##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1364305_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	6273049
Sequences flagged as poor quality	0
Sequence length	25
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	27.027650031109275	31.0	27.0	31.0	10.0	34.0
2	26.9382963531769	31.0	27.0	31.0	10.0	34.0
3	26.318056020286146	31.0	25.0	31.0	10.0	34.0
4	30.258928951455665	35.0	30.0	37.0	10.0	37.0
5	30.77046664229787	35.0	31.0	37.0	16.0	37.0
6	31.140654249632036	35.0	32.0	37.0	17.0	37.0
7	30.78506273424614	35.0	31.0	37.0	10.0	37.0
8	30.330586609478104	35.0	30.0	37.0	10.0	37.0
9	31.59621565206967	37.0	30.0	39.0	10.0	39.0
10	30.841044602074685	35.0	29.0	39.0	10.0	39.0
11	31.58621939666022	37.0	31.0	39.0	10.0	39.0
12	31.052785176713908	37.0	30.0	39.0	8.0	39.0
13	30.711848576346206	35.0	29.0	39.0	8.0	39.0
14	31.066125420030993	36.0	27.0	40.0	8.0	41.0
15	31.798470090063063	37.0	30.0	40.0	2.0	41.0
16	32.17925716824466	37.0	31.0	40.0	2.0	41.0
17	32.33947112480709	38.0	31.0	40.0	2.0	41.0
18	32.16731034621282	38.0	31.0	40.0	2.0	41.0
19	32.12904825069914	38.0	31.0	40.0	2.0	41.0
20	31.97109619261702	38.0	31.0	40.0	2.0	41.0
21	31.899918683880838	38.0	31.0	40.0	2.0	41.0
22	31.984412205292834	38.0	31.0	40.0	2.0	41.0
23	31.99458046637289	38.0	31.0	40.0	2.0	41.0
24	31.82084915963513	38.0	31.0	40.0	2.0	41.0
25	31.63623430966345	38.0	31.0	40.0	2.0	41.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1101	1	0.7354664664063684
1101	2	0.8650049023301065
1101	3	1.6047787955036235
1101	4	0.9487026871598196
1101	5	0.4154430156295099
1101	6	0.25812241803847513
1101	7	0.6179063632643675
1101	8	0.8913730067019685
1101	9	0.8334682748890394
1101	10	1.1880913193110274
1101	11	0.5987092211680043
1101	12	0.9910036894203564
1101	13	0.9288735718800467
1101	14	1.4480478909896632
1101	15	0.669717498000086
1101	16	0.23586724722092356
1101	17	-0.3460444936995657
1101	18	-6.254411265585439
1101	19	-7.748150108076821
1101	20	-8.804987564455224
1101	21	-10.563959831453776
1101	22	-10.896583913693757
1101	23	-12.201747362696
1101	24	-11.978522951738007
1101	25	-12.455893707177193
1102	1	1.9175458926881248
1102	2	2.060133509381881
1102	3	2.865575288161171
1102	4	2.264051300222544
1102	5	1.600015872859366
1102	6	1.6190643012448511
1102	7	1.9315730404961506
1102	8	2.2402417365547116
1102	9	2.399112775520411
1102	10	-1.2747826180254869
1102	11	0.9733030763440205
1102	12	2.0209759684551116
1102	13	2.5799177227976102
1102	14	3.2281102096916925
1102	15	2.7517992090947665
1102	16	2.409633476156941
1102	17	2.004353793858911
1102	18	2.249284891745745
1102	19	1.949548849095052
1102	20	2.3176673563833106
1102	21	2.496734269803465
1102	22	2.2867166849701768
1102	23	2.293047386803895
1102	24	2.517679663410764
1102	25	2.6077531282581283
1103	1	-0.6439518325235696
1103	2	0.02735733581147315
1103	3	-3.28750334570989
1103	4	-0.7851464973079061
1103	5	-3.678129188251443
1103	6	-7.775485705271265
1103	7	-8.550444393882966
1103	8	-2.52922394872569
