##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1364313_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3491740
Sequences flagged as poor quality	0
Sequence length	25
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.846722264544326	31.0	30.0	34.0	26.0	34.0
2	29.89175282237509	31.0	30.0	34.0	26.0	34.0
3	29.953304656131326	31.0	30.0	34.0	26.0	34.0
4	33.359028449999144	35.0	33.0	37.0	28.0	37.0
5	32.899090138441004	35.0	33.0	37.0	26.0	37.0
6	32.80718209259567	35.0	33.0	37.0	26.0	37.0
7	32.55091931243449	35.0	33.0	37.0	25.0	37.0
8	32.456527977455366	35.0	33.0	37.0	24.0	37.0
9	33.25815696472246	37.0	33.0	39.0	18.0	39.0
10	33.384974826304365	37.0	33.0	39.0	19.0	39.0
11	33.333721010155394	37.0	33.0	39.0	19.0	39.0
12	33.21188089605755	37.0	33.0	39.0	18.0	39.0
13	33.31097733508222	37.0	33.0	39.0	19.0	39.0
14	34.27455308814517	38.0	33.0	41.0	19.0	41.0
15	34.303843642424695	38.0	33.0	41.0	19.0	41.0
16	34.246260030815584	38.0	33.0	41.0	19.0	41.0
17	34.17593320235756	38.0	33.0	41.0	18.0	41.0
18	34.11787418307205	38.0	33.0	41.0	18.0	41.0
19	33.999629124734376	39.0	33.0	41.0	18.0	41.0
20	33.84435668176897	39.0	33.0	41.0	17.0	41.0
21	33.776947309937164	39.0	33.0	41.0	16.0	41.0
22	33.74493805380698	39.0	33.0	41.0	11.0	41.0
23	33.44749551799389	38.0	33.0	41.0	8.0	41.0
24	33.35232319703071	38.0	33.0	41.0	2.0	41.0
25	33.20154822524014	38.0	33.0	41.0	2.0	41.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1101	1	0.264165810575399
1101	2	0.4526404340246266
1101	3	-1.3949030763472265
1101	4	-0.3667894907487437
1101	5	0.39584064329598334
1101	6	0.6312724819191189
1101	7	0.892952346363522
1101	8	0.9493388305907544
1101	9	1.7691096979086325
1101	10	1.2842564307511992
1101	11	1.3204899719414414
1101	12	1.4223876835148914
1101	13	1.17348264454764
1101	14	1.3654425967099755
1101	15	1.3851589481919575
1101	16	1.3127701444014335
1101	17	1.2852558268005936
1101	18	1.1868182839309611
1101	19	1.2884894762170305
1101	20	1.4779390483224475
1101	21	1.4004738388248015
1101	22	1.473457851250764
1101	23	1.8112121834378812
1101	24	1.7294086437375427
1101	25	1.9110793811003433
1102	1	0.59853554149187
1102	2	0.6239305822134433
1102	3	0.7013063527520984
1102	4	0.6583855593665362
1102	5	0.7996478417408923
1102	6	0.8453286387721661
1102	7	0.9560804421901494
1102	8	0.9913705233572827
1102	9	1.5724409658212153
1102	10	1.1859645378115147
1102	11	1.1803535207299376
1102	12	1.3319923465314645
1102	13	1.1601385047737338
1102	14	1.2994770727904523
1102	15	1.2501504175769966
1102	16	1.3083241813742816
1102	17	1.134326220659183
1102	18	1.2759354245411672
1102	19	1.1169197675642621
1102	20	1.4633020335829272
1102	21	1.5753157147418904
1102	22	1.521679309772587
1102	23	1.843971957211977
1102	24	1.850461098811131
1102	25	1.6540370366973676
1103	1	-2.7789450837586784
1103	2	-2.706481034108279
1103	3	-2.6391471761069063
1103	4	-2.7148665403622516
1103	5	-5.204627678651335
1103	6	-9.63963756036981
1103	7	-10.560904050920545
1103	8	-11.405616619291798
1103	9	-12.377575127896279
1103	10	-10.137709999213047
1103	11	-12.722138605097207
