FastQCFastQC Report
Thu 26 May 2016
SRR1364307_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364307_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1797818
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT208211.1581261284512672No Hit
GTACTTTTTTTTTTTTTTTTTTTTT177050.9848049135118238No Hit
TATCAACGCAGAGTACTTTTTTTTT153310.8527559519372929No Hit
GGTATCAACGCAGAGTACTTTTTTT137880.766929689212145No Hit
GAGTACTTTTTTTTTTTTTTTTTTT83520.4645631537786361No Hit
ACGCAGAGTACTTTTTTTTTTTTTT61800.34375003476436433No Hit
GTGGTATCAACGCAGAGTACTTTTT27440.1526294652740155No Hit
GTATAACGCAGAGTACTTTTTTTTT27030.15034892297218072No Hit
GTGGTATCAACGCAGAGTACATGGG26930.1497926931424649No Hit
GCAGAGTACTTTTTTTTTTTTTTTT23230.12921218944298032No Hit
ATCAACGCAGAGTACTTTTTTTTTT22550.1254298266009129No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATAACG3700.015.1476282
CTGTGCG602.5669637E-514.2510229
AGTCCGG602.5676374E-514.25062610
TAACGCA4200.013.57391554
ATAACGC4000.013.3009553
GGTATCA33600.013.1508281
TATCACG2050.012.9747342
AGGACCG1051.9843355E-812.67110355
CGGTTTG604.0997152E-412.66405114
CGCGGTT604.1026028E-412.66299318
ATCACGC2350.012.5328143
TCACGCA2200.012.525024
GTATAAC5400.012.3180981
GTACTAG701.088371E-412.2175431
GCCGACC701.0900691E-412.2155038
GGACCGA1209.993528E-911.8768436
CAGTCCG802.8648401E-511.8758539
GTAGGAC1052.718043E-711.7627493
TGGTCGG658.0215343E-411.692821510
CGAGTGG907.4586133E-611.61162210
Produced by FastQC (version 0.11.5)