FastQCFastQC Report
Thu 26 May 2016
SRR1364303_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364303_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1983313
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNN52390.26415396863732554No Hit
GTATCAACGCAGAGTACTTTTTTTT46270.23329650942639918No Hit
GTACTTTTTTTTTTTTTTTTTTTTT32990.16633783976608837No Hit
TATCAACGCAGAGTACTTTTTTTTT30770.15514444769937977No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAACGCA1950.015.1083234
ATAACGC2100.013.56080153
TATAACG2100.013.1087752
TGCGTAG604.0588796E-412.67920514
ACGGGTT604.0797566E-412.6714965
GGACCGA1001.4441139E-712.3493916
TATCACG701.0991968E-412.2047222
CGAAATC550.003096966512.07624713
TCGTCGC550.003096966512.07624713
GTATAAC3900.011.9265521
ACGAAAT658.1106543E-411.67753912
GTTATCA3750.011.6442081
AGCGTTC907.5690605E-611.59644412
GACGTCT1156.98019E-811.57666515
GTATTAG1751.8189894E-1211.3910741
GGTATCA16700.011.3683371
TCCCGAC1354.78758E-911.25044252
CGACCCG1104.9457594E-711.2315535
CGCATAT1104.9749906E-711.2264358
CGGTTTG855.2709296E-511.18753314
Produced by FastQC (version 0.11.5)