##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1364304_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1122324
Sequences flagged as poor quality	0
Sequence length	25
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.45299753012499	31.0	30.0	34.0	26.0	34.0
2	29.401037490065256	31.0	30.0	34.0	25.0	34.0
3	29.4572316015696	31.0	30.0	34.0	25.0	34.0
4	32.78572497781389	35.0	33.0	37.0	26.0	37.0
5	32.39091741778667	35.0	32.0	37.0	25.0	37.0
6	32.24866794259055	35.0	32.0	37.0	22.0	37.0
7	31.97309778637898	35.0	32.0	37.0	19.0	37.0
8	31.84351577619297	35.0	32.0	37.0	17.0	37.0
9	32.542594651811775	37.0	32.0	39.0	11.0	39.0
10	32.6700961576158	37.0	32.0	39.0	17.0	39.0
11	32.60721235579031	37.0	32.0	39.0	15.0	39.0
12	32.578762460751086	37.0	32.0	39.0	15.0	39.0
13	32.58647591960967	37.0	32.0	39.0	15.0	39.0
14	33.47475506181816	38.0	32.0	41.0	13.0	41.0
15	33.505018158749166	38.0	32.0	41.0	13.0	41.0
16	33.457490884985084	38.0	32.0	41.0	11.0	41.0
17	33.479636005289024	38.0	32.0	41.0	11.0	41.0
18	33.42815800071993	38.0	32.0	41.0	10.0	41.0
19	33.35797416788735	38.0	32.0	41.0	10.0	41.0
20	33.26831645763612	38.0	32.0	41.0	9.0	41.0
21	33.17582623199718	38.0	32.0	41.0	2.0	41.0
22	33.08381447781568	38.0	32.0	41.0	2.0	41.0
23	32.814824417904276	38.0	32.0	40.0	2.0	41.0
24	32.730376433186855	38.0	32.0	41.0	2.0	41.0
25	32.554243694334254	38.0	32.0	41.0	2.0	41.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1101	1	0.16279152285847687
1101	2	0.44477289863099756
1101	3	-1.472964283407702
1101	4	-0.5825648583232379
1101	5	0.40008106656485154
1101	6	0.6620518203067789
1101	7	0.7912116208427982
1101	8	0.801431738739133
1101	9	1.4264647596979714
1101	10	1.2269697717326409
1101	11	1.2085230409958783
1101	12	1.3806660934542947
1101	13	1.1639700894884157
1101	14	1.2033654860857084
1101	15	1.0350013703023322
1101	16	1.145056801993043
1101	17	1.030133045622648
1101	18	1.0351922715092527
1101	19	1.0498811503694796
1101	20	1.15455484785668
1101	21	1.05042525029112
1101	22	1.2514101595322842
1101	23	1.5716256379305946
1101	24	1.6827066997007947
1101	25	1.6703794986190061
1102	1	0.7158539043414791
1102	2	0.6390000211849056
1102	3	0.8644939301868106
1102	4	0.8070154204482876
1102	5	0.967113303188313
1102	6	1.1444223557592466
1102	7	1.2283394530325005
1102	8	1.2681531431533415
1102	9	1.70759392123297
1102	10	1.4398468268418227
1102	11	1.5289734544613651
1102	12	1.6945988051232064
1102	13	1.3220214256759348
1102	14	1.3631527908381145
1102	15	1.4726257520590238
1102	16	1.5031374619738855
1102	17	1.2804644044872333
1102	18	1.2307780631539273
1102	19	1.2830326761841917
1102	20	1.4317507112468988
1102	21	1.5508318735585718
1102	22	1.5843192753221658
1102	23	1.9604415431560938
1102	24	2.0932378513439005
1102	25	2.045413220981331
1103	1	-2.8430590120816674
1103	2	-2.4078341888287333
1103	3	-2.353682574781047
1103	4	-2.3335651367307477
1103	5	-4.9798377975325145
1103	6	-10.844960071671103
1103	7	-10.854984139408565
1103	8	-11.454114536566124
1103	9	-11.706140736861709
1103	10	-9.569601838655935
1103	11	-11.224504044077502
1103	12	-10.53641619585088
1103	13	-11.618812394702012
