##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1364309_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	5794442
Sequences flagged as poor quality	0
Sequence length	25
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	26.12185763529948	30.0	26.0	31.0	7.0	34.0
2	26.046465388729406	30.0	26.0	31.0	7.0	34.0
3	25.375494655050478	30.0	24.0	31.0	7.0	34.0
4	29.28077630253267	35.0	28.0	37.0	7.0	37.0
5	29.73387670460762	35.0	30.0	37.0	2.0	37.0
6	29.98241780658086	35.0	30.0	37.0	2.0	37.0
7	29.590066998685984	35.0	30.0	37.0	2.0	37.0
8	29.110220794340506	35.0	28.0	37.0	2.0	37.0
9	30.26959127384483	35.0	28.0	39.0	2.0	39.0
10	29.512517685050604	35.0	27.0	39.0	2.0	39.0
11	30.192572468582824	35.0	28.0	39.0	2.0	39.0
12	29.68444485249831	35.0	27.0	39.0	2.0	39.0
13	29.325978066567927	35.0	27.0	39.0	2.0	39.0
14	29.60652328558988	36.0	26.0	40.0	2.0	41.0
15	30.303885516500124	36.0	27.0	40.0	2.0	41.0
16	30.660983577020875	36.0	28.0	40.0	2.0	41.0
17	30.820218064138015	37.0	29.0	40.0	2.0	41.0
18	30.650186333731533	37.0	28.0	40.0	2.0	41.0
19	30.61686888918726	37.0	28.0	40.0	2.0	41.0
20	30.46395666744097	37.0	27.0	40.0	2.0	41.0
21	30.383971226219884	37.0	27.0	40.0	2.0	41.0
22	30.43935654201043	37.0	28.0	40.0	2.0	41.0
23	30.43984079916582	37.0	29.0	40.0	2.0	41.0
24	30.276068170153398	37.0	28.0	40.0	2.0	41.0
25	30.078595661152534	37.0	28.0	40.0	2.0	41.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1101	1	0.5295423483431101
1101	2	0.7504571333973793
1101	3	1.5278928215620091
1101	4	0.8160559525341071
1101	5	0.39692686548823275
1101	6	0.25208539269412356
1101	7	0.5687524454925068
1101	8	0.8142476475449705
1101	9	0.7273960480961037
1101	10	1.0299594260756102
1101	11	0.5498163145735546
1101	12	0.8770127381671315
1101	13	0.8396078287404407
1101	14	1.3892128875146774
1101	15	0.6639335298150506
1101	16	0.09363190264213372
1101	17	-0.6112256947807602
1101	18	-6.417629120472668
1101	19	-7.7699172531992495
1101	20	-8.897965525213248
1101	21	-10.513786866062812
1101	22	-10.896494545778875
1101	23	-12.171101877638367
1101	24	-11.9151202696798
1101	25	-12.201714585852198
1102	1	1.6769604731164591
1102	2	1.9297904636582004
1102	3	2.7294529032486636
1102	4	2.0319585433558025
1102	5	1.4434166055152886
1102	6	1.4550358112330386
1102	7	1.7250624693696004
1102	8	2.0531580933185225
1102	9	2.166550931205009
1102	10	-1.4107021938258413
1102	11	0.6966283674064826
1102	12	1.7094747034264977
1102	13	2.19870858943208
1102	14	2.838882019287805
1102	15	2.3696323416431113
1102	16	2.0222203107392573
1102	17	1.6779532865945725
1102	18	1.825313209489245
1102	19	1.5147726491960753
1102	20	1.9316896372974064
1102	21	2.074075225451381
1102	22	1.9165726349265881
1102	23	1.9881592808505744
1102	24	2.1744543724611667
1102	25	2.1775611995690305
1103	1	-1.2280771478541936
1103	2	-0.6364196093708081
1103	3	-3.6177125518001496
1103	4	-1.4482284677104644
1103	5	-5.108292779545909
1103	6	-8.639980046949468
1103	7	-8.6928121077244
