FastQCFastQC Report
Thu 26 May 2016
SRR1364311_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364311_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2007221
Sequences flagged as poor quality0
Sequence length25
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT51090.2545310157675712No Hit
GGTATCAACGCAGAGTACTTTTTTT37880.18871863138139747No Hit
TATCAACGCAGAGTACTTTTTTTTT37610.18737348802149836No Hit
GTGGTATCAACGCAGAGTACATGGG24150.12031560052430698No Hit
ACGCAGAGTACTTTTTTTTTTTTTT22740.11329096297816733No Hit
TGCATGCACTGCCTCAGTGACCAGT22200.11060067625836915No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTCGG250.00603359118.99950210
GTCGGCG350.002175579816.2800112
TCGGCGT456.77512E-414.77444413
GGGCGTA905.4043994E-712.6672829
CGCGGTT1207.4396667E-1012.66286218
GGTATCA19150.012.4039281
TAGACCG550.003059854712.0957175
CTCGCGG550.003074170812.088181514
ACGCTAC658.0152345E-411.694041
CCTACGC658.018588E-411.6934583
CTTCGCG1157.099152E-811.56260915
TTCGCGG1354.7475623E-911.25671916
GTGGTAT7100.011.2411031
TAATACC600.005865828611.0860824
TAGACAG3700.011.044935
TGGACGC1752.0008883E-1110.8614615
TTGACCG2100.010.85388218
TAGGCGG1158.774423E-710.7434025
TTATACC1158.7913577E-710.7417944
GGTCTAG803.7665403E-410.6890851
Produced by FastQC (version 0.11.5)