##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1364308_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2334065
Sequences flagged as poor quality	0
Sequence length	25
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	26.378456898158362	30.0	26.0	31.0	7.0	34.0
2	26.225365189058575	31.0	26.0	31.0	7.0	34.0
3	25.67980326169151	30.0	25.0	31.0	7.0	34.0
4	29.385190215353898	35.0	28.0	37.0	7.0	37.0
5	30.052600077547112	35.0	30.0	37.0	7.0	37.0
6	30.161007941081333	35.0	30.0	37.0	2.0	37.0
7	29.73179153108418	35.0	30.0	37.0	2.0	37.0
8	29.20729842570794	35.0	28.0	37.0	2.0	37.0
9	30.195790177223	37.0	28.0	39.0	2.0	39.0
10	29.563027593490325	35.0	27.0	39.0	2.0	39.0
11	30.17768956734281	35.0	28.0	39.0	2.0	39.0
12	29.710866663953233	35.0	27.0	39.0	2.0	39.0
13	29.276724941250563	35.0	27.0	39.0	2.0	39.0
14	29.584860747237116	36.0	25.0	40.0	2.0	41.0
15	30.313667357164434	36.0	27.0	40.0	2.0	41.0
16	30.666386326002062	37.0	28.0	40.0	2.0	41.0
17	30.865878628058773	37.0	29.0	40.0	2.0	41.0
18	30.727833629311952	37.0	29.0	40.0	2.0	41.0
19	30.744426997534344	37.0	28.0	40.0	2.0	41.0
20	30.639000199223243	37.0	28.0	40.0	2.0	41.0
21	30.557836221356304	37.0	28.0	40.0	2.0	41.0
22	30.64526951905795	37.0	29.0	40.0	2.0	41.0
23	30.65654598308102	37.0	29.0	40.0	2.0	41.0
24	30.56513464706424	37.0	29.0	40.0	2.0	41.0
25	30.382332111573586	37.0	29.0	40.0	2.0	41.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1101	1	0.8469585284033556
1101	2	1.0079488493114326
1101	3	1.7488613819826995
1101	4	1.1810644726354695
1101	5	0.7674423376547423
1101	6	0.6087483470085466
1101	7	0.8986951654088244
1101	8	1.0560838087285482
1101	9	0.9950784783021618
1101	10	1.4147048449567592
1101	11	0.7580999381431681
1101	12	1.064035701132319
1101	13	0.9895325189639763
1101	14	1.571272452434492
1101	15	0.8906155102735305
1101	16	0.34935897085160406
1101	17	-0.22884414125682895
1101	18	-5.821348638740364
1101	19	-6.796901076781705
1101	20	-8.214377266485872
1101	21	-9.913605439663595
1101	22	-9.853586498632303
1101	23	-11.328464377876713
1101	24	-11.06417134473407
1101	25	-11.553916199933166
1102	1	1.918175390802599
1102	2	2.0445730918385188
1102	3	2.816328620643745
1102	4	2.234241177103357
1102	5	1.5991302851846214
1102	6	1.6051040536446521
1102	7	2.025181657514473
1102	8	2.2049440622277245
1102	9	2.459901420083206
1102	10	-0.9820869049690586
1102	11	1.0534995079243892
1102	12	1.9566433586352439
1102	13	2.5019975814597437
1102	14	3.10106092183128
1102	15	2.584111459028847
1102	16	2.26480023556212
1102	17	2.018936774010619
1102	18	2.1592607884351516
1102	19	1.9832132059100367
1102	20	2.2472964652653324
1102	21	2.351641437764716
1102	22	2.1864465344406696
1102	23	2.244169017538862
1102	24	2.3914967976279584
1102	25	2.3919865631623125
1103	1	-0.026199871225802696
1103	2	0.37144132839256727
1103	3	-1.9163692030158117
1103	4	0.16018209255275906
1103	5	-1.8018136396998763
1103	6	-7.159429442405724
1103	7	-7.330724514525592
1103	8	-2.36836577675712
1103	9	-6.971165912395033
1103	10	-3.989427851135453
1103	11	-7.707559647192273
1103	12	-1.5499358891305093
1103	13	-0.25455987384495415
1103	14	0.5264248559308768
1103	15	0.4294036647994943
1103	16	-0.006922697163513902
1103	17	-0.1073183679724572
1103	18	-0.5002475736434775
1103	19	-6.399159391211697
1103	20	-2.088535736935494
1103	21	-7.284451443779766
1103	22	-3.0349544925031147
1103	23	-1.8881402776721927
