##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1364307_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1797818
Sequences flagged as poor quality	0
Sequence length	25
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	26.48790311366334	31.0	26.0	31.0	10.0	34.0
2	26.388718435347737	31.0	26.0	31.0	10.0	34.0
3	25.888955389255198	30.0	25.0	31.0	10.0	34.0
4	29.66997938612251	35.0	28.0	37.0	10.0	37.0
5	30.357347072951768	35.0	30.0	37.0	10.0	37.0
6	30.482005965008693	35.0	31.0	37.0	8.0	37.0
7	30.047146040366712	35.0	30.0	37.0	7.0	37.0
8	29.605035659894384	35.0	30.0	37.0	2.0	37.0
9	30.68859139245463	37.0	29.0	39.0	2.0	39.0
10	30.011012238168714	35.0	27.0	39.0	2.0	39.0
11	30.691855905325234	37.0	30.0	39.0	2.0	39.0
12	30.207634476904783	35.0	28.0	39.0	2.0	39.0
13	29.80512599161873	35.0	27.0	39.0	2.0	39.0
14	30.152123852358805	36.0	27.0	40.0	2.0	41.0
15	30.869544080657775	36.0	27.0	40.0	2.0	41.0
16	31.24425108659497	37.0	30.0	40.0	2.0	41.0
17	31.446539082376525	37.0	30.0	40.0	2.0	41.0
18	31.294090948026998	37.0	30.0	40.0	2.0	41.0
19	31.29186992231694	38.0	30.0	40.0	2.0	41.0
20	31.149168603273523	38.0	30.0	40.0	2.0	41.0
21	31.07758126795927	38.0	30.0	40.0	2.0	41.0
22	31.16418458375653	38.0	30.0	40.0	2.0	41.0
23	31.17969115894935	38.0	30.0	40.0	2.0	41.0
24	31.05297811013128	38.0	30.0	40.0	2.0	41.0
25	30.880957916763542	38.0	30.0	40.0	2.0	41.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1101	1	1.165168077166367
1101	2	1.307620060785986
1101	3	2.01264514761057
1101	4	1.493435844706319
1101	5	0.9737948919728154
1101	6	0.9368793600977696
1101	7	1.2167218400781543
1101	8	1.4887273366750584
1101	9	1.3336390164259342
1101	10	1.780793521976964
1101	11	1.0876688029748784
1101	12	1.277244868875691
1101	13	1.3528320707986126
1101	14	2.0348716022618873
1101	15	1.163492062623142
1101	16	0.7132797266891338
1101	17	0.22908556996376817
1101	18	-5.422771160421188
1101	19	-6.74348546493027
1101	20	-7.786215431115885
1101	21	-9.694981340529313
1101	22	-10.059930744094192
1101	23	-11.244439890355082
1101	24	-10.75710491583471
1101	25	-11.042984487119735
1102	1	1.849622073164788
1102	2	2.1267309884484042
1102	3	2.8377733940872965
1102	4	2.257250875027861
1102	5	1.5222512887046982
1102	6	1.6881459392287965
1102	7	1.977984756168354
1102	8	2.275158840748457
1102	9	2.4922038306227243
1102	10	-0.8887557424513304
1102	11	1.1341513760314506
1102	12	2.094193748097336
1102	13	2.6318985133398627
1102	14	3.1155452707118414
1102	15	2.6394940724898532
1102	16	2.3530448915980564
1102	17	2.0652707532306103
1102	18	2.278851470549874
1102	19	2.138105271955002
1102	20	2.3064022333912213
1102	21	2.3577477583786646
1102	22	2.2512284214373395
1102	23	2.264123935995425
1102	24	2.3978975110633307
1102	25	2.4686431311401513
1103	1	-0.47260013240031995
1103	2	-0.2056898681903121
1103	3	-2.955877447155249
1103	4	-0.5304153983643509
1103	5	-3.200205382394426
1103	6	-8.30882429833996
1103	7	-8.552070756994848
1103	8	-2.380965372015524
1103	9	-7.614998189019008
1103	10	-5.1926224748802845
1103	11	-8.2776714435815
1103	12	-2.4039840469372145
1103	13	0.02471223467068384
1103	14	0.12587734526708516
1103	15	-0.1003931022154525
1103	16	0.07411785625282619
1103	17	-0.5725219726410984
1103	18	-0.11308225545825579
1103	19	-6.203962552147875
1103	20	-1.3120230996802604
1103	21	-6.785784425913647
1103	22	-2.7564696637108277
1103	23	-1.694367868954437
