FastQCFastQC Report
Thu 26 May 2016
SRR1364306_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364306_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1846542
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT150640.8157951457372754No Hit
GGTATCAACGCAGAGTACTTTTTTT122700.664485291967364No Hit
TATCAACGCAGAGTACTTTTTTTTT112220.6077305579835173No Hit
ACGCAGAGTACTTTTTTTTTTTTTT49570.2684477255323735No Hit
GTACTTTTTTTTTTTTTTTTTTTTT43910.23779583675865484No Hit
GTGGTATCAACGCAGAGTACATGGG34260.18553599105788007No Hit
GAGTACTTTTTTTTTTTTTTTTTTT32890.17811671762678563No Hit
GTGGTATCAACGCAGAGTACTTTTT22980.1244488346325185No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTCCGC350.002173364616.28239416
GGTCGGT951.8189894E-1115.99977311
GGACCGA704.4311855E-714.9303816
AGGACCG1203.6379788E-1214.2528865
CGAGTGG602.5678082E-514.25056910
TAACGCA2050.013.4391984
ACCGAGT801.9984764E-613.0633758
TGGTCGG1102.746674E-912.95506310
TATAACG2250.012.6654572
CGGTTTG1207.4396667E-1012.66408414
GTCGGTT1207.4396667E-1012.66305512
GGTATCA38300.012.4046011
ATAACGC2150.012.3715813
CTGGTCG1251.4097168E-912.1611439
CGTGCGC550.003067902812.091392510
TCCGCCG550.00307413312.08811818
CCGAGCG658.0183387E-411.6934099
CGAAATC752.0781478E-411.39767613
GTGGTAT10650.011.3309331
AACTTCG600.005871066411.0846777
Produced by FastQC (version 0.11.5)