##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1364304_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1122324
Sequences flagged as poor quality	0
Sequence length	25
%GC	43
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	24.171223283116106	30.0	22.0	31.0	2.0	34.0
2	24.109553034596072	30.0	16.0	31.0	2.0	34.0
3	23.72765974887822	29.0	16.0	31.0	2.0	34.0
4	27.053186067481406	33.0	20.0	35.0	2.0	37.0
5	27.778525630744777	35.0	26.0	35.0	2.0	37.0
6	27.761950203328094	35.0	26.0	37.0	2.0	37.0
7	27.34297760717939	35.0	24.0	37.0	2.0	37.0
8	26.848600760564686	35.0	19.0	37.0	2.0	37.0
9	27.718403063642942	35.0	18.0	39.0	2.0	39.0
10	27.10914406178608	34.0	17.0	39.0	2.0	39.0
11	27.670325146749068	34.0	19.0	39.0	2.0	39.0
12	27.25026552047359	34.0	17.0	39.0	2.0	39.0
13	26.855621905973678	34.0	17.0	39.0	2.0	39.0
14	27.073970618110277	33.0	16.0	39.0	2.0	41.0
15	27.708771263913096	34.0	17.0	39.0	2.0	41.0
16	28.016758975126614	34.0	18.0	40.0	2.0	41.0
17	28.153003945384754	35.0	19.0	40.0	2.0	41.0
18	27.964514703418978	35.0	17.0	40.0	2.0	41.0
19	27.91574358206721	35.0	18.0	40.0	2.0	41.0
20	27.7630817838699	35.0	18.0	40.0	2.0	41.0
21	27.652598536607968	35.0	17.0	40.0	2.0	41.0
22	27.680198409728384	35.0	17.0	40.0	2.0	41.0
23	27.63438543593472	35.0	16.0	40.0	2.0	41.0
24	27.50105317181135	35.0	10.0	40.0	2.0	41.0
25	27.288728566795328	35.0	2.0	40.0	2.0	41.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1101	1	0.3891155995990836
1101	2	0.4106159970514014
1101	3	1.1201565653630645
1101	4	0.62012683171967
1101	5	0.1017494256588023
1101	6	0.08269237177444566
1101	7	0.31119497052089784
1101	8	0.7428737318351821
1101	9	0.4880744753642112
1101	10	0.9744688184901129
1101	11	0.35647058819372646
1101	12	0.6158560777395898
1101	13	0.4624270808626534
1101	14	0.8179898720348255
1101	15	0.14460962430278101
1101	16	-0.33466714775139295
1101	17	-0.7578058189540222
1101	18	-6.120303011598331
1101	19	-7.453855821098294
1101	20	-8.666814942510857
1101	21	-10.194256856989302
1101	22	-10.56746312136211
1101	23	-11.62769422944552
1101	24	-11.317627686567667
1101	25	-11.787677548244337
1102	1	1.6705184498717962
1102	2	1.8493722772304686
1102	3	2.5336259610974317
1102	4	1.8629767134470363
1102	5	1.4702126633965698
1102	6	1.495487320262317
1102	7	1.5830363092498096
1102	8	1.9717029305136542
1102	9	2.0390959208317305
1102	10	-1.3545969254545227
1102	11	0.7582221961150601
1102	12	1.6857385870743293
1102	13	2.0169400126555104
1102	14	2.6657309624889507
1102	15	2.262010363262373
1102	16	1.9285920941308028
1102	17	1.765156666139717
1102	18	1.885024144189206
1102	19	1.4485330905979907
1102	20	1.8121012500878173
1102	21	1.8279647135930688
1102	22	1.6458029627385287
1102	23	1.584527927612715
1102	24	1.7776023097179845
1102	25	1.889559368456542
1103	1	-0.984439664291461
1103	2	-1.0449780052554054
1103	3	-2.4961422056666436
1103	4	-1.0364972783706143
1103	5	-3.360685446870331
1103	6	-7.662743733702229
1103	7	-8.984000511322954
1103	8	-4.010763463408029