1103	9	-7.5844436550527945
1103	10	-4.803442159655585
1103	11	-9.121804975256413
1103	12	-2.567810438230179
1103	13	-0.10428397202265316
1103	14	0.3819015647983761
1103	15	0.01279281879993377
1103	16	-0.11806969328614514
1103	17	-0.09516495893632282
1103	18	0.049293827489648834
1103	19	-6.6378311345235055
1103	20	-1.8363717011455485
1103	21	-7.502249373733733
1103	22	-2.917549018586854
1103	23	-1.916105403387764
1103	24	-7.812631460284912
1103	25	-3.8410073580174036
1104	1	1.6097813444248885
1104	2	1.6900621745697784
1104	3	2.5164529642379456
1104	4	2.0191196615558304
1104	5	1.5814555487083979
1104	6	1.4450700376666
1104	7	1.6570502687323234
1104	8	1.9613352009693905
1104	9	2.1407933020185617
1104	10	2.3802718245012464
1104	11	1.943311287532051
1104	12	2.2620290963912026
1104	13	2.5576223056812424
1104	14	3.075632510473305
1104	15	2.1258332462920713
1104	16	1.3071383227221176
1104	17	1.11928628609963
1104	18	1.123861950156396
1104	19	1.5994296881411785
1104	20	-9.497892022728976
1104	21	-3.348887936441269
1104	22	-0.84190986203987
1104	23	0.2230195828220367
1104	24	0.8534759576946094
1104	25	1.3423991485162396
1105	1	1.2387807553620576
1105	2	1.1797203005800263
1105	3	2.0540529094319986
1105	4	1.4109572821353886
1105	5	1.1613969621125477
1105	6	1.1063530759444795
1105	7	1.4430084521578301
1105	8	1.6988252920842726
1105	9	1.6431226705483049
1105	10	2.157858917759949
1105	11	1.7122872303037262
1105	12	1.940339043095456
1105	13	1.962737858639521
1105	14	2.7161525212668565
1105	15	2.022384727879544
1105	16	1.3785464541394603
1105	17	0.7122544520036129
1105	18	1.2511653651518628
1105	19	1.2885110860272277
1105	20	1.222208647605612
1105	21	1.1539319983749543
1105	22	1.12296657955142
1105	23	1.070081889141914
1105	24	1.1099635725806571
1105	25	1.1728916171317572
1106	1	1.1344587090351723
1106	2	1.4275666316609446
1106	3	1.9307616380145376
1106	4	1.5994368043547524
1106	5	0.8416208719658265
1106	6	0.6380320367823771
1106	7	1.2083148540964999
1106	8	1.2747097722681282
1106	9	1.5152102203311095
1106	10	-2.1243162220460654
1106	11	0.0985441393003903
1106	12	0.7273809735778656
1106	13	1.7912137023468944
1106	14	0.8231892983258717
1106	15	1.4133852581249435
1106	16	1.0534065016612004
1106	17	-6.133336917442641
1106	18	-1.718488781809139
1106	19	-0.15744029607786558
1106	20	0.6579368068522555
1106	21	0.9981746406720937
1106	22	1.0408119298526053
1106	23	0.5567288414303668
1106	24	0.8682509528448712
1106	25	0.8900595131300229
1107	1	0.9781775392477883
1107	2	1.312792174791639
1107	3	1.754690317811459
1107	4	1.3519034737997977
1107	5	0.6864351826780997
1107	6	0.9347901180909197
1107	7	0.9713299829318949
1107	8	1.2062238102201484
1107	9	1.3134765658359875
1107	10	-2.223508384000528
1107	11	0.19061661965053744
1107	12	0.5891346884644939
1107	13	1.5570328549792904
1107	14	1.9321858097272724
1107	15	1.623574447734196
1107	16	-5.5123448695700645
1107	17	-1.75074050106047
1107	18	0.09274015358685261
1107	19	0.9710623491414623
1107	20	1.4064311163050576