1103	12	-12.401642955853184
1103	13	-13.187452370037807
1103	14	-13.41939981462584
1103	15	-13.372015077695256
1103	16	-7.912159748304379
1103	17	-5.778655079231086
1103	18	-4.923497436500469
1103	19	-4.47814143616478
1103	20	-9.603629600426693
1103	21	-6.210283152843786
1103	22	-11.317174874259727
1103	23	-12.560755969834158
1103	24	-13.9414117865463
1103	25	-14.706897747994212
1104	1	0.24962178930342915
1104	2	0.4424337644066014
1104	3	0.39250492564762496
1104	4	0.6182197266090554
1104	5	0.6711539562958109
1104	6	0.6469560186655201
1104	7	0.4529068586897722
1104	8	0.7716311894696375
1104	9	1.1204884519812737
1104	10	0.9852466269367355
1104	11	0.813899374704171
1104	12	0.8817775709003044
1104	13	0.73482235295279
1104	14	0.7720218012201769
1104	15	0.7758942507503619
1104	16	0.2572625585272377
1104	17	0.18463005915708663
1104	18	0.5123402294966155
1104	19	0.6131250780209285
1104	20	0.8367274794849706
1104	21	0.7085952578973362
1104	22	0.31972019575607646
1104	23	0.8849139371028443
1104	24	1.0018531744222159
1104	25	1.0484465374808636
1105	1	0.2809081611451987
1105	2	0.36253097591717065
1105	3	-0.030499820469124472
1105	4	0.2883805039792904
1105	5	0.3099014312627304
1105	6	0.3271870564264532
1105	7	0.3783158426377895
1105	8	0.3725924822865707
1105	9	0.3607106750081286
1105	10	0.15108874784785087
1105	11	0.6657410345616412
1105	12	0.841543097364422
1105	13	0.7442670126360582
1105	14	0.5106090137807868
1105	15	0.4175417169065767
1105	16	0.5696755829304934
1105	17	0.6220477604788073
1105	18	0.6822154633928292
1105	19	0.678071282394825
1105	20	0.8805658958743408
1105	21	0.8359993448602623
1105	22	0.669989204060272
1105	23	1.1301956807063291
1105	24	1.0211145351097883
1105	25	1.1331174694971793
1106	1	-0.17821313648173387
1106	2	-0.036327749378241236
1106	3	-0.16944188898294144
1106	4	-1.462002740636379
1106	5	-0.45160059142292397
1106	6	-0.1077625861885565
1106	7	0.18998478183790724
1106	8	0.38707566381687286
1106	9	0.858929915313766
1106	10	0.312572398764118
1106	11	0.4507017815232075
1106	12	0.5836901475691505
1106	13	0.32124338311012934
1106	14	-0.23659385505332153
1106	15	0.3622659054660957
1106	16	0.08766617427001222
1106	17	0.07688120974648172
1106	18	0.0683075655434493
1106	19	-1.388292205301326
1106	20	-0.10418605217384425
1106	21	-0.36562886095149594
1106	22	0.24300730896714384
1106	23	0.10261322783333071
1106	24	0.2165588529426401
1106	25	0.5668982969748981
1107	1	0.11666407619833663
1107	2	0.009825225786293856
1107	3	-0.11069883764715271
1107	4	0.029181038954646965
1107	5	0.2127989500460501
1107	6	0.05740614738324723
1107	7	-0.009590407324076011
1107	8	-0.1730299085797995
1107	9	0.684433000344896
1107	10	0.536600482771469
1107	11	0.4592263916857604
1107	12	0.3221542066450951
1107	13	0.2678958027058229
1107	14	0.4651379389367136
1107	15	0.3498644056458531
1107	16	0.14609230969646347
1107	17	-0.05063097242034331
1107	18	0.15145224490103715
1107	19	0.3081353354432963
1107	20	0.2790703688491831
1107	21	0.24191311669278548
1107	22	0.20836245919739582
1107	23	0.37261737161406927
1107	24	0.5497031959263836
