1107	9	1.022371844707191
1107	10	-2.104449857249424
1107	11	0.08923691836143988
1107	12	0.41462311106678484
1107	13	1.3836809393506044
1107	14	1.8647049927447057
1107	15	1.5097226641406145
1107	16	-5.4375584447354335
1107	17	-1.7380755855811891
1107	18	-0.0320452562095177
1107	19	0.7168425606017905
1107	20	1.1873472596724532
1107	21	1.2524048967003907
1107	22	0.9178556099914772
1107	23	0.6034873643136507
1107	24	0.8776214836088414
1107	25	1.1462789754039129
1108	1	-0.4979527562450947
1108	2	-3.305771581800066
1108	3	-0.23532425857536765
1108	4	-0.7119006584004346
1108	5	-0.9660538846666888
1108	6	-0.5538759276437375
1108	7	-0.15604997770532947
1108	8	0.07078807745212146
1108	9	-0.5437166940803522
1108	10	-3.5387643956684407
1108	11	-1.5229521363649283
1108	12	-6.070087594663821
1108	13	-1.8278401588620348
1108	14	-6.251818869425922
1108	15	-2.783551704755645
1108	16	-7.846059918846361
1108	17	-3.440712862161252
1108	18	-2.008100166841544
1108	19	-0.756215758911754
1108	20	-0.6369824805154956
1108	21	-0.22105650001971
1108	22	-0.31892806423000764
1108	23	-0.39132791116083254
1108	24	-0.11576344360693369
1108	25	-0.26330965551556673
1109	1	-9.197868543086386
1109	2	-6.380780088524823
1109	3	-5.397780973353427
1109	4	-5.353316689852768
1109	5	-2.4830239806252763
1109	6	-1.5044980459480222
1109	7	-0.9720673204914902
1109	8	-0.44606880049106934
1109	9	-5.611586248068331
1109	10	-1.441528022143281
1109	11	-1.0243846824788925
1109	12	-6.284074358336756
1109	13	-8.411062779507205
1109	14	-8.569209552888303
1109	15	-4.084047212931665
1109	16	-2.165902691341696
1109	17	-1.8396380426131422
1109	18	-1.1692262795484716
1109	19	-0.854297851197078
1109	20	-0.600833649045672
1109	21	-0.5163307923558698
1109	22	-0.7050649726197555
1109	23	-1.0826694660671272
1109	24	-0.7476167207116156
1109	25	-0.5954488473972148
1110	1	-2.8366097794949496
1110	2	-2.735484854016036
1110	3	-1.9801980305805564
1110	4	-2.7858038675957886
1110	5	-5.981653420950234
1110	6	-4.5925830445440425
1110	7	-9.684592485936431
1110	8	-10.61213238903916
1110	9	-12.247068833099235
1110	10	-9.685329078510932
1110	11	-12.655759942169652
1110	12	-7.188570484113605
1110	13	-5.344714715289765
1110	14	-5.003491103536028
1110	15	-10.468034012558782
1110	16	-12.299519182236242
1110	17	-13.157088816675419
1110	18	-9.616401972080634
1110	19	-12.958385260883482
1110	20	-14.071952317601074
1110	21	-14.814215615063013
1110	22	-15.717065594312878
1110	23	-16.490589846923562
1110	24	-16.884971390287546
1110	25	-17.553084480888167
1111	1	-0.7776730558215164
1111	2	-5.956374395061914E-4
1111	3	-2.8181807905974416
1111	4	-3.6783310957392565
1111	5	-1.2589992406976336
1111	6	-0.6717272484791295
1111	7	-0.2873403142945392
1111	8	0.15257013627172
1111	9	-4.402395825281996
1111	10	-4.284574401146866
1111	11	-1.45717961134455
1111	12	-6.346031621174063
1111	13	-1.451439819466799
1111	14	0.06494745561850834
1111	15	0.0800675312042145
1111	16	-0.12205522209734099
1111	17	-0.08764611220468055
1111	18	0.12348162874631541
1111	19	0.361617690225728
1111	20	0.3183681722190421
1111	21	0.41073674948923866
1111	22	0.011238359693347633
1111	23	-0.07610707042271514
1111	24	0.20539397431687334
1111	25	0.3875820921026545
1112	1	-0.19899801417352947
1112	2	0.3143201225190815
1112	3	-2.5116304963963074
1112	4	-0.4395931678760121
1112	5	-0.5763364924313095
1112	6	-0.23624171706106978
1112	7	0.23368347483112828
1112	8	-3.5885084278263655
1112	9	-0.7818612474969129
1112	10	-3.5256393822848544
1112	11	-0.9591777514744564
1112	12	0.29621414627281695
1112	13	0.718570899190599