1103	9	-7.537662310222235
1103	10	-5.878734856489007
1103	11	-7.446297577896239
1103	12	-3.5492117343751133
1103	13	-0.8153997496667174
1103	14	-0.43608084347247456
1103	15	-1.4176701354713117
1103	16	-1.1533482916085305
1103	17	-1.0663648612321879
1103	18	-0.8378864344197936
1103	19	-6.146362681854768
1103	20	-2.447688346639243
1103	21	-5.870087107953783
1103	22	-2.7336047910117145
1103	23	-1.7060818522431198
1103	24	-6.543340608653338
1103	25	-4.425636423478
1104	1	1.7997934453980378
1104	2	1.6744790083192562
1104	3	2.5501871748919456
1104	4	2.0087125827621684
1104	5	1.8057796802945951
1104	6	1.9050905618196943
1104	7	1.9288642101624554
1104	8	2.3298669001432266
1104	9	2.3982754335755487
1104	10	2.6257198166269227
1104	11	2.142503327081137
1104	12	2.3014318917756107
1104	13	2.5693053525333127
1104	14	3.0952500973919364
1104	15	2.13231036070594
1104	16	1.2711797158809368
1104	17	1.1846571390798708
1104	18	1.0657607804343172
1104	19	1.4018468672168503
1104	20	-8.069197162367125
1104	21	-2.795512291289718
1104	22	-0.6435322367910885
1104	23	0.4969841486930662
1104	24	1.0395389357373617
1104	25	1.3194447124246373
1105	1	0.70949888207846
1105	2	0.6398423887191029
1105	3	1.4410216573612864
1105	4	0.469895817086293
1105	5	0.5416000847555118
1105	6	0.5557003466909478
1105	7	0.7477651817285498
1105	8	0.9468742878206449
1105	9	0.953143619373801
1105	10	1.39013076863586
1105	11	0.9384069412231106
1105	12	1.1341782044909117
1105	13	1.275226374119594
1105	14	1.5971193003721744
1105	15	1.1265461109825203
1105	16	0.622849414868476
1105	17	-0.24243870148471558
1105	18	0.44234291787972424
1105	19	0.38311747957089537
1105	20	0.3561435149502401
1105	21	0.3993658828161841
1105	22	0.38973000740599417
1105	23	0.49060972085793253
1105	24	0.3403410151929087
1105	25	0.33847034121613007
1106	1	1.0027860410376945
1106	2	1.1811583583809586
1106	3	1.7020356940198766
1106	4	1.5103091479616744
1106	5	0.6761288164525538
1106	6	0.46019905626642554
1106	7	0.9137855388337854
1106	8	0.8524810506985538
1106	9	1.0283839280222793
1106	10	-2.2483469254545234
1106	11	-0.37982030516896614
1106	12	0.2713799584148546
1106	13	1.182797742496291
1106	14	0.12877025370620743
1106	15	0.9787444413311981
1106	16	0.6129526488303405
1106	17	-5.623261412956328
1106	18	-2.065139569216033
1106	19	-0.7762999859300024
1106	20	-0.0746475942881446
1106	21	0.31970521179543354
1106	22	0.38857389237386286
1106	23	-0.018248780142805288
1106	24	0.2040339994971312
1106	25	0.4044380279326596
1107	1	-0.049821177756061985
1107	2	0.25279723718612246
1107	3	0.735212909702085
1107	4	0.04387725199113035
1107	5	-0.3595642162705701
1107	6	-0.07923172473238083
1107	7	0.030327635499240557
1107	8	0.0244628481367144
1107	9	-0.12875934584589643
1107	10	-2.8399363435948537
1107	11	-1.1730517136634937
1107	12	-0.6952431681733309
1107	13	0.22540911002209185
1107	14	0.42660578241024893
1107	15	-0.0024686090240386704
1107	16	-5.916583730363769
1107	17	-2.9819893079934054