1103	24	-6.405341427594017
1103	25	-2.7955427528864867
1104	1	1.531778847760151
1104	2	1.4982157845305863
1104	3	2.4096074227556485
1104	4	1.8545540866718255
1104	5	1.4147634562141995
1104	6	1.3111264223327481
1104	7	1.4515437990246483
1104	8	1.886287887614209
1104	9	2.0444958174972356
1104	10	2.2831510389624796
1104	11	1.755917082409269
1104	12	2.1525556175686056
1104	13	2.234747704089905
1104	14	2.810105258625473
1104	15	1.8835419166331349
1104	16	1.1575815349507153
1104	17	0.9946656172753663
1104	18	0.9489192937613495
1104	19	1.5091358018707304
1104	20	-9.219070918721208
1104	21	-3.326116379945031
1104	22	-0.8566491656044768
1104	23	0.21716627026823332
1104	24	0.9123454042593941
1104	25	1.2773044341488706
1105	1	1.4830192842953167
1105	2	1.4869633802738704
1105	3	2.344377305136419
1105	4	1.6157523253708383
1105	5	1.3780075235014024
1105	6	1.449024237423906
1105	7	1.6025694442284788
1105	8	1.852009086172771
1105	9	1.7427311971721444
1105	10	2.3195017107059215
1105	11	1.8827097511387514
1105	12	2.2037994036173707
1105	13	2.292962703030735
1105	14	2.9943197554410226
1105	15	2.2581073097000655
1105	16	1.7481907058585904
1105	17	1.2435768744775757
1105	18	1.7505663793525876
1105	19	1.7803548196243604
1105	20	1.718956441071473
1105	21	1.6964116639329951
1105	22	1.6420448258509452
1105	23	1.5563055780474961
1105	24	1.4502935827576877
1105	25	1.556830094742356
1106	1	1.0383529975800663
1106	2	1.3347975807346444
1106	3	1.9746890151961694
1106	4	1.5130681613603123
1106	5	0.7425268528412694
1106	6	0.671235854453684
1106	7	1.1087415132719514
1106	8	1.1095545021446185
1106	9	1.4723653272103618
1106	10	-1.7864493624426778
1106	11	0.0836324640652677
1106	12	0.7480140315020698
1106	13	1.5776527718900475
1106	14	0.7570514164374806
1106	15	1.3597224397202332
1106	16	0.9623913746587363
1106	17	-6.047731752224443
1106	18	-1.8712407821503199
1106	19	-0.21050493211012622
1106	20	0.5389321266283318
1106	21	0.7941728262294561
1106	22	0.9095809449439933
1106	23	0.4083128197623651
1106	24	0.8161041962507376
1106	25	0.8840276104352398
1107	1	0.7411451748439717
1107	2	1.1118754302139422
1107	3	1.5801872607885912
1107	4	1.0839948939401545
1107	5	0.5148035018739598
1107	6	0.645061502716036
1107	7	0.6322574713717124
1107	8	0.7462596866958577
1107	9	0.8746089976176314
1107	10	-1.8509097361803981
1107	11	-0.004439878258544638
1107	12	0.4320198564387425
1107	13	1.2275663849900234
1107	14	1.5154069957060088
1107	15	1.2114534006079047
1107	16	-5.453117444107271
1107	17	-1.9804564982737034
1107	18	-0.10817415312657275
1107	19	0.7084979020773581
1107	20	1.0698835635026391
1107	21	1.2814883234488335
1107	22	0.8836763691503258
1107	23	0.5220449787527244
1107	24	0.8060264007116018
1107	25	1.105960882996989
1108	1	-0.7792039274964928
1108	2	-3.2545828057822526
1108	3	-0.3705316488156427
1108	4	-1.0730132060726874
1108	5	-1.2311955352540878
1108	6	-0.7791678404966902
1108	7	-0.2586398796111453
1108	8	-0.12055391650984504
1108	9	-0.7650230146834147
1108	10	-3.7115266851165742
1108	11	-1.8087142220467882
1108	12	-6.309155745783514
1108	13	-2.1433663469239477
1108	14	-6.533035325223782
1108	15	-3.121651516588283
1108	16	-8.09948789631072
1108	17	-3.9556299505961903
1108	18	-2.3888188084536566
1108	19	-1.2899473758343198
1108	20	-1.2251985747991085
1108	21	-0.6754640703527883
1108	22	-0.8368605582596267
1108	23	-0.7871912713949349
1108	24	-0.6306297534180914
