##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1364312_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1920134
Sequences flagged as poor quality	0
Sequence length	25
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.436877842900547	31.0	30.0	34.0	26.0	34.0
2	29.425085957542546	31.0	30.0	34.0	25.0	34.0
3	29.472609724113006	31.0	30.0	34.0	25.0	34.0
4	32.81543058974009	35.0	33.0	37.0	26.0	37.0
5	32.38033126854688	35.0	32.0	37.0	25.0	37.0
6	32.21089882268633	35.0	32.0	37.0	22.0	37.0
7	31.940428636751392	35.0	32.0	37.0	19.0	37.0
8	31.80968255340513	35.0	32.0	37.0	17.0	37.0
9	32.485397373308324	37.0	32.0	39.0	11.0	39.0
10	32.64096620340039	37.0	32.0	39.0	17.0	39.0
11	32.55710226473777	37.0	32.0	39.0	13.0	39.0
12	32.51247881658259	37.0	32.0	39.0	15.0	39.0
13	32.51623220046101	37.0	32.0	39.0	13.0	39.0
14	33.393060067682775	38.0	32.0	40.0	11.0	41.0
15	33.424837537380206	38.0	32.0	41.0	11.0	41.0
16	33.38992278663885	38.0	32.0	40.0	11.0	41.0
17	33.42083885812136	38.0	32.0	41.0	11.0	41.0
18	33.39259343358328	38.0	32.0	41.0	10.0	41.0
19	33.33789516773309	38.0	32.0	41.0	10.0	41.0
20	33.26598768627606	38.0	32.0	41.0	9.0	41.0
21	33.21575733776913	38.0	32.0	41.0	2.0	41.0
22	33.15496262240031	38.0	32.0	41.0	2.0	41.0
23	32.9002574820299	38.0	32.0	41.0	2.0	41.0
24	32.809368512822545	38.0	32.0	41.0	2.0	41.0
25	32.63404949862874	38.0	32.0	41.0	2.0	41.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1101	1	0.254735807994372
1101	2	0.47662726455466853
1101	3	-1.4618591829212164
1101	4	-0.30239522594329316
1101	5	0.40063876137267584
1101	6	0.7471722363841238
1101	7	0.8705461254328135
1101	8	0.9426447344209272
1101	9	1.6742442454942221
1101	10	1.3249308526395112
1101	11	1.2814910977061018
1101	12	1.352427313712397
1101	13	1.1798485259760483
1101	14	1.3877106136928816
1101	15	1.4701735963893086
1101	16	1.3505162540555276
1101	17	1.3357994819701844
1101	18	1.2819484493947755
1101	19	1.38572691218981
1101	20	1.6120610527550596
1101	21	1.4348651653821491
1101	22	1.5454008640232502
1101	23	1.70649399615737
1101	24	1.7356938873205934
1101	25	1.910697497794402
1102	1	0.664138858654848
1102	2	0.7039420739763571
1102	3	0.8482723239853485
1102	4	0.7791262540705475
1102	5	0.9355694615334968
1102	6	0.9609145838225714
1102	7	1.1285638938709859
1102	8	1.0570058919718512
1102	9	1.6975977911055153
1102	10	1.3247633210333802
1102	11	1.1791811671170294
1102	12	1.3549755955406724
1102	13	1.099866895606496
1102	14	1.238722294428797
1102	15	1.2774720177371819
1102	16	1.268570600828177
1102	17	1.1255649701470745
1102	18	1.3282713588195065
1102	19	1.3045918957663076
1102	20	1.4841596185043713
1102	21	1.586867852613132
1102	22	1.4910353566552814
1102	23	1.749878557333247
1102	24	1.8174721290361546
1102	25	1.6235341537578591
1103	1	-2.3984330951431865
1103	2	-2.286418926214342
1103	3	-2.258891240441269
1103	4	-2.1510263120595248
1103	5	-4.990606664027769
1103	6	-9.577581380343872
1103	7	-10.031248822391714
1103	8	-10.50605308429856
1103	9	-11.59262779308067
1103	10	-9.269129282324858
1103	11	-12.184246751951846
1103	12	-10.882341433936869
1103	13	-12.193337969096287
