FastQCFastQC Report
Thu 26 May 2016
SRR1364310_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364310_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1442888
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACTTTTTTTTTTTTTTTTTTTTT114970.7968047416015657No Hit
GTATCAACGCAGAGTACTTTTTTTT112560.7801021285089349No Hit
TATCAACGCAGAGTACTTTTTTTTT87170.6041355947239149No Hit
GGTATCAACGCAGAGTACTTTTTTT78250.5423151346466254No Hit
GAGTACTTTTTTTTTTTTTTTTTTT52630.3647545755457111No Hit
ACGCAGAGTACTTTTTTTTTTTTTT37050.256776686756006No Hit
GTATAACGCAGAGTACTTTTTTTTT16690.11567079357510769No Hit
GTGGTATCAACGCAGAGTACTTTTT16250.11262135384035352No Hit
GTGGTATCAACGCAGAGTACATGGG15580.1079778887897051No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAACGCA2550.014.5302334
TATAACG2600.014.2483372
ATAACGC2600.013.88443853
AGGACCG707.2262956E-613.57784755
ATAGGAC1205.0931703E-1113.4581613
TTGACCG500.001504702313.29383818
GGTATCA23150.013.1758161
GTATAAC3400.013.1353741
GAATCGA751.4738642E-512.6704596
TATCACG1207.421477E-1012.6651882
CTGGTCG701.08775086E-412.2179429
TCGAACC951.0358362E-612.0035939
TCACGCA1201.0000804E-811.8756714
GGTCGGT658.0189965E-411.69296911
CGAGGTT658.0189965E-411.6929694
TGGTCGG752.0691921E-411.40262210
AATCGAA855.305803E-511.1798177
TGTACCG600.00585467111.0885745
ACCGAGT700.001487569210.8603948
TAGCCCC700.001490585310.8577584
Produced by FastQC (version 0.11.5)