##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1364311_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2007221
Sequences flagged as poor quality	0
Sequence length	25
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.83173003869529	31.0	30.0	34.0	26.0	34.0
2	29.864356739990264	31.0	30.0	34.0	26.0	34.0
3	29.930681773456932	31.0	30.0	34.0	26.0	34.0
4	33.33850931212856	35.0	33.0	37.0	28.0	37.0
5	32.85642936178926	35.0	33.0	37.0	26.0	37.0
6	32.77014439366667	35.0	33.0	37.0	26.0	37.0
7	32.50822256243832	35.0	33.0	37.0	25.0	37.0
8	32.41176781231364	35.0	33.0	37.0	23.0	37.0
9	33.22727243288108	37.0	33.0	39.0	18.0	39.0
10	33.34739373492007	37.0	33.0	39.0	19.0	39.0
11	33.31756443361244	37.0	33.0	39.0	19.0	39.0
12	33.16679777662748	37.0	32.0	39.0	18.0	39.0
13	33.28528597498731	37.0	33.0	39.0	19.0	39.0
14	34.253434973029876	38.0	33.0	41.0	19.0	41.0
15	34.28054409554304	38.0	33.0	41.0	19.0	41.0
16	34.22111067989026	38.0	33.0	41.0	18.0	41.0
17	34.13839233447637	38.0	33.0	41.0	18.0	41.0
18	34.07044914336787	38.0	33.0	41.0	17.0	41.0
19	33.947799470013514	39.0	33.0	41.0	18.0	41.0
20	33.77933321741851	39.0	33.0	41.0	16.0	41.0
21	33.702957472047174	39.0	33.0	41.0	15.0	41.0
22	33.6800935223376	39.0	33.0	41.0	11.0	41.0
23	33.37467174765509	38.0	32.0	41.0	8.0	41.0
24	33.27153163503172	38.0	32.0	41.0	2.0	41.0
25	33.130801740316585	38.0	32.0	41.0	2.0	41.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1101	1	0.24847763101376685
1101	2	0.3747673682146342
1101	3	-1.5624842018925413
1101	4	-0.599761942715709
1101	5	0.2675951626035058
1101	6	0.7691468387024756
1101	7	0.914873640093333
1101	8	0.9270460017999298
1101	9	1.6895377614689906
1101	10	1.4019824952044573
1101	11	1.2174378295011934
1101	12	1.4033718971367435
1101	13	1.1964070581238317
1101	14	1.3407625527991627
1101	15	1.377675367551113
1101	16	1.2691801477766091
1101	17	1.2072195041649394
1101	18	1.1980527848700078
1101	19	1.3682758395889394
1101	20	1.5969884064962443
1101	21	1.356665432408846
1101	22	1.3872931005479003
1101	23	1.6991166128872877
1101	24	1.5874826959890171
1101	25	1.7620671233442877
1102	1	0.5623317423741732
1102	2	0.6386025619143538
1102	3	0.6520202721731394
1102	4	0.6770727200439026
1102	5	0.7664554319349364
1102	6	0.8586834260810505
1102	7	1.037470201905819
1102	8	1.0861256225747908
1102	9	1.7148508370757938
1102	10	1.2065163500320466
1102	11	1.3337098203086057
1102	12	1.3380413518877177
1102	13	1.3067164825442745
1102	14	1.2796989910162608
1102	15	1.3474911889434793
1102	16	1.3219658706258741
1102	17	1.215577529067744
1102	18	1.4027138473418859
1102	19	1.272275328889954
1102	20	1.382066653989071
1102	21	1.6481235247301456
1102	22	1.6393168231386994
1102	23	1.8550067005001907
1102	24	1.898179192874224
1102	25	1.7457893716031592
1103	1	-2.44833423104361
1103	2	-2.4821081878670377
1103	3	-2.3142862565466977
1103	4	-2.2918680332340315
1103	5	-5.202036891121466
1103	6	-10.0711936010205
1103	7	-10.343389463068327
1103	8	-10.951514034654846
1103	9	-11.530433828246796
1103	10	-9.494208591959868
1103	11	-13.002020796749555