1112	14	1.4345395108391799
1112	15	0.8099958016149422
1112	16	0.47061575943368794
1112	17	0.26932348765351577
1112	18	0.3442328595615898
1112	19	0.5987225159977712
1112	20	0.6036000266045711
1112	21	0.44290739642347177
1112	22	0.37299092761136166
1112	23	0.1553521569348426
1112	24	0.42051302912267374
1112	25	0.5151087795686955
1113	1	-0.9688060820768172
1113	2	-0.438386865976625
1113	3	-2.999796350463015
1113	4	-1.1711852277301738
1113	5	-1.3653089429457061
1113	6	-1.1853874666768789
1113	7	-0.8103590688074291
1113	8	-8.886245478098715
1113	9	-9.6398964461026
1113	10	-3.375333152170427
1113	11	-9.40300457686513
1113	12	-7.500412322376608
1113	13	-2.6211847799953425
1113	14	-0.8948195488170185
1113	15	-7.2361747913143155
1113	16	-2.7841660630895824
1113	17	-1.9102997605285914
1113	18	-1.2964220708350886
1113	19	-0.959805650846107
1113	20	-6.875330657836571
1113	21	-2.486500439638828
1113	22	-1.7468175994840358
1113	23	-1.5808496304449235
1113	24	-7.524937346496468
1113	25	-4.501343614454868
1114	1	-4.546036997202467
1114	2	-4.4013171646963976
1114	3	-1.2021576591401129
1114	4	-0.8416925771848902
1114	5	-0.9033743768006133
1114	6	-0.962570965965643
1114	7	-0.7162291494143531
1114	8	-0.34946975500720256
1114	9	-0.7450078733192917
1114	10	-3.8550296908143338
1114	11	-1.6738153876759405
1114	12	-5.0852771872414735
1114	13	-1.6855280706544242
1114	14	-6.516422204103883
1114	15	-2.5969334447045505
1114	16	-1.6346544462095451
1114	17	-1.4031779018277852
1114	18	-0.9798454942585124
1114	19	-0.7752916631742259
1114	20	-0.4856388607763549
1114	21	-0.39728280094750446
1114	22	-0.47277302859735926
1114	23	-0.9257050121424122
1114	24	-0.7759947196591064
1114	25	-0.6382615850570481
1115	1	0.8013336724362432
1115	2	0.9205190013665749
1115	3	1.688038310024929
1115	4	0.5311643003111612
1115	5	0.3241439662847405
1115	6	-0.18533589584397347
1115	7	-2.238919778187455
1115	8	-0.11198298989157252
1115	9	0.12792702033725334
1115	10	0.8869587925143563
1115	11	0.12665549557845068
1115	12	0.7681118719207838
1115	13	1.0850579793130706
1115	14	1.784198210124778
1115	15	0.8345767252592289
1115	16	0.549263079563648
1115	17	0.15323428355347346
1115	18	0.36064117798073525
1115	19	0.6523836409959216
1115	20	0.694858956628682
1115	21	0.7746518140798955
1115	22	0.3713358225218002
1115	23	0.3316647287324841
1115	24	0.2337749469146857
1115	25	0.41677510111365734
1116	1	0.6729732638858863
1116	2	0.9591190955214657
1116	3	1.7288829976649396
1116	4	1.1215800601115724
1116	5	0.34243992194551964
1116	6	-0.7555514514146893
1116	7	-4.366370932494586
1116	8	-0.5865169345494188
1116	9	-0.6681372369648706
1116	10	0.108062110450863
1116	11	-1.7733240359668514
1116	12	-0.04892413663958095
1116	13	-6.654942157143296
1116	14	-1.370047629661805
1116	15	-0.9530366756654907
1116	16	-0.5230153550999042
1116	17	-0.2328458026869491
1116	18	0.06816197051930217
1116	19	0.3502143259386443
1116	20	0.38651942590211874
1116	21	0.5974409366882156
1116	22	0.21412580935376013
1116	23	0.1668695611072124
1116	24	0.36312125269975226
1116	25	0.30824237091745843
1201	1	2.1838225126570414
1201	2	2.409414266035032
1201	3	3.0980162210337667
1201	4	2.631116595624281
1201	5	2.0663137728875363
1201	6	1.9655938421991692
1201	7	2.2641249109397066
1201	8	2.3730767075392585
1201	9	2.7761387278273126
1201	10	2.9777908220061775
1201	11	2.5122099383493897
1201	12	2.8556262336619476
1201	13	3.025408740282387
1201	14	3.687692960011283
1201	15	3.081128673357572
1201	16	2.609871478316375
1201	17	2.2924536738037915
1201	18	2.3007051262921046
1201	19	2.3643555364729707
