##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1364308_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2334065 Sequences flagged as poor quality 0 Sequence length 25 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.440764503130804 31.0 30.0 34.0 26.0 34.0 2 29.4116247833715 31.0 30.0 34.0 25.0 34.0 3 29.43565496247962 31.0 30.0 34.0 25.0 34.0 4 32.74076214672685 35.0 33.0 37.0 26.0 37.0 5 32.35814469605602 35.0 32.0 37.0 24.0 37.0 6 32.21381666748784 35.0 32.0 37.0 22.0 37.0 7 31.92683622778286 35.0 32.0 37.0 18.0 37.0 8 31.78649309252313 35.0 32.0 37.0 17.0 37.0 9 32.42120120904945 37.0 32.0 39.0 11.0 39.0 10 32.57336492342758 37.0 32.0 39.0 16.0 39.0 11 32.505528766336845 37.0 32.0 39.0 11.0 39.0 12 32.46813263555214 37.0 32.0 39.0 12.0 39.0 13 32.47411875847502 37.0 32.0 39.0 11.0 39.0 14 33.333713071401185 38.0 32.0 40.0 11.0 41.0 15 33.36410725493934 38.0 32.0 40.0 11.0 41.0 16 33.32285818946773 38.0 32.0 40.0 10.0 41.0 17 33.36954540683314 38.0 32.0 40.0 10.0 41.0 18 33.35192636023418 38.0 32.0 41.0 10.0 41.0 19 33.31062930980928 38.0 32.0 41.0 10.0 41.0 20 33.246630663670466 38.0 32.0 41.0 8.0 41.0 21 33.194456881020876 38.0 32.0 41.0 2.0 41.0 22 33.145417544070106 38.0 32.0 41.0 2.0 41.0 23 32.89452650204686 38.0 32.0 41.0 2.0 41.0 24 32.809788502034 38.0 32.0 41.0 2.0 41.0 25 32.65165751596464 38.0 32.0 41.0 2.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.35310052521915836 1101 2 0.4881212806098638 1101 3 -1.4592565343074533 1101 4 -0.42930275347703883 1101 5 0.4391587810308053 1101 6 0.8002172107043037 1101 7 0.9508909091786677 1101 8 0.9578996215163684 1101 9 1.7968118610956267 1101 10 1.4364925354285845 1101 11 1.3305483589137168 1101 12 1.3866269424152833 1101 13 1.188336671995657 1101 14 1.4552052507901152 1101 15 1.3482646857912712 1101 16 1.367894119639331 1101 17 1.3070798238228818 1101 18 1.3922099547252245 1101 19 1.3336788043727665 1101 20 1.5386791333788352 1101 21 1.495633111126665 1101 22 1.5399779269482181 1101 23 1.8295658543950566 1101 24 1.7526287109922478 1101 25 1.8267047352372998 1102 1 0.646306502052898 1102 2 0.6393334283665197 1102 3 0.7487723308013337 1102 4 0.7728487837578655 1102 5 0.8013667777923175 1102 6 1.0361933274623354 1102 7 1.1138077227957268 1102 8 1.0893539160069423 1102 9 1.6628108340862582 1102 10 1.1046265658074503 1102 11 1.139760883658333 1102 12 1.2836906556732828 1102 13 1.2515848100252285 1102 14 1.2201725976097109 1102 15 1.2473317399636485 1102 16 1.2042289864545026 1102 17 1.0333282448227337 1102 18 1.191760213900622 1102 19 1.1904292660148528 1102 20 1.2819272500060066 1102 21 1.5240760576070684 1102 22 1.4201760153165779 1102 23 1.7648351416630916 1102 24 1.7493581187894165 1102 25 1.5517896301431122 1103 1 -2.395564079785057 1103 2 -2.1874995251553493 1103 3 -2.067637599919397 1103 4 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2214 23 -1.6386748141208436 2214 24 -2.0160733603708856 2214 25 -9.51944515810845 2215 1 -0.5627133295876376 2215 2 -0.2185603306304742 2215 3 -0.22412614953771737 2215 4 -0.32979585484584106 2215 5 -0.28934818812484764 2215 6 -0.26247288803955726 2215 7 -0.3422346288663185 2215 8 -0.458794826950939 2215 9 0.33308152293271576 2215 10 0.19216348237961256 2215 11 0.35175586931681835 2215 12 0.2852320160467343 2215 13 0.2005117979091935 2215 14 0.11593170596383118 2215 15 -0.36099110507685594 2215 16 -0.14432507480857737 2215 17 -0.08689664764990113 2215 18 0.11358181745074347 2215 19 -0.06793835913363466 2215 20 -0.0042708150524717325 2215 21 0.0010888400842361534 2215 22 -0.07156796985681524 2215 23 0.2820114656463488 2215 24 0.4367407204041953 2215 25 0.5468217362710419 2216 1 -0.5551466707751302 2216 2 -0.4648752340648308 2216 3 -0.502525757852478 2216 4 -0.40814630151801623 2216 5 -1.1199981207168541 2216 6 -1.390685152174381 2216 7 -0.8698668150968913 2216 8 -1.8193030208595182 2216 9 -0.5237949199511114 2216 10 -0.882218993993753 2216 11 -0.4261337321784282 2216 12 -1.592241935752746 2216 13 -1.308504754182085 2216 14 -1.4848788380762699 2216 15 -1.03052088917363 2216 16 -2.112421259046421 2216 17 -2.462033277555655 2216 18 -1.8958736587361784 2216 19 -3.2465737999614213 2216 20 -2.147389146483004 2216 21 -2.2323020048227065 2216 22 -2.066941362179655 2216 23 -1.752100872257735 2216 24 -1.8593409332117687 2216 25 -1.8921904590723244 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 94132.0 3 4.0 4 1222.0 5 12551.0 6 18854.0 7 14858.0 8 11626.0 9 9058.0 10 7839.0 11 6893.0 12 6156.0 13 5649.0 14 5483.0 15 5553.0 16 5473.0 17 5848.0 18 6832.0 19 9028.0 20 12885.0 21 16807.0 22 20128.0 23 23680.0 24 26604.0 25 29875.0 26 32859.0 27 35374.0 28 38877.0 29 45459.0 30 55165.0 31 68470.0 32 83835.0 33 104160.0 34 129584.0 35 161418.0 36 212490.0 37 313121.0 38 696215.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.91127848548807 19.418537128898507 13.710404234119375 21.959780151494048 2 16.060683050639046 21.79728700885893 39.387728258732004 22.75430168177002 3 18.515489305768227 28.558281656614092 29.85841849959427 23.067810538023412 4 11.620517592861567 18.072818791083645 36.700189120798235 33.606474495256556 5 12.037315227179118 40.20297419470145 32.90858103645504 14.851129541664399 6 28.75803634630975 38.63805033225182 17.915289068512262 14.688624252926164 7 26.723732673967405 34.95392223453058 20.799509760858435 17.522835330643588 8 27.29978872122773 34.55359323907929 19.039474417269147 19.107143622423834 9 27.539635735249746 15.742390007405985 19.734035987601832 36.98393826974244 10 15.876823563800196 32.34819385857914 29.889773984808887 21.88520859281178 11 33.148672326651536 24.599589216768734 22.061725495305826 20.190012961273908 12 23.93322061756018 29.18431599721324 28.426355267205793 18.456108118020783 13 28.83887107741045 23.173221400223564 26.52830125624836 21.459606266117625 14 22.35595658248843 22.79843540653251 29.318945951770793 25.526662059208267 15 24.10001306828285 29.226978934356517 25.709468506509502 20.96353949085113 16 21.420338564741893 29.948214368348978 26.957322058125367 21.67412500878376 17 20.791863695649564 29.039615465696816 28.25691265367404 21.911608184979578 18 19.91706721138177 26.056810288567885 32.88995957915702 21.136162920893327 19 21.576590300694498 25.975657676813512 33.153356293261446 19.294395729230544 20 21.91172746318121 24.974042908582618 33.178840610389095 19.93538901784708 21 22.977314106406837 24.467967953598965 32.733538126124536 19.821179813869662 22 22.00986132696422 25.028076972683174 32.72543098849369 20.236630711858915 23 21.162286386632108 25.053827805012446 33.18277972998045 20.601106078374997 24 21.108341309960743 25.38752698270964 33.129711530304185 20.374420177025428 25 21.015405558765135 24.78442554151489 33.41499536147671 20.785173538243264 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 2742.0 1 2742.0 2 3416.5 3 4091.0 4 4091.0 5 4091.0 6 11066.0 7 18041.0 8 18041.0 9 18041.0 10 18230.5 11 18420.0 12 18420.0 13 18420.0 14 17790.5 15 17161.0 16 17161.0 17 17161.0 18 28766.5 19 40372.0 20 40372.0 21 40372.0 22 61473.0 23 82574.0 24 82574.0 25 82574.0 26 116565.5 27 150557.0 28 150557.0 29 150557.0 30 171626.5 31 192696.0 32 192696.0 33 192696.0 34 204856.5 35 217017.0 36 217017.0 37 217017.0 38 219371.0 39 221725.0 40 221725.0 41 221725.0 42 235564.5 43 249404.0 44 249404.0 45 249404.0 46 265170.0 47 280936.0 