##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1364305_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 6273049 Sequences flagged as poor quality 0 Sequence length 25 %GC 49 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.932070034842706 31.0 30.0 34.0 26.0 34.0 2 29.973903599350173 31.0 30.0 34.0 26.0 34.0 3 30.0200966069291 31.0 30.0 34.0 26.0 34.0 4 33.43811613778244 35.0 33.0 37.0 28.0 37.0 5 32.99197089007276 35.0 33.0 37.0 26.0 37.0 6 32.91801450937176 35.0 33.0 37.0 26.0 37.0 7 32.65558694025824 35.0 33.0 37.0 25.0 37.0 8 32.56584015205365 35.0 33.0 37.0 25.0 37.0 9 33.37899321366691 37.0 33.0 39.0 19.0 39.0 10 33.503214624977424 37.0 33.0 39.0 22.0 39.0 11 33.478136708321586 37.0 33.0 39.0 22.0 39.0 12 33.34239426473474 37.0 33.0 39.0 19.0 39.0 13 33.47027577817422 37.0 33.0 39.0 22.0 39.0 14 34.44215372779648 38.0 33.0 41.0 22.0 41.0 15 34.471401387108564 38.0 33.0 41.0 22.0 41.0 16 34.40113539683812 38.0 33.0 41.0 22.0 41.0 17 34.30602582571888 38.0 33.0 41.0 19.0 41.0 18 34.234695919002064 38.0 33.0 41.0 19.0 41.0 19 34.10159063001102 39.0 33.0 41.0 18.0 41.0 20 33.94000349750177 39.0 33.0 41.0 18.0 41.0 21 33.86071494101194 39.0 33.0 41.0 17.0 41.0 22 33.83929792354563 39.0 33.0 41.0 17.0 41.0 23 33.53854289995184 38.0 33.0 41.0 10.0 41.0 24 33.44111165080968 38.0 33.0 41.0 2.0 41.0 25 33.29576701855828 38.0 33.0 41.0 2.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.21652086992183683 1101 2 0.4464254276380828 1101 3 -1.5551915287723048 1101 4 -0.5596548564646326 1101 5 0.4037633258139621 1101 6 0.7122178104183448 1101 7 0.8975777664209872 1101 8 0.9714925445517935 1101 9 1.7801352030940834 1101 10 1.4696373277404007 1101 11 1.3414357749801056 1101 12 1.4480928347728295 1101 13 1.166708835876065 1101 14 1.3749297397261913 1101 15 1.3509593772359167 1101 16 1.3530347208938736 1101 17 1.3248641304775504 1101 18 1.3445172930954854 1101 19 1.4006253275779912 1101 20 1.605209054096484 1101 21 1.4683737498451208 1101 22 1.552364927795871 1101 23 1.8248242016830858 1101 24 1.7653588519148684 1101 25 1.9137355530295608 1102 1 0.6674172982352715 1102 2 0.6961099130110284 1102 3 0.7912021946576182 1102 4 0.7639727676451642 1102 5 0.8770546572423115 1102 6 1.039143939925495 1102 7 1.0865211014284242 1102 8 1.1687455981484334 1102 9 1.794995012212567 1102 10 1.297846323873486 1102 11 1.2809447515033128 1102 12 1.5288581156832777 1102 13 1.216394261801696 1102 14 1.3579478461814674 1102 15 1.3929307249946703 1102 16 1.4060951848603551 1102 17 1.3876062744790048 1102 18 1.3968671029363406 1102 19 1.4557856291080142 1102 20 1.577950720520498 1102 21 1.7125699384851174 1102 22 1.6549634859071247 1102 23 2.0527390582152947 1102 24 1.994930605820585 1102 25 1.9168602064960183 1103 1 -3.0638606864551186 1103 2 -2.9031358156696285 1103 3 -2.9841101005883637 1103 4 -2.9512806372221405 1103 5 -6.305191640219821 1103 6 -10.836244860593816 1103 7 -11.774361407944543 1103 8 -12.024798225598182 1103 9 -13.013724248096906 1103 10 -10.85348263732265 1103 11 -13.036883340539802 1103 12 -12.41613832915111 1103 13 -12.695759497816887 1103 14 -13.4812915937162 1103 15 -13.293188778538102 1103 16 -7.527862240033816 1103 17 -5.446043036061958 1103 18 -4.822209427321052 1103 19 -4.432904149999043 1103 20 -9.44380702808619 1103 21 -6.094515453689375 1103 22 -11.413440984125607 1103 23 -13.207050589421534 1103 24 -14.78584419745237 1103 25 -15.89992812058642 1104 1 0.31275039522812165 1104 2 0.5236903253258589 1104 3 0.5692059885537226 1104 4 0.6794912445307517 1104 5 0.741919396916991 1104 6 0.7589781815060022 1104 7 0.6719312396139472 1104 8 0.8645770166830076 1104 9 1.3148958210115964 1104 10 1.044828087616537 1104 11 1.0208145023403716 1104 12 0.968351767822341 1104 13 0.8878158673424323 1104 14 1.001045154918529 1104 15 0.9788817179620395 1104 16 0.552315796623887 1104 17 0.6153410378166839 1104 18 0.767643765946417 1104 19 0.9192133974654837 1104 20 1.2871310812866383 1104 21 1.0774580343546205 1104 22 0.7735309303962907 1104 23 1.2306436378018333 1104 24 1.4861188004442951 1104 25 1.469147670332866 1105 1 0.26128644531112144 1105 2 0.3184403625963874 1105 3 -0.020378135530105368 1105 4 0.3029985727726867 1105 5 0.3835847854311112 1105 6 0.4944425859770831 1105 7 0.5874659137989795 1105 8 0.44830188097858326 1105 9 0.5700306405006188 1105 10 0.1353250790282985 1105 11 0.737287174477089 1105 12 0.8802303184864329 1105 13 0.7119864667281277 1105 14 0.49003571780385613 1105 15 0.35476327730913226 1105 16 0.6626280521528614 1105 17 0.5876338670133734 1105 18 0.625214844934689 1105 19 0.6922957532341201 1105 20 0.9243428759625516 1105 21 0.9149843293204185 1105 22 0.6315693783341914 1105 23 1.0579541857414583 1105 24 1.1420666934342947 1105 25 1.1841387560480428 1106 1 -0.12169414194266537 1106 2 0.04610225962024472 1106 3 -0.07346908733781277 1106 4 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17 -0.2115499654086861 2213 18 -0.396803596296607 2213 19 -0.2677908645185596 2213 20 0.0758885482575522 2213 21 -0.1525341738093431 2213 22 0.020592871181783323 2213 23 -0.3625693700070016 2213 24 -0.17258424684597173 2213 25 0.10223638320678674 2214 1 -1.0823168596495627 2214 2 -0.8901439859757119 2214 3 -0.8779665118048179 2214 4 -0.7525566491499802 2214 5 -1.0984037873655623 2214 6 -0.9264541327545999 2214 7 -0.8786167600967723 2214 8 -0.8565943289906635 2214 9 -10.96861474460012 2214 10 -4.092439084643566 2214 11 -1.9024396300708446 2214 12 -1.3663787848696565 2214 13 -1.1243278087966573 2214 14 -1.1407473523779288 2214 15 -1.1784287929798865 2214 16 -1.2354172725946952 2214 17 -1.2519315401337394 2214 18 -1.5775400238469999 2214 19 -1.6910305156169017 2214 20 -1.3616880438250618 2214 21 -1.9779006080693833 2214 22 -1.695120189292556 2214 23 -2.1503603790796113 2214 24 -2.4017921000505567 2214 25 -10.02078820910921 2215 1 -0.5977126190941604 2215 2 -0.3290417027915318 2215 3 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-1.6001751188557307 2216 15 -1.2632515950859826 2216 16 -2.2557257722007975 2216 17 -2.5514692402507926 2216 18 -2.016464917362697 2216 19 -3.4266752830872242 2216 20 -2.2476266768202855 2216 21 -2.443945160123185 2216 22 -2.2679351491690056 2216 23 -1.8066067817571856 