1103	14	-12.473375555158377
1103	15	-12.398658097541603
1103	16	-6.532260940519784
1103	17	-4.763057902156056
1103	18	-4.327433509026086
1103	19	-3.8987128917098275
1103	20	-8.228525930093443
1103	21	-5.205061366262196
1103	22	-11.962359080415219
1103	23	-12.70527051606853
1103	24	-13.809664934551286
1103	25	-14.223221105923013
1104	1	0.4557668577888272
1104	2	0.5586270375716431
1104	3	0.5095683223948022
1104	4	0.6786364547811914
1104	5	0.8644475824019509
1104	6	0.8510767515462447
1104	7	0.6033400921408223
1104	8	0.6832079786468555
1104	9	0.980109068100063
1104	10	0.8577035131930302
1104	11	0.8031796819327965
1104	12	0.7765382432191785
1104	13	0.6016198100101136
1104	14	0.8898285328428699
1104	15	0.7432127091365075
1104	16	0.6493820615086179
1104	17	0.7975560781840869
1104	18	0.8077279826262753
1104	19	0.8548563078532894
1104	20	1.0916722287456864
1104	21	0.9042536594828476
1104	22	0.5275613439879621
1104	23	1.005526800205459
1104	24	1.1598271859043265
1104	25	1.313723035128632
1105	1	0.3529652816810689
1105	2	0.3248704901180055
1105	3	-0.16747967391398078
1105	4	0.17756684073358997
1105	5	0.5260438502878415
1105	6	0.36807784737632687
1105	7	0.5748196724746002
1105	8	0.4112428774267656
1105	9	0.6610986446063052
1105	10	0.19706216422095935
1105	11	0.7123601426008861
1105	12	1.0527195375570386
1105	13	0.7173045989448497
1105	14	0.6572313893415611
1105	15	0.44061548698710595
1105	16	0.5247654709558205
1105	17	0.65172613108804
1105	18	0.6612786818078149
1105	19	0.8396097198696637
1105	20	0.8768882226216235
1105	21	1.0037251443063937
1105	22	0.8133711309564973
1105	23	1.2180522952951947
1105	24	1.3227041068839682
1105	25	1.2981238415337373
1106	1	-0.2552651249343292
1106	2	-0.09196428287262037
1106	3	-0.20399605440487178
1106	4	-1.4531380207432854
1106	5	-0.5343440671249908
1106	6	-0.13806351994696797
1106	7	0.13800175400836423
1106	8	0.28297402142760575
1106	9	0.7172722725426794
1106	10	0.1306724560149135
1106	11	0.22100927880651255
1106	12	0.3050218919234169
1106	13	0.05534512880895903
1106	14	-0.5943395050016349
1106	15	0.128869331925479
1106	16	0.07972965509150498
1106	17	0.1532430382828167
1106	18	0.21443561323096816
1106	19	-1.3073531738414879
1106	20	0.02135651504762137
1106	21	-0.24845870168539363
1106	22	0.16742148384810207
1106	23	0.22268794788131174
1106	24	0.2953115544769318
1106	25	0.556128423857551
1107	1	-0.3632037885240216
1107	2	-0.3546367145481888
1107	3	-0.4577091695840423
1107	4	-0.5097439041639191
1107	5	-0.3220255741519047
1107	6	-0.207487364784388
1107	7	-0.3443875267052192
1107	8	-0.6380396304937754
1107	9	0.22405577355393547
1107	10	0.05368041639452059
1107	11	-0.0664223319387034
1107	12	-0.1274856924773502
1107	13	-0.2978847239110536
1107	14	0.033936048594796375
1107	15	-0.011184407119273487
1107	16	-0.23943878342947755
1107	17	-0.5447759421662823
1107	18	-0.3802297007761908
1107	19	-0.14060364057384334
1107	20	-0.24406185864941676
1107	21	-0.29730127026911646
1107	22	-0.19460724612130065
1107	23	-0.08231872898431902
1107	24	0.18006301949792913
1107	25	-0.30874542875874056
1108	1	-0.04150617298093451
1108	2	0.07848825841325535
1108	3	0.022611288869139656