1107	18	-1.3744680580403852
1107	19	-0.5053177736186392
1107	20	0.12091326242502021
1107	21	0.15248700145471616
1107	22	-0.14092229597856942
1107	23	-0.4588878793493585
1107	24	-0.19565290088143783
1107	25	-0.07774414029842802
1108	1	-0.11504437957050584
1108	2	-2.39947535975276
1108	3	0.29122019778771246
1108	4	-0.2456034836454002
1108	5	-0.4846693103177948
1108	6	0.0640701080541426
1108	7	0.006974371605952712
1108	8	0.42882785204535523
1108	9	-0.1499025745697793
1108	10	-2.8634740565538763
1108	11	-1.2093781187545574
1108	12	-4.935467283849054
1108	13	-1.2549140313265958
1108	14	-5.080918565704831
1108	15	-2.228690022353266
1108	16	-6.721334668790309
1108	17	-2.9656776387058876
1108	18	-1.6551217321148606
1108	19	-0.23704767445546793
1108	20	-0.046909899883786466
1108	21	0.24883279717301576
1108	22	0.23469783016446755
1108	23	0.322140897052428
1108	24	0.4816507757250186
1108	25	0.05668837532189386
1109	1	-7.601551175989236
1109	2	-5.698716060342534
1109	3	-5.087094115883541
1109	4	-4.837683531786908
1109	5	-2.5310640012693533
1109	6	-1.6370609595749563
1109	7	-1.225511627602927
1109	8	-0.7306874811630628
1109	9	-5.645913749426747
1109	10	-1.811820084532659
1109	11	-1.4665336655859527
1109	12	-5.8763501887566925
1109	13	-7.5924577636614075
1109	14	-7.714400705463905
1109	15	-3.9108995747145876
1109	16	-2.5220133688506863
1109	17	-2.033913464670775
1109	18	-1.550018395420743
1109	19	-1.1473434730780419
1109	20	-0.8803607119742836
1109	21	-1.001780620388125
1109	22	-1.0782269150255956
1109	23	-1.3103577918449858
1109	24	-1.0039984753355107
1109	25	-0.9750655874794738
1110	1	-2.6764446313370236
1110	2	-2.430573573122441
1110	3	-1.6785091939176695
1110	4	-2.4977256669464083
1110	5	-5.231695094401136
1110	6	-3.904027523940073
1110	7	-8.210268540743147
1110	8	-8.761589712333425
1110	9	-10.02074569927563
1110	10	-8.271494432379733
1110	11	-10.473998408921169
1110	12	-6.161380996004688
1110	13	-4.692579996108595
1110	14	-4.108962685100138
1110	15	-9.157702612307684
1110	16	-10.164848912489227
1110	17	-10.982402687194554
1110	18	-8.336023792333634
1110	19	-11.092756797816175
1110	20	-11.891016267166513
1110	21	-12.962684299662639
1110	22	-13.423431899618077
1110	23	-14.136743806584507
1110	24	-14.663848775622485
1110	25	-15.1163423170686
1111	1	-1.081320355121516
1111	2	-0.5100546662434802
1111	3	-2.8864416045191774
1111	4	-3.1163108639117425
1111	5	-1.4018650274377968
1111	6	-0.9259758314523303
1111	7	-0.32985024403751595
1111	8	-0.2596698449702295
1111	9	-4.35691077798154
1111	10	-4.4503379816725825
1111	11	-1.6632197811898237
1111	12	-5.872492002247991
1111	13	-1.4054798373911694
1111	14	-0.3358282440481659
1111	15	-0.38273308951388074
1111	16	-0.4719603654693856
1111	17	-0.5746810608836412
1111	18	-0.2847994344785363
1111	19	-0.017642714751435307
1111	20	0.08404323220506527
1111	21	0.17101806348093973
1111	22	-0.18608281094553192
1111	23	-0.0509055221798711
1111	24	0.0033080431706657976
1111	25	0.15484488612273495