1107	21	1.5178484769592124
1107	22	1.1327874632468102
1107	23	0.8658313818860428
1107	24	1.0755640564813156
1107	25	1.3398546543618899
1108	1	-0.43309949036271433
1108	2	-3.2639969817797656
1108	3	-0.18156048544177494
1108	4	-0.7385484709959904
1108	5	-0.9401818750871413
1108	6	-0.6191513415979983
1108	7	-0.190458452259616
1108	8	0.053715454294067655
1108	9	-0.40955936493100964
1108	10	-3.5340900553586785
1108	11	-1.5859776339061291
1108	12	-6.2711238836066805
1108	13	-1.9071436239855224
1108	14	-6.29229890252957
1108	15	-2.936177950876573
1108	16	-8.087492608693925
1108	17	-3.542902216830573
1108	18	-2.1311397368153706
1108	19	-0.7272746713452243
1108	20	-0.7218452004017273
1108	21	-0.1678809697275554
1108	22	-0.36367124777185467
1108	23	-0.4114916043249366
1108	24	-0.12634037365801376
1108	25	-0.4046211085036049
1109	1	-9.325829982595081
1109	2	-6.577493510003677
1109	3	-5.452283662315274
1109	4	-5.391604574110378
1109	5	-2.6233244111425513
1109	6	-1.5014226793220757
1109	7	-1.0614939100940681
1109	8	-0.4750202242687429
1109	9	-5.7398483825838404
1109	10	-1.3862545956868928
1109	11	-0.9770822040437785
1109	12	-6.24879060844145
1109	13	-8.093367060803438
1109	14	-8.328543007214794
1109	15	-3.8889934457973006
1109	16	-2.152745842428182
1109	17	-1.758731135688091
1109	18	-1.130283924024571
1109	19	-0.811592374455536
1109	20	-0.5463811173371695
1109	21	-0.4734425982147492
1109	22	-0.709068215155817
1109	23	-0.9439389549925892
1109	24	-0.530272243146996
1109	25	-0.4998736494460623
1110	1	-2.4679789815897593
1110	2	-2.322406634131518
1110	3	-1.62480000764236
1110	4	-2.4001119314806303
1110	5	-5.659852402761185
1110	6	-4.123521577285906
1110	7	-9.47654792434702
1110	8	-10.352190960448215
1110	9	-11.952862438816279
1110	10	-9.551713536687888
1110	11	-12.502013932073972
1110	12	-6.969930116030664
1110	13	-4.985752857472441
1110	14	-4.572136380182144
1110	15	-10.502328922349921
1110	16	-12.21064142554349
1110	17	-12.911422409935504
1110	18	-9.380544943427505
1110	19	-13.114613839307363
1110	20	-14.122993112736495
1110	21	-14.999717855069026
1110	22	-15.766557650344264
1110	23	-16.37154509289165
1110	24	-16.733040800856493
1110	25	-17.505946147824922
1111	1	-0.45819961491996963
1111	2	0.3390576090715314
1111	3	-2.552561913655449
1111	4	-3.237976685987153
1111	5	-0.9431311833931133
1111	6	-0.3657206132180342
1111	7	0.10586195880687299
1111	8	0.5048009032822023
1111	9	-4.221021975348112
1111	10	-4.132701356677305
1111	11	-1.1098057270262416
1111	12	-6.057155688524027
1111	13	-1.1736218812684704
1111	14	0.28630996140995535
1111	15	0.40625633442850173
1111	16	0.24850150397886495
1111	17	0.3282190853192617
1111	18	0.5824162538323563
1111	19	0.6612072284339661
1111	20	0.7190479007943367
1111	21	0.9131264882608257
1111	22	0.3926676161206508
1111	23	0.2457894143533359
1111	24	0.49814855457830376
1111	25	0.7136769092759003
1112	1	-0.11808451663799602
1112	2	0.3888355598586628
1112	3	-2.3995520811235913
1112	4	-0.36185169862746136