1108	4	-0.10515588456670599
1108	5	0.36617367644830523
1108	6	0.30173659763092786
1108	7	-0.0832190519346625
1108	8	0.3385145457181302
1108	9	0.6901535322294947
1108	10	0.3693509071933647
1108	11	0.43784062133229895
1108	12	0.6031690379872288
1108	13	0.30666754610359703
1108	14	0.34911020372585
1108	15	-0.05869580355631854
1108	16	-0.5933730652334432
1108	17	-0.5208150024418927
1108	18	-0.5004757357081573
1108	19	-0.29547747238245137
1108	20	0.3015760383227004
1108	21	0.18711362208137672
1108	22	0.17132293219399486
1108	23	-0.16976401887620796
1108	24	0.1468434073976681
1108	25	0.013564557682578027
1109	1	0.310149880942312
1109	2	0.33008909236372475
1109	3	0.14374527116107672
1109	4	0.20520712974983724
1109	5	0.1777982242542322
1109	6	0.43073917819805985
1109	7	0.46386217099441396
1109	8	0.2399483813846821
1109	9	1.3398983009549639
1109	10	0.5219335066562323
1109	11	0.412006199957311
1109	12	0.6038679622387093
1109	13	0.7070777799408532
1109	14	0.18539438533512254
1109	15	0.17622899516836554
1109	16	0.46152867394786057
1109	17	0.364510859837317
1109	18	0.28827465712692657
1109	19	0.6015100153863742
1109	20	0.7480319966767297
1109	21	0.7302451794531422
1109	22	0.4494491807155967
1109	23	0.7956308039291429
1109	24	0.4044886968960597
1109	25	0.4733655080212813
1110	1	-0.0032080234485576398
1110	2	0.10644606716515526
1110	3	-0.017279862010006752
1110	4	0.27518450506885017
1110	5	0.007821004645343521
1110	6	0.10376473490067895
1110	7	0.22722046466059354
1110	8	0.23668687713146142
1110	9	-3.0833305353142855
1110	10	-1.2892312206408434
1110	11	-0.32057816768052305
1110	12	0.09032943410422689
1110	13	-0.1329780265675211
1110	14	0.12861126306685833
1110	15	0.19924046010478236
1110	16	-0.23664531343028017
1110	17	-0.24824273356306747
1110	18	0.0073262579048503085
1110	19	-0.3287253093237368
1110	20	0.19575528110151197
1110	21	-0.077904999847064
1110	22	0.22952171049126946
1110	23	0.47654245556199726
1110	24	0.3858838284633279
1110	25	0.601919786234788
1111	1	-0.042381497346699604
1111	2	0.04760453133418352
1111	3	-0.10585708377867675
1111	4	-0.06032305249177483
1111	5	0.055240234150112144
1111	6	0.02383312968392204
1111	7	0.18859219848722475
1111	8	0.3170891290627331
1111	9	0.9938690538136541
1111	10	0.3432924807942186
1111	11	0.5261822822652888
1111	12	0.5750134385652999
1111	13	0.4634096969778696
1111	14	0.44732780695954233
1111	15	0.33991128897467604
1111	16	0.26237768565265185
1111	17	0.1881767020069347
1111	18	0.41289819905513525
1111	19	0.2810047452127691
1111	20	0.5517595660000723
1111	21	0.4979208734526992
1111	22	0.5121636241609409
1111	23	0.7589641468385153
1111	24	0.6385443509252298
1111	25	1.0151309276002607
1112	1	0.0523596231146648
1112	2	-0.011111913241112603
1112	3	-0.019131203508184313
1112	4	0.1371137364534647
1112	5	0.0902960951572993
1112	6	0.168872239006987
1112	7	0.35398255404971124
1112	8	-0.06556304767157073
1112	9	0.45855315269152186
1112	10	0.3424837899339792
1112	11	0.4100884947866632
1112	12	-0.11593041827349282
1112	13	0.00781715578709452
1112	14	-0.5865942726898865
1112	15	0.006274171968897235
1112	16	-0.17020961825596004