1103	12	-11.952390540954426
1103	13	-12.729311249834993
1103	14	-12.834019883229967
1103	15	-13.084313785117253
1103	16	-7.671355549007252
1103	17	-5.320790464891779
1103	18	-4.394950800782798
1103	19	-3.903176025217821
1103	20	-9.210382993053926
1103	21	-5.831588340672916
1103	22	-11.63718403948349
1103	23	-13.278727311295949
1103	24	-14.184611327795682
1103	25	-14.593112980292151
1104	1	0.14758030414904866
1104	2	0.43366029168948117
1104	3	0.4389564322940025
1104	4	0.6298574434454167
1104	5	0.4822733777721808
1104	6	0.5812307262666749
1104	7	0.49167202774275864
1104	8	0.7787773106750606
1104	9	0.9912716621238715
1104	10	0.8348468335450505
1104	11	0.7828583040341002
1104	12	0.8043072660568242
1104	13	0.6616293273983302
1104	14	0.7696605895479607
1104	15	0.7013934908565673
1104	16	0.5041038967377034
1104	17	0.4207997272161421
1104	18	0.4580421190374011
1104	19	0.7244180061762435
1104	20	0.9314791085962
1104	21	0.9108030989636475
1104	22	0.5837287005317364
1104	23	0.9846677043340648
1104	24	1.199896530294346
1104	25	1.2600450958520923
1105	1	0.4619530767851536
1105	2	0.4564705492579719
1105	3	0.028905877002085134
1105	4	0.4085865782321747
1105	5	0.5869575774353422
1105	6	0.6045910311633449
1105	7	0.7246717375986051
1105	8	0.5226550999288975
1105	9	0.8298048565602087
1105	10	0.425545958290968
1105	11	0.8930748040159102
1105	12	1.0232814034701079
1105	13	0.8747962465665395
1105	14	0.8385716227233644
1105	15	0.6131260151698896
1105	16	0.779634467188167
1105	17	0.7696299725867348
1105	18	0.8471146390027826
1105	19	0.719433955645485
1105	20	0.9454753917058341
1105	21	1.032936339417013
1105	22	0.7404100308976425
1105	23	1.348073992426059
1105	24	1.2714707780131604
1105	25	1.3359010845373192
1106	1	-0.14483204343439127
1106	2	0.08410473211093006
1106	3	-0.09386991239456322
1106	4	-1.399424724122074
1106	5	-0.31789631077219127
1106	6	-0.0713924468606848
1106	7	0.271166174424728
1106	8	0.4213432641769046
1106	9	0.8009079969018345
1106	10	0.38470771041023966
1106	11	0.4799132047302024
1106	12	0.7422810614807673
1106	13	0.5675966973500906
1106	14	-0.01171852930937689
1106	15	0.5222593508097333
1106	16	0.21201709602669894
1106	17	0.3205013987146117
1106	18	0.3454382661117492
1106	19	-1.2144368603337483
1106	20	0.16002837995220176
1106	21	-0.2977067783789664
1106	22	0.41136789313338795
1106	23	0.39095620054117575
1106	24	0.41946647582391705
1106	25	0.8552380326322151
1107	1	-0.09027839397446158
1107	2	-0.1827007489354493
1107	3	-0.26937952871243454
1107	4	-0.14129933570225717
1107	5	0.14723572183361
1107	6	0.0387680129480259
1107	7	-0.04890560672080824
1107	8	-0.3087337462967632
1107	9	0.6098909803339794
1107	10	0.41599899075403357
1107	11	0.35059211023795456
1107	12	0.26099816540145326
1107	13	0.24971708946723226
1107	14	0.3913426682088783
1107	15	0.38882140271943655
1107	16	-0.06139694052193079
1107	17	-0.20950824160360781
1107	18	-0.041579910931353936
1107	19	0.17831644965333737
1107	20	1.60121804825053E-4
1107	21	0.029183852115608033
1107	22	0.13000762498917595
1107	23	0.3981579982628105
1107	24	0.6276457540243854