1107	25	0.23860060081141654
1108	1	-0.11079584641083784
1108	2	-0.11717864375488674
1108	3	0.05635247983948943
1108	4	-0.2873727660941867
1108	5	0.0637548188501782
1108	6	0.05445331893290728
1108	7	-0.4161432910535794
1108	8	0.017720045354238323
1108	9	0.3622015649293857
1108	10	0.07269086419766779
1108	11	0.24823735538578262
1108	12	0.2778203205201635
1108	13	-0.2099671971099113
1108	14	-0.016601511956970683
1108	15	-0.2399550157656094
1108	16	-0.7235202755255727
1108	17	-0.8121290376497541
1108	18	-0.729669922042028
1108	19	-0.6373686262298222
1108	20	0.05212408176607397
1108	21	-0.17204487114580047
1108	22	-0.17389293052068666
1108	23	-0.5386204209048202
1108	24	-0.19875589749539557
1108	25	-0.29284172459856705
1109	1	0.24908281831351786
1109	2	0.29437806637692887
1109	3	0.19554753869645936
1109	4	0.2796293069502269
1109	5	0.2560931979125698
1109	6	0.49112782499625496
1109	7	0.6061166211597566
1109	8	0.3146527048911949
1109	9	1.2779617586503917
1109	10	0.6158732880061137
1109	11	0.5360715432634606
1109	12	0.5719564882440764
1109	13	0.7238528415905634
1109	14	0.40558730590209535
1109	15	0.10018264968848456
1109	16	0.554612419814795
1109	17	0.29468987092037935
1109	18	0.3170462772216851
1109	19	0.5892812684097777
1109	20	0.8226991158613046
1109	21	0.9058003378941493
1109	22	0.5731450894144317
1109	23	0.9820021688565035
1109	24	0.6648048125052313
1109	25	0.7723283620675332
1110	1	0.042194431018604206
1110	2	0.039013491742100115
1110	3	-0.060036815194102644
1110	4	0.1191859001740454
1110	5	0.026384888914591897
1110	6	0.20058230041092884
1110	7	0.1647024578564995
1110	8	0.006896081512380192
1110	9	-3.45245993273069
1110	10	-1.4661242442073892
1110	11	-0.42617719591918757
1110	12	0.0686180302197883
1110	13	-0.3120012928094624
1110	14	-0.022781907796861844
1110	15	-0.042733312341361795
1110	16	-0.3479294906227679
1110	17	-0.10710366398180327
1110	18	-0.015666912420840617
1110	19	-0.272778900564262
1110	20	0.15885937651151494
1110	21	-0.09702926273806867
1110	22	0.19363037536675165
1110	23	0.38438950710973785
1110	24	0.47545441045715364
1110	25	0.5360452358441208
1111	1	0.05231062214165405
1111	2	0.049390257486443545
1111	3	0.01292542309100142
1111	4	-0.10269382196077714
1111	5	0.05082055329345536
1111	6	0.12175679706596299
1111	7	0.12780187790912834
1111	8	0.289369853609287
1111	9	0.8788780304086146
1111	10	0.3637087492575972
1111	11	0.5221518585191731
1111	12	0.4962492145460047
1111	13	0.2751476828323618
1111	14	0.07595183589642573
1111	15	0.13140684102029354
1111	16	0.19584045216988244
1111	17	0.1540977959530494
1111	18	0.1840228050120487
1111	19	0.33201966062596
1111	20	0.3864669711686588
1111	21	0.34012324975373076
1111	22	0.45709759580917364
1111	23	0.76020155025995
1111	24	0.6460434384123488
1111	25	0.899175639342694
1112	1	-0.10091929659178334
1112	2	-0.1711894362772135
1112	3	-0.11330291963373185
1112	4	0.007027274591912658
1112	5	-0.09109244768657732
1112	6	-0.1786287599124634
1112	7	3.612392318785851E-5
1112	8	-0.4345974560911934
1112	9	0.21182361251069892
1112	10	0.05956344176892259
1112	11	0.14642602109596936