1112	5	-0.3997463529864369
1112	6	-0.23742724077708743
1112	7	0.22865757807232967
1112	8	-3.5505786327774906
1112	9	-0.7531482404344381
1112	10	-3.3010263878740496
1112	11	-0.7668939871508158
1112	12	0.4539565920097033
1112	13	0.9453860107349001
1112	14	1.598699475547761
1112	15	0.9356359334210644
1112	16	0.6840782106001555
1112	17	0.29654214642598475
1112	18	0.44954448997643226
1112	19	0.616882535717842
1112	20	0.6941021299669217
1112	21	0.5920245922610654
1112	22	0.5336249390914283
1112	23	0.30061989223269237
1112	24	0.5091497450566429
1112	25	0.5396193745184057
1113	1	-1.0231159204413984
1113	2	-0.5082629650345822
1113	3	-3.15476505345228
1113	4	-1.3899477964295244
1113	5	-1.4792573024977251
1113	6	-1.3024642889361182
1113	7	-1.0576863222181885
1113	8	-8.95101680068441
1113	9	-10.06221319790534
1113	10	-3.6502061958704033
1113	11	-9.653172469761298
1113	12	-7.836483056977595
1113	13	-2.9551116042753094
1113	14	-1.1777107027092555
1113	15	-7.374401768549596
1113	16	-3.0267664534646705
1113	17	-2.138373043844151
1113	18	-1.4861048651723685
1113	19	-1.1607890409924906
1113	20	-7.134986641645337
1113	21	-2.7009697910451536
1113	22	-1.9407447321802387
1113	23	-1.8580909859968493
1113	24	-7.877817499269916
1113	25	-4.624308454902767
1114	1	-4.501177446101487
1114	2	-4.382512064159524
1114	3	-1.0296920240613687
1114	4	-0.6581498287292504
1114	5	-0.7428533711884597
1114	6	-0.7485203867774182
1114	7	-0.6074863536140853
1114	8	-0.26614646083502436
1114	9	-0.5989972001261954
1114	10	-3.6782520871973396
1114	11	-1.570367485604958
1114	12	-5.054288675171126
1114	13	-1.5380298245047506
1114	14	-6.348258557491533
1114	15	-2.4652435754531936
1114	16	-1.425348342805826
1114	17	-1.2537964998030446
1114	18	-0.8404091779399749
1114	19	-0.5723254339697732
1114	20	-0.393039716707527
1114	21	-0.20182260590066292
1114	22	-0.3351742686557344
1114	23	-0.7988566448220737
1114	24	-0.6042523848018178
1114	25	-0.47104015829501833
1115	1	0.6930026176384274
1115	2	0.7883692305636565
1115	3	1.5523883571118908
1115	4	0.44107801193547047
1115	5	0.19288393969842232
1115	6	-0.41406909746175913
1115	7	-2.51378479007575
1115	8	-0.27372781087661124
1115	9	-0.09278455573553757
1115	10	0.7157284588491066
1115	11	-0.20775324821911667
1115	12	0.5585250588045412
1115	13	0.7909951230344667
1115	14	1.4449628098795984
1115	15	0.5498728309076917
1115	16	0.25050506652058147
1115	17	-0.24126525154049716
1115	18	0.09650287691844994
1115	19	0.3358793330826373
1115	20	0.35495891915288524
1115	21	0.4452164050306031
1115	22	-0.08950161895232966
1115	23	-0.03586010915800841
1115	24	-0.15111059121687376
1115	25	0.0880501426272211
1116	1	0.6940228269424686
1116	2	0.815235467127394
1116	3	1.7054143291391135
1116	4	1.0720773878832546
1116	5	0.08997865147815887
1116	6	-0.9181280569551689
1116	7	-4.640942726116187
1116	8	-0.7857270998899217
1116	9	-0.90353096127809
1116	10	-0.10389610999600052
1116	11	-1.9972716076634462
1116	12	-0.18577796908800792
1116	13	-6.763237236407452