1103	24	-7.05573183250813
1103	25	-3.712569943161739
1104	1	1.64821576718127
1104	2	1.7419472572846075
1104	3	2.6033882913144275
1104	4	1.989389944779976
1104	5	1.599772374543008
1104	6	1.36677416027592
1104	7	1.4302835810035184
1104	8	1.948185363809035
1104	9	2.026766767937662
1104	10	2.4065515259853925
1104	11	1.958312421180679
1104	12	2.29158056615373
1104	13	2.437717813471238
1104	14	3.0595554460702417
1104	15	2.1690785949903386
1104	16	1.26043656928125
1104	17	1.2970885934147809
1104	18	1.3240795898743087
1104	19	1.7150727001004746
1104	20	-9.235080138106653
1104	21	-3.2999177881028423
1104	22	-0.6366021001076909
1104	23	0.49968932577456115
1104	24	1.0746545956167175
1104	25	1.464650154948206
1105	1	1.499724621536167
1105	2	1.5907348202703702
1105	3	2.2853094038517625
1105	4	1.8031543333663116
1105	5	1.5352436409878472
1105	6	1.4265022414377242
1105	7	1.70883293006408
1105	8	1.9385353787636106
1105	9	1.7183568183678126
1105	10	2.431614459823855
1105	11	1.8776664977564153
1105	12	2.2363073128273747
1105	13	2.2432683495512293
1105	14	3.0744478406301
1105	15	2.1901017164256444
1105	16	1.683845776314886
1105	17	1.2179962842206287
1105	18	1.7282273815819948
1105	19	1.7726582008087455
1105	20	1.736332217695928
1105	21	1.6494209911883928
1105	22	1.6967710861307737
1105	23	1.7099671433619683
1105	24	1.6263657521454284
1105	25	1.6979376379685256
1106	1	0.9328058111051369
1106	2	1.3572247896384866
1106	3	1.9935901980477375
1106	4	1.5225842279458632
1106	5	0.8912276260534142
1106	6	0.5452238034996952
1106	7	1.105457867030342
1106	8	1.1467152396105185
1106	9	1.5174166434025587
1106	10	-2.2206951544811098
1106	11	0.034820515961985876
1106	12	0.7317679578166114
1106	13	1.6541038554429335
1106	14	0.8592879690839901
1106	15	1.3621690007391507
1106	16	0.9179124616425334
1106	17	-5.475177314260954
1106	18	-1.4649265064552743
1106	19	0.12865964747598468
1106	20	0.7350924439771234
1106	21	0.9567065534599024
1106	22	1.0620687191312967
1106	23	0.37053622754329396
1106	24	0.8593287848764071
1106	25	1.011789423893525
1107	1	0.8198046361220257
1107	2	1.1792353513435003
1107	3	1.842381306027896
1107	4	1.2469739333063607
1107	5	0.6328392455060872
1107	6	0.768310381617674
1107	7	0.716697224495956
1107	8	0.9415468910795113
1107	9	0.9816800709360685
1107	10	-2.3770444004678986
1107	11	-0.132697246786055
1107	12	0.26809215326861136
1107	13	1.1776489924060094
1107	14	1.6842421657714297
1107	15	1.2703317161855736
1107	16	-5.474394421865906
1107	17	-2.018428390014506
1107	18	-0.3407694319792185
1107	19	0.6088241188255843
1107	20	0.970371377607659
1107	21	1.082618479669911
1107	22	0.6508528876138868
1107	23	0.32926290859715124
1107	24	0.42331686681504976
1107	25	0.7345729247123316
1108	1	-0.6703559193481645
1108	2	-3.3258426052326726
1108	3	-0.4132000762027914
1108	4	-0.9588119767403036
1108	5	-1.2244849510195657
1108	6	-0.7705229312920565
1108	7	-0.409344427987655
1108	8	-0.18134797014695536
1108	9	-0.8412372886743391
1108	10	-3.4967638125533576
1108	11	-1.80191550250645
1108	12	-6.128705295790702
1108	13	-1.999488759298714
1108	14	-6.405337192253068
1108	15	-3.1165296166135015
1108	16	-7.962905917113691
1108	17	-3.6437846522484065
1108	18	-2.277918283751177
1108	19	-0.994737887563435
1108	20	-1.0656099571624544
1108	21	-0.4499548362093435
1108	22	-0.6223125053330314
1108	23	-0.72076442013147
1108	24	-0.3714871087207108
1108	25	-0.7646830725333977