1112	1	0.1114337747707026
1112	2	0.6042545132210009
1112	3	-1.800915685896399
1112	4	-0.06133505845726006
1112	5	0.012312533957334892
1112	6	0.21690967438622266
1112	7	0.5295004982632143
1112	8	-3.2342172282822723
1112	9	-0.6160140382808876
1112	10	-2.511163156267486
1112	11	-0.5696631265034036
1112	12	0.8525957189192717
1112	13	1.1799306692625322
1112	14	1.6808782437832157
1112	15	1.2724123957474482
1112	16	1.1368479405766863
1112	17	0.854538473342707
1112	18	0.9484027973145395
1112	19	0.9849305686206193
1112	20	1.1394203161981835
1112	21	1.0808411232120427
1112	22	0.9951517516947597
1112	23	0.8740243993496755
1112	24	0.9143685129086769
1112	25	0.9294712983859128
1113	1	-2.003432181455697
1113	2	-1.4700339336670396
1113	3	-3.1545854672573235
1113	4	-2.4641760564312456
1113	5	-2.1475403415706324
1113	6	-2.0434426459910497
1113	7	-2.2697676948452887
1113	8	-8.13070483500411
1113	9	-9.459933389447723
1113	10	-4.079876567512688
1113	11	-8.855693551050603
1113	12	-7.874614804652058
1113	13	-3.9843112670623846
1113	14	-2.6533453655738413
1113	15	-7.576475967454648
1113	16	-4.247724593390526
1113	17	-3.326057833537984
1113	18	-3.015916211477574
1113	19	-2.5526575210123674
1113	20	-7.684979097237097
1113	21	-4.435588920805749
1113	22	-3.7004488857428726
1113	23	-3.690082469384212
1113	24	-8.665873972843727
1113	25	-6.424386712762889
1114	1	-4.344798067766888
1114	2	-3.9386787926569795
1114	3	-1.0766782593495883
1114	4	-1.1610075766522918
1114	5	-1.176092141723128
1114	6	-1.3007523446948177
1114	7	-0.9274782662587597
1114	8	-0.459081992557401
1114	9	-0.8428599363810356
1114	10	-3.3128646419899574
1114	11	-1.676893753374305
1114	12	-4.517963916214455
1114	13	-1.735775219974311
1114	14	-5.570804089273647
1114	15	-2.4212076243196883
1114	16	-1.6798329120654572
1114	17	-1.4827102318539644
1114	18	-1.125757347107431
1114	19	-0.8595855979557712
1114	20	-0.7697084389552913
1114	21	-0.6381604953320874
1114	22	-0.8012282346329869
1114	23	-1.0605172891928092
1114	24	-0.7113829654277062
1114	25	-0.6560813247618924
1115	1	0.8772380075109361
1115	2	1.104503718694403
1115	3	1.5288102935338372
1115	4	0.7007715667485641
1115	5	0.36333961097717804
1115	6	-0.09841542322571328
1115	7	-1.930792090983207
1115	8	0.08797800055720728
1115	9	0.4109314779900437
1115	10	1.2734445366294906
1115	11	0.21927798129634013
1115	12	1.0514278803510742
1115	13	1.0781344979394305
1115	14	1.7765985778643199
1115	15	1.255874270721666
1115	16	0.8738240469357805
1115	17	0.5197554992095199
1115	18	0.8072365032930193
1115	19	1.2049701623614233
1115	20	1.2833348488006813
1115	21	1.2465033693116752
1115	22	0.8654734112946585
1115	23	0.752552005950534
1115	24	0.7938191257184286
1115	25	1.0531302488323249
1116	1	1.063084530014656
1116	2	1.0443952917473212
1116	3	1.78860833459456
1116	4	1.4221140154375256
1116	5	0.4336018781320199
1116	6	-0.3403722219104388
1116	7	-3.9740597051592808
1116	8	-0.3624124345627777
1116	9	-0.09793666895567554
1116	10	0.5527600227743577
