FastQCFastQC Report
Thu 26 May 2016
SRR1364306_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364306_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1846542
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT71600.38775180851559293No Hit
NNNNNNNNNNNNNNNNNNNNNNNNN52010.28166161397899425No Hit
TATCAACGCAGAGTACTTTTTTTTT43890.23768752619761696No Hit
GGTATCAACGCAGAGTACTTTTTTT35310.19122229551236852No Hit
GTGGTATCAACGCAGAGTACATGGG19530.10576526285348506No Hit
GTACTTTTTTTTTTTTTTTTTTTTT19270.10435722555999269No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCGCGC2750.011.7511919
GGACCGA658.010109E-411.6948356
TGACCGA1157.0278475E-811.57086619
TTGACCG1406.7484507E-1011.54166118
ATTTGCG2650.011.47792416
TTGCGCG2650.011.477612518
AGCACCG752.06326E-411.4064985
CGCGGTT752.0643033E-411.40587718
CGAGTGG752.0716185E-411.40153310
TTCTACG752.0863177E-411.3928582
GGTATCA23800.011.2894481
AAACGGC600.005855035511.08874619
TTTGCGC2750.011.05994417
TCTTATA1408.434654E-910.8503412
CGCAGAT5250.010.6782088
GTCTAGA2050.010.6521491
TATAACG1252.2597669E-710.6333342
GTTATCA4150.010.5238091
TATACGC1451.4575562E-810.4761913
GTCTAGG2001.8189894E-1210.4437371
Produced by FastQC (version 0.11.5)