1109	1	-9.056931549549702
1109	2	-6.252520679958629
1109	3	-5.394190950895403
1109	4	-4.932081700579385
1109	5	-2.290402024840958
1109	6	-1.4057087505398052
1109	7	-0.9543695907007042
1109	8	-0.4508696410894295
1109	9	-5.3814942727538515
1109	10	-1.4107343023386072
1109	11	-0.9711681099497582
1109	12	-5.6546910586441825
1109	13	-7.976011287429028
1109	14	-8.098838639287994
1109	15	-3.7206702126472315
1109	16	-2.114796258885516
1109	17	-1.7267246380789025
1109	18	-1.1166440975191065
1109	19	-0.8911896720456127
1109	20	-0.5903149417851381
1109	21	-0.43237836858075696
1109	22	-0.6412799556292477
1109	23	-0.954438107834136
1109	24	-0.699938005487148
1109	25	-0.6219022041756368
1110	1	-2.2924921212100493
1110	2	-2.1027535031257507
1110	3	-1.3367185046675338
1110	4	-2.295075243827924
1110	5	-5.198416726307876
1110	6	-3.807140136666206
1110	7	-8.635931486841478
1110	8	-9.566141001900636
1110	9	-11.277886241632306
1110	10	-8.893980325725678
1110	11	-11.534615277474302
1110	12	-6.274527720557202
1110	13	-4.616263699905726
1110	14	-4.104223675607294
1110	15	-9.410045359222703
1110	16	-10.900780423841205
1110	17	-11.72497860025328
1110	18	-8.594604040021046
1110	19	-12.078373239692286
1110	20	-12.818712904052305
1110	21	-13.980848830504481
1110	22	-14.4726700450794
1110	23	-14.803745413753713
1110	24	-15.160651429462371
1110	25	-16.17074263134897
1111	1	-0.523535693801886
1111	2	0.09599254578679606
1111	3	-2.73265734391196
1111	4	-3.085059692295605
1111	5	-0.9102295319461682
1111	6	-0.486166006586334
1111	7	-0.01772273016712589
1111	8	0.28472083339806176
1111	9	-4.1887046633714355
1111	10	-4.148392405452121
1111	11	-1.3633584832261754
1111	12	-5.979269807043995
1111	13	-1.2279605410606713
1111	14	0.020732584597915604
1111	15	0.18033428976240984
1111	16	-0.055149127901099604
1111	17	-0.04644865523486885
1111	18	0.2864151922261051
1111	19	0.366572405730647
1111	20	0.484689166824932
1111	21	0.6772008844275135
1111	22	0.35283330744634966
1111	23	0.10137496349025099
1111	24	0.3888701889201016
1111	25	0.5113549415201959
1112	1	-0.5054543537168286
1112	2	0.17927194015042858
1112	3	-2.6017565779607814
1112	4	-0.8264213529390396
1112	5	-0.6685774855010642
1112	6	-0.41426218359877254
1112	7	0.06982820312250837
1112	8	-3.4515328632173485
1112	9	-0.7410660740610311
1112	10	-3.1160483787254663
1112	11	-0.7588805691117386
1112	12	0.32989126831560256
1112	13	0.7343067599306536
1112	14	1.4334860487740677
1112	15	0.7883820909107726
1112	16	0.512257485248135
1112	17	0.12356780847046878
1112	18	0.38103904732174954
1112	19	0.6103558875223101
1112	20	0.6482546841195393
1112	21	0.5447623977109863
1112	22	0.48892385129484595
1112	23	0.31565807391344336
1112	24	0.49700734411293723
1112	25	0.5152807010741043
1113	1	-1.6339172445347891
1113	2	-1.0688775702223836
1113	3	-3.8575784815017826
1113	4	-2.04788567567995
1113	5	-1.9919692503371778
1113	6	-1.742857673100982
1113	7	-1.6534119183350278
1113	8	-9.026522180334126
1113	9	-9.820180590998312
1113	10	-4.061790758764722
1113	11	-9.388519826892392
1113	12	-7.681195838545367
1113	13	-3.493507778074818
1113	14	-1.8336917298765911
1113	15	-7.9209237418839535
1113	16	-3.782812438537377
1113	17	-2.9139197678422164
1113	18	-2.183271909686045
1113	19	-1.8963922428243372
1113	20	-7.66666814320817
1113	21	-3.549782130569792
1113	22	-2.8435860370675385
1113	23	-2.7374690053803334