1112	12	-0.3222283957264338
1112	13	-0.027665916568572868
1112	14	-1.0326714492147246
1112	15	-0.325705781306624
1112	16	-0.4236270765277368
1112	17	-0.34946013681675936
1112	18	-0.4207002438904581
1112	19	-0.12417776561181881
1112	20	-0.18271579703218066
1112	21	-0.45535179420625127
1112	22	-0.163269041358177
1112	23	0.08485947159822871
1112	24	-0.026611686698593928
1112	25	-0.007145831752893628
1113	1	-2.015486298842845
1113	2	-2.0482045243094404
1113	3	-2.0992580698303698
1113	4	-2.097825569644005
1113	5	-1.9351212665832485
1113	6	-1.946939963587635
1113	7	-2.3481651695699597
1113	8	-2.364000087976269
1113	9	-11.013284093549206
1113	10	-4.41048014066422
1113	11	-3.0965883914136505
1113	12	-12.599103736293916
1113	13	-5.015715748096088
1113	14	-4.022253196634576
1113	15	-3.4315507356017036
1113	16	-3.2450460923872093
1113	17	-3.2709313985338078
1113	18	-3.338371435810309
1113	19	-3.995171008344851
1113	20	-14.171574725053311
1113	21	-16.912176715480314
1113	22	-11.324639199177401
1113	23	-17.73758196099951
1113	24	-18.959289378737836
1113	25	-15.210400534390292
1114	1	-0.1778665738547005
1114	2	-0.08677256603537842
1114	3	-0.06658855821348908
1114	4	-0.5895372813557174
1114	5	-0.16775284553061454
1114	6	-0.051141006736045824
1114	7	-0.23259380873544444
1114	8	-0.04431303249973695
1114	9	0.6999923407798576
1114	10	-0.024979903585034435
1114	11	-0.18520859056016548
1114	12	-0.3046134565404799
1114	13	-0.2605900961283325
1114	14	-0.27826078316847713
1114	15	-0.028174728015166295
1114	16	-0.270091137432253
1114	17	-0.24240603106107272
1114	18	-0.18750372599523502
1114	19	-0.503705858984965
1114	20	-0.7351688454895395
1114	21	-0.9301852503309576
1114	22	-1.1028373973756018
1114	23	-0.6361500027254436
1114	24	-0.2870371264855791
1114	25	-0.6225200222992555
1115	1	-0.1994389709941089
1115	2	-0.19582151188844676
1115	3	-0.20731949533617922
1115	4	-0.09549901628652435
1115	5	-0.4226975840836005
1115	6	-0.31726156027927743
1115	7	-0.5088889698759615
1115	8	-0.22002678749184668
1115	9	0.24984838238847829
1115	10	-0.25876461613263757
1115	11	-0.03445164805064849
1115	12	-0.0683303693295585
1115	13	-0.31311447617848387
1115	14	-0.4952469445876275
1115	15	-0.44623546916869117
1115	16	-0.2836083660010331
1115	17	-0.26642200579869524
1115	18	-0.3250666497437251
1115	19	-0.37443586734384127
1115	20	-0.7389944236450106
1115	21	-0.2227885753054153
1115	22	-0.6596907455691507
1115	23	-0.34573113024471525
1115	24	0.07235824827061776
1115	25	0.11241357108794858
1116	1	-0.5153744589734401
1116	2	-0.39475749338496513
1116	3	-0.4330852998260504
1116	4	-0.23142422455305223
1116	5	-1.0979796507607311
1116	6	-1.002590795373127
1116	7	-2.0628807733969907
1116	8	-1.273348931403273
1116	9	-0.2506277366105678
1116	10	-1.1757310319470164
1116	11	-0.8389648285983355
1116	12	-1.7866116559311358
1116	13	-1.4712255562078695
1116	14	-0.9725499300254583
1116	15	-2.159152542361614
1116	16	-2.294282323986117
1116	17	-3.056062579734167
1116	18	-2.2309802137337975
1116	19	-1.705273641194708
1116	20	-2.1233081959584688
1116	21	-1.3193719353614526