1107	25	0.4170503617089949
1108	1	-0.21572635721768307
1108	2	-0.1301510543239246
1108	3	0.06050608013664416
1108	4	-0.10468036525426783
1108	5	0.128021921199597
1108	6	0.03205212563522508
1108	7	-0.44669728326248404
1108	8	-0.04784181061648951
1108	9	0.17958204213111628
1108	10	-0.007732503755391917
1108	11	0.1060377878951968
1108	12	0.15932303981557538
1108	13	-0.25067903655951795
1108	14	-0.20539037893729528
1108	15	-0.3920651893747973
1108	16	-0.8867531637631103
1108	17	-0.9676742519645103
1108	18	-0.8165093120719504
1108	19	-0.8997261812734934
1108	20	-0.2965596930015124
1108	21	-0.5542085905933192
1108	22	-0.4057468114851446
1108	23	-0.8288188383891253
1108	24	-0.4205710351768417
1108	25	-0.5647033601767646
1109	1	0.2470471680639399
1109	2	0.2742057153689714
1109	3	0.22127644328816487
1109	4	0.3232754965084226
1109	5	0.20564203793935576
1109	6	0.286337757680883
1109	7	0.4496290534981995
1109	8	0.35257401565962
1109	9	1.2612442575548286
1109	10	0.5625234272945505
1109	11	0.6380902323780191
1109	12	0.6151264120690101
1109	13	0.640584396839408
1109	14	0.34697340583225866
1109	15	0.027307380688583294
1109	16	0.34589788551718925
1109	17	0.21372870168704594
1109	18	0.25683761143040584
1109	19	0.4484507623159999
1109	20	0.7296683052138775
1109	21	0.7555343886760184
1109	22	0.4115071519714846
1109	23	0.7344538840239636
1109	24	0.686568532363836
1109	25	0.6928537812411761
1110	1	-0.11107665171538983
1110	2	-0.039388085838950815
1110	3	-0.19677605604857007
1110	4	-0.015505579384644363
1110	5	-0.010104641836541361
1110	6	0.029864686219980285
1110	7	-0.09806863834130297
1110	8	-0.11673322406758047
1110	9	-3.7766753547865726
1110	10	-1.8017149109547468
1110	11	-0.7654402855925468
1110	12	-0.33701632245351476
1110	13	-0.7239684068186918
1110	14	-0.41047856601411326
1110	15	-0.29743199537458764
1110	16	-0.7811402873061368
1110	17	-0.44596432389155183
1110	18	-0.3345657002102769
1110	19	-0.5607615915707314
1110	20	-0.26088758847652826
1110	21	-0.44681122675541474
1110	22	-0.24846015298973612
1110	23	-0.07859018795193862
1110	24	0.16567296921324726
1110	25	0.20445622829706167
1111	1	0.0190773145802261
1111	2	0.10944985061837542
1111	3	-0.01788650483968368
1111	4	-0.10316536408443966
1111	5	-0.0912361462425082
1111	6	0.05667728662692184
1111	7	0.2224081781483065
1111	8	0.22663618260151708
1111	9	0.7873811934788435
1111	10	0.28046552349636755
1111	11	0.36576318835286514
1111	12	0.4659831399890919
1111	13	0.3361760251494914
1111	14	0.06014523160553864
1111	15	0.0901120373532649
1111	16	0.2560187526687301
1111	17	0.3188743395774978
1111	18	0.16929500927307117
1111	19	0.1989344651608249
1111	20	0.4460415879516617
1111	21	0.3009380404568205
1111	22	0.4028532044643711
1111	23	0.421148542211192
1111	24	0.41315403533280914
1111	25	0.6064525069828335
1112	1	-0.06847851279561823
1112	2	-0.10555885505450391
1112	3	-0.08778172268708317
1112	4	-0.09596041330046745
1112	5	-0.002589896934196645
1112	6	-0.12981598610647183
1112	7	0.03941630477545033
1112	8	-0.4722132066879645
1112	9	0.07729009370880391