1201	20	2.5559021207646246
1201	21	2.4699937063290065
1201	22	1.5253168094924803
1201	23	1.0051902187528228
1201	24	1.604082140417752
1201	25	1.721652734857269
1202	1	1.9833538340720196
1202	2	2.1806693093122362
1202	3	2.9984018477351846
1202	4	2.437898648389716
1202	5	1.8112925893344958
1202	6	1.6506785162035058
1202	7	1.9606923149863391
1202	8	2.2382335715880934
1202	9	2.5061942429243764
1202	10	-1.221176779731504
1202	11	1.1580645254782134
1202	12	2.135523890844926
1202	13	2.720952925577265
1202	14	3.3681936028387938
1202	15	2.7382106744838914
1202	16	2.283498831722124
1202	17	1.948685540561975
1202	18	2.234500541527968
1202	19	2.3362438612698853
1202	20	2.3888304561860387
1202	21	2.541899151087634
1202	22	2.275452870431863
1202	23	1.9831692360670203
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2211	20	0.5162210920860453
2211	21	0.4861805855965713
2211	22	0.43199636531628727
2211	23	0.22276458913892583
2211	24	0.46789945044533354
2211	25	0.5393358447164189
2212	1	0.6711599916698603
2212	2	0.6394856776881319
2212	3	1.3668940981322493
2212	4	0.829036111962111
2212	5	0.38010210696723234
2212	6	0.2692026182325016
2212	7	0.5794962117428497
2212	8	1.025406828131608
2212	9	0.8336575558976485
2212	10	1.5203602464694654
2212	11	0.661632484754243
2212	12	0.8709800898066469
2212	13	1.0893676367074647
2212	14	-4.843982271976127
2212	15	-1.3034351180740664
2212	16	-0.6183311718425664
2212	17	-0.31402508616092106
2212	18	0.1295034384410947
2212	19	0.41726057689102447
2212	20	0.7160256966010046
2212	21	0.738743485484612
2212	22	0.5836561789660806
2212	23	0.46082178520644845
2212	24	0.4822237277164696
2212	25	0.6266449031168726
2213	1	0.5756141334562948
2213	2	0.6507714749494937
2213	3	1.2045257626578127
2213	4	0.6607412931879111
2213	5	0.21113300358462794
2213	6	-0.07615031214040613
2213	7	0.1520550126102691
2213	8	-5.562491180318705
2213	9	-1.8935679323373655
2213	10	-0.05784292736802854
2213	11	-0.1888599325678122
2213	12	0.3490090629226401
2213	13	-8.572273699003105
2213	14	-2.6715069488069467
2213	15	-1.0987883252617188
2213	16	-0.49693526971174506
2213	17	-0.40412744262465594
2213	18	-0.06355450514309524
2213	19	0.28238500030396096
2213	20	0.4798868939860448
2213	21	0.45799075157795954
2213	22	0.05012560911043806
2213	23	0.042977271807050244
2213	24	0.14513125016915396
2213	25	0.2749886603306031
2214	1	-0.8589572692531746
2214	2	-0.48989400756435586
2214	3	-7.017602590237217
2214	4	-2.4254130393552593
2214	5	-1.4505638725345342
2214	6	-1.0059685962819849
2214	7	-0.6289702940647608
2214	8	-6.718265761875045
2214	9	-2.140258163994911
2214	10	-0.26624879443419047
2214	11	-0.531989310008651
2214	12	-0.16236129661291798
2214	13	-6.65671116300954
2214	14	-1.7479031712370237
2214	15	-1.038007899465203
2214	16	-0.8689306677825535
2214	17	-0.7955039415640321
2214	18	-0.7697354942430294
2214	19	-0.3617899779523128
2214	20	-0.22034523582299315
2214	21	-0.12440188428587007
2214	22	-0.37378317089294555
2214	23	-0.4407802007525703
2214	24	-0.19383271231751564
2214	25	-0.2603486592424211
2215	1	0.15081784124837938
2215	2	0.3728133391597943
2215	3	-2.7684323199783663
2215	4	-0.6864433129580725
2215	5	-0.296827231151223
2215	6	-0.7236164218240653
2215	7	-0.08644633489338105
2215	8	0.2872605556383192
2215	9	0.5006852940197604
2215	10	1.1503931741596531
2215	11	0.2506758937729465
2215	12	0.06055687624845163
2215	13	0.7020769194443304
2215	14	0.7871595108494027
2215	15	0.40361736617045096
2215	16	0.08120115288794594
2215	17	-0.0631520334228668
2215	18	0.13453817334211138
2215	19	0.053503080982114426
