FastQCFastQC Report
Thu 26 May 2016
SRR1364304_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364304_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1122324
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACTTTTTTTTTTTTTTTTTTTTT215511.9202119886948865No Hit
GTATCAACGCAGAGTACTTTTTTTT167721.4943991218222188No Hit
TATCAACGCAGAGTACTTTTTTTTT123111.096920318909691No Hit
GGTATCAACGCAGAGTACTTTTTTT119031.0605671802438512No Hit
GAGTACTTTTTTTTTTTTTTTTTTT94760.8443194656801424No Hit
ACGCAGAGTACTTTTTTTTTTTTTT51070.4550379391334409No Hit
GTGGTATCAACGCAGAGTACTTTTT26250.2338896789162488No Hit
GTATAACGCAGAGTACTTTTTTTTT22600.2013678759431323No Hit
ATCAACGCAGAGTACTTTTTTTTTT21090.18791365060356902No Hit
GCAGAGTACTTTTTTTTTTTTTTTT20900.1862207348323657No Hit
GTGGTATCAACGCAGAGTACATGGG15850.1412248156503826No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA15080.13436405173550597No Hit
ACTTTTTTTTTTTTTTTTTTTTTTT13090.11663298655290273No Hit
TGCATGCACTGCCTCAGTGACCAGT12480.11119783591903942No Hit
GTCCCAGGGTGTGCTTGTCAAAGAG12230.10897031516745609No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGACC350.002173267316.28170213
TATAACG3350.015.3144392
ATAACGC3650.014.83791353
TATCACG1300.014.6163442
CCGTGTG400.005278714514.2496659
TCGACGG400.00528652514.2464914
CGAAATC759.692685E-713.92990113
GCCCCCG1450.013.75523118
TAACGCA3800.013.7527424
ACAACGC1500.013.3020593
GTATAAC4150.013.2803481
GTACTTG1800.012.6697581
GTCCAAC604.0820366E-412.6697581
GACCTTC604.0881828E-412.66757
TGTGCCG604.091259E-412.66636910
TCGCGGT751.4807842E-512.6641117
GACGAAA905.416623E-712.66354711
TAGACGA853.9415863E-612.2938299
ACGAAAT853.9573315E-612.28944712
TAGGGTA701.08654014E-412.2189015
Produced by FastQC (version 0.11.5)