1112	10	-2.8257618858162914E-4
1112	11	0.21933571211857839
1112	12	-0.18687494183909337
1112	13	-0.20741801010834138
1112	14	-0.9949534831299189
1112	15	-0.2608901857483801
1112	16	-0.4123696328386828
1112	17	-0.3736669345629693
1112	18	-0.39946153283023733
1112	19	-0.05131751110400984
1112	20	-0.13621031750285084
1112	21	-0.41816681421926205
1112	22	-0.022712007564017256
1112	23	0.21152741743483006
1112	24	0.17698731812812696
1112	25	-0.0059982898332151535
1113	1	-2.067665391118368
1113	2	-2.0089741171602427
1113	3	-2.1041439940060513
1113	4	-2.0403304486199687
1113	5	-1.9214471925005583
1113	6	-1.7453957874553971
1113	7	-2.1461841768628496
1113	8	-2.2431993608229277
1113	9	-10.97267026643777
1113	10	-4.401291363423301
1113	11	-3.0236232872405076
1113	12	-12.94675891578899
1113	13	-5.1403831933669615
1113	14	-4.156621188686213
1113	15	-3.4779354463174172
1113	16	-3.217349656357552
1113	17	-3.1664340654958885
1113	18	-3.4366138189435986
1113	19	-4.002313643523227
1113	20	-14.924283425120414
1113	21	-17.126635295900954
1113	22	-11.795962844958499
1113	23	-17.918096482547224
1113	24	-19.104694455353286
1113	25	-15.255480664620922
1114	1	-0.08685197206369466
1114	2	-0.09178352275485935
1114	3	-0.011160067031074306
1114	4	-0.47919095923163013
1114	5	-0.1205925370897063
1114	6	0.007960045079940414
1114	7	-0.20078548658170092
1114	8	-0.009143573618530354
1114	9	0.7975032354075324
1114	10	-0.10320754119732811
1114	11	-0.08282922290784711
1114	12	-0.2791258376865784
1114	13	-0.09033251808942566
1114	14	-0.26350710655005827
1114	15	-0.19359821810436273
1114	16	-0.2945984848514698
1114	17	-0.14052485673731496
1114	18	-0.2738749202278328
1114	19	-0.5865557425376196
1114	20	-0.6819303491666986
1114	21	-0.9619455771446326
1114	22	-1.2196033689556671
1114	23	-0.6448325835710875
1114	24	-0.2751005591890987
1114	25	-0.6844985508835677
1115	1	-0.32070739358103495
1115	2	-0.28743582657721234
1115	3	-0.34677470204506733
1115	4	-0.21316853370626632
1115	5	-0.5958782695539924
1115	6	-0.5675895377721005
1115	7	-0.9568690553743835
1115	8	-0.5863982008428579
1115	9	-0.27275106068560717
1115	10	-0.5186337548352924
1115	11	-0.4699634055586799
1115	12	-0.561625906232841
1115	13	-0.6987850106073381
1115	14	-0.785014463497518
1115	15	-0.8319026085101342
1115	16	-0.7376908493601135
1115	17	-0.5976048772941596
1115	18	-0.7457252545578399
1115	19	-0.5879154191961078
1115	20	-1.0430900675856734
1115	21	-0.42757226224028244
1115	22	-0.9686155997182482
1115	23	-0.728333533024724
1115	24	-0.20179841499165718
1115	25	-0.2739841164464849
1116	1	-0.5906217902463808
1116	2	-0.5513433059922335
1116	3	-0.5075809961375732
1116	4	-0.3410343014517139
1116	5	-1.1919736532445668
1116	6	-1.1974341790648424
1116	7	-2.3940259690938603
1116	8	-1.5089901056519643
1116	9	-0.48362295160193014
1116	10	-1.5813476971187335
1116	11	-1.1696424430940269
1116	12	-2.1102152742791382
1116	13	-1.775416080074958
1116	14	-1.2933897679627009
1116	15	-2.454816308454422
1116	16	-2.7964306313317415
1116	17	-3.347775454656702
1116	18	-2.4946481872170487
1116	19	-2.010841352992074