2215	20	0.4477840812251408
2215	21	0.42547921293670754
2215	22	0.28502141707738815
2215	23	0.16573113365782532
2215	24	0.11865424351853449
2215	25	0.1820527513670136
2216	1	0.05352548201238605
2216	2	-0.09872962101962912
2216	3	1.0749762501374818
2216	4	-0.4179331614208408
2216	5	-0.7054031262917029
2216	6	-0.9034567151480317
2216	7	-1.4048569793631565
2216	8	-6.879895837437921
2216	9	-2.4860642284660273
2216	10	-1.1339311912222065
2216	11	-1.6914935955974855
2216	12	-0.3830941765532465
2216	13	0.20745083773584128
2216	14	0.9530186587089382
2216	15	0.3609721582995462
2216	16	-0.2917001891880808
2216	17	-0.38154327597531434
2216	18	-0.19650662816182418
2216	19	-0.017661087722096624
2216	20	0.08050310307128683
2216	21	-0.09454913863099179
2216	22	-0.2985399575856107
2216	23	-0.4802195829953355
2216	24	-0.48249352437343873
2216	25	-0.4387986955825731
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	256075.0
3	8.0
4	1766.0
5	11392.0
6	15578.0
7	14233.0
8	13017.0
9	11043.0
10	10084.0
11	9484.0
12	8721.0
13	8543.0
14	8964.0
15	9417.0
16	9887.0
17	10624.0
18	12505.0
19	15927.0
20	21751.0
21	27348.0
22	34345.0
23	41571.0
24	47901.0
25	55386.0
26	63461.0
27	71111.0
28	78161.0
29	90870.0
30	105050.0
31	119141.0
32	149588.0
33	190032.0
34	240898.0
35	299066.0
36	376068.0
37	445337.0
38	607387.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	46.33801218483834	16.871246108738404	12.606523959540935	24.18421774688232
2	15.000165209233904	19.38493320518843	41.058603306770564	24.556298278807105
3	16.728720675924624	24.913758001720097	30.115030518388764	28.242490803966515
4	11.189093925836696	15.3751082265388	36.52546904871854	36.910328798905965
5	13.592050260908648	37.96544131466462	33.544794155462355	14.897714268964371
6	33.67000997766084	34.93476608790107	17.526849787665956	13.868374146772139
7	30.544497065622515	30.838193324233774	20.273457785866274	18.343851824277436
8	26.931939013440548	34.51860864430079	19.855932149838836	18.69352019241982
9	27.335982924812363	15.436374044496937	18.87456222715043	38.353080803540266
10	13.270100206658803	29.613283212271973	33.576922535010794	23.53969404605843
11	36.01607664121936	22.6758709353502	21.893954255734403	19.41409816769604
12	23.273074974350607	25.071060017854048	30.020416111839392	21.635448895955957
13	28.324616998733692	21.09641140141945	26.35300882279365	24.225962777053212
14	21.243064652930343	20.17976683849276	28.423513070492163	30.15365543808473
15	24.171966509892403	28.498183731472565	23.114113589732398	24.215736168902634
16	24.18813716803357	28.019812075370904	24.13307809513415	23.658972661461377
17	23.257823009683197	27.479157978901224	25.770669414585335	23.492349596830245
18	22.967721812734357	27.083486508344972	26.894564278361887	23.054227400558784
19	24.233513918395236	26.039230508315885	26.682426573834285	23.044828999454598
20	24.18354934703804	25.931198491170242	26.852058885568997	23.033193276222722
21	24.93628314009093	25.417239883853515	26.425106481973454	23.221370494082098
22	24.71967393399122	25.484605581849813	26.28756409494194	23.508156389217028
23	24.04157988394762	25.776334366875787	26.312970364251914	23.869115384924676
24	23.892249552384897	25.92003069688947	26.421497472848703	23.766222277876928
25	23.70580275037019	25.543968358451053	26.77346148941816	23.976767401760597
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	10514.0
1	10514.0
2	6371.5
3	2229.0
4	2229.0
5	2229.0
6	4201.0
7	6173.0
8	6173.0
9	6173.0
10	7324.0
11	8475.0
12	8475.0
13	8475.0
14	11742.0
15	15009.0
16	15009.0
17	15009.0
18	24236.5