1103	8	-3.6481287949070484
1103	9	-7.830031474291911
1103	10	-5.438865422343877
1103	11	-9.244114656272565
1103	12	-3.642921566033749
1103	13	-1.4650603677175198
1103	14	-0.9257617599917474
1103	15	-1.4154644475561327
1103	16	-1.7279767207528174
1103	17	-1.8154504517365382
1103	18	-1.5216185047780932
1103	19	-7.237326580450553
1103	20	-3.448011197553665
1103	21	-8.556657445862282
1103	22	-4.675935350640497
1103	23	-3.470957635412365
1103	24	-8.915656387014266
1103	25	-5.96145117417003
1104	1	1.403166179983561
1104	2	1.4472285013670856
1104	3	2.3160124755781304
1104	4	1.7340439707539232
1104	5	1.3851350544391998
1104	6	1.1789883873440417
1104	7	1.3436642182256087
1104	8	1.7328476464409093
1104	9	1.8603542215117415
1104	10	2.172414979958198
1104	11	1.7178152847595456
1104	12	2.0829140576346923
1104	13	2.351144262728596
1104	14	2.7142810062120084
1104	15	1.9202920894387958
1104	16	1.0173494324680732
1104	17	0.8648724050569072
1104	18	0.7740128783145899
1104	19	1.3130358886425633
1104	20	-9.185881080160389
1104	21	-3.4565497257855213
1104	22	-1.1466582934996623
1104	23	-0.04356685198442989
1104	24	0.5400714889922469
1104	25	1.0731587853625335
1105	1	1.1675500450718168
1105	2	1.1596381253475876
1105	3	1.8973574638888877
1105	4	1.2689848973363276
1105	5	1.155656014059609
1105	6	1.0224630203332161
1105	7	1.2869533639937991
1105	8	1.5343193733599136
1105	9	1.4450903468062748
1105	10	2.057564005655614
1105	11	1.5290635260221066
1105	12	1.831119454771482
1105	13	1.8699154201564099
1105	14	2.5659207107565685
1105	15	1.9242599354997125
1105	16	1.391611409865142
1105	17	0.7191078977908596
1105	18	1.2203325281127242
1105	19	1.1082131586738768
1105	20	1.036268060276523
1105	21	1.0115800353746351
1105	22	0.9924052032374782
1105	23	0.9697992289798378
1105	24	1.072269021620201
1105	25	1.062787392780976
1106	1	1.0538116121926393
1106	2	1.3774499233007944
1106	3	2.040289499581771
1106	4	1.571785145750571
1106	5	0.7877646067163866
1106	6	0.6287015960847953
1106	7	1.1302554994008496
1106	8	1.1998980724848245
1106	9	1.4852507592519757
1106	10	-2.254140633813641
1106	11	0.03746068519919632
1106	12	0.6880524284169418
1106	13	1.6338895513179672
1106	14	0.7322805512818178
1106	15	1.320067622022325
1106	16	0.977069014015953
1106	17	-5.906370160872868
1106	18	-1.7357021170828268
1106	19	-0.2398119265522034
1106	20	0.5777216612122942
1106	21	0.8522430799038148
1106	22	0.9889207132248004
1106	23	0.5585198614148261
1106	24	0.9080556124464358
1106	25	0.9866830841421184
1107	1	0.8488544846329944
1107	2	1.2031977858021428
1107	3	1.716681671482963
1107	4	1.1565386926122514
1107	5	0.6245255088279364
1107	6	0.8916370402231735
1107	7	0.6906824979924266
1107	8	0.9458412435074344
1107	9	1.0446637337366305
1107	10	-2.1832166111395104
1107	11	-0.046729236664923235
1107	12	0.4137932684588641
1107	13	1.3221627528389348
1107	14	1.679647067639305
1107	15	1.4347789582428305
1107	16	-5.4993036031908105
1107	17	-1.7703968511273835
1107	18	0.03811216606368717