1116	20	-2.557997672381667
1116	21	-1.7178001369432252
1116	22	-1.3832502967470717
1116	23	-1.5298617876976444
1116	24	-2.0907237688005758
1116	25	-1.5608378340081615
1201	1	0.8398995405390188
1201	2	0.9287226594926032
1201	3	0.5149300272579787
1201	4	0.9752902233159304
1201	5	1.1261273802568041
1201	6	1.1420024952293488
1201	7	1.3192247043682173
1201	8	1.3133940929519667
1201	9	2.0387089878717646
1201	10	1.750481752386058
1201	11	1.6872038376595953
1201	12	1.959155397761208
1201	13	1.688559673498773
1201	14	1.840860100446676
1201	15	1.756178963522622
1201	16	1.744384899595282
1201	17	1.766382036806995
1201	18	1.7023412984495891
1201	19	1.8368991302224487
1201	20	1.9764797301877692
1201	21	2.026008032922803
1201	22	1.9405738798935062
1201	23	2.4327788266502424
1201	24	2.2374563095815176
1201	25	2.309959550942324
1202	1	0.8961348616105163
1202	2	0.9013348551506297
1202	3	0.9393854715543739
1202	4	1.098897037134094
1202	5	1.0987457146872615
1202	6	0.9762565165363739
1202	7	1.247815653296371
1202	8	1.188410330553154
1202	9	1.885923930959315
1202	10	1.5511481380577266
1202	11	1.6002850684176977
1202	12	1.637656813789654
1202	13	1.5378087426799993
1202	14	1.540515397412534
1202	15	1.5064084275474983
1202	16	1.478985049008621
1202	17	1.4882519068897437
1202	18	1.4634974485403802
1202	19	1.399847253758665
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2210	25	1.2793353279223112
2211	1	0.018633721148987092
2211	2	0.06163890681974138
2211	3	0.13799053009157447
2211	4	0.11594256747426357
2211	5	-0.570242933277946
2211	6	0.09655575806250738
2211	7	0.2479043585646039
2211	8	0.25014812615420823
2211	9	0.8497934280802824
2211	10	0.10719725799143731
2211	11	0.5371359729728695
2211	12	0.4766291958766402
2211	13	0.42495577774779036
2211	14	0.41773756905576676
2211	15	0.35606429297644837
2211	16	0.38072892057974883
2211	17	0.30297406027585794
2211	18	0.18223865165062136
2211	19	0.12296130526711835
2211	20	0.5411235744223433
2211	21	0.6414953832519856
2211	22	0.5283764405371869
2211	23	0.9159111936207793
2211	24	0.9478418669440671
2211	25	0.9550778801168818
2212	1	0.08172687645516064
2212	2	0.16517846667986547
2212	3	0.13264388229980284
2212	4	0.14829118243142858
2212	5	-0.08397313408293172
2212	6	0.1827464282615594
2212	7	0.22630968810582885
2212	8	0.04947567239187123
2212	9	0.5727622067324347
2212	10	0.19723226668563854
2212	11	0.3387441859775677
2212	12	0.3072748606565341
2212	13	0.04635644303071018
2212	14	0.05710128345218379
2212	15	0.22394125630668782
2212	16	0.27933989988847685
2212	17	0.038497521059909445
2212	18	0.1385272841174796
2212	19	0.20487676210685635
2212	20	0.44479588405431514
2212	21	0.6473496981368712
2212	22	0.22621980080101878
2212	23	0.8060922413343476
2212	24	0.8011695123605449
2212	25	0.6864322631426063
2213	1	0.09040886860329067
2213	2	0.11741438229691425
2213	3	-0.025231777602790828
2213	4	-0.027343169859641137
2213	5	0.080286048166478
2213	6	0.15909777485050824
2213	7	0.10636159926831823
2213	8	0.22791227592873753
2213	9	0.6599761314700601
2213	10	0.29521943162428244