19	33464.0
20	33464.0
21	33464.0
22	50107.5
23	66751.0
24	66751.0
25	66751.0
26	94437.5
27	122124.0
28	122124.0
29	122124.0
30	154356.0
31	186588.0
32	186588.0
33	186588.0
34	221890.5
35	257193.0
36	257193.0
37	257193.0
38	295897.5
39	334602.0
40	334602.0
41	334602.0
42	369138.0
43	403674.0
44	403674.0
45	403674.0
46	430640.5
47	457607.0
48	457607.0
49	457607.0
50	457861.0
51	458115.0
52	458115.0
53	458115.0
54	430847.0
55	403579.0
56	403579.0
57	403579.0
58	355572.0
59	307565.0
60	307565.0
61	307565.0
62	255893.5
63	204222.0
64	204222.0
65	204222.0
66	159171.0
67	114120.0
68	114120.0
69	114120.0
70	85907.5
71	57695.0
72	57695.0
73	57695.0
74	41131.5
75	24568.0
76	24568.0
77	24568.0
78	17966.0
79	11364.0
80	11364.0
81	11364.0
82	7620.0
83	3876.0
84	3876.0
85	3876.0
86	2705.0
87	1534.0
88	1534.0
89	1534.0
90	1057.5
91	581.0
92	581.0
93	581.0
94	336.0
95	91.0
96	91.0
97	91.0
98	59.0
99	27.0
100	27.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.3203274012383511
2	0.323764083236438
3	0.33527696793002915
4	0.3371098649956755
5	0.34303814144237543
6	0.28530188387451527
7	0.2936071987032253
8	0.29171702360427754
9	0.2920320527874355
10	0.40171948655970946
11	0.29117288228791377
12	0.29143063343777026
13	0.285330522891166
14	0.289798209488679
15	0.28283892844255304
16	0.2871061419235109
17	0.28157881170992116
18	0.2830680405757588
19	0.28378401599202685
20	0.4791021095499665
21	0.2843854353416921
22	0.2861324153573863
23	0.2824952602427443
24	0.2850727717413095
25	0.2788294661114516
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	3491740.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	62.587201051743136
#Duplication Level	Percentage of deduplicated	Percentage of total
1	84.74297261420466	53.03825464727589
2	8.038209690506731	10.061780919916295
3	2.790344297267201	5.239195186099415
4	1.3631756229961263	3.41269387141175
5	0.7617275281986478	2.383719697700805
6	0.47033416955728385	1.766213953895182
7	0.332884358458327	1.4584010188868286
8	0.24174474800697593	1.2104101717372464
9	0.16650428266170206	0.9378933313431793
>10	0.9793638846854654	11.151768322821342
>50	0.07228173045658763	3.114482747837825
>100	0.03764019338624792	4.192440093810925
>500	0.002124204302002412	0.9169654881061952
>1k	6.003186037601121E-4	0.6651157275488898
>5k	9.235670827078647E-5	0.45066482160821447
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
NNNNNNNNNNNNNNNNNNNNNNNNN	9176	0.262791616787046	No Hit
GTATCAACGCAGAGTACTTTTTTTT	6417	0.18377656984769772	No Hit
TATCAACGCAGAGTACTTTTTTTTT	4260	0.12200221093208544	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	2.863901665072428E-5	0.0	0.0	0.0	0.0
8	2.863901665072428E-5	0.0	0.0	0.0	0.0
9	2.863901665072428E-5	0.0	0.0	0.0	0.0
10	8.591704995217285E-5	0.0	0.0	0.0	0.0
11	8.591704995217285E-5	0.0	0.0	0.0	0.0
12	1.1455606660289713E-4	0.0	0.0	0.0	0.0
13	1.1455606660289713E-4	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACCGTCC	100	1.4439684E-7	12.350289	8
AGGACCG	325	0.0	12.282863	5
GGTATCA	2515	0.0	12.268692	1
GGACCGA	270	0.0	11.963588	6
TGGACCG	150	1.7462298E-10	11.405515	5
TAAGGCG	75	2.0659211E-4	11.405515	5
GTATCAA	6235	0.0	10.91781	1
ATAACGC	210	0.0	10.850377	3
TATAACG	225	0.0	10.548978	2
TAACGCA	220	0.0	10.36865	4
CATGGGT	2020	0.0	10.210382	4
ATGGGTA	1045	0.0	10.186744	5
CGTGCGA	75	0.0026741612	10.121782	11
GAACGCG	75	0.0026741612	10.121782	11
TATCACG	95	1.6555323E-4	9.993768	2
GTCTAGA	400	0.0	9.968783	1
CAGGACC	945	0.0	9.856619	4
CTCAACG	155	4.1080057E-8	9.800341	3
GTATTAG	340	0.0	9.773318	1
GTGGTAT	995	0.0	9.732624	1
>>END_MODULE