FastQCFastQC Report
Thu 26 May 2016
SRR1364307_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364307_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1797818
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT98220.5463289387468587No Hit
GTACTTTTTTTTTTTTTTTTTTTTT69980.38924963483511676No Hit
TATCAACGCAGAGTACTTTTTTTTT60390.33590719416537157No Hit
NNNNNNNNNNNNNNNNNNNNNNNNN50060.27844865275572944No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA42370.23567457885058443No Hit
GGTATCAACGCAGAGTACTTTTTTT42260.23506272603789707No Hit
GAGTACTTTTTTTTTTTTTTTTTTT28550.1588036163838609No Hit
ACGCAGAGTACTTTTTTTTTTTTTT20720.11525082071711375No Hit
GAAAAAAAAAAAAAAAAAAAAAAAA18080.10056635321261662No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATAACG2000.015.1918012
TAACGCA2200.014.2578414
GGTATCA24250.013.0383251
TATCACG957.441486E-812.992992
AGGACCG1403.6379788E-1212.899595
CGCATAT905.414404E-712.665148
TACAACG1001.4530633E-712.3433392
TCGCACG701.0832266E-412.2237418
CTGGTCG1251.4133548E-912.1581959
CGTTCGC550.003050387612.10060715
TCGCGCA550.003051026512.10026718
GGGCCGT550.003068960312.0908036
ACGTTCG550.003070244612.0901287
GTATAAC3800.011.9935291
ATAACGC2600.011.6863283
TGACCGA1552.5465852E-1111.65482519
GGACCGA1157.075869E-811.5651166
CCGAGTG752.0770132E-411.3983079
CGAAATC752.0986656E-411.38558713
GGTCGGT1105.0511153E-711.21307811
Produced by FastQC (version 0.11.5)