1108	25	-0.8436373229090783
1109	1	-8.494311830513332
1109	2	-6.156029409520919
1109	3	-5.179176327697746
1109	4	-5.1206199430706505
1109	5	-2.55410473384746
1109	6	-1.600542928410814
1109	7	-1.3020344381897146
1109	8	-0.6428736647978326
1109	9	-5.578201026898849
1109	10	-1.567294517802118
1109	11	-1.173469061950545
1109	12	-6.009021894219568
1109	13	-7.960194479442919
1109	14	-7.8690967725678505
1109	15	-3.7120029509765864
1109	16	-2.2458947073416304
1109	17	-1.9237438132650766
1109	18	-1.2136053344577284
1109	19	-0.9825181698376504
1109	20	-0.7857184341365127
1109	21	-0.7097562783853633
1109	22	-0.9548567825794478
1109	23	-1.1466481657129073
1109	24	-0.9453088576194624
1109	25	-0.8401269768050632
1110	1	-2.552797646116172
1110	2	-2.462398203847158
1110	3	-1.7572373206055474
1110	4	-2.5075355459075297
1110	5	-5.334106152287177
1110	6	-4.036615978056204
1110	7	-8.660349331806735
1110	8	-9.474155934301379
1110	9	-11.129920175835021
1110	10	-8.81522572347588
1110	11	-11.712608778262602
1110	12	-6.5670566459926185
1110	13	-4.920919302137953
1110	14	-4.417908829305439
1110	15	-9.559229192111339
1110	16	-11.130791870462197
1110	17	-12.012964380640963
1110	18	-8.577220937294609
1110	19	-11.830869233667439
1110	20	-12.672111341937931
1110	21	-13.493851313846356
1110	22	-14.273603591090087
1110	23	-15.031962350110069
1110	24	-15.395529424995347
1110	25	-16.05417520375542
1111	1	-0.7599122544666983
1111	2	0.08528696567780969
1111	3	-2.833077334586985
1111	4	-3.2458786606117194
1111	5	-1.0712040449232418
1111	6	-0.4800138346111318
1111	7	-0.11995537088711927
1111	8	0.23495060653231903
1111	9	-4.361908923030274
1111	10	-4.156889430361737
1111	11	-1.3744751032859988
1111	12	-5.885255492523754
1111	13	-1.2381438170157857
1111	14	0.07974265509510658
1111	15	0.0936754803959623
1111	16	0.007122780734682976
1111	17	-0.018265911834234316
1111	18	0.3032290057648481
1111	19	0.35232019794189284
1111	20	0.3359536379355603
1111	21	0.588263435446116
1111	22	0.12872265568234198
1111	23	-0.010049119607977275
1111	24	0.11759776665186905
1111	25	0.27922352663955863
1112	1	-0.22976961299174192
1112	2	0.26569736643536856
1112	3	-2.290871162881949
1112	4	-0.4111986139678834
1112	5	-0.4389215150919519
1112	6	-0.22344904510391927
1112	7	0.19573266916089338
1112	8	-3.696757394746715
1112	9	-0.8551139830067278
1112	10	-3.2136478405590374
1112	11	-0.897723177938559
1112	12	0.19883793303232622
1112	13	0.7059501353993163
1112	14	1.3580035270972282
1112	15	0.6936485209077787
1112	16	0.522618601313404
1112	17	0.2781858888325779
1112	18	0.3539190089231994
1112	19	0.5146367746361733
1112	20	0.4675656836142359
1112	21	0.4208541217556565
1112	22	0.24906664417887825
1112	23	0.18272775531653096
1112	24	0.38164675669395365
1112	25	0.3995799860012106
1113	1	-2.0308708484547893
1113	2	-1.2875232716550045
1113	3	-3.7679755722680923
1113	4	-2.0854947306060616
1113	5	-2.503004450561342
1113	6	-2.403008650790415
1113	7	-2.2120038432888656
1113	8	-9.391125978301137
1113	9	-10.617613302630673
1113	10	-4.516812175970202
1113	11	-9.690663396228164
1113	12	-8.044630581660552
1113	13	-3.8640214860529305
1113	14	-2.6422601342297973
1113	15	-8.439985576094625
1113	16	-4.484036350401119
1113	17	-3.3985336149647942
1113	18	-2.7727320577816172
1113	19	-2.6984010782418046
1113	20	-7.663880473796571
1113	21	-4.166033521067138
1113	22	-3.427075101748475
1113	23	-3.4047626133049746
