##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1364310_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1442888 Sequences flagged as poor quality 0 Sequence length 25 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.599178175991483 31.0 30.0 34.0 26.0 34.0 2 29.61615108033333 31.0 30.0 34.0 26.0 34.0 3 29.674222115645843 31.0 30.0 34.0 26.0 34.0 4 33.03806047316216 35.0 33.0 37.0 27.0 37.0 5 32.60133704071279 35.0 33.0 37.0 25.0 37.0 6 32.48654365411591 35.0 33.0 37.0 25.0 37.0 7 32.216661307045314 35.0 32.0 37.0 22.0 37.0 8 32.10167663741053 35.0 32.0 37.0 19.0 37.0 9 32.81946762326667 37.0 32.0 39.0 16.0 39.0 10 32.96865106647224 37.0 32.0 39.0 17.0 39.0 11 32.91602882552215 37.0 32.0 39.0 17.0 39.0 12 32.793955594613024 37.0 32.0 39.0 17.0 39.0 13 32.896185982557206 37.0 32.0 39.0 17.0 39.0 14 33.82934919411625 38.0 33.0 41.0 17.0 41.0 15 33.86000299399537 38.0 33.0 41.0 17.0 41.0 16 33.81521088261875 38.0 33.0 41.0 17.0 41.0 17 33.77947837947228 38.0 33.0 41.0 17.0 41.0 18 33.73476458325248 38.0 33.0 41.0 16.0 41.0 19 33.64468967792372 39.0 32.0 41.0 11.0 41.0 20 33.48409647872877 39.0 32.0 41.0 10.0 41.0 21 33.41098200276113 39.0 32.0 41.0 10.0 41.0 22 33.38537710480647 39.0 32.0 41.0 2.0 41.0 23 33.08293713718598 38.0 32.0 41.0 2.0 41.0 24 32.99385676504344 38.0 32.0 41.0 2.0 41.0 25 32.85347303463609 38.0 32.0 41.0 2.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.36360253626488515 1101 2 0.6059719102732082 1101 3 -1.3922373145935225 1101 4 -0.2688828463427555 1101 5 0.5233536427803145 1101 6 0.9324855278680673 1101 7 1.0708733399460542 1101 8 1.1955431864107915 1101 9 2.0332168422942374 1101 10 1.6557373743877406 1101 11 1.6441698800064017 1101 12 1.7331980756690584 1101 13 1.494004746715163 1101 14 1.6726735891578173 1101 15 1.50892728142977 1101 16 1.5551633209153906 1101 17 1.6643284054696181 1101 18 1.7176258626722998 1101 19 1.6769739221537279 1101 20 1.8129181049979124 1101 21 1.6211579048630327 1101 22 1.8109136441320643 1101 23 2.234087550943663 1101 24 2.136473104943228 1101 25 2.168599347315606 1102 1 0.32071087081210337 1102 2 0.3560586551630358 1102 3 0.4956612822669655 1102 4 0.43311981492499996 1102 5 0.5869360103265961 1102 6 0.711262326719762 1102 7 0.7908407860619278 1102 8 0.9066200996675384 1102 9 1.485907325070471 1102 10 1.07422050086997 1102 11 1.04671772472323 1102 12 1.2469197260478708 1102 13 0.9594635044301398 1102 14 1.039735408803736 1102 15 1.183616759274635 1102 16 1.014168176992527 1102 17 0.9980544094598827 1102 18 1.0686783400572537 1102 19 1.0603462361309468 1102 20 1.0921269916026972 1102 21 1.350260569895184 1102 22 1.4144612646215364 1102 23 1.5568468731551022 1102 24 1.5735671020331878 1102 25 1.3840130958896353 1103 1 -2.288555295975094 1103 2 -2.265184319246689 1103 3 -2.4281070984534168 1103 4 -2.275465678420513 1103 5 -4.960051391188266 1103 6 -8.925722410462896 1103 7 -9.534451973298125 1103 8 -11.149778103811322 1103 9 -12.268422214761298 1103 10 -10.12430713840482 1103 11 -12.530769936881203 1103 12 -11.556715818681191 1103 13 -12.089119324583173 1103 14 -12.289626800815771 1103 15 -11.846902531759252 1103 16 -6.773884196166229 1103 17 -4.891302852114105 1103 18 -3.8454350082470903 1103 19 -3.3813112322459666 1103 20 -8.660293110149418 1103 21 -5.102165241140224 1103 22 -10.68680556190506 1103 23 -11.85821485054096 1103 24 -12.432995401931596 1103 25 -12.608736114326177 1104 1 0.19201658995262605 1104 2 0.5337472456423242 1104 3 0.4837057157805269 1104 4 0.5958091704772244 1104 5 0.5355703751598426 1104 6 0.7408886747101846 1104 7 0.6562814800912378 1104 8 0.9287585466255486 1104 9 1.3587085414963838 1104 10 1.1152109859262183 1104 11 