FastQCFastQC Report
Thu 26 May 2016
SRR1364304_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364304_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1122324
Sequences flagged as poor quality0
Sequence length25
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAA111900.9970382884086948No Hit
GTATCAACGCAGAGTACTTTTTTTT61380.5469008949287372No Hit
GAAAAAAAAAAAAAAAAAAAAAAAA57920.516072007726824No Hit
GTACTTTTTTTTTTTTTTTTTTTTT50650.45129570427078103No Hit
TATCAACGCAGAGTACTTTTTTTTT35930.32013928241755496No Hit
GAACAAAAAAAAAAAAAAAAAAAAA33070.29465644501944177No Hit
NNNNNNNNNNNNNNNNNNNNNNNNN32900.29314173090836515No Hit
GGTATCAACGCAGAGTACTTTTTTT27200.24235425777226538No Hit
GAGTACTTTTTTTTTTTTTTTTTTT21640.19281419625705232No Hit
AAACAAAAAAAAAAAAAAAAAAAAA21310.18987386886496235No Hit
ACGCAGAGTACTTTTTTTTTTTTTT14010.12483026291872935No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATAACG1500.015.8221082
CGCATAT551.1286231E-515.5441738
ATAACGC1650.015.5344343
TAACGCA1650.014.9751664
GACGTCT704.3853834E-714.94272715
GAACAAA8250.014.3830931
GAGACCG602.5430654E-514.26415217
GAGTGCG400.005280759714.2488257
CGAGTGG400.005282328514.24818810
CGTCTCG551.9429435E-413.83190517
GTATAAC2250.013.500931
TGACCGA1550.013.49726219
GGCGTAG500.001500524513.29830810
ATCGTCT655.4015516E-513.16631915
GCGTAGG655.5162047E-513.13688111
TTGACCG1600.013.07547418
AGGACCG957.348535E-813.0041685
TTTGCGC1700.012.865705517
GGACGTC604.0563112E-412.67924614
CGGGGAT604.094818E-412.66505410