1113	24	-8.011811596051945
1113	25	-5.343034625530276
1114	1	-4.387653724574328
1114	2	-4.29992026024323
1114	3	-1.1319823202071078
1114	4	-0.9193456787581837
1114	5	-1.1052640703910654
1114	6	-1.0137153200641045
1114	7	-0.8448603343908907
1114	8	-0.5536426321600594
1114	9	-0.8598070194082119
1114	10	-3.6999682470691084
1114	11	-1.6216235830848404
1114	12	-5.290334681806513
1114	13	-1.7566122429528193
1114	14	-6.3565125029049305
1114	15	-2.5103751286116847
1114	16	-1.6137234927883917
1114	17	-1.3979644660205608
1114	18	-1.0070152863036341
1114	19	-0.720035558815713
1114	20	-0.5156796968438435
1114	21	-0.42574429336663755
1114	22	-0.562993326185655
1114	23	-0.8524433503035951
1114	24	-0.7682635927719481
1114	25	-0.660409052781521
1115	1	0.30061670990517797
1115	2	0.40338050625576116
1115	3	1.180778591117921
1115	4	-0.04402284313142957
1115	5	-0.2044739619450553
1115	6	-0.5555438487893838
1115	7	-2.3611773399870586
1115	8	-0.3798181468678159
1115	9	-0.27075888050655905
1115	10	0.3951775148452725
1115	11	-0.32238061877957946
1115	12	0.34386298899654477
1115	13	0.6554357714904846
1115	14	1.2139856273626854
1115	15	0.11097726956695553
1115	16	-0.17152603494430352
1115	17	-0.49688188741633255
1115	18	-0.09983417877482381
1115	19	0.17988870695318226
1115	20	0.18621377043064768
1115	21	0.27381854144618956
1115	22	-0.19704047624967558
1115	23	-0.18065542454767325
1115	24	-0.35686943763161594
1115	25	-0.07141373550958718
1116	1	0.271993717829087
1116	2	0.46600088564051134
1116	3	1.3187347949543806
1116	4	0.6759765426906235
1116	5	-0.27561443695487853
1116	6	-1.0697223969471246
1116	7	-4.5692290369952175
1116	8	-0.9956847441711574
1116	9	-1.1167848752499268
1116	10	-0.3775088690528783
1116	11	-1.9907814137206472
1116	12	-0.45377072136403385
1116	13	-6.331678734614737
1116	14	-1.4728585660414062
1116	15	-1.1109288749790096
1116	16	-0.7843404790026689
1116	17	-0.4816872738380873
1116	18	-0.1654272335315632
1116	19	0.03418398544669188
1116	20	0.25371104738343675
1116	21	0.336025838813633
1116	22	0.020077312460799135
1116	23	-0.09323125235706797
1116	24	-0.05197628599304238
1116	25	-0.15529069185743083
1201	1	2.670916942197085
1201	2	2.7951882397189785
1201	3	3.480846957433883
1201	4	3.0790911433216372
1201	5	2.480100531261918
1201	6	2.3200282247223
1201	7	2.8579393104198516
1201	8	2.943102011879958
1201	9	3.284631453635079
1201	10	3.5748101911337358
1201	11	3.0022111753501584
1201	12	3.446663385721102
1201	13	3.621065290672025
1201	14	4.077236189983129
1201	15	3.535991561444618
1201	16	3.0115817479790934
1201	17	2.9474541103537604
1201	18	3.079219433803299
1201	19	3.13280997254277
1201	20	3.1772111913733134
1201	21	2.975682802074406
1201	22	2.2639739181991914
1201	23	1.7815032617991413
1201	24	2.2411126477568857
1201	25	2.2978731715078737
1202	1	2.2270433607434974
1202	2	2.328265672446296
1202	3	3.0209832351437846
1202	4	2.5370915323391863
1202	5	2.092957561234506
1202	6	1.895006798365145
1202	7	2.136046436127227
1202	8	2.4288727832895987
1202	9	2.669114820095686
1202	10	-0.7623786708075855
1202	11	1.3381396812188555
1202	12	2.287397777911579
1202	13	2.792791312906811
1202	14	3.4647283640441735
1202	15	2.7762855736135776
1202	16	2.4193839811829605
1202	17	2.0506343288114266
1202	18	2.22102635953134
1202	19	2.3383015022935005
1202	20	2.3611493254263287
1202	21	2.640728967516278
1202	22	2.288966168240222