2216 24 -2.1392684598673632 2216 25 -2.123947977848001 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 179810.0 3 2.0 4 2718.0 5 28074.0 6 34652.0 7 27363.0 8 22186.0 9 19228.0 10 17139.0 11 15608.0 12 14165.0 13 13793.0 14 13620.0 15 14390.0 16 15089.0 17 16368.0 18 18088.0 19 21292.0 20 26769.0 21 35031.0 22 44630.0 23 54243.0 24 64405.0 25 74650.0 26 84378.0 27 94637.0 28 108341.0 29 126606.0 30 155228.0 31 192435.0 32 237256.0 33 292837.0 34 364516.0 35 450927.0 36 583422.0 37 830084.0 38 1979069.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.36797370112972 17.903970726306014 12.306244195777078 26.42181137678719 2 17.138599414662576 19.34208762270716 37.42119951927024 26.09811344336002 3 19.703910656382018 22.61195889265155 28.288394189801785 29.395736261164647 4 13.083851269821862 15.279008176400716 34.74271255509294 36.894427998684485 5 14.366828249911379 37.05819946863941 33.96920557907892 14.605766702370296 6 35.509946391916934 34.874620857330754 16.190374459435223 13.425058291317086 7 31.189355373208006 30.259906910059403 19.816482838056924 18.734254878675667 8 27.49440592100952 35.58314197408124 18.28522762914537 18.63722447576388 9 26.47519164219794 14.862035637132667 18.228043013070945 40.43472970759844 10 14.986746241313135 29.267364241866183 32.36408079896994 23.381808717850745 11 37.25698513253269 21.601332184268056 21.200860395585497 19.94082228761376 12 23.43552214306986 24.506167486000958 29.535173131532012 22.523137239397172 13 29.60396958218062 20.3205948379235 24.876651986592492 25.198783593303386 14 22.397711096929726 19.658284256904405 26.255986458141862 31.68801818802401 15 24.739632488355838 28.44267961085802 21.94353538651471 24.87415251427143 16 24.904233067411504 27.34548474935114 23.79227168665863 23.958010496578726 17 23.883508116542263 27.104106426258245 25.47443680980773 23.537948647391755 18 23.566889339098296 26.914198225391228 26.139439103987268 23.37947333152321 19 24.713258522844132 25.97417485195374 25.475691586737682 23.836875038464445 20 24.36547745855214 25.831974550381947 26.01092415256825 23.791623838497667 21 24.849402968703366 25.29961571132306 25.722087734648223 24.12889358532535 22 24.889193502795813 25.317241938957864 25.584236134659967 24.209328423586363 23 24.10698784531937 25.358900341056756 25.933211033512137 24.600900780111733 24 24.334050670385192 25.759090489526344 25.589037130043874 24.317821710044598 25 24.2137178976731 25.36843222316817 25.884319518837117 24.533530360321613 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 179.0 1 179.0 2 397.5 3 616.0 4 616.0 5 616.0 6 1651.5 7 2687.0 8 2687.0 9 2687.0 10 3835.0 11 4983.0 12 4983.0 13 4983.0 14 8133.0 15 11283.0 16 11283.0 17 11283.0 18 22641.5 19 34000.0 20 34000.0 21 34000.0 22 57316.0 23 80632.0 24 80632.0 25 80632.0 26 127787.0 27 174942.0 28 174942.0 29 174942.0 30 229400.5 31 283859.0 32 283859.0 33 283859.0 34 357952.5 35 432046.0 36 432046.0 37 432046.0 38 507525.0 39 583004.0 40 583004.0 41 583004.0 42 651040.5 43 719077.0 44 719077.0 45 719077.0 46 776655.0 47 834233.0 48 834233.0 49 834233.0 50 851248.5 51 868264.0 