2213	11	0.49484330704722623
2213	12	0.3691286142600063
2213	13	0.09611133635380043
2213	14	0.30271403252356066
2213	15	0.22847846106855485
2213	16	0.0687026930120993
2213	17	-0.03131823484132923
2213	18	-0.12805591823385498
2213	19	-0.15128702765470337
2213	20	0.34327150025708164
2213	21	0.040098522823043936
2213	22	0.07474175996282639
2213	23	0.06279029669215674
2213	24	0.05943212716069013
2213	25	0.3923088322834829
2214	1	-1.1411232843557997
2214	2	-0.9706389770397266
2214	3	-1.036846807537728
2214	4	-0.8988266180285436
2214	5	-1.0795582918724733
2214	6	-1.120331306296439
2214	7	-1.1511636935473852
2214	8	-1.206302753492075
2214	9	-11.115003025039062
2214	10	-4.287377774448224
2214	11	-2.1137343571279317
2214	12	-1.5703631387439856
2214	13	-1.1960592229543892
2214	14	-1.4212525116385066
2214	15	-1.344267507119092
2214	16	-1.5431408655566088
2214	17	-1.5284262808144007
2214	18	-1.8517430353712712
2214	19	-2.054412480191953
2214	20	-1.569213857886787
2214	21	-2.2263817163479835
2214	22	-1.8888097652323061
2214	23	-2.2870520811024306
2214	24	-2.691643636893563
2214	25	-9.825496178894454
2215	1	-0.6965292744606977
2215	2	-0.33561273913512224
2215	3	-0.38293087732425235
2215	4	-0.4390875158436387
2215	5	-0.5482453873762836
2215	6	-0.5672887521556405
2215	7	-0.6050351266542471
2215	8	-0.6703787761453661
2215	9	0.2759181553343808
2215	10	0.15719792423415413
2215	11	0.03069322705486144
2215	12	0.02549094478970204
2215	13	-0.03477870351428436
2215	14	-0.2747585973657607
2215	15	-0.6869866121701733
2215	16	-0.494457884487737
2215	17	-0.5732155071501879
2215	18	-0.41499035099477055
2215	19	-0.4095251814078935
2215	20	-0.06499355319336786
2215	21	-0.3773707324572939
2215	22	-0.3085630246496649
2215	23	0.054283366817770684
2215	24	0.3178254952589725
2215	25	0.34656379901001344
2216	1	-0.5682183495140478
2216	2	-0.4827693505223394
2216	3	-0.5117344061170712
2216	4	-0.3876101663790834
2216	5	-1.2410093586775268
2216	6	-1.6219973188088197
2216	7	-1.0591690053228646
2216	8	-1.9392265254161174
2216	9	-0.41163048620118303
2216	10	-0.8205034591492719
2216	11	-0.5145028870351638
2216	12	-1.7163786988654586
2216	13	-1.3408722543480316
2216	14	-1.5460595467957248
2216	15	-1.0369108485434495
2216	16	-2.0459612256125155
2216	17	-2.3884680239680307
2216	18	-1.864035640183758
2216	19	-3.427228720036286
2216	20	-2.025398353085638
2216	21	-2.3549716147545148
2216	22	-2.2961526257412146
2216	23	-1.958127939735956
2216	24	-1.9966682301067067
2216	25	-2.1263491779410764
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	61599.0
3	2.0
4	915.0
5	9669.0
6	12321.0
7	9637.0
8	7579.0
9	6414.0
10	5754.0
11	5161.0
12	4810.0
13	4449.0
14	4573.0
15	4698.0
16	4956.0
17	5152.0
18	5875.0
19	7081.0
20	8961.0
21	11881.0
22	14738.0
23	17680.0
24	20894.0
25	23804.0
26	27347.0
27	30562.0
28	34310.0
29	40293.0
30	48957.0
31	61050.0
32	75392.0
33	92740.0
34	115855.0
35	143317.0
36	186346.0
37	264570.0
38	627879.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.12991867001555	17.426095363393536	11.156659889167962	26.287326077422957