1103	14	-12.145042424597438
1103	15	-12.824590812977398
1103	16	-6.595231333521063
1103	17	-4.743898881356333
1103	18	-3.829255530150288
1103	19	-3.247713418846356
1103	20	-8.26938774788228
1103	21	-4.924182773966802
1103	22	-10.511418697253003
1103	23	-11.757020981199318
1103	24	-12.798198115977566
1103	25	-13.745967901881489
1104	1	0.2664131069911484
1104	2	0.41390636055162133
1104	3	0.5100752607286196
1104	4	0.5435408282703236
1104	5	0.648975599283311
1104	6	0.721374277933819
1104	7	0.5360284703093079
1104	8	0.8378492155359432
1104	9	1.182536276998654
1104	10	1.012066859888236
1104	11	0.8670458349339611
1104	12	0.8327701337170694
1104	13	0.7694366699495347
1104	14	0.9239354901431867
1104	15	0.8924553549162297
1104	16	0.49163164656225433
1104	17	0.3877052960105516
1104	18	0.6154799603479191
1104	19	0.7893806860443604
1104	20	1.0745487926644302
1104	21	1.0620695800821593
1104	22	0.6101244175104483
1104	23	1.0515163697109102
1104	24	1.2174156487887444
1104	25	1.348741908082559
1105	1	0.2875179406383559
1105	2	0.5020556500568425
1105	3	0.18698446577342054
1105	4	0.43014129622672925
1105	5	0.45990181054850154
1105	6	0.729009956755938
1105	7	0.7770374299623377
1105	8	0.5894271416901411
1105	9	0.7089541278232829
1105	10	0.30454303405932137
1105	11	0.9562428902327156
1105	12	1.1610483965716085
1105	13	0.7967938576457101
1105	14	0.6872739595833508
1105	15	0.5454656841977439
1105	16	0.7401358981173516
1105	17	0.7626924557434762
1105	18	0.7548875960267409
1105	19	0.8035106866150343
1105	20	1.0809324027768525
1105	21	0.9992447213250983
1105	22	0.8379598338204417
1105	23	1.37476809601349
1105	24	1.1999563096344872
1105	25	1.2051808740130454
1106	1	-0.03456979409487815
1106	2	0.1942168563657276
1106	3	0.1221723980234195
1106	4	-1.2726373393344481
1106	5	-0.17444874383852493
1106	6	0.13540041069491338
1106	7	0.43608969907456085
1106	8	0.4968290679155878
1106	9	1.0134728919160167
1106	10	0.5723639988034535
1106	11	0.6647943756869736
1106	12	0.7417882396069544
1106	13	0.6041552317564438
1106	14	0.053425995054801945
1106	15	0.5955527896740236
1106	16	0.3582755115904419
1106	17	0.46518792567606226
1106	18	0.42940921508157004
1106	19	-0.892594329378575
1106	20	0.37382226533082274
1106	21	0.10780121752916472
1106	22	0.6819185592255295
1106	23	0.5236399198681525
1106	24	0.5667314260178884
1106	25	0.9184083212690766
1107	1	-0.02519796063467794
1107	2	-0.18616818564838766
1107	3	-0.37465643507318447
1107	4	-0.1701657330377131
1107	5	-0.10767578541235423
1107	6	-0.2561798612439361
1107	7	-0.40420535627137966
1107	8	-0.4766711417888452
1107	9	0.3501434682220861
1107	10	0.28298514482865755
1107	11	0.1633147976235918
1107	12	0.08275957300540426
1107	13	-0.06180688491025421
1107	14	0.07570782604836523
1107	15	-0.05214687898489956
1107	16	-0.21344991940492264
1107	17	-0.4748799662057053
1107	18	-0.21430497386506175
1107	19	-0.011332472602383348
1107	20	-0.10190454762655321
1107	21	-0.01453782235549994
1107	22	-0.0656766435304661
1107	23	0.04147748144324481
1107	24	0.4155296299285567
1107	25	0.0020722595678890343
1108	1	-0.1410282793493174
1108	2	-0.10472953973565424
1108	3	0.018566905000081846