1113	24	-8.197956643560385
1113	25	-5.28768622424332
1114	1	-4.665982602383949
1114	2	-4.4660776365184915
1114	3	-1.2487559490653837
1114	4	-1.0851938570690969
1114	5	-1.0831088410991718
1114	6	-1.0946591855511656
1114	7	-0.9600290751683964
1114	8	-0.5701921661759748
1114	9	-0.7774561206180621
1114	10	-3.7110927768631825
1114	11	-1.7342514531321669
1114	12	-4.893350405860264
1114	13	-1.7265711358041216
1114	14	-6.323460077418016
1114	15	-2.6497424632305524
1114	16	-1.6708438842419895
1114	17	-1.3772890615077529
1114	18	-0.9988565982816162
1114	19	-0.8692913678655394
1114	20	-0.6653806128692707
1114	21	-0.5288033453242313
1114	22	-0.4939585781186864
1114	23	-0.922529252436334
1114	24	-0.8496293561573935
1114	25	-0.7396344215135677
1115	1	0.31555840183409245
1115	2	0.47442873109993755
1115	3	1.21256178292683
1115	4	0.08532751703862473
1115	5	-0.25123728853590066
1115	6	-0.7292815898340983
1115	7	-2.567355892170344
1115	8	-0.5814926609845408
1115	9	-0.4283930122468753
1115	10	0.42414813812708374
1115	11	-0.589595994014708
1115	12	0.16836885111572641
1115	13	0.5263236970965934
1115	14	1.1232313972737415
1115	15	0.30470574410234974
1115	16	-0.06704055036788148
1115	17	-0.4254974083604459
1115	18	-0.08803874461516514
1115	19	0.09538254517794087
1115	20	-0.0023168555688179993
1115	21	0.21057043793339147
1115	22	-0.28114526695710396
1115	23	-0.31958270798467936
1115	24	-0.48008948756557146
1115	25	-0.26247368984581954
1116	1	0.25355642302335824
1116	2	0.3387320639023095
1116	3	1.205620037189341
1116	4	0.39677379464110274
1116	5	-0.36916391304385954
1116	6	-1.3943272762312304
1116	7	-4.761587487402107
1116	8	-1.2445138781411451
1116	9	-1.4960676989024648
1116	10	-0.7059387307964364
1116	11	-2.5192129935236203
1116	12	-0.6292207929565912
1116	13	-6.809695430718509
1116	14	-1.8332660664065692
1116	15	-1.5277028495309537
1116	16	-1.0538069178577025
1116	17	-0.852603603228058
1116	18	-0.6045468770975582
1116	19	-0.21938151500782155
1116	20	-0.24331247131922495
1116	21	-0.2509615475029925
1116	22	-0.5555182837303043
1116	23	-0.7157239650728542
1116	24	-0.46857373052584705
1116	25	-0.5352338870327173
1201	1	2.535419909193106
1201	2	2.647351341485109
1201	3	3.310620658858465
1201	4	2.9813601209345073
1201	5	2.3600168824665566
1201	6	2.3300618396610773
1201	7	2.550860589097102
1201	8	2.7592252026174435
1201	9	3.061646979459116
1201	10	3.3045442825656792
1201	11	2.7526741160728747
1201	12	3.1744276041731787
1201	13	3.370533395325282
1201	14	3.9533420032354414
1201	15	3.443784437335438
1201	16	2.960268236287984
1201	17	2.733177316804568
1201	18	2.7413732544325597
1201	19	2.940732429407422
1201	20	2.883411836320338
1201	21	2.9141796749999216
1201	22	2.210302018099476
1201	23	1.5255790251556398
1201	24	2.0669053267600255
1201	25	2.2443124687617093
1202	1	2.300514617755983
1202	2	2.4175938090087428
1202	3	3.291606682119024
1202	4	2.709935921791473
1202	5	2.1033643285659913
1202	6	1.9353804821150007
1202	7	2.221591052267886
1202	8	2.489492835073399
1202	9	2.8284909058686942
1202	10	-0.8772128418307013
1202	11	1.4716026510657372
1202	12	2.363778842015801
1202	13	2.8303183881838834
1202	14	3.555604296644951
1202	15	2.9100987099328677
1202	16	2.529114730200792
1202	17	2.2744592830699553
1202	18	2.3154589002191344
1202	19	2.6008485621694426
1202	20	2.4797187124680597
1202	21	2.802093706150032
1202	22	2.4672053711566733
1202	23	2.1782094407170867