1.1305609064012145 1104 12 1.1163331914918118 1104 13 0.9710579645009432 1104 14 1.086289896817732 1104 15 1.2407272510282752 1104 16 0.8059625894765929 1104 17 0.8354966384418034 1104 18 0.9218516302794626 1104 19 1.060729974285337 1104 20 1.2121981794365198 1104 21 1.2010222781147917 1104 22 0.9593420053723634 1104 23 1.2984979646458115 1104 24 1.4284129378333077 1104 25 1.6186509520190668 1105 1 0.462252096364562 1105 2 0.5127691299655233 1105 3 -0.025892352978832633 1105 4 0.3825647020259737 1105 5 0.6430779169126524 1105 6 0.6915543395298727 1105 7 0.7398429040585768 1105 8 0.528976150061748 1105 9 0.7154781409733602 1105 10 0.3345166556482724 1105 11 1.1470700757157246 1105 12 1.2524487672430311 1105 13 0.9283235634552085 1105 14 0.7121004564282671 1105 15 0.620152647281003 1105 16 0.9929071543721903 1105 17 0.883154307085988 1105 18 0.9683827283550173 1105 19 0.927564138511805 1105 20 1.1459632566011209 1105 21 0.927934023931904 1105 22 0.6739148409395312 1105 23 1.3283466060751579 1105 24 1.2937380413034987 1105 25 1.536020896731479 1106 1 -0.17073385741885616 1106 2 0.10044470767281766 1106 3 -0.016377174002847994 1106 4 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0.21140282273971422 1107 16 0.09700576854714882 1107 17 -0.06840491065882759 1107 18 0.04645572687498856 1107 19 0.19462475091283693 1107 20 0.056645101804917886 1107 21 0.08312439131459115 1107 22 0.26892130126337577 1107 23 0.37921211122711185 1107 24 0.5229232708095708 1107 25 0.3062383480521973 1108 1 -0.12270485877816739 1108 2 -0.03911125064163912 1108 3 0.010355947882064953 1108 4 -0.1634253296912931 1108 5 0.13016445973095614 1108 6 0.23437264492180532 1108 7 -9.81744786010097E-4 1108 8 0.1991166122275878 1108 9 0.49572281700416454 1108 10 0.21172553648198544 1108 11 0.30610949574290913 1108 12 0.37977092401693113 1108 13 -0.047661937224447115 1108 14 -0.06905042712406839 1108 15 -0.21627689508419934 1108 16 -0.9422990078389546 1108 17 -0.992695262465439 1108 18 -0.7801446844225097 1108 19 -0.7271707957881475 1108 20 -0.04796515300467519 1108 21 -0.258291621740959 1108 22 -0.060566355254394466 1108 23 -0.602781622289136 1108 24 -0.26811062204040326 1108 25 -0.3330321164085248 1109 1 0.24796184846694658 1109 2 0.2159757550913355 1109 3 0.21738952781623766 1109 4 0.43286883015562694 1109 5 0.2755330805635978 1109 6 0.26963585055382566 1109 7 0.4657141276850041 1109 8 0.27607817809652246 1109 9 0.935007613841293 1109 10 0.36419585297986856 1109 11 0.29196897431118174 1109 12 0.4746694321783025 1109 13 0.5153566268150556 1109 14 0.22948108020577962 1109 15 -0.11013028127896973 1109 16 0.3108132023162966 1109 17 0.2993904784613264 1109 18 0.31780621739012815 1109 19 0.4122189777620875 1109 20 0.6170626248787769 1109 21 0.6181536100677647 1109 22 0.2821288532784507 1109 23 0.7362715370133728 1109 24 0.21968430836322028 1109 25 0.2693347951237115 1110 1 0.031678989846124495 1110 2 -0.08993777645958545 1110 3 -0.08782191347615509 1110 4 0.03765769589412571 1110 5 -0.05853751555436304 1110 6 0.1831306743627934 1110 7 0.06655103948280328 1110 8 0.09930028463157825 1110 9 -3.303256324681019 1110 10 -1.4051413606020944 1110 11 -0.4375140750574431 1110 12 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-0.5873345671655308 2215 2 -0.48404485143366216 2215 3 -0.38205315421152264 2215 4 -0.5076853007745399 2215 5 -0.5319982087682114 2215 6 -0.5196441604765027 2215 7 -0.5862830665124683 2215 8 -0.5585499381694881 2215 9 0.14416779811711677 2215 10 0.029535527246082438 2215 11 0.1530830677804218 2215 12 0.14887631641521892 2215 13 -0.03896605328390024 2215 14 -0.11445312357429316 2215 15 -0.788967794497097 2215 16 -0.466289557940911 2215 17 -0.41313267562537703 2215 18 -0.3764701790221494 2215 19 -0.560321226622996 2215 20 -0.2957794971186374 2215 21 -0.3058346830150356 2215 22 -0.3843532211167542 2215 23 -0.15781035941981258 2215 24 0.28331665480599355 2215 25 0.3643915271096745 2216 1 -0.43053231864004715 2216 2 -0.5106056282374674 2216 3 -0.6098981967607777 2216 4 -0.3113923499464377 2216 5 -1.187386436830554 2216 6 -1.5269944940420856 2216 7 -1.1072395659999863 2216 8 -1.9034659313771805 2216 9 -0.5881091398940583 2216 10 -1.2036286938292555 2216 11 -0.7550650556437155 2216 12 -1.9226640097656151 2216 13 -1.4773680258893869 2216 14 -1.7203968770597982 2216 15 -1.2393018468356658 2216 16 -2.352690901938331 2216 17 -2.6726681871134694 2216 18 -2.387597723876535 2216 19 -3.5851251690525103 2216 20 -2.426455918336103 2216 21 -2.4560462819463993 2216 22 -2.3754025098300424 2216 23 -2.0294891323546196 2216 24 -2.1126866078367463 2216 25 -2.200055402098208 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 52892.0 3 2.0 4 774.0 5 7650.0 6 10362.0 7 8162.0 8 6436.0 9 5143.0 10 4440.0 11 3932.0 12 3564.0 13 3317.0 14 3323.0 15 3362.0 16 3523.0 17 3743.0 18 4200.0 19 5198.0 20 7230.0 21 9256.0 22 11304.0 23 13454.0 24 15764.0 25 17488.0 26 19771.0 27 21489.0 28 23827.0 29 27820.0 30 34126.0 31 42161.0 32 52020.0 33 64324.0 34 80030.0 35 99918.0 36 130804.0 37 193723.0 38 448356.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.736340412551954 18.55222064700846 11.974127980533593 24.737310959905994 2 16.263835272754996 21.60035207607061 38.69842743975549 23.437385211418906 3 18.698276441606307 27.259237902898843 29.591991611262696 24.450494044232155 4 11.850481591939113 17.121135776559637 37.22046909556916 33.80791353593209 5 11.991258915794962 39.20731233846188 34.68195709325672 14.11947165248644 6 29.83746092477456 37.81109424493842 18.843858518225865 13.507586312061163 7 26.86482533454872 32.765706720088176 22.67278518523653 17.696682760126574 8 26.29644803913209 35.310527078354994 20.49505753556848 17.897967346944434 9 26.69068678496009 15.351268231741223 19.96932893881179 37.98871604448691 10 14.591864042963575 30.49569518379187 33.3792079249108 21.533232848333746 11 34.094295315740176 23.252129697484854 23.5904685507796 19.063106435995373 12 22.611066097297762 26.783396071576355 30.802964026762464 19.802573804363423 13 28.075087760639573 22.331574077603626 26.906978920119624 22.686359241637184 14 21.521837821727967 21.457656839675767 29.361967569890385 27.658537768705884 15 23.309426016465277 29.311946126751288 25.12895118776485 22.249676669018584 16 22.031030694534454 28.508384941414576 27.118068431102778 22.342515932948192 17 21.30528956953585 27.978055720172314 28.28607966064689 22.430575049644947 18 20.769664257032566 27.050539932251134 30.513814211180435 21.665981599535865 19 22.256880975602318 26.49398289382568 30.23518736868665 21.013948761885352 20 22.142077336041286 26.15423539075253 30.279586737045676 21.42410053616051 21 22.980118817151755 25.5409049982637 30.1125442826437 21.366431901940846 22 22.12406003311761 26.057763819739844 30.170918192297002 21.647257954845546 23 21.59697303861261 25.752945555311424 30.42369280694665 22.22638859912931 24 21.401609927377223 26.21954008807886 30.588065689029232 21.790784295514687 25 21.703682683775142 25.728623831187257 30.510929964553153 22.056763520484445 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 999.0 1 999.0 2 1631.5 3 2264.0 4 2264.0 5 2264.0 6 8062.0 7 13860.0 8 13860.0 9 13860.0 10 12053.0 11 10246.0 12 10246.0 13 10246.0 14 9906.5 15 9567.0 16 9567.0 17 9567.0 18 15208.0 19 20849.0 20 20849.0 21 20849.0 22 30555.5 23 40262.0 24 40262.0 25 40262.0 26 57912.5 27 75563.0 28 