FastQCFastQC Report
Thu 26 May 2016
SRR1364312_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364312_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1920134
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT346541.8047698754357768No Hit
GGTATCAACGCAGAGTACTTTTTTT265371.38203896186412No Hit
TATCAACGCAGAGTACTTTTTTTTT257661.341885514240152No Hit
ACGCAGAGTACTTTTTTTTTTTTTT121500.6327683380430741No Hit
GTACTTTTTTTTTTTTTTTTTTTTT108400.5645439328713517No Hit
GAGTACTTTTTTTTTTTTTTTTTTT85580.4456980606561834No Hit
GTGGTATCAACGCAGAGTACTTTTT52540.2736267364673507No Hit
ATCAACGCAGAGTACTTTTTTTTTT41390.21555787252348016No Hit
GTGGTATCAACGCAGAGTACATGGG40390.21034990266304332No Hit
GCAGAGTACTTTTTTTTTTTTTTTT32380.16863406408094433No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA22320.1162418872849499No Hit
TGCATGCACTGCCTCAGTGACCAGT20090.10462811449617579No Hit
GTATAACGCAGAGTACTTTTTTTTT19750.10285740474362728No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATAACG2450.015.8966282
ATAACGC2500.015.2007083
GCGAGAC653.3824454E-614.61149713
TAACGCA2750.014.5097684
GACCGGT400.005273509314.2525217
GTTTCGA400.005290855714.24546717
GTATAAC3750.014.1895471
GCCCCCG2350.014.14406918
ATTCGCC551.9601878E-413.81846611
TATCACG1101.8735591E-1013.8170252
CGTAAGA500.001500717113.2988882
GGTATCA61900.013.0632331
ACCGAGT1102.741217E-912.9568388
ACGGCCC2450.012.79251515
CCGTAAG604.085801E-412.6692391
CGCGCCA1207.403287E-1012.66791710
GGCCCCC2550.012.66263817
TCCCGAT1301.9826984E-1012.41912617
GTGGTAT18650.012.1767331
TGACCGA1800.012.14673419
Produced by FastQC (version 0.11.5)