1116	14	-1.5253585788459283
1116	15	-1.120923616192055
1116	16	-0.620542683561311
1116	17	-0.32833900734392785
1116	18	-0.10044997289467261
1116	19	0.18355226914481904
1116	20	0.23948287484009256
1116	21	0.3866481953477994
1116	22	0.12333021304520386
1116	23	0.03977544916854825
1116	24	0.2057055823049616
1116	25	0.21470889977658558
1201	1	2.178522014749646
1201	2	2.3704655731571727
1201	3	3.069138935742263
1201	4	2.565544219388517
1201	5	2.018876002227593
1201	6	1.8687587060578856
1201	7	2.127903845402667
1201	8	2.2580978331778887
1201	9	2.656381941525485
1201	10	2.9500723665098185
1201	11	2.4835937091027027
1201	12	2.8141236383015027
1201	13	2.9831414473333737
1201	14	3.583051148498207
1201	15	3.056177147435445
1201	16	2.5539514394034057
1201	17	2.2851694884618254
1201	18	2.381469715051793
1201	19	2.383678027290138
1201	20	2.600497319653236
1201	21	2.456041290998588
1201	22	1.486624485232472
1201	23	1.048477360788592
1201	24	1.555952446915665
1201	25	1.6357749334344653
1202	1	2.060334795509597
1202	2	2.252811131027869
1202	3	3.0114684777631417
1202	4	2.50476992909989
1202	5	1.956576241020933
1202	6	1.754845214155754
1202	7	2.1080262976230593
1202	8	2.4269293769026383
1202	9	2.5672128063789152
1202	10	-1.2167010145277786
1202	11	1.1512133537169156
1202	12	2.2382586494384284
1202	13	2.8124162049047428
1202	14	3.416174002709674
1202	15	2.896736852389811
1202	16	2.4796868460731716
1202	17	2.111326567044685
1202	18	2.2967045172308076
1202	19	2.486515189416899
1202	20	2.4106427408258426
1202	21	2.5560933931287444
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2211	14	-5.767929587725046
2211	15	-1.7145952064980996
2211	16	-0.49534398325086215
2211	17	-0.008080997817430102
2211	18	0.470798489009141
2211	19	0.7533922808572058
2211	20	0.956130595552203
2211	21	0.9730003272896148
2211	22	0.8569605881855828
2211	23	0.691268160483876
2211	24	0.9029146053478989
2211	25	0.91439301369115
2212	1	0.8234275335025281
2212	2	0.8480228432533004
2212	3	1.5655413737271786
2212	4	1.0791011860251842
2212	5	0.6811989205833413
2212	6	0.4979469879497529
2212	7	0.7568345483015513
2212	8	1.0522536693068716
2212	9	0.9642291337700435
2212	10	1.6715869778486159
2212	11	0.7984026540631852
2212	12	1.1367385850858156
2212	13	1.2573341692185096
2212	14	-4.798099753021198
2212	15	-1.3398826544384903
2212	16	-0.5230860291144914
2212	17	-0.13354057088808702
2212	18	0.22909843506608496
2212	19	0.6526139462015053
2212	20	0.9686376391146112
2212	21	0.8877240302712401
2212	22	0.8407456501483885
2212	23	0.7130109643592029
2212	24	0.7550646744818188
2212	25	0.7927091841267249
2213	1	0.7519584420435628
2213	2	0.8472539801103807
2213	3	1.5170252786595917
2213	4	1.0423138709135742
2213	5	0.4808576786411436
2213	6	0.14448742117150815
2213	7	0.32560530069905624
2213	8	-5.299740969093161
2213	9	-1.646172323494909
2213	10	0.22963166469346774
2213	11	0.08600523293303297
2213	12	0.6579380843960152
2213	13	-8.720164201952809
2213	14	-2.6301460658944507
