FastQCFastQC Report
Thu 26 May 2016
SRR1364311_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364311_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2007221
Sequences flagged as poor quality0
Sequence length25
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNN53190.26499324190011964No Hit
GTATCAACGCAGAGTACTTTTTTTT26390.13147530839902533No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTAAC456.800472E-414.7671642
CGCGCCA1550.012.87008610
TCCCGAC1052.0147127E-812.657572
AATACCG550.003061358612.0949215
ACGGCCC2250.011.83265915
TTGCGCG2250.011.83236218
CAACGCG658.002879E-411.6961874
TTAGCAC2700.011.6027723
TACACTA1354.7057256E-911.2629965
GGTATCA10900.011.2350451
TGCGCGC2550.011.18605619
TTGACCG2400.011.09283818
TCGGGAC600.005908169311.0753733
GCCCCCG2600.010.9709418
GTATCAA26100.010.948071
GTTAACC700.001500693710.8493453
GTTCTAG1803.6379788E-1110.5479751
AACTGGC3900.010.48334317
CAACCGT1002.4004663E-510.4495476
ATTTGCG2550.010.4405816
Produced by FastQC (version 0.11.5)