1112	17	-0.1386945295501505
1112	18	-0.2025584820711117
1112	19	0.25995876734410217
1112	20	0.04236858285474199
1112	21	-0.2640211984552536
1112	22	0.13667054064690376
1112	23	0.2304296270378572
1112	24	0.23764642966427374
1112	25	0.11365917480075183
1113	1	-2.6311790460244247
1113	2	-2.778843985943599
1113	3	-2.8555417123263673
1113	4	-2.730587430952763
1113	5	-2.7528995887845404
1113	6	-2.5458934975756016
1113	7	-3.076476078003026
1113	8	-2.935623446540667
1113	9	-11.866936848872818
1113	10	-5.156873303594605
1113	11	-3.7653047846468155
1113	12	-14.177224650683865
1113	13	-5.9603999901660245
1113	14	-4.981066676040889
1113	15	-4.599846094147328
1113	16	-3.9549408757199664
1113	17	-4.093675814676306
1113	18	-4.37125052669175
1113	19	-4.76834183562713
1113	20	-15.558557558574506
1113	21	-17.144720395807823
1113	22	-12.587307009135422
1113	23	-18.363605777967784
1113	24	-19.786275287093133
1113	25	-16.308247720132684
1114	1	-0.34413732556834375
1114	2	-0.4226435445558181
1114	3	-0.27553199877545964
1114	4	-0.8522106508690648
1114	5	-0.5519800728515492
1114	6	-0.5071298866511285
1114	7	-0.5911231565141826
1114	8	-0.6015137840739797
1114	9	0.20521646417781625
1114	10	-0.6046315116709451
1114	11	-0.6837298305093711
1114	12	-0.7111805572022618
1114	13	-0.5504868836667534
1114	14	-0.562972545189794
1114	15	-0.5642645519072218
1114	16	-0.7422178855568262
1114	17	-0.8572590580460542
1114	18	-0.7873264534425175
1114	19	-1.1123198115345048
1114	20	-1.348319576577289
1114	21	-1.693486950995137
1114	22	-1.587001258763614
1114	23	-1.1305837312269809
1114	24	-0.8847822690224056
1114	25	-1.0122285144797019
1115	1	-0.04422913078663271
1115	2	0.04387301812425548
1115	3	0.07972412612382129
1115	4	0.13744714763443255
1115	5	-0.12909001266881148
1115	6	0.048679524242885464
1115	7	-0.3166881482879198
1115	8	0.16655254396889418
1115	9	0.5636148926884914
1115	10	0.24029713984172218
1115	11	0.3862602955202945
1115	12	0.24022281688498026
1115	13	-0.03689467925531886
1115	14	-0.033260424507659536
1115	15	-0.0031128259875288222
1115	16	0.104253535466448
1115	17	0.028217000490819544
1115	18	-0.016900559461589637
1115	19	-0.04539159818310168
1115	20	-0.3485816141990199
1115	21	0.02068124524710413
1115	22	-0.3794217438461871
1115	23	-0.025585808504146712
1115	24	0.5264112172255295
1115	25	0.5442074547557354
1116	1	-0.4162432646420342
1116	2	-0.3298110750450256
1116	3	-0.3567456209146549
1116	4	-0.24167375272247682
1116	5	-1.2376974235778029
1116	6	-0.9323414491022817
1116	7	-2.1705906413347122
1116	8	-1.3896402342077643
1116	9	-0.21541443788585823
1116	10	-1.355000692362598
1116	11	-0.9399038373688668
1116	12	-1.9984475779799773
1116	13	-1.6317786636111542
1116	14	-0.927739926106419
1116	15	-2.4071707349558658
1116	16	-2.799159371413328
1116	17	-3.180891219829647
1116	18	-2.332187807626106
1116	19	-2.102245730195218
1116	20	-2.3568544089058108
1116	21	-1.5519560388169253
1116	22	-1.3198710601201888
1116	23	-1.511001982760252
1116	24	-2.0537502488423662
1116	25	-1.6232962726992959
1201	1	0.9046532271449195
1201	2	0.8567084547189587
1201	3	0.4757119220681467
1201	4	0.8785840667605811