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2211	15	-1.825673391228161
2211	16	-0.783842296440163
2211	17	-0.4280670837053293
2211	18	0.015617726338916782
2211	19	0.20252375888352248
2211	20	0.3773511675406347
2211	21	0.5359191933127505
2211	22	0.40420485264067807
2211	23	0.2276818133227323
2211	24	0.5030995281247748
2211	25	0.4628549938448323
2212	1	0.8757118686108285
2212	2	0.9085002143842864
2212	3	1.5867888448937038
2212	4	1.0068928853682984
2212	5	0.6297102069047291
2212	6	0.48699504170768293
2212	7	0.6361199152363533
2212	8	0.9575896015597145
2212	9	0.8484025794896084
2212	10	1.7143330968295132
2212	11	0.8376637561844369
2212	12	1.1506945406078408
2212	13	1.2003170094803153
2212	14	-4.337525417596186
2212	15	-0.9669057845675191
2212	16	-0.3117411782329249
2212	17	0.0677566504134397
2212	18	0.39031800402758776
2212	19	0.8853952768393221
2212	20	1.220089705997438
2212	21	1.1802476371221076
2212	22	0.9084503786776352
2212	23	0.843018603993432
2212	24	0.7999906787020237
2212	25	0.9252924976926167
2213	1	0.5614639349027932
2213	2	0.6934177659857674
2213	3	1.3297868460840583
2213	4	0.7766036878047267
2213	5	0.24815624071053932
2213	6	0.06768510091217195
2213	7	0.18450217241830558
2213	8	-5.396856933448113
2213	9	-1.9143811045185153
2213	10	0.0026808163936529184
2213	11	0.013102391262048485
2213	12	0.46020898115774855
2213	13	-8.35352988263395
2213	14	-2.3789630942582356
2213	15	-1.0069738867247544
2213	16	-0.35597318944598655
2213	17	-0.24040547777564214
2213	18	0.12488401439953734
2213	19	0.43088415875226005
2213	20	0.549047585699622
2213	21	0.7423085771558178
2213	22	0.23799910357837817
2213	23	0.11441643109606403
2213	24	0.15985612728782783
2213	25	0.3235352914139966
2214	1	-0.9945958704995448
2214	2	-0.7044189303757946
2214	3	-6.88088525565362
2214	4	-2.538166857927596
2214	5	-1.6915667124755203
2214	6	-1.2038751586762366
2214	7	-0.7075450896891979
2214	8	-5.908523027086698
2214	9	-1.8487995791222218
2214	10	-0.10602557605789187
2214	11	-0.560581505935378
2214	12	-0.1812778059741369
2214	13	-5.8876088882674615
2214	14	-1.5497827429229005
2214	15	-1.0027821305698303
2214	16	-0.8308785060317376
2214	17	-0.7264165468052362
2214	18	-0.7224722079496289
2214	19	-0.19147370241262252
2214	20	-0.037135324863847075
2214	21	-0.08667678855427141
2214	22	-0.2926433389393246
2214	23	-0.4391558527173878
2214	24	-0.2240022736828884
2214	25	-0.14180529462650426
2215	1	-0.10937965537952721
2215	2	0.20784564531807703
2215	3	-3.003102553897623
2215	4	-0.8910155910481876
2215	5	-0.55424849097653
2215	6	-0.9708271786521045
2215	7	-0.2880817137885572
2215	8	0.007654228507423255
2215	9	0.2804792573864887
2215	10	0.8498719646055299
2215	11	-0.04054201097221721
2215	12	-0.2986855600965548
2215	13	0.4174590785109267
2215	14	0.44153409520437137
2215	15	0.019690582409392476
2215	16	-0.13392980429696877
2215	17	-0.30368583309485686
2215	18	-0.06921309088485827
2215	19	-0.07183641148018793
2215	20	0.22338845364097182
2215	21	0.34808343381960327
2215	22	0.1607549047066783
2215	23	-0.029098424552952196
2215	24	-0.15790789245318848
2215	25	-0.07298418484226588
2216	1	-0.14776801105121606
2216	2	-0.40442476033113195
2216	3	0.9423006214835574
2216	4	-0.7192882386505275
2216	5	-0.6859488332790384
2216	6	-0.9665293201983758
2216	7	-1.5973832801342311
2216	8	-6.33127322363384
2216	9	-2.335367125142678
2216	10	-1.0272237279488898
2216	11	-1.656584222720884