1107	19	0.8176942523480548
1107	20	1.1459246186043757
1107	21	1.2266643374594999
1107	22	0.9074172327120884
1107	23	0.6385878074664113
1107	24	0.9237727857482376
1107	25	1.154271385996978
1108	1	-0.469462896809663
1108	2	-3.2293036715775827
1108	3	-0.15586833378063147
1108	4	-0.5621977761020851
1108	5	-0.902782536585466
1108	6	-0.5386030778434154
1108	7	-0.21610272902298888
1108	8	0.08601736473711696
1108	9	-0.47001197403336903
1108	10	-3.6050450164119177
1108	11	-1.7167206978029093
1108	12	-6.196698143714709
1108	13	-2.0161850048979346
1108	14	-6.336609725421386
1108	15	-2.9071805058060534
1108	16	-7.87473486193327
1108	17	-3.558509696787816
1108	18	-2.147528290050431
1108	19	-0.8359674786930462
1108	20	-0.77879214267724
1108	21	-0.27349239297611305
1108	22	-0.39950995628681696
1108	23	-0.3825185198816179
1108	24	-0.1306659064062572
1108	25	-0.3809935570590248
1109	1	-8.41604397137706
1109	2	-6.212805492951254
1109	3	-5.448943093932204
1109	4	-5.183670322915425
1109	5	-2.4535245501999228
1109	6	-1.6405979560999526
1109	7	-1.1500514264465025
1109	8	-0.6444154117244842
1109	9	-5.72363139476176
1109	10	-1.634121977767137
1109	11	-1.252466462277365
1109	12	-6.175056262020167
1109	13	-8.067572635836282
1109	14	-8.214867770856916
1109	15	-4.133076545624469
1109	16	-2.399644879369866
1109	17	-1.9352901692950546
1109	18	-1.321363953569385
1109	19	-1.065509074787908
1109	20	-0.8044307720035064
1109	21	-0.693809108129102
1109	22	-0.9131233122197706
1109	23	-1.1894418746248334
1109	24	-0.8363134319310177
1109	25	-0.7342322988923691
1110	1	-2.5362840175844994
1110	2	-2.3879621675539937
1110	3	-1.5624549955660285
1110	4	-2.449373317488128
1110	5	-5.814670957603774
1110	6	-4.2544557756830095
1110	7	-9.207381364481158
1110	8	-10.294267571905202
1110	9	-11.687081357708983
1110	10	-9.433626634878529
1110	11	-12.189439112339208
1110	12	-6.927678302938922
1110	13	-5.102112759652723
1110	14	-4.614645054343789
1110	15	-10.277694691622312
1110	16	-11.800749689033598
1110	17	-12.744968536528063
1110	18	-9.395863693476407
1110	19	-12.591563855586266
1110	20	-13.677316374124466
1110	21	-14.558283696511971
1110	22	-15.338208275608734
1110	23	-16.030002332152375
1110	24	-16.445674460977653
1110	25	-17.04122543460835
1111	1	-0.5266741880681636
1111	2	0.13180493639035973
1111	3	-2.8394257713429916
1111	4	-3.218904273112262
1111	5	-1.01132577623056
1111	6	-0.38982273992835204
1111	7	0.05786466780316246
1111	8	0.4402661320120238
1111	9	-4.238634684956978
1111	10	-4.208885603215929
1111	11	-1.2933122156166235
1111	12	-5.9709048437728605
1111	13	-1.2397976159219404
1111	14	0.24493412475731446
1111	15	0.40477801620233933
1111	16	0.18783621643830983
1111	17	0.17950647355136695
1111	18	0.5111593375493939
1111	19	0.5296422164694086
1111	20	0.5744126955159139
1111	21	0.7867718133564487
1111	22	0.38865223874362087
1111	23	0.25044683596497563
1111	24	0.4152733657277494
1111	25	0.6997305969412047
1112	1	-0.08587277414623884