1201	5	1.144155313743859
1201	6	1.1327650839122825
1201	7	1.4581944585796158
1201	8	1.1803369096744305
1201	9	2.015327813766497
1201	10	1.5761024941946644
1201	11	1.6068318203398917
1201	12	1.726083314893181
1201	13	1.6814537605291093
1201	14	1.6365074148208762
1201	15	1.8390224379000983
1201	16	1.702480145236997
1201	17	1.618076388814238
1201	18	1.5560844368817754
1201	19	1.621816287514406
1201	20	1.832979412581416
1201	21	1.896120676087797
1201	22	1.7969230832702507
1201	23	2.244688386432543
1201	24	2.1936463496477074
1201	25	2.0990380823035295
1202	1	0.6378649241504419
1202	2	0.6780313710797259
1202	3	0.6209809371075536
1202	4	0.8468894616168754
1202	5	0.7365463613441747
1202	6	0.7971445790406619
1202	7	0.9488093519222609
1202	8	0.8118720426976722
1202	9	1.4892434608032374
1202	10	0.8925563398476157
1202	11	1.0873612831014299
1202	12	1.1071546508967245
1202	13	1.0282532738135615
1202	14	1.068636003741588
1202	15	1.1055892163717473
1202	16	1.013687626254196
1202	17	1.1033708632137902
1202	18	0.9803900819905564
1202	19	1.1553967238985692
1202	20	1.4250212558186632
1202	21	1.4465580433805627
1202	22	1.305574669245388
1202	23	1.5679539515626715
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2211	4	0.18171005504701299
2211	5	-0.6543592840894519
2211	6	-0.08395984824070268
2211	7	0.10250450287936275
2211	8	0.092939905975026
2211	9	0.7205107861888607
2211	10	0.030777993026497086
2211	11	0.448162970147564
2211	12	0.4977165218075683
2211	13	0.3967904949978447
2211	14	0.468621912630411
2211	15	0.4028053715877604
2211	16	0.2050928987592826
2211	17	0.17142485646463257
2211	18	0.04182246773715548
2211	19	-0.13130393959841058
2211	20	0.4515180112188375
2211	21	0.6432517342070057
2211	22	0.4527150240012574
2211	23	0.9025905102217777
2211	24	0.8702747392403296
2211	25	1.049602309583058
2212	1	-0.03208203130428444
2212	2	-0.08145884096017753
2212	3	0.031879267493970076
2212	4	0.01876719523261272
2212	5	-0.08772036079390944
2212	6	0.06348645332935376
2212	7	0.0032955929860634114
2212	8	-0.1742605031338833
2212	9	0.5353638983140243
2212	10	0.27500642909688366
2212	11	0.3099842435798408
2212	12	0.14816332091131557
2212	13	-0.06342066245483124
2212	14	-0.008689512289912216
2212	15	-0.017265148906354
2212	16	0.18630003553948882
2212	17	-0.26764515956957524
2212	18	0.11752493104837214
2212	19	0.19553875317858171
2212	20	0.48282886818886794
2212	21	0.47231197272304115
2212	22	0.15945296919866792
2212	23	0.48481776421676415
2212	24	0.6122853409790423
2212	25	0.5404568092615634
2213	1	-0.08218065647119843
2213	2	-0.19401862104259493
2213	3	-0.19831547005978933
2213	4	-0.05885623710889831
2213	5	-0.0695491307909677
2213	6	0.08788653434184823
2213	7	-0.22390847203052644
2213	8	-0.012199826168878047
2213	9	0.5984494761750909
2213	10	-0.0019242767854024123
2213	11	0.1632416596178814
2213	12	0.10269796042602763
2213	13	-0.23763002171912007
2213	14	-0.01624874397113274
2213	15	-0.13883930986703774
2213	16	-0.32377690891994604
2213	17	-0.44638187534861373
2213	18	-0.48839048981907496
2213	19	-0.4723584793394622
2213	20	-0.05939193031808543
2213	21	-0.358283126717474