2213	15	-0.8862525787002902
2213	16	-0.3288309405416818
2213	17	-0.09479915607536071
2213	18	0.0884634345713664
2213	19	0.5259659916182855
2213	20	0.6440233401456723
2213	21	0.6459260460984133
2213	22	0.28560776443335456
2213	23	0.2919803125103648
2213	24	0.3987660453115076
2213	25	0.546070115760724
2214	1	-1.0399127327281619
2214	2	-0.7724296746053341
2214	3	-7.346284619528806
2214	4	-2.893494758276308
2214	5	-1.8241117778774267
2214	6	-1.2727961635304936
2214	7	-0.8869801306145781
2214	8	-6.90491778288586
2214	9	-2.4534630846022942
2214	10	-0.5500566576029797
2214	11	-0.870491280967606
2214	12	-0.4596830830826164
2214	13	-6.792592919243276
2214	14	-1.9094909709383536
2214	15	-1.2368016612289523
2214	16	-1.150203092588125
2214	17	-0.9606041095454181
2214	18	-1.0031511399494768
2214	19	-0.5355601905923955
2214	20	-0.34260194063848104
2214	21	-0.34556028664174576
2214	22	-0.5770096981530664
2214	23	-0.6243689392181011
2214	24	-0.437857193406078
2214	25	-0.41197883549223135
2215	1	0.08078881951411887
2215	2	0.3446596947969809
2215	3	-2.903817227540433
2215	4	-0.8345076803697289
2215	5	-0.455694319337244
2215	6	-0.9338441841336511
2215	7	-0.2467330352062369
2215	8	0.17428737597764155
2215	9	0.42126083522207125
2215	10	0.9494452237768805
2215	11	0.1703965545965893
2215	12	-0.06729520417960089
2215	13	0.6563169918112095
2215	14	0.7002140472223921
2215	15	0.35200211364189116
2215	16	0.022025513172259537
2215	17	-0.17893181041492312
2215	18	0.02027227560096634
2215	19	-0.01115142831356053
2215	20	0.21064645493454748
2215	21	0.32515613378521024
2215	22	0.16128490847884436
2215	23	0.0016205621004701243
2215	24	-0.10659324097666811
2215	25	-0.006655023243137492
2216	1	0.2307214463969096
2216	2	-0.005035662650605843
2216	3	1.2077648105520638
2216	4	-0.24601141077474153
2216	5	-0.41110617437735186
2216	6	-0.7077226771208736
2216	7	-1.2865772750104085
2216	8	-6.792082767909072
2216	9	-2.3003018916220235
2216	10	-0.9121008268918835
2216	11	-1.5138333704188724
2216	12	-0.32101789109931644
2216	13	0.48120573751897666
2216	14	1.165708560842262
2216	15	0.664243901146147
2216	16	-0.15834007791398363
2216	17	-0.18521543849624322
2216	18	-0.11100296542648636
2216	19	0.13530830296068785
2216	20	0.19462667237285558
2216	21	-0.03554858115708015
2216	22	-0.29365536022507044
2216	23	-0.5976597203700429
2216	24	-0.5407333066510773
2216	25	-0.4892017871436849
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	445103.0
3	10.0
4	3193.0
5	19353.0
6	24820.0
7	23235.0
8	21239.0
9	18539.0
10	16951.0
11	16084.0
12	14986.0
13	14848.0
14	15395.0
15	16501.0
16	17588.0
17	19094.0
18	21983.0
19	27779.0
20	38149.0
21	48190.0
22	60507.0
23	73307.0
24	86049.0
25	100234.0
26	113849.0
27	128724.0
28	142305.0
29	167177.0
30	192254.0
31	219868.0
32	275790.0
33	348866.0
34	439268.0
35	547259.0
36	683008.0
37	798993.0
38	1072551.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.46535295667905	16.95102628528735	12.431411468501409	25.152209289532195