2216	12	-0.5114833178218419
2216	13	0.17278504036704234
2216	14	0.6786643201614275
2216	15	0.15521136988537876
2216	16	-0.2989409447217959
2216	17	-0.5695827205357986
2216	18	-0.2970831999027084
2216	19	-0.08654463967730308
2216	20	-0.07492389778290232
2216	21	-0.13883041090424086
2216	22	-0.4018242552187985
2216	23	-0.6097649553964573
2216	24	-0.5552465177535062
2216	25	-0.4520643315178816
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	210283.0
3	3.0
4	1203.0
5	9897.0
6	17070.0
7	15257.0
8	13220.0
9	10463.0
10	9477.0
11	8602.0
12	8114.0
13	7837.0
14	7747.0
15	7315.0
16	7507.0
17	7585.0
18	9198.0
19	12254.0
20	17449.0
21	22322.0
22	27001.0
23	31340.0
24	35378.0
25	39723.0
26	43469.0
27	46545.0
28	50345.0
29	58512.0
30	66911.0
31	75197.0
32	93752.0
33	117669.0
34	149860.0
35	187734.0
36	238373.0
37	284828.0
38	384625.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	46.68181739997936	18.54664763149747	13.644991227768413	21.126543740754755
2	14.186120915639771	21.836878444485727	41.95860940821929	22.018391231655208
3	15.837611612662933	29.998576415724454	30.43790914607738	23.72590282553523
4	10.181106890009897	17.838785664733123	37.913784879817605	34.06632256543937
5	11.781930794210629	41.03081786705091	32.50801092496613	14.679240413772341
6	28.378553218706465	38.55501543770393	18.69967379441698	14.366757549172624
7	27.3691685889406	35.33348694329643	20.636753419039668	16.6605910487233
8	26.180876851275908	34.94255472529135	20.296642609877225	18.579925813555512
9	27.86261231925643	17.61196891363212	19.625429814730992	34.89998895238046
10	13.127682135621171	33.342399303182674	31.676871620702773	21.853046940493385
11	32.48194353814661	26.544310011460187	22.074707025792733	18.89903942460046
12	22.7081222518208	30.078110727146345	28.69592354941059	18.51784347162226
13	26.802404825468763	25.05964997040605	26.77592715890769	21.362018045217493
14	20.65472083771254	24.381484180051455	29.441417508656322	25.522377473579688
15	23.25538213167101	31.050473643060666	24.54407637776424	21.150067847504083
16	21.430207902147107	31.964627362685587	25.448250990465652	21.156913744701647
17	20.691445579875996	31.02956062022501	26.415353040413482	21.86364075948551
18	20.040085156242277	29.08000569083711	30.03885156844027	20.84105758448035
19	21.69574955802917	28.653163470965755	30.233254861034396	19.417832109970682
20	22.452585024374507	27.59877393144301	29.89788935149036	20.050751692692124
21	23.476276913409002	27.201332136107244	29.395109970431836	19.927280980051915
22	22.58497962552226	27.70688261893709	29.27704991317206	20.431087842368598
23	21.72315053443619	27.963898693524015	29.390336994812948	20.922613777226847
24	21.42095110045889	28.01480689314863	29.79061966469574	20.77362234169674
25	21.414391046373442	27.494708068020397	30.06371808454627	21.02718280105989
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	14348.0
1	14348.0
2	12330.0
3	10312.0
4	10312.0
5	10312.0
6	15519.0
7	20726.0
8	20726.0
9	20726.0
10	21335.5
11	21945.0
12	21945.0
13	21945.0
14	24285.5
15	26626.0
16	26626.0
17	26626.0
18	37494.5
19	48363.0
20	48363.0
21	48363.0
22	65687.5
23	83012.0
24	83012.0
25	83012.0
26	110464.0
27	137916.0
28	137916.0
29	137916.0
30	161147.0
31	184378.0
32	184378.0
33	184378.0
34	196684.0
35	208990.0
36	208990.0
37	208990.0
38	221037.5
39	233085.0
40	233085.0
41	233085.0
42	248748.0
43	264411.0
44	264411.0
45	264411.0