2213	22	-0.1406922265457382
2213	23	-0.47581602507298726
2213	24	-0.5040900347047916
2213	25	-0.056314819652463655
2214	1	-0.9195627060082288
2214	2	-0.6488688549087698
2214	3	-0.7566010517467099
2214	4	-0.5262872764583335
2214	5	-0.8904246093897434
2214	6	-0.6877179590750906
2214	7	-0.545750239612051
2214	8	-0.4053400402417182
2214	9	-10.061292985298895
2214	10	-3.503411807674617
2214	11	-1.298092927466751
2214	12	-0.9490617072259724
2214	13	-0.6768152911804073
2214	14	-0.7078363152089722
2214	15	-0.764256000344588
2214	16	-0.9458817218356614
2214	17	-0.7825889659702412
2214	18	-1.110069304366057
2214	19	-1.1032629292907146
2214	20	-0.8793279612947487
2214	21	-1.4986946134344628
2214	22	-1.1994046724517347
2214	23	-1.4353297288863658
2214	24	-1.7246844582822405
2214	25	-9.614146695759672
2215	1	-0.2552895561260726
2215	2	-0.15802998398518042
2215	3	-0.06814860527186539
2215	4	-0.2307859860635233
2215	5	0.21534145958644757
2215	6	-0.0027823733096994374
2215	7	-0.24796973040578862
2215	8	-0.23624205053791414
2215	9	0.6393749837785236
2215	10	0.5832020240213254
2215	11	0.6576804976097534
2215	12	0.6994960575042484
2215	13	0.4712723865016528
2215	14	0.3578718310713285
2215	15	0.11333673194553029
2215	16	0.19755314912778488
2215	17	0.19623191269396045
2215	18	0.2528044866398105
2215	19	0.3532355552353863
2215	20	0.5716033327282872
2215	21	0.36412085629796564
2215	22	0.5444386232687748
2215	23	0.5482937161483292
2215	24	0.8886850635477899
2215	25	1.1395816161999406
2216	1	-0.35145318633004763
2216	2	-0.2618796416723441
2216	3	-0.3828974461727128
2216	4	-0.21792246946503013
2216	5	-0.8605371872513352
2216	6	-1.1379549423139572
2216	7	-0.41771954694909397
2216	8	-1.2266406513588883
2216	9	0.06261885728258676
2216	10	-0.5051816057756326
2216	11	-0.21569427957864207
2216	12	-1.230635372907301
2216	13	-1.1665116720991442
2216	14	-0.970646138107945
2216	15	-0.41354072137626474
2216	16	-1.4459267460534981
2216	17	-1.8616913216026845
2216	18	-1.3836130739667425
2216	19	-2.6395680341711696
2216	20	-1.5811074569196073
2216	21	-1.6937880127109572
2216	22	-1.365447926871468
2216	23	-0.9893882291989371
2216	24	-1.1734711421966466
2216	25	-1.2484972300759196
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	45219.0
3	2.0
4	658.0
5	6203.0
6	8672.0
7	6647.0
8	5230.0
9	4118.0
10	3538.0
11	3159.0
12	2885.0
13	2667.0
14	2669.0
15	2684.0
16	2771.0
17	2959.0
18	3351.0
19	4329.0
20	5955.0
21	7677.0
22	9392.0
23	11014.0
24	12473.0
25	14266.0
26	15867.0
27	17002.0
28	18797.0
29	22096.0
30	26742.0
31	32889.0
32	40599.0
33	49903.0
34	62601.0
35	79127.0
36	103559.0
37	157312.0
38	327292.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	47.108220262244124	17.959764992762974	12.025493579368419	22.90652116562449
2	16.24006180105158	22.081134373627272	38.87282580318646	22.805978022134685
3	18.407244286161063	29.474774997126485	29.131913475580472	22.986067241131977
4	11.645222226182414	17.4334390704637	36.22268952489575	34.69864917845814
5	11.842282354765764	39.36202736536804	34.2000251352804	14.595665144585798
6	28.87508632789004	37.41288123510147	19.194065096798628	14.51796734020986