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2211	15	-1.4990985343936885
2211	16	-0.5365233481959102
2211	17	-0.051556411006082214
2211	18	0.28878449703611153
2211	19	0.7355088922608068
2211	20	0.8574765963466326
2211	21	0.8355537709894065
2211	22	0.6510087899600947
2211	23	0.5079294525060654
2211	24	0.7537807376738961
2211	25	0.7944357429234472
2212	1	0.7253648605325118
2212	2	0.7487922334701196
2212	3	1.4373493119793785
2212	4	0.8567684407234104
2212	5	0.372001962401864
2212	6	0.24924730625833647
2212	7	0.6191443743308298
2212	8	0.8465398194724045
2212	9	0.651047653435139
2212	10	1.5603315449752237
2212	11	0.725399894066129
2212	12	0.9109376522178181
2212	13	1.0402754584231104
2212	14	-4.841053087389692
2212	15	-1.293967459215601
2212	16	-0.5581786781655431
2212	17	-0.29615216448027937
2212	18	0.1668337815326737
2212	19	0.5303695513390636
2212	20	0.7784858206869529
2212	21	0.9299412162432041
2212	22	0.7022344734624753
2212	23	0.4924062711131718
2212	24	0.6064673913679357
2212	25	0.6878110937870439
2213	1	0.5175263428171455
2213	2	0.6803200011335662
2213	3	1.4121203459698286
2213	4	0.7560456480528401
2213	5	0.25459569079652766
2213	6	-0.04895917536549632
2213	7	0.28873318401325676
2213	8	-5.262183410137325
2213	9	-1.9432988393189525
2213	10	0.1661935423266172
2213	11	-0.05247498130281869
2213	12	0.3920802661029512
2213	13	-8.002208558684046
2213	14	-2.3599516051230403
2213	15	-1.0136696393262383
2213	16	-0.41756855466280385
2213	17	-0.273466615928335
2213	18	0.17069161267816568
2213	19	0.2987176246261676
2213	20	0.6297873779345231
2213	21	0.6091033539365789
2213	22	0.2314289320669829
2213	23	0.23226474238352424
2213	24	0.27846408645362786
2213	25	0.399363144838798
2214	1	-0.9817314623701812
2214	2	-0.818032688428552
2214	3	-7.153308475737404
2214	4	-2.777724436683645
2214	5	-1.715984077705805
2214	6	-1.1681739412010437
2214	7	-0.8186691606335792
2214	8	-6.718061451767348
2214	9	-2.243510239007417
2214	10	-0.43382924009594603
2214	11	-0.822214449289099
2214	12	-0.47688150887735503
2214	13	-6.4040807793168
2214	14	-1.7684051201506072
2214	15	-1.1691451031869562
2214	16	-1.1894542504803738
2214	17	-1.0377630276084773
2214	18	-1.0116467998991432
2214	19	-0.6542446201312266
2214	20	-0.3557091375910808
2214	21	-0.3506600620845859
2214	22	-0.5279573377263489
2214	23	-0.6091885819074818
2214	24	-0.3517776368158785
2214	25	-0.40927510208027584
2215	1	0.007129976296550211
2215	2	0.21495945259445648
2215	3	-2.841615329529308
2215	4	-0.8151403470446184
2215	5	-0.44672657772348856
2215	6	-0.8752917621918002
2215	7	-0.22474342124602842
2215	8	0.0806822437202861
2215	9	0.17244698580459783
2215	10	0.8529553348048431
2215	11	0.0851941043337412
2215	12	-0.1742525360619176
2215	13	0.4362656240163716
2215	14	0.6920966235378074
2215	15	0.14729190120586466
2215	16	0.05069727003007074
2215	17	-0.09867540461301161
2215	18	0.1498486339715619
2215	19	-0.1403092603938063
2215	20	0.23090319060432662
2215	21	0.4044056533935674
2215	22	0.1859274444363912
2215	23	-0.008861065584721217
2215	24	-0.038989822120793605
2215	25	0.030663143762104994
2216	1	0.2441459997394091
2216	2	0.03492088949741046
2216	3	1.1530511759311999
2216	4	-0.3089065975522516
2216	5	-0.5402930617834549
2216	6	-0.7605690761040051
2216	7	-1.2838977966451814
2216	8	-6.505077466984872
2216	9	-2.28701310556054
2216	10	-0.8040810191499617
2216	11	-1.4806713199782848