1116	22	-1.189099821281964
1116	23	-1.3142393892661879
1116	24	-1.7925776968375366
1116	25	-1.4105406637216156
1201	1	0.6724087364206106
1201	2	0.7647350254722127
1201	3	0.30630251467758285
1201	4	0.7237960519776152
1201	5	0.9427706849929152
1201	6	0.9983972028021668
1201	7	1.1849844688428348
1201	8	1.169449961263254
1201	9	1.8257971665846853
1201	10	1.5122884136832795
1201	11	1.4415349361833592
1201	12	1.5567637248366992
1201	13	1.4773220817838464
1201	14	1.5399487550410598
1201	15	1.4717786519382088
1201	16	1.3907127433716227
1201	17	1.4216448729898303
1201	18	1.2092416907501828
1201	19	1.3398208845417798
1201	20	1.5423426520219579
1201	21	1.5690814289357249
1201	22	1.5948089202707223
1201	23	1.8837869972115513
1201	24	1.826724607276148
1201	25	1.8037585639793292
1202	1	0.7391741788093213
1202	2	0.8191059995107786
1202	3	0.7641643553649082
1202	4	0.8590958947566847
1202	5	0.9714695347443936
1202	6	0.9692488013545955
1202	7	1.0490348004831986
1202	8	1.1068092254923272
1202	9	1.6694141171268413
1202	10	1.3033328412928071
1202	11	1.4828980202057238
1202	12	1.4662851983470944
1202	13	1.4790859788386044
1202	14	1.5025203303550185
1202	15	1.442842216907593
1202	16	1.364966135907565
1202	17	1.3370743911894323
1202	18	1.409506081596291
1202	19	1.3693918925061368
1202	20	1.63604674683409
1202	21	1.6278896666913312
1202	22	1.6910579384033966
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2211	5	-0.8402945944048845
2211	6	-0.1736417540778703
2211	7	0.10182834656691142
2211	8	-0.08957060582577014
2211	9	0.5200635821224964
2211	10	-0.1440937292514306
2211	11	0.22748552164495095
2211	12	0.20017657974099734
2211	13	0.2941816578121248
2211	14	0.07636641126311616
2211	15	0.0801447126270105
2211	16	0.13355696054347277
2211	17	-0.04234913868144474
2211	18	-0.10332270249880793
2211	19	-0.31872129356030143
2211	20	0.10254954352469525
2211	21	0.34744282832402007
2211	22	0.23278308513975077
2211	23	0.6631151163929729
2211	24	0.5909773042916839
2211	25	0.7293933930371423
2212	1	-0.14620529759404732
2212	2	-0.09210241201038372
2212	3	-0.028857591034032737
2212	4	-0.010827228951598045
2212	5	-0.2944303867555078
2212	6	-0.19905243533425931
2212	7	-0.1495258708704199
2212	8	-0.2548096060764351
2212	9	0.2933897965082224
2212	10	-0.16721050046703567
2212	11	-0.061739231502876635
2212	12	-0.1594636032532719
2212	13	-0.3023094012815619
2212	14	-0.2612797855215092
2212	15	-0.21389980793379948
2212	16	-0.06539745311317802
2212	17	-0.4510807411509745
2212	18	-0.4716336207182579
2212	19	-0.23147535584915602
2212	20	-0.05695377297685411
2212	21	0.11647429162515266
2212	22	-0.2247779435409072
2212	23	-0.011524565633827422
2212	24	0.17878751188848696
2212	25	0.19420748564573387
2213	1	-0.17773034463450088
2213	2	-0.18986345011862937
2213	3	-0.23136839703913026
2213	4	-0.233140759921028
2213	5	-0.20247704231588415
2213	6	0.013287099371446232
2213	7	-0.15412984164552057
2213	8	-0.056152783423456754
2213	9	0.5044405780525238
2213	10	-0.05876700088358433
2213	11	0.20511625892498841
2213	12	0.03686099501030782
2213	13	-0.27149306016107033