2	15.166077548646415	19.034634318508818	40.444781839759365	25.354506293085404
3	16.818390535462985	23.45174560418215	30.131242386017576	29.59862147433729
4	11.306706706674687	14.871122557003192	36.418899391322995	37.403271344999126
5	14.02006728334805	37.153008756943905	34.129672634666264	14.697251325041783
6	35.171701023923525	34.51073081442285	16.759302037455935	13.558266124197688
7	31.108868240839715	30.213503628046062	20.173889771221905	18.50373835989232
8	26.99693063366372	34.72495846147673	19.679004296857077	18.599106608002476
9	26.780065137747854	15.077620457831525	18.968187634571223	39.174126769849394
10	13.107905411115182	28.995518051988295	34.32965076527934	23.56692577161719
11	36.64766918319774	22.024658839057597	21.80003993580216	19.527632041942507
12	22.96712386495784	24.387495295804	30.532078540009405	22.113302299228753
13	28.528583687584085	20.41851145623916	26.238854529549883	24.81405032662687
14	21.12784023555596	19.59144443810301	28.21562169722624	31.06509362911478
15	24.26819337592594	28.303412683720975	22.672345467525936	24.756048472827153
16	24.539193805176325	27.458883980862907	24.1341604310082	23.867761782952567
17	23.582488667406587	27.228341066901507	25.658315095092753	23.530855170599153
18	23.364579057148376	26.994524564964124	26.405961657247868	23.234934720639632
19	24.43612285857931	26.022938269374794	25.954822048170527	23.586116823875365
20	24.199870292310006	25.970112772651632	26.42410570487993	23.405911230158434
21	24.78158691051326	25.392045321025797	26.16917404834782	23.657193720113124
22	24.67957372094462	25.485317422445053	25.803877295858786	24.031231560751543
23	24.22047832669581	25.630527148559235	25.764247356648017	24.384747168096933
24	24.104306439772767	25.942165586848816	25.824091149756622	24.129436823621795
25	24.091498723189986	25.496812970311673	26.158819979778848	24.252868326719497
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	17191.0
1	17191.0
2	9520.5
3	1850.0
4	1850.0
5	1850.0
6	3030.5
7	4211.0
8	4211.0
9	4211.0
10	5887.0
11	7563.0
12	7563.0
13	7563.0
14	12284.0
15	17005.0
16	17005.0
17	17005.0
18	30018.0
19	43031.0
20	43031.0
21	43031.0
22	69086.0
23	95141.0
24	95141.0
25	95141.0
26	143772.5
27	192404.0
28	192404.0
29	192404.0
30	252237.5
31	312071.0
32	312071.0
33	312071.0
34	386695.0
35	461319.0
36	461319.0
37	461319.0
38	536385.5
39	611452.0
40	611452.0
41	611452.0
42	677889.5
43	744327.0
44	744327.0
45	744327.0
46	791609.0
47	838891.0
48	838891.0
49	838891.0
50	841822.5
51	844754.0
52	844754.0
53	844754.0
54	797587.0
55	750420.0
56	750420.0
57	750420.0
58	661045.5
59	571671.0
60	571671.0
61	571671.0
62	473753.0
63	375835.0
64	375835.0
65	375835.0
66	292517.5
67	209200.0
68	209200.0
69	209200.0
70	156306.5
71	103413.0
72	103413.0
73	103413.0
74	73304.5
75	43196.0
76	43196.0
77	43196.0
78	31109.5
79	19023.0
80	19023.0
81	19023.0
82	12566.0
83	6109.0
84	6109.0
85	6109.0
86	4135.5
87	2162.0
88	2162.0
89	2162.0
90	1400.0
91	638.0
92	638.0
93	638.0
94	371.0
95	104.0