2216	12	-0.3477222290231623
2216	13	0.27490092681714273
2216	14	1.1112527177203972
2216	15	0.4572612242916527
2216	16	-0.1297945800717919
2216	17	-0.2322922810442769
2216	18	-0.09722817037190623
2216	19	0.09718616882180697
2216	20	0.2186041997998167
2216	21	0.10130008975897908
2216	22	-0.11248769400150493
2216	23	-0.37672600796334876
2216	24	-0.2384436871086706
2216	25	-0.17378614622867516
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	153392.0
3	6.0
4	883.0
5	6918.0
6	11099.0
7	9594.0
8	8247.0
9	6872.0
10	6151.0
11	5781.0
12	5383.0
13	5257.0
14	5289.0
15	5327.0
16	5326.0
17	5785.0
18	6655.0
19	8874.0
20	12448.0
21	15732.0
22	19636.0
23	22444.0
24	26061.0
25	29367.0
26	32747.0
27	35666.0
28	38653.0
29	45157.0
30	51559.0
31	58239.0
32	72783.0
33	92510.0
34	117548.0
35	147790.0
36	188548.0
37	226401.0
38	307690.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	46.56282684818171	18.323757879238773	12.824783143033509	22.288632129546016
2	13.995990413605805	22.068631795618256	42.04490022358579	21.890477567190153
3	15.596461888154046	29.990722671513353	30.386209267950683	24.026606172381918
4	10.266569760626451	17.723366748054342	38.58680179923703	33.42326169208217
5	11.784986766690132	40.35805021233029	33.59424064917745	14.262722371802136
6	28.16445950107792	38.70663220899559	19.457871926371276	13.671036363555212
7	26.962603211943403	33.957022317996014	22.18151464189543	16.898859828165158
8	25.345924758331122	35.63726652624531	21.272775460670395	17.74403325475317
9	27.45186438332623	17.09163119870909	20.01849095092812	35.43801346703656
10	12.718626707831321	32.51475107096434	33.298067432350074	21.468554788854263
11	32.64783456076616	25.786355827371928	23.117770500657564	18.448039111204352
12	21.823504828371977	28.952823287779005	30.24641519814264	18.97725668570638
13	26.587228524976876	24.466144220565543	27.144824246319608	21.801803008137973
14	20.12979259520467	23.50840622147431	29.95457091779914	26.407230265521882
15	22.677319110238287	30.679204860549326	24.83566634493609	21.807809684276304
16	21.570490520939632	30.823028713080653	25.863141550137	21.743339215842717
17	20.838798474242378	30.104199850369973	27.036672745967604	22.020328929420042
18	20.353412050354493	29.036962484071744	29.515312327746074	21.094313137827687
19	22.03697721659383	28.308732253552638	29.254949087843624	20.39934144200991
20	22.248248796659155	27.920526395232475	29.224047809388576	20.60717699871979
21	23.391177629075685	27.242889135113963	28.804707436629588	20.561225799180765
22	22.534474178120863	27.740694370984336	28.666235948661985	21.058595502232816
23	21.83961527303564	27.845171944388653	28.846823324630112	21.468389457945595
24	21.498696996256967	27.914686311124154	29.17266654652959	21.413950146089284
25	21.55077863058757	27.499545329276444	29.39365335457734	21.556022685558656
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	9980.0
1	9980.0
2	8776.0
3	7572.0
4	7572.0
5	7572.0
6	12435.0
7	17298.0
8	17298.0
9	17298.0
10	16804.5
11	16311.0
12	16311.0
13	16311.0
14	18344.5
15	20378.0
16	20378.0
17	20378.0
18	28409.5
19	36441.0
20	36441.0
21	36441.0
22	49140.0
23	61839.0
24	61839.0
25	61839.0
26	81629.0
27	101419.0
28	101419.0
29	101419.0
30	119276.5
31	137134.0
32	137134.0
33	137134.0
34	148023.0
35	158912.0
36	158912.0
37	158912.0
38	170891.0
39	182870.0
40	182870.0
41	182870.0
42	194550.0
43	206230.0