2213	14	-0.14788316752368758
2213	15	-0.08243121906555473
2213	16	-0.30531579998515923
2213	17	-0.2829215014238713
2213	18	-0.5031833403771344
2213	19	-0.45955108658591826
2213	20	0.04161767038095121
2213	21	-0.2000721531949594
2213	22	0.031051293535995228
2213	23	-0.4190021261882535
2213	24	-0.2456642685478414
2213	25	0.16579383631994915
2214	1	-0.8936146745933478
2214	2	-0.7385867689648933
2214	3	-0.7385644867111587
2214	4	-0.6391993544960499
2214	5	-0.9663680993821657
2214	6	-0.7916825381282386
2214	7	-0.7594694831725519
2214	8	-0.7836865665424604
2214	9	-10.685156805616923
2214	10	-3.8178052396617908
2214	11	-1.7084326050885288
2214	12	-1.1240274606803808
2214	13	-0.9043153887005033
2214	14	-1.0507418602242282
2214	15	-0.9398494685746002
2214	16	-1.1894621014535005
2214	17	-1.0423595229616325
2214	18	-1.3770136724428
2214	19	-1.520415798001146
2214	20	-1.1806681031249013
2214	21	-1.6652402193745814
2214	22	-1.478926877472638
2214	23	-1.803401612472328
2214	24	-2.201722600755179
2214	25	-9.827261619025727
2215	1	-0.5485900868226032
2215	2	-0.2995465512781905
2215	3	-0.12249737048140474
2215	4	-0.2888027598730645
2215	5	-0.2767157664137372
2215	6	-0.19062601365247644
2215	7	-0.30464412053637346
2215	8	-0.3810293498145576
2215	9	0.39202705451033637
2215	10	0.32587699535166337
2215	11	0.36590301267114
2215	12	0.3379961070665871
2215	13	0.17266916241771213
2215	14	0.01997708419656874
2215	15	-0.4547876038051015
2215	16	-0.1716658364633119
2215	17	-0.11486606501736674
2215	18	-0.12264829700015412
2215	19	-0.11974876330717166
2215	20	0.07888533694369926
2215	21	-0.027202552635039012
2215	22	-0.10639908517669028
2215	23	0.3160048097286179
2215	24	0.46716571903895954
2215	25	0.5770010591669674
2216	1	-0.5778099935200203
2216	2	-0.599301426629129
2216	3	-0.6296787631345175
2216	4	-0.4596620295105893
2216	5	-1.2573708164232116
2216	6	-1.767534181900949
2216	7	-1.02183558154616
2216	8	-1.914959381547014
2216	9	-0.634651407421245
2216	10	-1.082190734677674
2216	11	-0.6090524145198657
2216	12	-1.7388478697320195
2216	13	-1.388469168711925
2216	14	-1.5743884751710269
2216	15	-1.2444295393568865
2216	16	-2.136944247784786
2216	17	-2.5116014241035103
2216	18	-2.1198325421479183
2216	19	-3.47374974826408
2216	20	-2.3087825154415604
2216	21	-2.4452067475772488
2216	22	-2.3008351932181235
2216	23	-1.8898823511970768
2216	24	-1.944576403885808
2216	25	-2.0823629872638882
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	107049.0
3	4.0
4	1666.0
5	16444.0
6	20773.0
7	16851.0
8	13150.0
9	11254.0
10	9986.0
11	9029.0
12	8322.0
13	7848.0
14	7624.0
15	7848.0
16	8338.0
17	9037.0
18	10256.0
19	12180.0
20	15368.0
21	20140.0
22	25534.0
23	30526.0
24	36564.0
25	41675.0
26	46979.0
27	52086.0
28	58837.0
29	69067.0
30	84699.0
31	104470.0
32	130056.0
33	159711.0
34	199425.0
35	248794.0
36	321008.0
37	462183.0
38	1106959.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.02092050448931	17.963450708704006	12.510363138110051	25.505265648696636
2	16.8958717875651	19.810350530757898	37.796176939346246	25.49760074233075