1108	4	-0.15787393094259983
1108	5	0.1494729273035702
1108	6	0.11733387389341488
1108	7	-0.21408427796857765
1108	8	-0.0011894895578095088
1108	9	0.26024918590422175
1108	10	0.15689742857201594
1108	11	0.11098986236422803
1108	12	0.20786975377082229
1108	13	-0.10405976226248725
1108	14	-0.027187359144797085
1108	15	-0.22322128920078654
1108	16	-0.9145123623340439
1108	17	-0.9131658002733829
1108	18	-0.8116383172290753
1108	19	-0.6943866509339855
1108	20	-0.13358143931901623
1108	21	-0.2845495160782221
1108	22	-0.18170210459130232
1108	23	-0.6844673204012182
1108	24	-0.26569064349186533
1108	25	-0.517306959801175
1109	1	0.26042498885014
1109	2	0.1456986381132559
1109	3	0.1219949586332909
1109	4	0.15692051427333809
1109	5	0.28683673266878174
1109	6	0.365219459577105
1109	7	0.37538072089971664
1109	8	0.1592459114507676
1109	9	1.2223619540017268
1109	10	0.3594659295349345
1109	11	0.39159746030231446
1109	12	0.49841371974615356
1109	13	0.5699097663716728
1109	14	0.15589870279898577
1109	15	0.13244844711447712
1109	16	0.20865277805577875
1109	17	0.24879149283670188
1109	18	0.11152733378829538
1109	19	0.3063163499908015
1109	20	0.6980920769721664
1109	21	0.7575830288461844
1109	22	0.43109113576563374
1109	23	0.5875188864386871
1109	24	0.22479819690961023
1109	25	0.35547107495546726
1110	1	-0.14114837257935875
1110	2	-0.06789455736667804
1110	3	-0.13090446016080648
1110	4	0.06550550350814177
1110	5	-0.023701510414113613
1110	6	-0.011047651779307444
1110	7	-0.07238675797047733
1110	8	-0.03139118372703109
1110	9	-3.3050351265998366
1110	10	-1.419800562046909
1110	11	-0.4491779964140221
1110	12	-0.15700952897455522
1110	13	-0.4827914046261661
1110	14	-0.30942715924654607
1110	15	-0.20014125506187241
1110	16	-0.5023617106365208
1110	17	-0.2816398810095677
1110	18	-0.29941976245248725
1110	19	-0.4170130372793892
1110	20	0.11518171546439504
1110	21	-0.20538177969425675
1110	22	-0.03039471555947415
1110	23	0.2148567497555831
1110	24	0.3337033239928928
1110	25	0.373123746459882
1111	1	-0.07766240448410144
1111	2	-0.0701966357764725
1111	3	-0.08377375116696228
1111	4	-0.1386957244060696
1111	5	-0.0568190875592407
1111	6	-0.15340063810888438
1111	7	-0.004901746041348076
1111	8	0.04679333547618114
1111	9	0.7367441600551814
1111	10	0.16672120846237704
1111	11	0.24890191187412825
1111	12	0.3134728051053841
1111	13	0.0694390656181696
1111	14	-0.009239075696321208
1111	15	0.017092846694730213
1111	16	-0.19114131809945434
1111	17	-0.028786922422156636
1111	18	-0.21454604354613593
1111	19	0.01924182437897315
1111	20	0.2640141665411875
1111	21	0.08242318077211053
1111	22	0.28810038242043845
1111	23	0.4458194697061231
1111	24	0.3482955996557564
1111	25	0.6413294915498327
1112	1	-0.19526780468976312
1112	2	-0.3413189696737078
1112	3	-0.15134017568681557
1112	4	-0.20631024658074892
1112	5	-0.2773841520764435
1112	6	-0.3571319380723956
1112	7	-0.11760077081993003
1112	8	-0.5381641229773955
1112	9	0.09487763934001947
1112	10	-0.07752780470642762
1112	11	0.12330069118881681
1112	12	-0.5347479963010571
1112	13	-0.2977997972869275
1112	14	-1.2181682394605389