1116	11	-1.3547444443812537
1116	12	0.40768011942550686
1116	13	-5.519524526515351
1116	14	-0.4765957358900259
1116	15	-0.13632089183699492
1116	16	0.4190091264127034
1116	17	0.7414524835014369
1116	18	0.7318213547373986
1116	19	1.027873905573589
1116	20	1.218589958349952
1116	21	1.2118822615848792
1116	22	1.144004905502424
1116	23	1.0279968849550407
1116	24	1.2389764072256426
1116	25	1.3297708864909943
1201	1	2.3057243342994838
1201	2	2.3925929368558307
1201	3	2.892377892967353
1201	4	2.550061882457598
1201	5	2.133737035418605
1201	6	1.9056542466492559
1201	7	2.2673126516188447
1201	8	2.470531303510441
1201	9	2.8757119901417703
1201	10	3.004540418472832
1201	11	2.469139425508981
1201	12	2.6869775489627834
1201	13	2.6941974948438308
1201	14	3.425980293949735
1201	15	2.906029422241321
1201	16	2.393847293345871
1201	17	2.0351381330141614
1201	18	2.267640200673558
1201	19	2.2083713586164855
1201	20	2.4843246479985126
1201	21	2.465178460170211
1201	22	1.3579604365901474
1201	23	0.9022863813472384
1201	24	1.365806520392809
1201	25	1.500881248446536
1202	1	1.5444032820643834
1202	2	1.5652582152170353
1202	3	2.319272246837137
1202	4	1.94022976867954
1202	5	1.2423555523151144
1202	6	1.2740508049114823
1202	7	1.5843327573632884
1202	8	1.8648775125044672
1202	9	2.1160826957899452
1202	10	-1.5024034496390009
1202	11	0.7477406673231073
1202	12	1.6350169611758716
1202	13	2.1219300668648344
1202	14	2.5833016475092556
1202	15	2.3003896760177724
1202	16	1.937714894458729
1202	17	1.5988420741077896
1202	18	1.936672349180533
1202	19	1.927815976847377
1202	20	1.832091336072832
1202	21	1.9445166700222458
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2211	14	-4.794843039048551
2211	15	-1.3915027116238399
2211	16	-0.29035581376538033
2211	17	-0.025745214252225423
2211	18	0.5009867315889949
2211	19	0.7272539115763799
2211	20	0.9560317695445661
2211	21	0.9616586282493884
2211	22	0.811207899835086
2211	23	0.6573537625628703
2211	24	0.9842642174433323
2211	25	1.0118440263618744
2212	1	1.1356768140807922
2212	2	1.3227397927135947
2212	3	1.9199694071533493
2212	4	1.4162202431465545
2212	5	1.0574571654151903
2212	6	1.02941793532964
2212	7	1.3051644198405405
2212	8	1.6668863274100865
2212	9	1.3833056397952745
2212	10	2.233003288334892
2212	11	1.6071497764597211
2212	12	1.8668925063960025
2212	13	1.9400362148737251
2212	14	-3.2809213307649046
2212	15	-0.1962285313134764
2212	16	0.2494936332334312
2212	17	0.8909288596251805
2212	18	1.0475629841098524
2212	19	1.6333715557832278
2212	20	1.8053388009307447
2212	21	1.74727038112637
2212	22	1.6987179572881068
2212	23	1.5229345321355119
2212	24	1.304994229806642
2212	25	1.3941648998047746
2213	1	1.013029353642569
2213	2	1.1607766092939968
2213	3	1.8560536833595265
2213	4	1.265910149837108
2213	5	0.9125745029593162
2213	6	0.6038330756129859
2213	7	0.7674802629478918
2213	8	-4.487808654167697
2213	9	-1.2318365586029287
2213	10	0.709355300386953
2213	11	0.5461019877932287