1112	2	0.5082943169660297
1112	3	-2.54753078233437
1112	4	-0.32988367633464577
1112	5	-0.2719991950423015
1112	6	-0.01646346161904333
1112	7	0.4542063579098148
1112	8	-3.413431833479777
1112	9	-0.5590570768258658
1112	10	-2.9994735879639762
1112	11	-0.6120490826795439
1112	12	0.6590720927618179
1112	13	1.055571325945202
1112	14	1.7851114795211132
1112	15	1.111438308463569
1112	16	0.8479118169495621
1112	17	0.6139949657757029
1112	18	0.7350286408905298
1112	19	0.8435817391212481
1112	20	0.9375277619709763
1112	21	0.9134701495636435
1112	22	0.8157254107100016
1112	23	0.6262486801710381
1112	24	0.7965398538215922
1112	25	0.8414150881234406
1113	1	-1.2812354785119808
1113	2	-0.8192155614980621
1113	3	-3.3161811727257486
1113	4	-1.508265030251927
1113	5	-1.742020252306272
1113	6	-1.587806196910229
1113	7	-1.2783643338778035
1113	8	-8.867263131227183
1113	9	-9.811256151879036
1113	10	-3.7755333403666214
1113	11	-9.278905676034178
1113	12	-8.025294019414698
1113	13	-3.2607973945588817
1113	14	-1.5296142762816416
1113	15	-7.624620841857375
1113	16	-3.3915959051735705
1113	17	-2.567418394076949
1113	18	-2.001027508473747
1113	19	-1.6730194822728386
1113	20	-7.133945125047166
1113	21	-3.279875746514694
1113	22	-2.47873672782654
1113	23	-2.361462922127629
1113	24	-7.994513103422715
1113	25	-5.068677597013963
1114	1	-3.9827342599581144
1114	2	-4.0890699684041465
1114	3	-0.6950868164084554
1114	4	-0.37568161644021103
1114	5	-0.33118834286700505
1114	6	-0.564846976071717
1114	7	-0.3136168732100195
1114	8	0.04366283305479257
1114	9	-0.24788435171925727
1114	10	-3.2929371700326477
1114	11	-1.2042479483207487
1114	12	-4.549496225799899
1114	13	-1.1950820424200437
1114	14	-5.908684453552738
1114	15	-2.0576962046815055
1114	16	-1.15243817074618
1114	17	-1.0246304992144601
1114	18	-0.5755604991456202
1114	19	-0.3419608382763002
1114	20	-0.10461018606754635
1114	21	0.07630579561159578
1114	22	-0.0380444318985127
1114	23	-0.41251261100989467
1114	24	-0.25862095551075015
1114	25	-0.11603161385685112
1115	1	0.9564490409352793
1115	2	1.0766110879585256
1115	3	1.8387683810247601
1115	4	0.7036193191586371
1115	5	0.46772850293268675
1115	6	-0.2131708860236401
1115	7	-2.121088049258116
1115	8	0.01903587818462782
1115	9	0.2488787282535192
1115	10	1.1072456418081558
1115	11	0.13794461722894624
1115	12	0.9662971663003006
1115	13	1.2300226421206126
1115	14	1.7750943340222989
1115	15	0.9269026834145144
1115	16	0.6296894615988187
1115	17	0.19493742472183584
1115	18	0.5333128073020177
1115	19	0.7722194914850355
1115	20	0.7669974377523623
1115	21	0.8295403756064132
1115	22	0.4610143985583939
1115	23	0.4233643347128684
1115	24	0.3595326244750474
1115	25	0.6232393545778976
1116	1	0.6500686048207953
1116	2	0.8176398018739341
1116	3	1.6888533035268587
1116	4	0.9910685864527942
1116	5	0.13847178634650703
1116	6	-0.8167603341360987
1116	7	-4.4526412133414155
1116	8	-0.6476217162037372
1116	9	-0.7669765156968147
1116	10	0.04476362104730569
