FastQCFastQC Report
Thu 26 May 2016
SRR1364305_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364305_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6273049
Sequences flagged as poor quality0
Sequence length25
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT66540.10607282040997926No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGCGA602.5673386E-514.25198610
CGCGCCA2950.013.20523110
AGGACCG7850.013.19586855
TCGCGGT2450.013.179728517
GGACCGA6200.012.72042756
TTCGCGG3100.012.2546916
GCGCGAT701.0931006E-412.21287211
ACGTCGC1251.4169927E-912.15859111
CGAGTGG6150.012.0504610
GGTATCA38900.011.8461241
ACCGAGT6200.011.8007848
CTTCGCG2900.011.78985815
CGCGGTT3300.011.51161518
TGGTATC14550.010.9685892
ACGGCCC5200.010.95852215
CACGTCG1605.4387783E-1010.6889910
GTGGTAT17050.010.5879861
TGCACCG6200.010.5764165
TAGACAG11200.010.0974125
CAAGTCG2350.09.69945616
Produced by FastQC (version 0.11.5)