1112	15	-0.5422733423124697
1112	16	-0.48646340653279907
1112	17	-0.6155289697237123
1112	18	-0.8256719433039876
1112	19	-0.2887046545409859
1112	20	-0.23850805789242457
1112	21	-0.36004580453322177
1112	22	-0.17893282154678758
1112	23	0.02284827854282412
1112	24	-0.022716441343348492
1112	25	0.015340587181235321
1113	1	-1.9691092227264306
1113	2	-2.089960807167788
1113	3	-1.997357021637697
1113	4	-1.9660440264332628
1113	5	-2.3126405623328523
1113	6	-1.8806139419963515
1113	7	-2.0542178806230567
1113	8	-2.3136943645036823
1113	9	-11.356576930716134
1113	10	-4.612733474146928
1113	11	-2.8473034627926452
1113	12	-12.90450055025546
1113	13	-5.07564297433855
1113	14	-3.7923673802493916
1113	15	-3.180262145505761
1113	16	-2.8378114208755356
1113	17	-2.988995751402893
1113	18	-3.1281489408007737
1113	19	-4.157591231903812
1113	20	-15.230754015540754
1113	21	-18.25358565298732
1113	22	-12.412749883991822
1113	23	-17.839326980235338
1113	24	-19.27395451725161
1113	25	-15.650543825240117
1114	1	0.0436021389616883
1114	2	0.11758596853775316
1114	3	0.11251596500592953
1114	4	-0.2901611908655113
1114	5	0.1512606409603734
1114	6	0.152869726402038
1114	7	0.14699159533196493
1114	8	0.3142723022186864
1114	9	1.1487911841022012
1114	10	0.3280376294372189
1114	11	0.083564408893956
1114	12	0.14868013088102572
1114	13	0.08735471465669065
1114	14	0.24857901212679678
1114	15	0.3301448954556747
1114	16	0.21195371887926484
1114	17	0.3601581334412245
1114	18	0.24690056282614847
1114	19	0.11685854677111251
1114	20	-0.22001026636581145
1114	21	-0.3408014353284372
1114	22	-0.6121922684541161
1114	23	-0.08254644547919554
1114	24	0.21207919367394368
1114	25	-0.08281367306137355
1115	1	-0.2792584412196959
1115	2	-0.29772690071888164
1115	3	-0.2547700891832356
1115	4	-0.1878274697847928
1115	5	-0.5807784099749327
1115	6	-0.5166174898040197
1115	7	-0.7233130301446451
1115	8	-0.5683229454125183
1115	9	-0.20502779581330088
1115	10	-0.5210950697670462
1115	11	-0.4844412242919134
1115	12	-0.5036908078224656
1115	13	-0.641927475332384
1115	14	-0.7375334923052392
1115	15	-0.5586117449416861
1115	16	-0.5533742365331094
1115	17	-0.5050047549219912
1115	18	-0.6678839080824588
1115	19	-0.6753869011537006
1115	20	-1.0802138682046412
1115	21	-0.4564511776768043
1115	22	-1.1752246393621881
1115	23	-0.771281337306867
1115	24	-0.37873448617638417
1115	25	-0.304542446955125
1116	1	-0.5829905339379131
1116	2	-0.4722542773039322
1116	3	-0.46883877877863966
1116	4	-0.31604514445123755
1116	5	-1.3041659860616654
1116	6	-0.9692951279898239
1116	7	-2.3908802455824585
1116	8	-1.4674130520144608
1116	9	-0.4065906663655774
1116	10	-1.492696675383776
1116	11	-0.938248635191016
1116	12	-1.8348232741305743
1116	13	-1.5055184910225705
1116	14	-1.1161885101503017
1116	15	-2.175781289167876
1116	16	-2.472551753214365
1116	17	-3.1456005224647576
1116	18	-2.414640249009583
1116	19	-1.846852509510871
1116	20	-2.2422152969810156
1116	21	-1.5275591527669121
1116	22	-1.4201152267122445
1116	23	-1.5439122913957135
1116	24	-1.9521865475083686
1116	25	-1.53242203155596
1201	1	0.9153767495327756
1201	2	0.970390039971079