7	26.219707826235748	33.133078804212204	23.26962975637927	17.377583613172774
8	26.50589845880705	33.53966352581311	21.483652231574666	18.470785783805173
9	27.134190907762022	15.101735946463396	20.805865637957652	36.95820750781693
10	15.586729668562608	30.07689832431715	32.38046085512505	21.955911151995195
11	32.917914246161885	23.488664594473896	24.364013017910768	19.22940814145345
12	23.14836761935653	27.605313011817582	30.543758565565227	18.702560803260663
13	28.395981377113454	21.73816577933216	27.953843754872914	21.912009088681472
14	21.755925171486464	21.183412549476675	30.634014289202188	26.426647989834674
15	23.26535267694441	28.44147060631902	26.688177389503586	21.604999327232985
16	21.345928673140772	28.17599851721686	28.15300824976341	22.325064559878953
17	20.967085101813673	27.60192729084109	29.655933851186557	21.775053756158677
18	20.13410258740997	25.130867656573326	33.398744882482	21.3362848735347
19	21.646056721610677	25.06220412935607	33.10311270176239	20.18862644727086
20	21.790724491996528	24.570774717548108	33.197877622894865	20.440623167560496
21	22.416842885696898	23.979244027043716	33.10235496349526	20.501558123764127
22	21.843627193256044	24.119266790828647	33.08316773451911	20.953938281396198
23	21.129176557810617	24.318491270842692	33.62338444648802	20.928947724858673
24	21.397046440196785	24.46813913970197	33.23828524939875	20.896529170702497
25	21.00031220730565	24.397841309486644	33.714285714285715	20.887560768921993
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2187.0
1	2187.0
2	2961.5
3	3736.0
4	3736.0
5	3736.0
6	14208.5
7	24681.0
8	24681.0
9	24681.0
10	19674.0
11	14667.0
12	14667.0
13	14667.0
14	11455.0
15	8243.0
16	8243.0
17	8243.0
18	13099.0
19	17955.0
20	17955.0
21	17955.0
22	25679.5
23	33404.0
24	33404.0
25	33404.0
26	47907.0
27	62410.0
28	62410.0
29	62410.0
30	71447.0
31	80484.0
32	80484.0
33	80484.0
34	87983.0
35	95482.0
36	95482.0
37	95482.0
38	98947.0
39	102412.0
40	102412.0
41	102412.0
42	109470.5
43	116529.0
44	116529.0
45	116529.0
46	124084.0
47	131639.0
48	131639.0
49	131639.0
50	128684.0
51	125729.0
52	125729.0
53	125729.0
54	116787.0
55	107845.0
56	107845.0
57	107845.0
58	94112.0
59	80379.0
60	80379.0
61	80379.0
62	66915.5
63	53452.0
64	53452.0
65	53452.0
66	41755.0
67	30058.0
68	30058.0
69	30058.0
70	23402.0
71	16746.0
72	16746.0
73	16746.0
74	12017.5
75	7289.0
76	7289.0
77	7289.0
78	5815.5
79	4342.0
80	4342.0
81	4342.0
82	2989.0
83	1636.0
84	1636.0
85	1636.0
86	1122.0
87	608.0
88	608.0
89	608.0
90	496.0
91	384.0
92	384.0
93	384.0
94	203.5
95	23.0
96	23.0
97	23.0
98	13.5
99	4.0
100	4.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.028155862300013185
2	0.0011583107908233272
3	4.455041503166644E-4
4	0.003920436522786647
5	0.03519482787501649
6	0.013276023679436599
7	0.022453409175959883
8	0.029937878901279844
9	0.03564033202533315
10	0.040362676018689786
11	0.04321390258071644
12	0.008731881346206621
13	0.0043659406731033105
14	0.006949864744939964
15	0.008286377195889956
16	0.009801091306966617
17	0.011315805418043274
18	0.017641964352539907
19	0.01888937597342657
20	0.016572754391779913