2213	12	1.2836498478630816
2213	13	-6.928743566357721
2213	14	-1.7343960181327027
2213	15	-0.15159128564540936
2213	16	0.29123218688186725
2213	17	0.6786386956213057
2213	18	0.8939679966708489
2213	19	1.199067697418137
2213	20	1.4895612695949865
2213	21	1.2899975077188337
2213	22	0.9903234728693739
2213	23	0.9786573742348494
2213	24	1.1816509672199622
2213	25	1.309568938010635
2214	1	-0.4105446341350891
2214	2	-0.05933207224105175
2214	3	-5.871239824321432
2214	4	-1.9717848180138695
2214	5	-1.017654410554357
2214	6	-0.578892517364622
2214	7	0.00461156222559822
2214	8	-5.311381256584806
2214	9	-1.1273743122754354
2214	10	0.42924145838385996
2214	11	0.17730688781349002
2214	12	0.5072219307699832
2214	13	-5.539039046445762
2214	14	-0.9871853550125564
2214	15	-0.13661640396385977
2214	16	0.03541410550232271
2214	17	0.2590086561558458
2214	18	0.31107321653622577
2214	19	0.798101927945595
2214	20	1.0003125333035605
2214	21	0.9781814212584834
2214	22	0.7073272150745566
2214	23	0.737981283653049
2214	24	0.9648593583488072
2214	25	0.9751151943335081
2215	1	0.646108181961818
2215	2	0.7284508811834023
2215	3	-1.8380195580599086
2215	4	0.035775846368011344
2215	5	0.25620236060410306
2215	6	-0.15827656265710033
2215	7	0.2680848021393878
2215	8	0.6077857515104235
2215	9	0.7278245163754917
2215	10	1.4467261175443724
2215	11	0.7378259061169921
2215	12	0.5782376060043113
2215	13	1.0357921295866
2215	14	1.2111685197180364
2215	15	0.9449272868759522
2215	16	0.6599657790298004
2215	17	0.6852832397448871
2215	18	0.7384242525740845
2215	19	0.8994191681243215
2215	20	1.1235582792209264
2215	21	1.1238641523587276
2215	22	0.9787588177021185
2215	23	0.8850256259913039
2215	24	0.9516447542330262
2215	25	1.0462184980138431
2216	1	0.5418024263895838
2216	2	0.37847476347423736
2216	3	1.4396584607753802
2216	4	0.24148018241039892
2216	5	-0.2079466696791208
2216	6	-0.26370221211774236
2216	7	-0.6391429726655744
2216	8	-5.370544810519487
2216	9	-1.6086009451393046
2216	10	-0.24504209374985564
2216	11	-0.796460444345751
2216	12	0.595810542759935
2216	13	1.3530153912970029
2216	14	2.0473629395220208
2216	15	1.619852796873591
2216	16	0.9405264322673155
2216	17	0.9044801722771147
2216	18	1.0068887349987534
2216	19	1.0904900644105382
2216	20	1.1497132782811974
2216	21	1.021267866025024
2216	22	0.9441779788411431
2216	23	0.5063446706517851
2216	24	0.6317052560364687
2216	25	0.7829674179237749
>>END_MODULE
>>Per sequence quality scores	fail
#Quality	Count
2	158526.0
3	5.0
4	940.0
5	6566.0
6	9876.0
7	8660.0
8	7305.0
9	6116.0
10	5680.0
11	5206.0
12	5197.0
13	5087.0
14	4992.0
15	4924.0
16	4833.0
17	4969.0
18	5562.0
19	7585.0
20	10532.0
21	13474.0
22	16110.0
23	18116.0
24	20075.0
25	21706.0
26	23359.0
27	24743.0
28	26274.0
29	29665.0
30	33291.0
31	37041.0
32	44747.0
33	55216.0
34	67140.0
35	82717.0
36	100485.0
37	114225.0
38	131379.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	48.21797910953055	18.229219294488374	11.839393226737869	21.713408369243208