44	206230.0
45	206230.0
46	212773.0
47	219316.0
48	219316.0
49	219316.0
50	210518.0
51	201720.0
52	201720.0
53	201720.0
54	181631.5
55	161543.0
56	161543.0
57	161543.0
58	138653.0
59	115763.0
60	115763.0
61	115763.0
62	94032.0
63	72301.0
64	72301.0
65	72301.0
66	55202.0
67	38103.0
68	38103.0
69	38103.0
70	28226.5
71	18350.0
72	18350.0
73	18350.0
74	13046.0
75	7742.0
76	7742.0
77	7742.0
78	5848.5
79	3955.0
80	3955.0
81	3955.0
82	2727.0
83	1499.0
84	1499.0
85	1499.0
86	1102.0
87	705.0
88	705.0
89	705.0
90	542.0
91	379.0
92	379.0
93	379.0
94	207.5
95	36.0
96	36.0
97	36.0
98	29.0
99	22.0
100	22.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.3384102283990927
2	0.33996767192229693
3	0.3534284338014193
4	0.3555421071543393
5	0.3618275042301279
6	0.3044802087864289
7	0.3132686400959385
8	0.3110993437600469
9	0.3109324748111322
10	0.4232352774307522
11	0.31121058972599003
12	0.31143308165787636
13	0.3048139466842584
14	0.3100425070835869
15	0.3019215515697362
16	0.3058707833607184
17	0.30019723909761725
18	0.30069784594436144
19	0.3013096987570488
20	0.5032767499268558
21	0.3014765677059636
22	0.3038127329907699
23	0.2991404024211572
24	0.30153219068893516
25	0.29546928554503293
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1797818.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	62.31948497037163
#Duplication Level	Percentage of deduplicated	Percentage of total
1	84.48290023840983	52.64930831660987
2	8.583096213453999	10.697882709472003
3	2.8076818166268454	5.249198543185873
4	1.313315519158441	3.2738058703020108
5	0.7085091636952997	2.2076963089139903
6	0.4409078804469092	1.648629121729774
7	0.29022148844683515	1.2660517581139
8	0.20862913420732848	1.0401328154892202
9	0.1507432001577743	0.8454814736956322
>10	0.898625077271917	10.203679040515325
>50	0.07442402966337819	3.1903444509793757
>100	0.0379170455252321	4.189865853416274
>500	0.0018358745067273167	0.7680393897860089
>1k	8.261435280272926E-4	1.1758565214547454
>5k	3.671749013454634E-4	1.5940278263360002
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	9822	0.5463289387468587	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	6998	0.38924963483511676	No Hit
TATCAACGCAGAGTACTTTTTTTTT	6039	0.33590719416537157	No Hit
NNNNNNNNNNNNNNNNNNNNNNNNN	5006	0.27844865275572944	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA	4237	0.23567457885058443	No Hit
GGTATCAACGCAGAGTACTTTTTTT	4226	0.23506272603789707	No Hit
GAGTACTTTTTTTTTTTTTTTTTTT	2855	0.1588036163838609	No Hit
ACGCAGAGTACTTTTTTTTTTTTTT	2072	0.11525082071711375	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAA	1808	0.10056635321261662	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATAACG	200	0.0	15.191801	2
TAACGCA	220	0.0	14.257841	4
GGTATCA	2425	0.0	13.038325	1
TATCACG	95	7.441486E-8	12.99299	2
AGGACCG	140	3.6379788E-12	12.89959	5
CGCATAT	90	5.414404E-7	12.66514	8
TACAACG	100	1.4530633E-7	12.343339	2
TCGCACG	70	1.0832266E-4	12.22374	18
CTGGTCG	125	1.4133548E-9	12.158195	9
CGTTCGC	55	0.0030503876	12.100607	15
TCGCGCA	55	0.0030510265	12.100267	18
GGGCCGT	55	0.0030689603	12.090803	6
ACGTTCG	55	0.0030702446	12.090128	7
GTATAAC	380	0.0	11.993529	1
ATAACGC	260	0.0	11.686328	3
TGACCGA	155	2.5465852E-11	11.654825	19
GGACCGA	115	7.075869E-8	11.565116	6
CCGAGTG	75	2.0770132E-4	11.398307	9
CGAAATC	75	2.0986656E-4	11.385587	13
GGTCGGT	110	5.0511153E-7	11.213078	11
>>END_MODULE