1201	3	0.5303288433675242
1201	4	0.847000739069685
1201	5	1.2007680474060294
1201	6	1.2776098873804358
1201	7	1.3512859617135895
1201	8	1.2856449807628998
1201	9	2.0149082203797732
1201	10	1.7398975069849385
1201	11	1.6849332482342163
1201	12	1.7066804911136657
1201	13	1.491238076154616
1201	14	1.8330285377683495
1201	15	1.6055771634508815
1201	16	1.4070851702726586
1201	17	1.5724062219958839
1201	18	1.4926560971570808
1201	19	1.4963135764634856
1201	20	1.7793671479414996
1201	21	1.7249253787177778
1201	22	1.6901450424852058
1201	23	2.0976100992908826
1201	24	2.049372478910712
1201	25	2.151058653518568
1202	1	0.8566531109870539
1202	2	0.9500520385044453
1202	3	0.8994132347597841
1202	4	1.0041929779986702
1202	5	1.248384387093985
1202	6	1.0549228451978294
1202	7	1.268123981501244
1202	8	1.2761604011161758
1202	9	1.998310562901061
1202	10	1.5090193018170766
1202	11	1.601714163533508
1202	12	1.4768308805424866
1202	13	1.5082568792350202
1202	14	1.6574384223649332
1202	15	1.5848613206195594
1202	16	1.521037414740782
1202	17	1.658791750576441
1202	18	1.4957397395288012
1202	19	1.6027106591855258
1202	20	1.7543756065438387
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2211	4	0.12775097659590529
2211	5	-0.41803579445330286
2211	6	0.09241403561096462
2211	7	0.41775899175124565
2211	8	0.317558056348922
2211	9	0.8439333932891202
2211	10	0.030300429376509896
2211	11	0.5480585974398267
2211	12	0.6795431757222374
2211	13	0.4882038059688725
2211	14	0.3047232928883332
2211	15	0.35988097033431643
2211	16	0.43066734017762087
2211	17	0.2892698753041145
2211	18	0.276755830720397
2211	19	-0.004957509799481841
2211	20	0.41678060472058576
2211	21	0.8132406382168327
2211	22	0.5639362708831541
2211	23	0.9046345443472816
2211	24	0.9272353007664904
2211	25	1.2128902928081047
2212	1	-0.01817985287295798
2212	2	-0.036393977818132583
2212	3	-0.0023622013834625477
2212	4	0.13103471142581213
2212	5	-0.10284818551227914
2212	6	0.22761862409139866
2212	7	0.12461008231825232
2212	8	0.052757726322976595
2212	9	0.6563712072327164
2212	10	0.31420799022892965
2212	11	0.3867429611386797
2212	12	0.28233373794444816
2212	13	0.13885328209934045
2212	14	0.2283659114933485
2212	15	0.3342157958089871
2212	16	0.42261245975564776
2212	17	0.15855259766046004
2212	18	0.38976104200426676
2212	19	0.4250692523186075
2212	20	0.7046892635858129
2212	21	0.7451911584315027
2212	22	0.5016735207732168
2212	23	0.8210190611224988
2212	24	1.0948838542395976
2212	25	0.9544406682281732
2213	1	-0.16116428383250891
2213	2	-0.18693224942877862
2213	3	-0.3080996475582296
2213	4	-0.26510282216437275
2213	5	-0.30051369666121275
2213	6	0.06395493525328533
2213	7	-0.1545219342336317
2213	8	-0.15590235489473514
2213	9	0.6123958176790936
2213	10	-0.08482305920318112
2213	11	0.18199759130362736
2213	12	0.23311939749917343
2213	13	-0.15281319185256592
2213	14	0.05949632234580804
2213	15	0.03873029335534284
2213	16	-0.1367504329392304
2213	17	-0.1785599685756054
2213	18	-0.34994480974103226
2213	19	-0.3215356916630654
2213	20	0.18943396277347802
2213	21	0.044283510430418005
2213	22	0.05029603417460038
2213	23	-0.20808838429682197