2	14.33209987605811	23.78709537802276	39.64351952040033	22.237285225518793
3	15.707693133239129	32.10188348507342	28.795141932137298	23.395281449550147
4	10.228285999204017	19.257768655281435	36.259508190123555	34.254437155390995
5	11.540845486134403	42.17162840826256	31.90433386550751	14.383192240095525
6	27.99275615579271	39.51211314841746	18.467869743095598	14.02726095269423
7	26.689098587218098	35.897926418056144	20.948487633241676	16.464487361484082
8	25.38773999653157	36.33474633088458	20.512004519685124	17.76550915289873
9	27.03237606220266	19.61568511065859	18.828318729629757	34.523620097508996
10	13.481363216146242	33.6658650941885	31.08770730284136	21.7650643868239
11	31.8105958145576	28.091503700320107	22.025374389798042	18.07252609532425
12	22.02378345134476	31.119527799355843	28.29002473457486	18.566664014724534
13	26.373274719302497	26.28344079139668	25.778314894286474	21.564969595014354
14	20.451061281224998	25.5326071896272	28.034540245553973	25.98179128359383
15	22.197612037419397	32.7729004324361	23.437625695178134	21.591861834966362
16	21.16812070938215	32.70675414759726	24.590067219679636	21.53505792334096
17	20.668964956297042	32.30488298654902	25.619970629324374	21.406181427829566
18	19.96045646680676	30.651422503523968	28.594157675369402	20.79396335429987
19	21.52243147118737	30.182353188298144	28.230018332747864	20.065197007766614
20	22.002502523571998	29.802064178891925	27.90080906569169	20.294624231844388
21	22.66718986590487	29.186472856506946	27.825141809041813	20.32119546854637
22	22.103635372252466	29.408525739007622	27.436110589686518	21.05172829905339
23	21.385252751869217	29.718936167360127	27.82321893840193	21.07259214236873
24	21.43090820356145	29.65520693483309	27.76766735969709	21.146217501908364
25	21.137701269624205	29.51328837585524	28.439314107978387	20.90969624654216
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	15766.0
1	15766.0
2	15747.0
3	15728.0
4	15728.0
5	15728.0
6	18121.0
7	20514.0
8	20514.0
9	20514.0
10	17900.0
11	15286.0
12	15286.0
13	15286.0
14	14513.0
15	13740.0
16	13740.0
17	13740.0
18	17437.0
19	21134.0
20	21134.0
21	21134.0
22	27229.0
23	33324.0
24	33324.0
25	33324.0
26	43954.0
27	54584.0
28	54584.0
29	54584.0
30	65105.5
31	75627.0
32	75627.0
33	75627.0
34	83220.5
35	90814.0
36	90814.0
37	90814.0
38	98293.5
39	105773.0
40	105773.0
41	105773.0
42	113460.0
43	121147.0
44	121147.0
45	121147.0
46	126029.5
47	130912.0
48	130912.0
49	130912.0
50	126443.0
51	121974.0
52	121974.0
53	121974.0
54	112402.0
55	102830.0
56	102830.0
57	102830.0
58	89578.5
59	76327.0
60	76327.0
61	76327.0
62	63027.0
63	49727.0
64	49727.0
65	49727.0
66	38943.0
67	28159.0
68	28159.0
69	28159.0
70	21732.0
71	15305.0
72	15305.0
73	15305.0
74	11298.5
75	7292.0
76	7292.0
77	7292.0
78	5586.5
79	3881.0
80	3881.0
81	3881.0
82	2666.5
83	1452.0
84	1452.0
85	1452.0
86	1066.0
87	680.0
88	680.0
89	680.0
90	479.0
91	278.0
92	278.0
93	278.0
94	161.5
95	45.0
96	45.0
97	45.0
98	35.0
99	25.0