96	104.0
97	104.0
98	86.0
99	68.0
100	68.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.3159069855822902
2	0.3174851655072358
3	0.32794260016142074
4	0.32896283768865825
5	0.3344784968202863
6	0.2821434999152725
7	0.28960398683319705
8	0.2871330990719186
9	0.28689398090147233
10	0.3965057502340569
11	0.28710121664919247
12	0.2868620984787461
13	0.2804537315107853
14	0.285188271285622
15	0.27901902248810745
16	0.28314779623114694
17	0.27769590194497124
18	0.27854078614721484
19	0.27999143638125573
20	0.4747292743927235
21	0.28034214303124366
22	0.2823188532402664
23	0.2781741382858639
24	0.2817290284198322
25	0.27457142451780625
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	6273049.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	62.98914566660443
#Duplication Level	Percentage of deduplicated	Percentage of total
1	86.99755434611697	54.79901623345897
2	6.504546056681681	8.194315981189199
3	2.4172164889207615	4.567752045850439
4	1.2171699227605886	3.0667397426310554
5	0.6846666374210939	2.156328327879076
6	0.425687068028661	1.608819884186825
7	0.3097285623853515	1.3656676273239219
8	0.21922792963087948	1.1047183994966059
9	0.16130125354939462	0.914420534042584
>10	0.9310945790544176	10.963593717141796
>50	0.07979179338141856	3.473220002711667
>100	0.04847588811742072	5.597691083869942
>500	0.0028366173232817794	1.2120805714007103
>1k	6.777546058484021E-4	0.7183028811686082
>5k	0.0	0.0
>10k+	2.5102022426494958E-5	0.2573329676484773
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
NNNNNNNNNNNNNNNNNNNNNNNNN	16275	0.25944321493423694	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	1.5941211363086754E-5	0.0
5	0.0	1.5941211363086754E-5	0.0	1.5941211363086754E-5	0.0
6	0.0	1.5941211363086754E-5	0.0	1.5941211363086754E-5	0.0
7	1.5941211363086754E-5	1.5941211363086754E-5	0.0	1.5941211363086754E-5	0.0
8	3.188242272617351E-5	1.5941211363086754E-5	0.0	1.5941211363086754E-5	0.0
9	3.188242272617351E-5	1.5941211363086754E-5	0.0	1.5941211363086754E-5	0.0
10	3.188242272617351E-5	1.5941211363086754E-5	0.0	3.188242272617351E-5	0.0
11	4.7823634089260265E-5	3.188242272617351E-5	0.0	3.188242272617351E-5	0.0
12	6.376484545234701E-5	3.188242272617351E-5	0.0	4.7823634089260265E-5	0.0
13	6.376484545234701E-5	3.188242272617351E-5	0.0	4.7823634089260265E-5	1.5941211363086754E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGACCGA	585	0.0	13.154372	6
AGGACCG	810	0.0	12.554708	5
GGTATCA	2845	0.0	11.845788	1
ACCGAGT	560	0.0	11.535898	8
TCGCGGT	285	0.0	11.341865	17
CTTCGCG	290	0.0	11.146316	15
CGAGTGG	565	0.0	10.929203	10
ACGGCCC	500	0.0	10.838153	15
TGGACCG	255	0.0	10.808506	5
CGCGGTT	320	0.0	10.695203	18
CGAGCGA	285	0.0	10.666667	10
TTCGCGG	310	0.0	10.427282	16
GGACCGT	240	0.0	10.292078	6
CATGGGT	3400	0.0	10.23081	4
GTTTACG	95	1.6573582E-4	9.992963	1
GTATCAA	6255	0.0	9.986572	1
TGCCGTA	300	0.0	9.824057	15
CGCGCCA	245	0.0	9.693878	10
CAGGACC	1825	0.0	9.686299	4
CGCCCTA	150	2.6251837E-7	9.506847	18
>>END_MODULE