46	273846.0
47	283281.0
48	283281.0
49	283281.0
50	269958.5
51	256636.0
52	256636.0
53	256636.0
54	229103.5
55	201571.0
56	201571.0
57	201571.0
58	173829.5
59	146088.0
60	146088.0
61	146088.0
62	119285.0
63	92482.0
64	92482.0
65	92482.0
66	71460.0
67	50438.0
68	50438.0
69	50438.0
70	38063.5
71	25689.0
72	25689.0
73	25689.0
74	18729.0
75	11769.0
76	11769.0
77	11769.0
78	9320.5
79	6872.0
80	6872.0
81	6872.0
82	4915.0
83	2958.0
84	2958.0
85	2958.0
86	2202.5
87	1447.0
88	1447.0
89	1447.0
90	1038.0
91	629.0
92	629.0
93	629.0
94	348.5
95	68.0
96	68.0
97	68.0
98	46.5
99	25.0
100	25.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.36609948737503023
2	0.36884148470586725
3	0.3833655018176443
4	0.38447943823329683
5	0.39052040110279707
6	0.3263833697861885
7	0.33533770481970293
8	0.3326385511971603
9	0.33298130086351496
10	0.44373228680435206
11	0.33208158298933405
12	0.3321244266976284
13	0.3245839340378267
14	0.3311818651151532
15	0.32235606120652166
16	0.3270688691188977
17	0.32089937512451455
18	0.3220989989567557
19	0.3236413724553515
20	0.5214936173585568
21	0.3245839340378267
22	0.32668327574424877
23	0.32137065591575215
24	0.32479815257929834
25	0.3174718784609683
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	2334065.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	56.110959509754174
#Duplication Level	Percentage of deduplicated	Percentage of total
1	83.17345984206476	46.669426374842615
2	8.623037256944958	9.676937887510807
3	3.0817491922655074	5.187597124392826
4	1.531498611374061	3.4373542628821876
5	0.8842673337542452	2.480854428004137
6	0.5817064019816093	1.958406262089292
7	0.39064706923319154	1.5343707319043423
8	0.26971074882652274	1.21069831254004
9	0.21010977464896008	1.0610514952138237
>10	1.0942229055011954	11.17444201518534
>50	0.09288516981571704	3.6231684450263764
>100	0.06078853721231742	6.198453664156746
>500	0.003865875499593641	1.5033301644907202
>1k	0.0015779083671810517	1.6413608324397575
>5k	3.1558167343621033E-4	1.2351929753414277
>10k+	1.5779083671810517E-4	1.4073550239796189
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	19741	0.8457776454383232	No Hit
TATCAACGCAGAGTACTTTTTTTTT	12050	0.516266684946649	No Hit
GGTATCAACGCAGAGTACTTTTTTT	8737	0.3743254793675412	No Hit
NNNNNNNNNNNNNNNNNNNNNNNNN	6956	0.29802083489534353	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA	6107	0.2616465265534593	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	6102	0.2614323080119877	No Hit
ACGCAGAGTACTTTTTTTTTTTTTT	4440	0.190226064826815	No Hit
GAGTACTTTTTTTTTTTTTTTTTTT	3893	0.16679055638981777	No Hit
GTGGTATCAACGCAGAGTACATGGG	3454	0.1479821684486079	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAA	2475	0.10603817802846108	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATCACGC	135	0.0	16.174322	3
CGCATAT	180	0.0	15.305487	8
TATCACG	150	0.0	13.923981	2
TATAACG	280	0.0	13.901378	2
TAACGCA	315	0.0	13.879824	4
ATAACGC	295	0.0	13.838161	3
GGTATCA	4375	0.0	12.910771	1
TCACGCA	170	0.0	12.859247	4
CCTATAC	205	0.0	12.503797	3
TGACCGA	200	0.0	12.360053	19
GAACCGC	70	1.09095636E-4	12.214756	6
GTTATCA	585	0.0	11.846234	1
TTGACCG	220	0.0	11.66858	18
CTCAACG	155	2.5465852E-11	11.637344	3
TCAACTC	1145	0.0	11.538161	5
GTGGTAT	1330	0.0	11.206277	1
CGCAGAT	1095	0.0	11.104973	8
GTATCAA	10965	0.0	11.090569	1
GGGCGTA	60	0.00587734	11.083283	9
CGCGCCA	95	1.3616045E-5	10.999476	10
>>END_MODULE