75563.0 29 75563.0 30 88833.0 31 102103.0 32 102103.0 33 102103.0 34 114100.5 35 126098.0 36 126098.0 37 126098.0 38 135941.0 39 145784.0 40 145784.0 41 145784.0 42 154681.5 43 163579.0 44 163579.0 45 163579.0 46 172124.0 47 180669.0 48 180669.0 49 180669.0 50 176812.0 51 172955.0 52 172955.0 53 172955.0 54 158572.5 55 144190.0 56 144190.0 57 144190.0 58 124504.0 59 104818.0 60 104818.0 61 104818.0 62 86116.5 63 67415.0 64 67415.0 65 67415.0 66 50738.0 67 34061.0 68 34061.0 69 34061.0 70 25049.5 71 16038.0 72 16038.0 73 16038.0 74 11312.0 75 6586.0 76 6586.0 77 6586.0 78 4798.5 79 3011.0 80 3011.0 81 3011.0 82 2110.0 83 1209.0 84 1209.0 85 1209.0 86 832.5 87 456.0 88 456.0 89 456.0 90 368.0 91 280.0 92 280.0 93 280.0 94 152.5 95 25.0 96 25.0 97 25.0 98 13.0 99 1.0 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.02793009575240767 2 0.0012474980733085312 3 6.930544851714062E-5 4 0.0047820759476827025 5 0.034929946052638876 6 0.010950260865708219 7 0.01892038744517939 8 0.026959819473167702 9 0.03368244797933034 10 0.03811799668442734 11 0.03791008033887592 12 0.008385959270574016 13 0.002980134286237047 14 0.006445406712094078 15 0.0065147121606112185 16 0.008524570167608296 17 0.00991067913795111 18 0.014346227843048108 19 0.017395667577802296 20 0.015455115019322358 21 0.011574009902362485 22 0.010465122726088233 23 0.008385959270574016 24 0.006237490366542656 25 0.10915608141449648 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 1442888.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 59.87708833885827 #Duplication Level Percentage of deduplicated Percentage of total 1 81.21101197603261 48.62678938176982 2 11.499641697408917 13.771301235619438 3 3.1951380501327646 5.739466898479408 4 1.3052802797392142 3.126255304676583 5 0.6680516615543014 2.000049417690398 6 0.4159164389972449 1.4942319215652837 7 0.26565767313235494 1.1134765573429088 8 0.20662007058258022 0.989744657508343 9 0.15440784085657608 0.8320942734463419 >10 0.9589897511957256 10.76501833927214 >50 0.07842037244472914 3.1836624916056073 >100 0.03807804029884825 3.8696535624854667 >500 0.0015091632964183176 0.6002970226879116 >1k 6.965369060392236E-4 0.790687660152269 >5k 3.482684530196118E-4 1.5156874223841956 >10k+ 2.3217896867974117E-4 1.5815838533138087 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTACTTTTTTTTTTTTTTTTTTTTT 11497 0.7968047416015657 No Hit GTATCAACGCAGAGTACTTTTTTTT 11256 0.7801021285089349 No Hit TATCAACGCAGAGTACTTTTTTTTT 8717 0.6041355947239149 No Hit GGTATCAACGCAGAGTACTTTTTTT 7825 0.5423151346466254 No Hit GAGTACTTTTTTTTTTTTTTTTTTT 5263 0.3647545755457111 No Hit ACGCAGAGTACTTTTTTTTTTTTTT 3705 0.256776686756006 No Hit GTATAACGCAGAGTACTTTTTTTTT 1669 0.11567079357510769 No Hit GTGGTATCAACGCAGAGTACTTTTT 1625 0.11262135384035352 No Hit GTGGTATCAACGCAGAGTACATGGG 1558 0.1079778887897051 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 0.0 0.0 12 0.0 0.0 0.0 0.0 0.0 13 0.0 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TAACGCA 255 0.0 14.530233 4 TATAACG 260 0.0 14.248337 2 ATAACGC 260 0.0 13.8844385 3 AGGACCG 70 7.2262956E-6 13.5778475 5 ATAGGAC 120 5.0931703E-11 13.458161 3 TTGACCG 50 0.0015047023 13.293838 18 GGTATCA 2315 0.0 13.175816 1 GTATAAC 340 0.0 13.135374 1 GAATCGA 75 1.4738642E-5 12.670459 6 TATCACG 120 7.421477E-10 12.665188 2 CTGGTCG 70 1.08775086E-4 12.217942 9 TCGAACC 95 1.0358362E-6 12.003593 9 TCACGCA 120 1.0000804E-8 11.875671 4 GGTCGGT 65 8.0189965E-4 11.692969 11 CGAGGTT 65 8.0189965E-4 11.692969 4 TGGTCGG 75 2.0691921E-4 11.402622 10 AATCGAA 85 5.305803E-5 11.179817 7 TGTACCG 60 0.005854671 11.088574 5 ACCGAGT 70 0.0014875692 10.860394 8 TAGCCCC 70 0.0014905853 10.857758 4 >>END_MODULE