FastQCFastQC Report
Thu 26 May 2016
SRR1364305_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364305_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6273049
Sequences flagged as poor quality0
Sequence length25
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNN162750.25944321493423694No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGACCGA5850.013.1543726
AGGACCG8100.012.5547085
GGTATCA28450.011.8457881
ACCGAGT5600.011.5358988
TCGCGGT2850.011.34186517
CTTCGCG2900.011.14631615
CGAGTGG5650.010.92920310
ACGGCCC5000.010.83815315
TGGACCG2550.010.8085065
CGCGGTT3200.010.69520318
CGAGCGA2850.010.66666710
TTCGCGG3100.010.42728216
GGACCGT2400.010.2920786
CATGGGT34000.010.230814
GTTTACG951.6573582E-49.9929631
GTATCAA62550.09.9865721
TGCCGTA3000.09.82405715
CGCGCCA2450.09.69387810
CAGGACC18250.09.6862994
CGCCCTA1502.6251837E-79.50684718
Produced by FastQC (version 0.11.5)