FastQCFastQC Report
Thu 26 May 2016
SRR1364308_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364308_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2334065
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT197410.8457776454383232No Hit
TATCAACGCAGAGTACTTTTTTTTT120500.516266684946649No Hit
GGTATCAACGCAGAGTACTTTTTTT87370.3743254793675412No Hit
NNNNNNNNNNNNNNNNNNNNNNNNN69560.29802083489534353No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA61070.2616465265534593No Hit
GTACTTTTTTTTTTTTTTTTTTTTT61020.2614323080119877No Hit
ACGCAGAGTACTTTTTTTTTTTTTT44400.190226064826815No Hit
GAGTACTTTTTTTTTTTTTTTTTTT38930.16679055638981777No Hit
GTGGTATCAACGCAGAGTACATGGG34540.1479821684486079No Hit
GAAAAAAAAAAAAAAAAAAAAAAAA24750.10603817802846108No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCACGC1350.016.1743223
CGCATAT1800.015.3054878
TATCACG1500.013.9239812
TATAACG2800.013.9013782
TAACGCA3150.013.8798244
ATAACGC2950.013.8381613
GGTATCA43750.012.9107711
TCACGCA1700.012.8592474
CCTATAC2050.012.5037973
TGACCGA2000.012.36005319
GAACCGC701.09095636E-412.2147566
GTTATCA5850.011.8462341
TTGACCG2200.011.6685818
CTCAACG1552.5465852E-1111.6373443
TCAACTC11450.011.5381615
GTGGTAT13300.011.2062771
CGCAGAT10950.011.1049738
GTATCAA109650.011.0905691
GGGCGTA600.0058773411.0832839
CGCGCCA951.3616045E-510.99947610
Produced by FastQC (version 0.11.5)