2213	24	0.08153633324524634
2213	25	0.3555864731360785
2214	1	-1.1334873370953389
2214	2	-0.7623210296292555
2214	3	-0.872872712917026
2214	4	-0.7329125202760451
2214	5	-0.9468900337916253
2214	6	-0.7500106678697307
2214	7	-0.6385254856399953
2214	8	-0.6641564446092545
2214	9	-9.888817347843343
2214	10	-3.5418958010511723
2214	11	-1.596707982308228
2214	12	-0.977023259797253
2214	13	-0.6941840332060494
2214	14	-0.9164651944435462
2214	15	-0.8256470422559374
2214	16	-0.9910903687178987
2214	17	-0.9352686292889558
2214	18	-1.0783453358029647
2214	19	-1.3035530813080136
2214	20	-0.929446867145181
2214	21	-1.4919523669630408
2214	22	-1.1668166431515417
2214	23	-1.501399276227989
2214	24	-1.828518778541035
2214	25	-9.464332356154497
2215	1	-0.6930524867362813
2215	2	-0.4243255406329247
2215	3	-0.36037107824139625
2215	4	-0.6413714326865048
2215	5	-0.5265062385940702
2215	6	-0.46793384507903824
2215	7	-0.6722793056864873
2215	8	-0.5987095006885141
2215	9	0.24767982013662504
2215	10	0.21666249122480963
2215	11	0.0731852232460497
2215	12	0.06380002407843932
2215	13	0.03553591682872792
2215	14	-0.23272583058498242
2215	15	-0.6943955121574845
2215	16	-0.3046984135561033
2215	17	-0.45275220377842373
2215	18	-0.3966665746398448
2215	19	-0.4164285671186576
2215	20	-0.29434722414244874
2215	21	-0.34018978234433206
2215	22	-0.41030081907077687
2215	23	0.014892001610220973
2215	24	0.09698572278778528
2215	25	0.2122178935641159
2216	1	-0.2644255036402079
2216	2	-0.30434872920605827
2216	3	-0.36350859161170845
2216	4	-0.11998213887958542
2216	5	-1.052421794342063
2216	6	-1.5150783310576479
2216	7	-0.8651822552537496
2216	8	-1.7585534990014864
2216	9	-0.4130028796803984
2216	10	-0.8135171320170009
2216	11	-0.3385699079874769
2216	12	-1.4714342590883582
2216	13	-1.2446489182293128
2216	14	-1.4283333118665453
2216	15	-1.0232579089835667
2216	16	-2.1066429822823203
2216	17	-2.3579324283836485
2216	18	-1.91335240475388
2216	19	-3.108553753340857
2216	20	-1.9577360593095428
2216	21	-2.297400771098033
2216	22	-2.0251331669699333
2216	23	-1.6768467083930965
2216	24	-1.667752090783143
2216	25	-1.8746526593534618
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	75632.0
3	2.0
4	1035.0
5	11037.0
6	15162.0
7	11602.0
8	9224.0
9	7178.0
10	6347.0
11	5697.0
12	4980.0
13	4642.0
14	4581.0
15	4591.0
16	4722.0
17	5009.0
18	5863.0
19	7553.0
20	10170.0
21	13670.0
22	16191.0
23	19101.0
24	21690.0
25	24629.0
26	27346.0
27	29505.0
28	32778.0
29	38147.0
30	45535.0
31	56649.0
32	69697.0
33	86542.0
34	107124.0
35	133461.0
36	175280.0
37	256659.0
38	571103.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.73501427088266	18.353047936517363	12.99366600977369	22.918271782826285
2	16.615367629213683	20.90677949330092	38.18153505468946	24.296317822795938
3	19.526241397735784	26.37024291013023	29.156923423052767	24.94659226908122
4	12.24863297901983	16.85882302084684	34.61329933423644	36.2792446658969
5	12.521640550392851	39.2916330846309	32.80142167279957	15.385304692176685
6	31.20969543932459	36.92474606922293	16.696173212015335	15.169385279437142