100	25.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.35773983270428145
2	0.36157116839700476
3	0.3727978729849847
4	0.37502539373656807
5	0.380371443540368
6	0.31907007245679503
7	0.32789105463306495
8	0.32628723969192497
9	0.32593083637167164
10	0.4398907980226744
11	0.32405971894034163
12	0.32495072724097496
13	0.32013928241755496
14	0.323346912299835
15	0.31595154340457837
16	0.3211193915482517
17	0.31372402265299504
18	0.316931652535275
19	0.31728805585552833
20	0.5213289567005606
21	0.317733560005845
22	0.3202283832476183
23	0.315327837594135
24	0.317733560005845
25	0.3108727960909684
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1122324.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	63.935217562294525
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.39482693799329	56.515424916619736
2	6.345053176953935	8.113447106257556
3	2.0342864742349356	3.9018764494273093
4	0.9252789328311565	2.3663163950547075
5	0.5225062354571718	1.670327492080488
6	0.326296870331137	1.2517116836710231
7	0.2254684030042167	1.0090759979648387
8	0.1627200777607881	0.8322834858711575
9	0.12645231344993263	0.7276280554509215
>10	0.8089837970724217	9.91435342114627
>50	0.08086555951260357	3.578943662436551
>100	0.043625745115758875	4.968299453896609
>500	0.00195756415222999	0.8730614955699477
>1k	0.0011186080869885658	1.7575659433652646
>5k	4.1947803262071216E-4	1.519320102110512
>10k+	1.3982601087357072E-4	1.0003643390771775
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAA	11190	0.9970382884086948	No Hit
GTATCAACGCAGAGTACTTTTTTTT	6138	0.5469008949287372	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAA	5792	0.516072007726824	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	5065	0.45129570427078103	No Hit
TATCAACGCAGAGTACTTTTTTTTT	3593	0.32013928241755496	No Hit
GAACAAAAAAAAAAAAAAAAAAAAA	3307	0.29465644501944177	No Hit
NNNNNNNNNNNNNNNNNNNNNNNNN	3290	0.29314173090836515	No Hit
GGTATCAACGCAGAGTACTTTTTTT	2720	0.24235425777226538	No Hit
GAGTACTTTTTTTTTTTTTTTTTTT	2164	0.19281419625705232	No Hit
AAACAAAAAAAAAAAAAAAAAAAAA	2131	0.18987386886496235	No Hit
ACGCAGAGTACTTTTTTTTTTTTTT	1401	0.12483026291872935	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	8.910083006333287E-5	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATAACG	150	0.0	15.822108	2
CGCATAT	55	1.1286231E-5	15.544173	8
ATAACGC	165	0.0	15.534434	3
TAACGCA	165	0.0	14.975166	4
GACGTCT	70	4.3853834E-7	14.942727	15
GAACAAA	825	0.0	14.383093	1
GAGACCG	60	2.5430654E-5	14.264152	17
GAGTGCG	40	0.0052807597	14.248825	7
CGAGTGG	40	0.0052823285	14.248188	10
CGTCTCG	55	1.9429435E-4	13.831905	17
GTATAAC	225	0.0	13.50093	1
TGACCGA	155	0.0	13.497262	19
GGCGTAG	50	0.0015005245	13.298308	10
ATCGTCT	65	5.4015516E-5	13.166319	15
GCGTAGG	65	5.5162047E-5	13.136881	11
TTGACCG	160	0.0	13.075474	18
AGGACCG	95	7.348535E-8	13.004168	5
TTTGCGC	170	0.0	12.8657055	17
GGACGTC	60	4.0563112E-4	12.679246	14
CGGGGAT	60	4.094818E-4	12.665054	10
>>END_MODULE