FastQCFastQC Report
Thu 26 May 2016
SRR1364309_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364309_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5794442
Sequences flagged as poor quality0
Sequence length25
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT159560.2753673261377023No Hit
GGTATCAACGCAGAGTACTTTTTTT109170.1884046815897027No Hit
TATCAACGCAGAGTACTTTTTTTTT108900.18793871782649652No Hit
GTGGTATCAACGCAGAGTACATGGG60700.10475555713561374No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA49000.012.7590851
GGACCGA6450.012.520536
CGCGCCA2950.012.23798810
AGGACCG8000.011.7603765
ACCGAGT7200.011.0842548
TGGTATC17300.010.8185392
TAGGACG1252.2398126E-710.6414364
CGAGTGG7600.010.37559410
GTGGTAT20100.010.1632391
TAACGCA1701.5588739E-910.0601824
ACGCGGG951.649365E-49.99772413
CGCCCGA951.6496523E-49.99755116
TTTACGG3950.09.85783418
GTATCAA101450.09.7496361
GTTCGCG4500.09.70873416
TCGTTCG4600.09.70439614
TTCGCGC4750.09.59756717
TAGGACA7850.09.5618014
ATCCTAG10750.09.5456391
TAGACCG1106.817132E-59.5033335
Produced by FastQC (version 0.11.5)