FastQCFastQC Report
Thu 26 May 2016
SRR1364310_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364310_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1442888
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT50580.3505469585996973No Hit
GTACTTTTTTTTTTTTTTTTTTTTT44670.3095874385260672No Hit
NNNNNNNNNNNNNNNNNNNNNNNNN40040.27749901586263104No Hit
TATCAACGCAGAGTACTTTTTTTTT32790.2272525656877041No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA23030.15961044793497486No Hit
GGTATCAACGCAGAGTACTTTTTTT22030.1526799030832608No Hit
GAGTACTTTTTTTTTTTTTTTTTTT17150.11885884420689617No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAACGC1650.014.3827643
TATCACG655.4918E-513.1436342
TAACGCA1750.013.0352354
GGTATCA15000.012.466981
AGGACCG550.00305731912.0966725
CGACAGT550.003070104612.0899346
TCACGCA802.8510452E-511.8810744
TATAACG1950.011.1964292
GAACAAA5800.011.1292841
GACACCG600.005878817811.0824397
GGCTAAT600.00590935511.0747291
TTTATAC1855.456968E-1210.7754123
GGACCAT1605.4387783E-1010.6866396
AACTCAG6550.010.4419067
TAATACC1553.6288839E-910.42468554
ATCAACT5400.010.3849394
TGCACTC1106.0087514E-610.3685765
GTTCTAA1659.404175E-1010.355591
GTATCAA32300.010.2273471
GTAGGAC750.002664473610.1254663
Produced by FastQC (version 0.11.5)