FastQCFastQC Report
Thu 26 May 2016
SRR1364312_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364312_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1920134
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT138480.7211996662732914No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA118960.6195400945975645No Hit
TATCAACGCAGAGTACTTTTTTTTT74750.3892957470676526No Hit
GGTATCAACGCAGAGTACTTTTTTT64790.3374243672577018No Hit
NNNNNNNNNNNNNNNNNNNNNNNNN55380.2884173708709913No Hit
GAAAAAAAAAAAAAAAAAAAAAAAA50380.26237752156880717No Hit
GTACTTTTTTTTTTTTTTTTTTTTT34330.17878960530879615No Hit
ACGCAGAGTACTTTTTTTTTTTTTT31410.16358233331632063No Hit
GAACAAAAAAAAAAAAAAAAAAAAA29900.15571829882706104No Hit
GAGTACTTTTTTTTTTTTTTTTTTT23990.12493919695187938No Hit
GTGGTATCAACGCAGAGTACATGGG22800.11874171281795957No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATACCGT508.724418E-515.1982016
CGCATAT1850.014.889358
TATAACG1600.014.8350112
ATAACGC1700.013.9619973
CGCGCCA1101.8735591E-1013.81582410
CGCCAGT1001.0228177E-813.28591812
GTTATCA4250.012.9567351
GGTATCA30600.012.7519571
ACAACCG1405.2750693E-1112.2179525
CTACACG1650.012.0945394
TATCACG550.003082456312.0837912
TAACGCA2150.011.9338134
TCGCACG1459.640644E-1111.80312818
ACGTCTT752.057485E-411.40998916
GTATCAA74300.011.1042041
TCGCGGT600.005838309411.09304517
ACGCATA4800.011.0820217
TTTACCG600.00590346411.0765183
TAGCACC951.3560188E-511.0033034
ACAGCGT951.3631134E-510.9981228
Produced by FastQC (version 0.11.5)