48 280936.0 49 280936.0 50 272588.0 51 264240.0 52 264240.0 53 264240.0 54 236883.0 55 209526.0 56 209526.0 57 209526.0 58 181500.5 59 153475.0 60 153475.0 61 153475.0 62 127023.0 63 100571.0 64 100571.0 65 100571.0 66 78062.0 67 55553.0 68 55553.0 69 55553.0 70 41903.0 71 28253.0 72 28253.0 73 28253.0 74 20487.0 75 12721.0 76 12721.0 77 12721.0 78 10309.5 79 7898.0 80 7898.0 81 7898.0 82 5728.0 83 3558.0 84 3558.0 85 3558.0 86 2575.5 87 1593.0 88 1593.0 89 1593.0 90 1238.0 91 883.0 92 883.0 93 883.0 94 470.0 95 57.0 96 57.0 97 57.0 98 29.0 99 1.0 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.027419973308369736 2 0.0015423734985957973 3 1.285311248829831E-4 4 0.004584276787493065 5 0.036931276549710484 6 0.012553206530238017 7 0.021850291230107132 8 0.028062628932784647 9 0.035003309676465734 10 0.0368027454248275 11 0.041172803670848926 12 0.007326274118330038 13 0.002227872831305041 14 0.007197742993447055 15 0.007283430410035711 16 0.009125709866691801 17 0.009811209199401044 18 0.015766484652312597 19 0.018594169399738226 20 0.01739454556749705 21 0.013881361487362177 22 0.012938799904886968 23 0.010025427740872684 24 0.006983524451975416 25 0.10728064556899658 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 2334065.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 39.17920523122475 #Duplication Level Percentage of deduplicated Percentage of total 1 72.30209973088272 28.327388040047342 2 12.675260295001003 9.932132489140773 3 5.456338266128155 6.413249902188599 4 2.781231004118687 4.358656812232454 5 1.6364666873591658 3.2057732099053626 6 1.0744260747256058 2.5257095812472237 7 0.7499752679125807 2.0568404457922838 8 0.5108028189070848 1.6010278779719045 9 0.3965399303244322 1.3982507381300842 >10 2.106353524695611 15.079994043619452 >50 0.17237261218687283 4.713679637046624 >100 0.12703684124016806 9.382849572128048 >500 0.007141599244235598 1.994860421535609 >1k 0.002966510455297863 2.021161826687701 >5k 3.296122728108737E-4 0.7143995415779415 >10k+ 6.592245456217474E-4 6.274025860748672 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 44126 1.8905214721955044 No Hit TATCAACGCAGAGTACTTTTTTTTT 31702 1.3582312403467771 No Hit GGTATCAACGCAGAGTACTTTTTTT 30784 1.318900716132584 No Hit GTACTTTTTTTTTTTTTTTTTTTTT 14345 0.614592995482131 No Hit ACGCAGAGTACTTTTTTTTTTTTTT 14304 0.6128364034420636 No Hit GAGTACTTTTTTTTTTTTTTTTTTT 10489 0.4493876562992033 No Hit GTGGTATCAACGCAGAGTACTTTTT 5898 0.2526921915199448 No Hit GTGGTATCAACGCAGAGTACATGGG 5638 0.24155282736341963 No Hit ATCAACGCAGAGTACTTTTTTTTTT 5060 0.2167891639692982 No Hit GCAGAGTACTTTTTTTTTTTTTTTT 4222 0.18088613641865156 No Hit GGTATCAACGCAGAGTACATGGGAT 3525 0.15102407173750518 No Hit GGTATCAACGCAGAGTACATGGGAG 2766 0.11850569714211044 No Hit GTATAACGCAGAGTACTTTTTTTTT 2742 0.11747744814304657 No Hit GGTATCAACGCAGAGTACATGGGAA 2452 0.10505277273769154 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 0.0 0.0 12 0.0 0.0 0.0 0.0 0.0 13 0.0 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TATCACG 180 0.0 14.777511 2 TATAACG 355 0.0 14.450443 2 TCGACCG 55 1.9639696E-4 13.815563 18 GGTATCA 7195 0.0 13.774073 1 TAACGCA 415 0.0 13.7367525 4 ATAACGC 405 0.0 13.606152 3 GTCGGTT 140 0.0 13.567112 12 GGTCGGT 145 0.0 13.101246 11 GTGGTAT 1955 0.0 12.636751 1 TGGTATC 1950 0.0 12.276702 2 TGGTCGG 155 1.8189894E-12 12.257317 10 TCACGCA 210 0.0 12.215898 4 ATCACGC 210 0.0 12.215374 3 CGAAATC 150 1.4551915E-11 12.031311 13 CATTCCG 250 0.0 11.78004 9 CGACCGT 65 7.9740374E-4 11.701376 19 TAGACTA 130 2.6066118E-9 11.695354 5 TCGACGG 65 8.0373924E-4 11.690343 14 TCCGGTG 260 0.0 11.688589 12 TCGCTCG 90 7.478915E-6 11.609159 17 >>END_MODULE