1116	11	-1.801963867838328
1116	12	-0.106303725352479
1116	13	-6.374715261614238
1116	14	-1.283460352080823
1116	15	-0.8910511114480464
1116	16	-0.4120390784524375
1116	17	-0.05622011520194192
1116	18	0.11961105290339447
1116	19	0.33957630398453986
1116	20	0.5542722019547099
1116	21	0.6872047589513599
1116	22	0.42262768598002154
1116	23	0.25697887775705297
1116	24	0.476296700478958
1116	25	0.39225404435184075
1201	1	2.2169938106116334
1201	2	2.411236669149943
1201	3	3.0807404122989226
1201	4	2.5367123401704603
1201	5	2.0840436967842635
1201	6	1.992844643844606
1201	7	2.3093235263337917
1201	8	2.3967188182656436
1201	9	2.736914779619674
1201	10	3.012705810857252
1201	11	2.530135999675526
1201	12	2.7982993539129737
1201	13	2.9532344293481003
1201	14	3.5715716226410485
1201	15	3.0697110788170505
1201	16	2.5773398520108515
1201	17	2.3502989484793204
1201	18	2.465811301989845
1201	19	2.483113398544127
1201	20	2.621522108134524
1201	21	2.465796197820538
1201	22	1.5410713731199408
1201	23	1.1106523228297398
1201	24	1.6368966855748255
1201	25	1.6777146601781574
1202	1	1.9303926045675688
1202	2	2.1790580793401446
1202	3	2.9694364044733845
1202	4	2.4794893656718813
1202	5	1.9285634376543435
1202	6	1.8351481224922743
1202	7	2.0579363398576156
1202	8	2.3364762937848162
1202	9	2.476040364375521
1202	10	-1.267418022782092
1202	11	1.1073761000609679
1202	12	2.228386223538532
1202	13	2.8017801299764926
1202	14	3.316763957607929
1202	15	2.794262603411198
1202	16	2.412453070274104
1202	17	2.0209252282898476
1202	18	2.230884805520766
1202	19	2.4783760924121907
1202	20	2.4579767703385436
1202	21	2.649243485030432
1202	22	2.280587378863494
1202	23	2.022827567477691
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2211	18	0.47611119119246936
2211	19	0.8962725125531392
2211	20	1.0307922650116375
2211	21	1.109101474294473
2211	22	0.9381861092184458
2211	23	0.8049532291317867
2211	24	0.9575751249181934
2211	25	0.99127755122446
2212	1	0.83877232577494
2212	2	0.876408164473915
2212	3	1.6781487711798206
2212	4	1.1692404887415435
2212	5	0.6923024108797904
2212	6	0.560953848899679
2212	7	0.7675857323344566
2212	8	1.1049235581453054
2212	9	0.9574661098158437
2212	10	1.8265596091828478
2212	11	0.9819985495016574
2212	12	1.1906171047930592
2212	13	1.3531032979037718
2212	14	-4.4902987041897084
2212	15	-1.1110522007710024
2212	16	-0.5003000456185127
2212	17	-0.01649561210373207
2212	18	0.3542397643005124
2212	19	0.8279182068281301
2212	20	1.0644738132364324
2212	21	1.0135390395884407
2212	22	0.9461067697067094
2212	23	0.8281399687699853
2212	24	0.9009961313331658
2212	25	0.9100635075141454
2213	1	0.9824343315526356
2213	2	1.046111031735414
2213	3	1.734041131348885
2213	4	1.185567144282821
2213	5	0.6594203958707539
2213	6	0.3719079511303889
2213	7	0.5685997401700043
2213	8	-5.0063607970344
2213	9	-1.3749357175973174
2213	10	0.5488006474442848
2213	11	0.2977192169566578
2213	12	0.9162119269660245
2213	13	-7.922956258752947