2	17.842965324830608	19.864039457951375	35.434386209645275	26.85860900757274
3	20.368679252239044	22.75820426171733	28.0043184126894	28.868798073354228
4	13.241879457853296	14.918600271432073	34.12192122889775	37.71759904181688
5	14.179241714522368	37.154712584217805	33.4635566566146	15.20248904464522
6	35.32160671569172	34.22668947349802	16.375029210249522	14.076674600560738
7	31.410472017631584	30.30416278621304	19.261699785684353	19.023665410471022
8	28.803252309641163	33.851498258082806	18.44314313521174	18.90210629706428
9	27.291166745159018	14.478524244168517	18.035947003959503	40.194362006712964
10	15.670929176680026	29.434095229171795	31.428975831378835	23.46599976276934
11	37.62802640720646	21.350643085053893	20.664097582662073	20.357232925077575
12	23.82189431601562	24.956628491767635	28.46022924868889	22.761247943527856
13	29.953830494592133	19.575979900048278	24.91095667579561	25.55923292956398
14	22.27325154138382	19.521953042286853	26.286603973344956	31.918191442984366
15	25.023852763059235	27.792517083062425	22.122758899011263	25.060871254867084
16	24.895931267553426	27.399410189991762	23.636394436314916	24.068264106139896
17	24.45255474452555	27.083929149747142	24.957873495926858	23.505642609800454
18	23.690815190176163	26.292517769456715	26.45480342110311	23.56186361926401
19	24.941674996437214	25.35665204015431	25.876569244062107	23.82510371934636
20	24.39863929918028	25.551856663014284	26.19119869530576	23.858305342499676
21	25.008383229952617	24.781126696140426	25.933303869539504	24.277186204367453
22	24.926257802721686	25.03966134394158	25.872997998995512	24.161082854341224
23	24.037192932107253	24.976881469700775	26.34650491667364	24.639420681518327
24	24.348761895271785	25.09137561656121	26.054855263813465	24.505007224353545
25	24.109833909478894	25.154676504782316	26.405455186581882	24.330034399156904
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	268.0
1	268.0
2	368.5
3	469.0
4	469.0
5	469.0
6	1363.0
7	2257.0
8	2257.0
9	2257.0
10	2750.0
11	3243.0
12	3243.0
13	3243.0
14	3736.5
15	4230.0
16	4230.0
17	4230.0
18	7378.0
19	10526.0
20	10526.0
21	10526.0
22	17798.0
23	25070.0
24	25070.0
25	25070.0
26	38515.5
27	51961.0
28	51961.0
29	51961.0
30	67571.5
31	83182.0
32	83182.0
33	83182.0
34	105354.5
35	127527.0
36	127527.0
37	127527.0
38	150466.5
39	173406.0
40	173406.0
41	173406.0
42	198076.0
43	222746.0
44	222746.0
45	222746.0
46	246049.5
47	269353.0
48	269353.0
49	269353.0
50	274491.5
51	279630.0
52	279630.0
53	279630.0
54	268565.5
55	257501.0
56	257501.0
57	257501.0
58	230202.5
59	202904.0
60	202904.0
61	202904.0
62	171462.5
63	140021.0
64	140021.0
65	140021.0
66	109912.0
67	79803.0
68	79803.0
69	79803.0
70	60161.5
71	40520.0
72	40520.0
73	40520.0
74	29059.5
75	17599.0
76	17599.0
77	17599.0
78	13543.5
79	9488.0
80	9488.0
81	9488.0
82	6560.5
83	3633.0
84	3633.0
85	3633.0
86	2468.0
87	1303.0
88	1303.0
89	1303.0
90	921.0
91	539.0
92	539.0
93	539.0
94	290.5
95	42.0
96	42.0
97	42.0
98	21.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.028945492300050668
2	0.0010462226132548435