7	27.933936646398223	33.905808776532986	19.639970766768087	18.520283810300704
8	27.7995445900138	34.633351201741625	18.142685752002123	19.424418456242456
9	27.30036291494787	15.541366364880085	18.712938756669853	38.4453319635022
10	16.466352854139693	31.042171923069912	29.304133312146213	23.187341910644186
11	34.71965621193317	23.454323974820277	20.927598391515335	20.898421421731214
12	24.385002450562954	28.029388005106338	27.9008441290918	19.684765415238907
13	29.698641352324994	21.5394118238336	25.889667621666074	22.872279202175335
14	22.479390753807788	21.64564596471272	28.16768853527999	27.707274746199502
15	24.653127167955198	28.237167267704578	24.81786572167257	22.29183984266765
16	22.29207303258132	29.085217699280975	25.82797082238478	22.79473844575292
17	21.980311987291334	28.10880491679471	27.3906091304461	22.52027396546785
18	20.85874775111702	25.27239220727539	31.613070242238685	22.255789799368905
19	22.352400667167423	25.12347910517888	31.847295336182995	20.676824891470698
20	22.556785360271316	24.28366870384	32.050606906804134	21.108939029084542
21	23.07976379786039	23.831633314773153	32.0158384816559	21.07276440571056
22	22.648310116797994	23.967183639113784	31.97402359294462	21.410482651143603
23	21.7484468296992	24.07361881388207	32.682359485484994	21.495574870933737
24	22.032980225510535	24.444127314649645	32.03819376989259	21.484698689947233
25	21.635527053272334	24.14499667618191	32.597557319568814	21.621918950976944
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	3437.0
1	3437.0
2	3355.5
3	3274.0
4	3274.0
5	3274.0
6	8457.5
7	13641.0
8	13641.0
9	13641.0
10	13951.5
11	14262.0
12	14262.0
13	14262.0
14	12769.5
15	11277.0
16	11277.0
17	11277.0
18	18066.0
19	24855.0
20	24855.0
21	24855.0
22	38866.5
23	52878.0
24	52878.0
25	52878.0
26	76761.5
27	100645.0
28	100645.0
29	100645.0
30	116008.5
31	131372.0
32	131372.0
33	131372.0
34	143164.5
35	154957.0
36	154957.0
37	154957.0
38	161761.5
39	168566.0
40	168566.0
41	168566.0
42	183232.0
43	197898.0
44	197898.0
45	197898.0
46	214498.5
47	231099.0
48	231099.0
49	231099.0
50	230026.5
51	228954.0
52	228954.0
53	228954.0
54	215208.5
55	201463.0
56	201463.0
57	201463.0
58	177799.5
59	154136.0
60	154136.0
61	154136.0
62	130529.0
63	106922.0
64	106922.0
65	106922.0
66	83585.5
67	60249.0
68	60249.0
69	60249.0
70	45865.5
71	31482.0
72	31482.0
73	31482.0
74	22863.0
75	14244.0
76	14244.0
77	14244.0
78	11604.5
79	8965.0
80	8965.0
81	8965.0
82	6243.5
83	3522.0
84	3522.0
85	3522.0
86	2490.0
87	1458.0
88	1458.0
89	1458.0
90	993.5
91	529.0
92	529.0
93	529.0
94	288.0
95	47.0
96	47.0
97	47.0
98	24.5
99	2.0
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.025571132014744802
2	0.0010415939720873648
3	0.0
4	0.004322614984162564
5	0.03671618751607961
6	0.011509613391565381
7	0.02088395914035166
8	0.028383435739380687
9	0.03520587625655293
10	0.037966100282584445
11	0.042288715266747004
12	0.008697309666929496
13	0.0032810210120751988
14	0.006353723229732925
15	0.006353723229732925
16	0.008801469064138232
17	0.009582664543203756
18	0.015207271992475525
19	0.01958196667524246
20	0.01650926445758473
21	0.013176163746905162
22	0.012394968267839642