2213	14	-2.0665977467579673
2213	15	-0.526051946951771
2213	16	0.03814633752140395
2213	17	0.23162172106237833
2213	18	0.5305931113870095
2213	19	0.8171565699628296
2213	20	1.01441071036243
2213	21	1.1148274174830455
2213	22	0.6172350239252253
2213	23	0.6516775860600639
2213	24	0.768939658046893
2213	25	0.9092013113080384
2214	1	-0.7084322288810974
2214	2	-0.34933722975992865
2214	3	-6.434940249931756
2214	4	-2.2135757101546147
2214	5	-1.3318853899463363
2214	6	-0.8824893753708487
2214	7	-0.47155583516519783
2214	8	-6.266648843394524
2214	9	-1.929766858636416
2214	10	-0.029725262389384
2214	11	-0.4408730298428267
2214	12	-0.0026778081189817726
2214	13	-6.181540903314506
2214	14	-1.4175215133557337
2214	15	-0.7029277518150394
2214	16	-0.5122749644362408
2214	17	-0.36565547384709873
2214	18	-0.25957260928350223
2214	19	-0.05529390727374306
2214	20	0.20526780181653947
2214	21	0.2321879799945421
2214	22	-0.007990540696500403
2214	23	-0.04467692442074167
2214	24	0.081034859168021
2214	25	0.2119120772673284
2215	1	0.2849885772438121
2215	2	0.7199399993732456
2215	3	-2.601145687837832
2215	4	-0.5624620162800369
2215	5	0.02450731877143042
2215	6	-0.525691805001653
2215	7	0.2304437128168182
2215	8	0.6216084873027192
2215	9	0.8883584223811845
2215	10	1.5360518893305866
2215	11	0.7191616819120661
2215	12	0.4765860802530604
2215	13	1.050799295610961
2215	14	1.2487661173974658
2215	15	0.8479262237582361
2215	16	0.5207875030664333
2215	17	0.4333046202026871
2215	18	0.5296577357864791
2215	19	0.4836866372611226
2215	20	0.8471467388368481
2215	21	0.975295580588174
2215	22	0.7705452176302394
2215	23	0.5725338416814587
2215	24	0.5292629141045921
2215	25	0.6165992182604079
2216	1	0.18855745062546703
2216	2	-0.01886029961753266
2216	3	1.2235394721839867
2216	4	-0.2611402058143959
2216	5	-0.3672076330793317
2216	6	-0.7327609960284676
2216	7	-1.2947295189262604
2216	8	-6.401217688741063
2216	9	-2.1895023266663713
2216	10	-0.7759688679629946
2216	11	-1.4104817466491149
2216	12	-0.20411299446349673
2216	13	0.5225998349042449
2216	14	1.2307535325377401
2216	15	0.7790781822319168
2216	16	0.001657111778708753
2216	17	-0.1518887642245268
2216	18	0.11586262826779503
2216	19	0.2664119759195813
2216	20	0.3248972702099202
2216	21	0.13317433651828736
2216	22	-0.11873902407693038
2216	23	-0.3261139335018406
2216	24	-0.2617426133488685
2216	25	-0.256595292495696
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	556093.0
3	12.0
4	4118.0
5	24773.0
6	31659.0
7	28847.0
8	25747.0
9	22377.0
10	19996.0
11	18384.0
12	17264.0
13	17096.0
14	17582.0
15	17876.0
16	19142.0
17	20631.0
18	23798.0
19	29719.0
20	40113.0
21	51023.0
22	63305.0
23	75283.0
24	87076.0
25	100212.0
26	111855.0
27	124132.0
28	136679.0
29	158471.0
30	180650.0
31	203543.0
32	251770.0
33	313773.0
34	386785.0
35	476207.0
36	587884.0
37	675299.0
38	875268.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.802359240340685	17.448929367228825	13.791919795804064	23.95679159662643