3	9.964024888141365E-5
4	0.004433991075222908
5	0.033279843126392164
6	0.010960427376955502
7	0.018333805794180112
8	0.02610574520693038
9	0.03268200163310368
10	0.03661779146391952
11	0.03980627942812476
12	0.007124277795021077
13	0.002590646470916755
14	0.004782731946307856
15	0.005779134435121992
16	0.006675896675054716
17	0.008519241279360868
18	0.013252153101228015
19	0.01808470517197658
20	0.015643519074381943
21	0.011857189616888225
22	0.011408808496921864
23	0.008618881528242282
24	0.006028235057325526
25	0.11109887750277622
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	2007221.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	48.96475261248511
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.4398760048283	37.42859618305455
2	12.283161303057696	12.028839090069408
3	4.423090043999422	6.4972652936153255
4	2.027564891231752	3.9711685321969203
5	1.1515035844156414	2.819154407165087
6	0.7339057843674917	2.1561309103455644
7	0.46995007498097086	1.6107692413163444
8	0.36488772308712647	1.4293309673835295
9	0.2611090000634991	1.1506623833702332
>10	1.60088616898785	14.814574957188018
>50	0.1472195428735227	4.997256966181342
>100	0.0909279857943605	8.09874961166712
>500	0.004387402921215763	1.4302555367704226
>1k	0.0014284567650469926	1.3120001624677964
>5k	1.020326260747852E-4	0.25524575720833065
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	5109	0.2545310157675712	No Hit
GGTATCAACGCAGAGTACTTTTTTT	3788	0.18871863138139747	No Hit
TATCAACGCAGAGTACTTTTTTTTT	3761	0.18737348802149836	No Hit
GTGGTATCAACGCAGAGTACATGGG	2415	0.12031560052430698	No Hit
ACGCAGAGTACTTTTTTTTTTTTTT	2274	0.11329096297816733	No Hit
TGCATGCACTGCCTCAGTGACCAGT	2220	0.11060067625836915	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	4.982012444070683E-5	0.0	0.0	0.0	0.0
6	4.982012444070683E-5	0.0	0.0	0.0	0.0
7	4.982012444070683E-5	0.0	0.0	0.0	0.0
8	9.964024888141366E-5	0.0	0.0	0.0	4.982012444070683E-5
9	9.964024888141366E-5	0.0	0.0	0.0	4.982012444070683E-5
10	1.4946037332212047E-4	0.0	0.0	4.982012444070683E-5	4.982012444070683E-5
11	1.4946037332212047E-4	0.0	0.0	4.982012444070683E-5	4.982012444070683E-5
12	1.4946037332212047E-4	0.0	0.0	4.982012444070683E-5	4.982012444070683E-5
13	1.4946037332212047E-4	0.0	0.0	4.982012444070683E-5	4.982012444070683E-5
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGTCGG	25	0.006033591	18.999502	10
GTCGGCG	35	0.0021755798	16.28001	12
TCGGCGT	45	6.77512E-4	14.774444	13
GGGCGTA	90	5.4043994E-7	12.667282	9
CGCGGTT	120	7.4396667E-10	12.662862	18
GGTATCA	1915	0.0	12.403928	1
TAGACCG	55	0.0030598547	12.095717	5
CTCGCGG	55	0.0030741708	12.0881815	14
ACGCTAC	65	8.0152345E-4	11.69404	1
CCTACGC	65	8.018588E-4	11.693458	3
CTTCGCG	115	7.099152E-8	11.562609	15
TTCGCGG	135	4.7475623E-9	11.256719	16
GTGGTAT	710	0.0	11.241103	1
TAATACC	60	0.0058658286	11.086082	4
TAGACAG	370	0.0	11.04493	5
TGGACGC	175	2.0008883E-11	10.861461	5
TTGACCG	210	0.0	10.853882	18
TAGGCGG	115	8.774423E-7	10.743402	5
TTATACC	115	8.7913577E-7	10.741794	4
GGTCTAG	80	3.7665403E-4	10.689085	1
>>END_MODULE