23	0.009895142734829965
24	0.0070307593115897125
25	0.11244006928683102
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1920134.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	35.82601350688891
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.74785003714928	25.704394445211502
2	11.504726047858634	8.243369415672804
3	5.250441690379798	5.64307184750038
4	2.9490757701875836	4.2261451350231685
5	1.8905139378158853	3.3864788935576833
6	1.2908088710106387	2.77467216285835
7	0.8903248894931284	2.232775406155014
8	0.6689816066949376	1.9173555261850463
9	0.5174147903164209	1.668321833988617
>10	2.9117995964759116	18.869674788829972
>50	0.22397106947584364	5.5555361739031115
>100	0.14217166115061988	9.455743786528842
>500	0.007849777165005529	1.8799972990139333
>1k	0.003052691119724372	1.9972269707522188
>5k	2.9073248759279734E-4	0.7193141830516204
>10k+	7.268312189819934E-4	5.725922131767776
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	34654	1.8047698754357768	No Hit
GGTATCAACGCAGAGTACTTTTTTT	26537	1.38203896186412	No Hit
TATCAACGCAGAGTACTTTTTTTTT	25766	1.341885514240152	No Hit
ACGCAGAGTACTTTTTTTTTTTTTT	12150	0.6327683380430741	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	10840	0.5645439328713517	No Hit
GAGTACTTTTTTTTTTTTTTTTTTT	8558	0.4456980606561834	No Hit
GTGGTATCAACGCAGAGTACTTTTT	5254	0.2736267364673507	No Hit
ATCAACGCAGAGTACTTTTTTTTTT	4139	0.21555787252348016	No Hit
GTGGTATCAACGCAGAGTACATGGG	4039	0.21034990266304332	No Hit
GCAGAGTACTTTTTTTTTTTTTTTT	3238	0.16863406408094433	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA	2232	0.1162418872849499	No Hit
TGCATGCACTGCCTCAGTGACCAGT	2009	0.10462811449617579	No Hit
GTATAACGCAGAGTACTTTTTTTTT	1975	0.10285740474362728	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	1.0415939720873647E-4	0.0	0.0	0.0	0.0
3	1.0415939720873647E-4	0.0	0.0	0.0	0.0
4	1.0415939720873647E-4	0.0	0.0	0.0	0.0
5	1.562390958131047E-4	0.0	0.0	0.0	0.0
6	2.0831879441747295E-4	0.0	0.0	0.0	0.0
7	2.0831879441747295E-4	0.0	0.0	5.207969860436824E-5	0.0
8	2.0831879441747295E-4	0.0	0.0	5.207969860436824E-5	0.0
9	2.0831879441747295E-4	0.0	0.0	5.207969860436824E-5	0.0
10	2.0831879441747295E-4	0.0	0.0	5.207969860436824E-5	0.0
11	2.0831879441747295E-4	0.0	0.0	1.562390958131047E-4	0.0
12	3.124781916262094E-4	0.0	0.0	2.0831879441747295E-4	0.0
13	3.124781916262094E-4	0.0	0.0	2.603984930218412E-4	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATAACG	245	0.0	15.896628	2
ATAACGC	250	0.0	15.200708	3
GCGAGAC	65	3.3824454E-6	14.611497	13
TAACGCA	275	0.0	14.509768	4
GACCGGT	40	0.0052735093	14.252521	7
GTTTCGA	40	0.0052908557	14.245467	17
GTATAAC	375	0.0	14.189547	1
GCCCCCG	235	0.0	14.144069	18
ATTCGCC	55	1.9601878E-4	13.818466	11
TATCACG	110	1.8735591E-10	13.817025	2
CGTAAGA	50	0.0015007171	13.298888	2
GGTATCA	6190	0.0	13.063233	1
ACCGAGT	110	2.741217E-9	12.956838	8
ACGGCCC	245	0.0	12.792515	15
CCGTAAG	60	4.085801E-4	12.669239	1
CGCGCCA	120	7.403287E-10	12.667917	10
GGCCCCC	255	0.0	12.662638	17
TCCCGAT	130	1.9826984E-10	12.419126	17
GTGGTAT	1865	0.0	12.176733	1
TGACCGA	180	0.0	12.146734	19
>>END_MODULE