3	19.598949862432207	23.92104436393046	28.38467316945482	28.09533260418251
4	12.899646725082805	15.709406678571378	34.89654500854189	36.49440158780393
5	13.921295410350403	37.767236825369174	33.53593695749077	14.775530806789652
6	34.04429000570553	35.31984171174949	16.85607753099659	13.779790751548399
7	30.393123475752105	30.870227030941265	20.050036292639557	18.686613200667075
8	27.458231900681273	35.36834590821302	18.477739626941737	18.69568256416397
9	26.81247752890267	15.04737730959908	18.324268498637053	39.81587666286121
10	15.287451030501042	29.727472597994865	31.633479809697235	23.351596561806858
11	36.61612370514938	22.08543292332834	21.357829727787703	19.94061364373458
12	23.77101227534183	25.145103596736163	29.094384573149828	21.989499554772184
13	29.52071560580506	20.781992889880208	25.15007977619705	24.54721172811768
14	22.575655206841926	20.09135820360753	26.654668109777628	30.678318479772916
15	24.616249126813713	28.45987266874656	22.59853364849666	24.325344555943065
16	24.487866995357486	27.67578987044552	24.048734228153705	23.78760890604329
17	23.482442573179814	27.191384544881707	25.86936472475225	23.45680815718623
18	23.088781324463135	26.686327297370767	26.997935691062292	23.22695568710381
19	24.388185073889833	25.812647817619734	26.53615035012206	23.263016758368373
20	24.14416628428476	25.517246712804553	26.943917893582686	23.394669109328
21	24.801282014561576	25.087358007435455	26.466239710372403	23.645120267630563
22	24.680974527798767	25.13454434220634	26.570266373452135	23.614214756542765
23	23.791542602612765	25.28351906185235	26.861189938009026	24.06374839752586
24	23.9868929685032	25.533650994497876	26.626715099635952	23.852740937362974
25	23.768255630139258	25.126272110161917	26.909706195518364	24.195766064180464
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	717.0
1	717.0
2	978.0
3	1239.0
4	1239.0
5	1239.0
6	3566.5
7	5894.0
8	5894.0
9	5894.0
10	6578.0
11	7262.0
12	7262.0
13	7262.0
14	8933.5
15	10605.0
16	10605.0
17	10605.0
18	19057.5
19	27510.0
20	27510.0
21	27510.0
22	43338.0
23	59166.0
24	59166.0
25	59166.0
26	87847.5
27	116529.0
28	116529.0
29	116529.0
30	145729.0
31	174929.0
32	174929.0
33	174929.0
34	210175.0
35	245421.0
36	245421.0
37	245421.0
38	281797.0
39	318173.0
40	318173.0
41	318173.0
42	353248.5
43	388324.0
44	388324.0
45	388324.0
46	421052.5
47	453781.0
48	453781.0
49	453781.0
50	462279.0
51	470777.0
52	470777.0
53	470777.0
54	446280.0
55	421783.0
56	421783.0
57	421783.0
58	374617.0
59	327451.0
60	327451.0
61	327451.0
62	275790.0
63	224129.0
64	224129.0
65	224129.0
66	175081.5
67	126034.0
68	126034.0
69	126034.0
70	95474.0
71	64914.0
72	64914.0
73	64914.0
74	45846.0
75	26778.0
76	26778.0
77	26778.0
78	20028.5
79	13279.0
80	13279.0
81	13279.0
82	8933.0
83	4587.0
84	4587.0
85	4587.0
86	3112.5
87	1638.0
88	1638.0
89	1638.0
90	1194.5
91	751.0
92	751.0
93	751.0
94	409.5
95	68.0
96	68.0
97	68.0
98	34.5
99	1.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.02869629468402573
2	0.00100236558277535
3	2.0047311655506996E-4
4	0.003579877081340535
5	0.02909724091713587