21	0.012563217038929935
22	0.011939511228486605
23	0.00962288964683995
24	0.007573570555383293
25	0.11351445750068608
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1122324.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	43.2207743475263
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.01486540143674	32.854213445730814
2	12.182570241536276	10.530802387646567
3	4.494991892172654	5.828310907966637
4	2.150442006177733	3.717750747857982
5	1.203741164875424	2.6013312629954575
6	0.765438221401001	1.9849699586486726
7	0.5134175245824213	1.5533212083229657
8	0.3620071018192523	1.2516981807945504
9	0.280329125998554	1.0904437708040493
>10	1.725886671498901	14.468396295546738
>50	0.18589913528420474	5.577425139555346
>100	0.11173692749372847	8.56402909866806
>500	0.00536998163814039	1.5792263388893224
>1k	0.0020653775531309193	1.5137934687064116
>5k	4.130755106261838E-4	1.3017838279364076
>10k+	8.261510212523676E-4	5.582503959929996
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GTACTTTTTTTTTTTTTTTTTTTTT	21551	1.9202119886948865	No Hit
GTATCAACGCAGAGTACTTTTTTTT	16772	1.4943991218222188	No Hit
TATCAACGCAGAGTACTTTTTTTTT	12311	1.096920318909691	No Hit
GGTATCAACGCAGAGTACTTTTTTT	11903	1.0605671802438512	No Hit
GAGTACTTTTTTTTTTTTTTTTTTT	9476	0.8443194656801424	No Hit
ACGCAGAGTACTTTTTTTTTTTTTT	5107	0.4550379391334409	No Hit
GTGGTATCAACGCAGAGTACTTTTT	2625	0.2338896789162488	No Hit
GTATAACGCAGAGTACTTTTTTTTT	2260	0.2013678759431323	No Hit
ATCAACGCAGAGTACTTTTTTTTTT	2109	0.18791365060356902	No Hit
GCAGAGTACTTTTTTTTTTTTTTTT	2090	0.1862207348323657	No Hit
GTGGTATCAACGCAGAGTACATGGG	1585	0.1412248156503826	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA	1508	0.13436405173550597	No Hit
ACTTTTTTTTTTTTTTTTTTTTTTT	1309	0.11663298655290273	No Hit
TGCATGCACTGCCTCAGTGACCAGT	1248	0.11119783591903942	No Hit
GTCCCAGGGTGTGCTTGTCAAAGAG	1223	0.10897031516745609	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	2.673024901899986E-4	0.0	0.0	0.0	0.0
3	2.673024901899986E-4	0.0	0.0	0.0	0.0
4	2.673024901899986E-4	0.0	0.0	0.0	0.0
5	3.564033202533315E-4	0.0	0.0	0.0	0.0
6	3.564033202533315E-4	0.0	0.0	0.0	0.0
7	3.564033202533315E-4	0.0	0.0	0.0	0.0
8	3.564033202533315E-4	0.0	0.0	0.0	0.0
9	3.564033202533315E-4	0.0	0.0	0.0	0.0
10	3.564033202533315E-4	0.0	0.0	0.0	0.0
11	3.564033202533315E-4	0.0	0.0	0.0	0.0
12	3.564033202533315E-4	0.0	0.0	0.0	0.0
13	3.564033202533315E-4	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCCGACC	35	0.0021732673	16.281702	13
TATAACG	335	0.0	15.314439	2
ATAACGC	365	0.0	14.8379135	3
TATCACG	130	0.0	14.616344	2
CCGTGTG	40	0.0052787145	14.249665	9
TCGACGG	40	0.005286525	14.24649	14
CGAAATC	75	9.692685E-7	13.929901	13
GCCCCCG	145	0.0	13.755231	18
TAACGCA	380	0.0	13.752742	4
ACAACGC	150	0.0	13.302059	3
GTATAAC	415	0.0	13.280348	1
GTACTTG	180	0.0	12.669758	1
GTCCAAC	60	4.0820366E-4	12.669758	1
GACCTTC	60	4.0881828E-4	12.6675	7
TGTGCCG	60	4.091259E-4	12.666369	10
TCGCGGT	75	1.4807842E-5	12.66411	17
GACGAAA	90	5.416623E-7	12.663547	11
TAGACGA	85	3.9415863E-6	12.293829	9
ACGAAAT	85	3.9573315E-6	12.289447	12
TAGGGTA	70	1.08654014E-4	12.218901	5
>>END_MODULE