52 868264.0 53 868264.0 54 829391.5 55 790519.0 56 790519.0 57 790519.0 58 700494.5 59 610470.0 60 610470.0 61 610470.0 62 512575.0 63 414680.0 64 414680.0 65 414680.0 66 323417.5 67 232155.0 68 232155.0 69 232155.0 70 173920.0 71 115685.0 72 115685.0 73 115685.0 74 81557.0 75 47429.0 76 47429.0 77 47429.0 78 34812.5 79 22196.0 80 22196.0 81 22196.0 82 14643.5 83 7091.0 84 7091.0 85 7091.0 86 4673.5 87 2256.0 88 2256.0 89 2256.0 90 1472.5 91 689.0 92 689.0 93 689.0 94 377.5 95 66.0 96 66.0 97 66.0 98 37.0 99 8.0 100 8.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.02794494351949108 2 0.001370944177225461 3 1.5941211363086754E-4 4 0.003825890727140821 5 0.0315317160761856 6 0.010744376458720473 7 0.020165632374304743 8 0.026876882358164267 9 0.03376348566701775 10 0.037860376987331044 11 0.038976261782747115 12 0.0076039578201923825 13 0.0023433580703737526 14 0.005579423977080364 15 0.006009836683883707 16 0.008432900811072893 17 0.009756021354209093 18 0.0144427374949566 19 0.01630785922443775 20 0.014219560535873386 21 0.01085596493826208 22 0.01001108073601848 23 0.007747428722460163 24 0.004973657945283067 25 0.10463811138730146 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 6273049.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 40.247345578094965 #Duplication Level Percentage of deduplicated Percentage of total 1 71.04742651976616 28.594703275753375 2 13.100115982073326 10.5448979008726 3 5.528910079502679 6.675718639198704 4 2.8673280005136834 4.61609363689689 5 1.7506783022031052 3.523007731242047 6 1.1541591493636045 2.787110528193427 7 0.7839789660755014 2.2087150661518806 8 0.5485861204647531 1.7663308135753077 9 0.42182700744772866 1.5279675608630137 >10 2.448083070495014 18.26013083678483 >50 0.21052957525535934 5.89605584988035 >100 0.12872850285457402 9.767051357172328 >500 0.006857791589094312 1.8565665190320633 >1k 0.0026713210068854284 1.704282799912845 >5k 1.1961138832894302E-4 0.27136748447046405 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 6654 0.10607282040997926 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 1.5941211363086754E-5 0.0 0.0 0.0 0.0 8 1.5941211363086754E-5 0.0 0.0 0.0 0.0 9 1.5941211363086754E-5 0.0 0.0 0.0 0.0 10 4.7823634089260265E-5 0.0 0.0 0.0 0.0 11 7.970605681543377E-5 0.0 0.0 1.5941211363086754E-5 0.0 12 7.970605681543377E-5 0.0 0.0 3.188242272617351E-5 0.0 13 7.970605681543377E-5 0.0 0.0 3.188242272617351E-5 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGCGCGA 60 2.5673386E-5 14.251986 10 CGCGCCA 295 0.0 13.205231 10 AGGACCG 785 0.0 13.1958685 5 TCGCGGT 245 0.0 13.1797285 17 GGACCGA 620 0.0 12.7204275 6 TTCGCGG 310 0.0 12.25469 16 GCGCGAT 70 1.0931006E-4 12.212872 11 ACGTCGC 125 1.4169927E-9 12.158591 11 CGAGTGG 615 0.0 12.05046 10 GGTATCA 3890 0.0 11.846124 1 ACCGAGT 620 0.0 11.800784 8 CTTCGCG 290 0.0 11.789858 15 CGCGGTT 330 0.0 11.511615 18 TGGTATC 1455 0.0 10.968589 2 ACGGCCC 520 0.0 10.958522 15 CACGTCG 160 5.4387783E-10 10.68899 10 GTGGTAT 1705 0.0 10.587986 1 TGCACCG 620 0.0 10.576416 5 TAGACAG 1120 0.0 10.097412 5 CAAGTCG 235 0.0 9.699456 16 >>END_MODULE