FastQCFastQC Report
Thu 26 May 2016
SRR1364313_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364313_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3491740
Sequences flagged as poor quality0
Sequence length25
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT124500.3565557573015173No Hit
TATCAACGCAGAGTACTTTTTTTTT97040.2779130175786284No Hit
GGTATCAACGCAGAGTACTTTTTTT90020.25780842788981995No Hit
GTACTTTTTTTTTTTTTTTTTTTTT44670.12793048737878537No Hit
GTGGTATCAACGCAGAGTACATGGG41970.1201979528830898No Hit
ACGCAGAGTACTTTTTTTTTTTTTT41510.11888055811715649No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAACGC2200.013.8196423
AGGACCG5200.012.9760565
TATAACG2200.012.9536872
CGCGCCA1550.012.8732510
TGCCGTA751.4843363E-512.66292315
ACCGAGT4150.012.5929548
TAACGCA2050.012.5142344
GGACCGA4350.012.4508396
GGTATCA37800.012.3408961
ACGGCCC3000.012.02977815
GTGGTAT13450.011.8670841
TGGTATC12850.011.3105342
CCGAGTG4400.011.0136329
CGTAGGG700.001490894910.8582893
CGAGTGG4650.010.82987610
GTATAAC3250.010.8162161
TTGCGCG3250.010.81125918
TGTACCG1502.1227606E-910.7707375
TCAATAC2700.010.556673
TATCACG909.528357E-510.5548552
Produced by FastQC (version 0.11.5)