2	14.987205454849327	19.2523655836694	40.830073719095004	24.93035524238627
3	17.328309435000243	24.157946208744328	29.420516087595004	29.093228268660432
4	11.506957754603066	15.621399761478107	37.24385095932743	35.6277915245914
5	14.250118358191296	36.88207539897494	33.67905069541609	15.18875554741768
6	33.4937263556771	35.1461195783823	17.426805174587983	13.933348891352626
7	30.079909634397794	30.584008827406233	20.54986374691398	18.786217791281985
8	26.328626246351345	34.319472124789144	20.111452018687068	19.240449610172448
9	26.111937837128913	16.211032420645125	19.55502229563203	38.12200744659393
10	13.813766602539024	29.033753765455074	33.27827681474611	23.874202817259793
11	35.30306671394419	23.126050932299876	22.16229234131788	19.40859001243805
12	23.38684893910357	24.378898037561054	29.965391945009394	22.26886107832598
13	28.182340382656434	21.290553112987453	26.25583163615438	24.271274868201733
14	21.637561457507708	20.39802338735396	28.097460119925028	29.866955035213305
15	24.28922885266812	28.429045453656247	22.94409271433065	24.337632979344974
16	24.34957270668957	27.854049582713397	24.452115483630376	23.344262226966656
17	23.147146801955447	27.48904480459823	25.595331969296886	23.768476424149434
18	22.884658830739294	27.10465109233543	26.950004198127374	23.060685878797898
19	24.27317129244519	26.279763331966265	26.65622872254695	22.790836653041595
20	24.220487601800535	25.93316507515243	26.918550898099724	22.92779642494731
21	24.636245504464288	25.507843621316535	26.605382766712037	23.250528107507144
22	24.33206957629139	25.45473526283798	26.309472222303974	23.903722938566656
23	23.918032591066098	25.720666750800792	26.284313435267187	24.076987222865924
24	23.950656824115175	25.880982223853415	26.424723178239283	23.743637773792127
25	23.893356146728863	25.600812086714214	26.6270513576642	23.878780408892723
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	18009.0
1	18009.0
2	10467.0
3	2925.0
4	2925.0
5	2925.0
6	4771.0
7	6617.0
8	6617.0
9	6617.0
10	9110.5
11	11604.0
12	11604.0
13	11604.0
14	17417.5
15	23231.0
16	23231.0
17	23231.0
18	39466.5
19	55702.0
20	55702.0
21	55702.0
22	84244.5
23	112787.0
24	112787.0
25	112787.0
26	158498.0
27	204209.0
28	204209.0
29	204209.0
30	262656.5
31	321104.0
32	321104.0
33	321104.0
34	380933.5
35	440763.0
36	440763.0
37	440763.0
38	500027.5
39	559292.0
40	559292.0
41	559292.0
42	612888.5
43	666485.0
44	666485.0
45	666485.0
46	706606.5
47	746728.0
48	746728.0
49	746728.0
50	750778.5
51	754829.0
52	754829.0
53	754829.0
54	708596.5
55	662364.0
56	662364.0
57	662364.0
58	588306.5
59	514249.0
60	514249.0
61	514249.0
62	426715.0
63	339181.0
64	339181.0
65	339181.0
66	267233.5
67	195286.0
68	195286.0
69	195286.0
70	145841.0
71	96396.0
72	96396.0
73	96396.0
74	67123.0
75	37850.0
76	37850.0
77	37850.0
78	27054.0
79	16258.0
80	16258.0
81	16258.0
82	10932.5
83	5607.0
84	5607.0
85	5607.0
86	3861.5
87	2116.0
88	2116.0
89	2116.0
90	1387.5
