FastQCFastQC Report
Thu 26 May 2016
SRR1364308_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364308_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2334065
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT441261.8905214721955044No Hit
TATCAACGCAGAGTACTTTTTTTTT317021.3582312403467771No Hit
GGTATCAACGCAGAGTACTTTTTTT307841.318900716132584No Hit
GTACTTTTTTTTTTTTTTTTTTTTT143450.614592995482131No Hit
ACGCAGAGTACTTTTTTTTTTTTTT143040.6128364034420636No Hit
GAGTACTTTTTTTTTTTTTTTTTTT104890.4493876562992033No Hit
GTGGTATCAACGCAGAGTACTTTTT58980.2526921915199448No Hit
GTGGTATCAACGCAGAGTACATGGG56380.24155282736341963No Hit
ATCAACGCAGAGTACTTTTTTTTTT50600.2167891639692982No Hit
GCAGAGTACTTTTTTTTTTTTTTTT42220.18088613641865156No Hit
GGTATCAACGCAGAGTACATGGGAT35250.15102407173750518No Hit
GGTATCAACGCAGAGTACATGGGAG27660.11850569714211044No Hit
GTATAACGCAGAGTACTTTTTTTTT27420.11747744814304657No Hit
GGTATCAACGCAGAGTACATGGGAA24520.10505277273769154No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCACG1800.014.7775112
TATAACG3550.014.4504432
TCGACCG551.9639696E-413.81556318
GGTATCA71950.013.7740731
TAACGCA4150.013.73675254
ATAACGC4050.013.6061523
GTCGGTT1400.013.56711212
GGTCGGT1450.013.10124611
GTGGTAT19550.012.6367511
TGGTATC19500.012.2767022
TGGTCGG1551.8189894E-1212.25731710
TCACGCA2100.012.2158984
ATCACGC2100.012.2153743
CGAAATC1501.4551915E-1112.03131113
CATTCCG2500.011.780049
CGACCGT657.9740374E-411.70137619
TAGACTA1302.6066118E-911.6953545
TCGACGG658.0373924E-411.69034314
TCCGGTG2600.011.68858912
TCGCTCG907.478915E-611.60915917
Produced by FastQC (version 0.11.5)