FastQCFastQC Report
Thu 26 May 2016
SRR1364309_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364309_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5794442
Sequences flagged as poor quality0
Sequence length25
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNN167290.28870769609912394No Hit
GTATCAACGCAGAGTACTTTTTTTT83930.14484569868850183No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCATAT2250.012.6671058
GGTATCA30300.012.627981
GTATCAA80050.011.3388241
GGACCGA5300.011.1138826
AGGACCG6300.010.8628865
GTATTAG6950.09.9726261
TCGTTCG4100.09.97015514
ACCGAGT4950.09.7882188
GTCTAGG5600.09.6640311
GTCCTAC5150.09.5866661
ACGCATA6750.09.57078557
TAACGCA1502.6316593E-79.505194
AACGCAT7200.09.5005766
ACCGTTT1106.840742E-59.5003298
TGGTATC11800.09.4946512
CGCAGAT10550.09.4553058
CGCGCCA2650.09.32099610
TGCCGTA1752.6471753E-89.23449315
CGAGTGG5050.09.21806110
GTATTAC4850.09.2008431
Produced by FastQC (version 0.11.5)