6	0.01111193846048102
7	0.019302697222588164
8	0.02629061728536489
9	0.0327916740650793
10	0.03728799967924301
11	0.03940728691139661
12	0.007703895479044831
13	0.002777984615120255
14	0.005985554480001374
15	0.006214666613207169
16	0.0089640122116767
17	0.009737265661246256
18	0.014290869308711417
19	0.015150039808233144
20	0.013345781759237514
21	0.010310045994260742
22	0.009336319428136116
23	0.007560700395791211
24	0.005126383980479646
25	0.10418874257533492
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	3491740.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	47.56431078304188
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.58081547667548	35.949493965683345
2	11.900365362405072	11.320653530583634
3	4.698910853954687	6.705013685979284
4	2.3236982212202912	4.421004174404941
5	1.331258574449023	3.1660198283841314
6	0.8308831948978915	2.371223190391804
7	0.5789428696234249	1.9275913003476273
8	0.4233641189571754	1.610961802277424
9	0.30706880186398106	1.3144964331271234
>10	1.7895989735763425	15.747272029717818
>50	0.14905543347658193	4.917203060546016
>100	0.07951534127897401	6.814152224344718
>500	0.0046676757325096645	1.4337885212105512
>1k	0.001675575899400351	1.4143195297841957
>5k	1.1968399281431078E-4	0.5324369805015832
>10k+	5.984199640715539E-5	0.354369742715958
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	12450	0.3565557573015173	No Hit
TATCAACGCAGAGTACTTTTTTTTT	9704	0.2779130175786284	No Hit
GGTATCAACGCAGAGTACTTTTTTT	9002	0.25780842788981995	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	4467	0.12793048737878537	No Hit
GTGGTATCAACGCAGAGTACATGGG	4197	0.1201979528830898	No Hit
ACGCAGAGTACTTTTTTTTTTTTTT	4151	0.11888055811715649	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	2.863901665072428E-5	0.0	0.0	0.0	0.0
3	2.863901665072428E-5	0.0	0.0	0.0	0.0
4	2.863901665072428E-5	0.0	0.0	2.863901665072428E-5	0.0
5	5.727803330144856E-5	0.0	0.0	2.863901665072428E-5	0.0
6	1.1455606660289713E-4	0.0	0.0	2.863901665072428E-5	0.0
7	2.0047311655506996E-4	0.0	0.0	2.863901665072428E-5	0.0
8	2.0047311655506996E-4	0.0	0.0	2.863901665072428E-5	0.0
9	2.0047311655506996E-4	0.0	0.0	2.863901665072428E-5	0.0
10	2.2911213320579425E-4	0.0	0.0	2.863901665072428E-5	0.0
11	2.2911213320579425E-4	0.0	0.0	2.863901665072428E-5	0.0
12	2.2911213320579425E-4	0.0	0.0	2.863901665072428E-5	0.0
13	2.2911213320579425E-4	0.0	0.0	2.863901665072428E-5	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATAACGC	220	0.0	13.819642	3
AGGACCG	520	0.0	12.976056	5
TATAACG	220	0.0	12.953687	2
CGCGCCA	155	0.0	12.87325	10
TGCCGTA	75	1.4843363E-5	12.662923	15
ACCGAGT	415	0.0	12.592954	8
TAACGCA	205	0.0	12.514234	4
GGACCGA	435	0.0	12.450839	6
GGTATCA	3780	0.0	12.340896	1
ACGGCCC	300	0.0	12.029778	15
GTGGTAT	1345	0.0	11.867084	1
TGGTATC	1285	0.0	11.310534	2
CCGAGTG	440	0.0	11.013632	9
CGTAGGG	70	0.0014908949	10.858289	3
CGAGTGG	465	0.0	10.829876	10
GTATAAC	325	0.0	10.816216	1
TTGCGCG	325	0.0	10.811259	18
TGTACCG	150	2.1227606E-9	10.770737	5
TCAATAC	270	0.0	10.55667	3
TATCACG	90	9.528357E-5	10.554855	2
>>END_MODULE