91	659.0
92	659.0
93	659.0
94	392.5
95	126.0
96	126.0
97	126.0
98	95.5
99	65.0
100	65.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.35171635163489423
2	0.3537355279421211
3	0.3664891287202461
4	0.3690950742107696
5	0.37468663936924385
6	0.3143357030754644
7	0.32432803710866376
8	0.32242966622152747
9	0.3218083812039192
10	0.435227412751737
11	0.32130790160640144
12	0.3213251595235572
13	0.31392151306372557
14	0.31927146738201884
15	0.31193685259081033
16	0.3160960106253544
17	0.30993493420073925
18	0.3116262100820062
19	0.312040400093745
20	0.5089704927584053
21	0.31333474388042887
22	0.3154574676905904
23	0.309796870863493
24	0.3134382913833636
25	0.3057930340833509
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	5794442.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	68.42664146212539
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.10141001636545	60.96910236959666
2	5.952702590563601	8.146468917903212
3	2.0257876782922977	4.158535414226954
4	0.907187919786266	2.4830329010394476
5	0.4852681098452564	1.660263348269232
6	0.2974519256809522	1.2212181762473568
7	0.2004236900492329	0.9600023985660505
8	0.15576532558855652	0.8526798469023507
9	0.11370159388613603	0.7002196378666936
>10	0.6532569668672712	8.464410946857983
>50	0.06238689604468583	2.963814417354467
>100	0.042234321431897426	5.433282881948015
>500	0.0017984968986331836	0.8350796129740856
>1k	5.74511203771928E-4	0.7225009337566888
>5k	2.497874798032114E-5	0.14345414907841122
>10k+	2.497874798032114E-5	0.2859340474124558
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
NNNNNNNNNNNNNNNNNNNNNNNNN	16729	0.28870769609912394	No Hit
GTATCAACGCAGAGTACTTTTTTTT	8393	0.14484569868850183	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.72579171557848E-5
2	0.0	0.0	0.0	0.0	1.72579171557848E-5
3	0.0	0.0	0.0	0.0	1.72579171557848E-5
4	0.0	0.0	0.0	0.0	1.72579171557848E-5
5	0.0	0.0	0.0	0.0	1.72579171557848E-5
6	0.0	0.0	0.0	0.0	1.72579171557848E-5
7	0.0	0.0	0.0	0.0	1.72579171557848E-5
8	0.0	0.0	0.0	0.0	1.72579171557848E-5
9	0.0	0.0	0.0	1.72579171557848E-5	1.72579171557848E-5
10	0.0	0.0	0.0	1.72579171557848E-5	1.72579171557848E-5
11	0.0	0.0	0.0	6.90316686231392E-5	1.72579171557848E-5
12	0.0	0.0	0.0	6.90316686231392E-5	5.177375146735441E-5
13	0.0	0.0	0.0	8.628958577892401E-5	8.628958577892401E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGCATAT	225	0.0	12.667105	8
GGTATCA	3030	0.0	12.62798	1
GTATCAA	8005	0.0	11.338824	1
GGACCGA	530	0.0	11.113882	6
AGGACCG	630	0.0	10.862886	5
GTATTAG	695	0.0	9.972626	1
TCGTTCG	410	0.0	9.970155	14
ACCGAGT	495	0.0	9.788218	8
GTCTAGG	560	0.0	9.664031	1
GTCCTAC	515	0.0	9.586666	1
ACGCATA	675	0.0	9.5707855	7
TAACGCA	150	2.6316593E-7	9.50519	4
AACGCAT	720	0.0	9.500576	6
ACCGTTT	110	6.840742E-5	9.500329	8
TGGTATC	1180	0.0	9.494651	2
CGCAGAT	1055	0.0	9.455305	8
CGCGCCA	265	0.0	9.320996	10
TGCCGTA	175	2.6471753E-8	9.234493	15
CGAGTGG	505	0.0	9.218061	10
GTATTAC	485	0.0	9.200843	1
>>END_MODULE