##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1364303_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1983313
Sequences flagged as poor quality	0
Sequence length	25
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	26.416146619318283	30.0	26.0	31.0	10.0	34.0
2	26.295819167221715	31.0	26.0	31.0	10.0	34.0
3	25.682224641294642	30.0	25.0	31.0	7.0	34.0
4	29.504705006219393	35.0	28.0	37.0	10.0	37.0
5	30.06661681741611	35.0	30.0	37.0	10.0	37.0
6	30.217012140796736	35.0	30.0	37.0	7.0	37.0
7	29.858539726205596	35.0	30.0	37.0	2.0	37.0
8	29.338045482483096	35.0	29.0	37.0	2.0	37.0
9	30.43883945700956	35.0	29.0	39.0	2.0	39.0
10	29.709223909690504	35.0	27.0	39.0	2.0	39.0
11	30.422733073397897	35.0	29.0	39.0	2.0	39.0
12	29.905166758852484	35.0	27.0	39.0	2.0	39.0
13	29.542849262824376	35.0	27.0	39.0	2.0	39.0
14	29.84716986174144	36.0	27.0	40.0	2.0	41.0
15	30.557606893112684	36.0	27.0	40.0	2.0	41.0
16	30.93204804284548	37.0	29.0	40.0	2.0	41.0
17	31.102953996671225	37.0	30.0	40.0	2.0	41.0
18	30.9365813666325	37.0	29.0	40.0	2.0	41.0
19	30.921640205050842	37.0	29.0	40.0	2.0	41.0
20	30.76741139699079	37.0	29.0	40.0	2.0	41.0
21	30.706191609695495	37.0	28.0	40.0	2.0	41.0
22	30.771907913677772	37.0	29.0	40.0	2.0	41.0
23	30.769517469002622	37.0	30.0	40.0	2.0	41.0
24	30.59990178050565	37.0	30.0	40.0	2.0	41.0
25	30.41272708846259	37.0	29.0	40.0	2.0	41.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1101	1	0.29576499540560874
1101	2	0.43852809277022686
1101	3	1.1639072559759356
1101	4	0.5690930793359144
1101	5	-0.05554312795296923
1101	6	-0.10444544844271775
1101	7	0.19168623402538998
1101	8	0.5600181809921665
1101	9	0.38631300406540703
1101	10	0.697453877342614
1101	11	0.1620851436575954
1101	12	0.570952150711193
1101	13	0.33907428143550433
1101	14	0.90542252516817
1101	15	0.12015305045357039
1101	16	-0.3843967222043254
1101	17	-0.907116244017331
1101	18	-6.84702608137194
1101	19	-8.08806943524171
1101	20	-9.349837547939739
1101	21	-10.88364481896594
1101	22	-11.110698887468782
1101	23	-12.46937788507346
1101	24	-12.263589843134625
1101	25	-12.578800459636415
1102	1	1.953793725600299
1102	2	2.183765934185594
1102	3	2.8521282315055174
1102	4	2.2364857741908644
1102	5	1.7492159213032323
1102	6	1.6343181048978117
1102	7	1.991395067627458
1102	8	2.268140635229514
1102	9	2.4964902802048634
1102	10	-1.1084491826807508
1102	11	1.154457516830309
1102	12	2.1459342720727257
1102	13	2.626172567307158
1102	14	3.278316954584195
1102	15	2.933307064374482
1102	16	2.4894065194926576
1102	17	2.3690547512787035
1102	18	2.481960188722095
1102	19	2.104768893005648
1102	20	2.5963466397926283
1102	21	2.664584418493469
1102	22	2.4886568152616526
1102	23	2.500691275274608
1102	24	2.810782849854725
1102	25	2.756713401305536
1103	1	-1.614760902788305
1103	2	-0.9258612294041271
1103	3	-3.628451704217312
1103	4	-2.387933785669052
1103	5	-3.83914789324416
1103	6	-8.624783744151724
1103	7	-9.78971955038456
1103	8	-4.3742592379455765
1103	9	-9.703529896485293
1103	10	-5.7851508703167625
1103	11	-9.886370015704603
1103	12	-4.302120839115251
1103	13	-1.610791129062477
1103	14	-1.5840174884670972
1103	15	-1.9955542497045258
1103	16	-1.9644407429628465
1103	17	-1.746540389021355
1103	18	-1.7178510151057118
1103	19	-8.39195960080443
1103	20	-3.8049028738050197
1103	21	-8.186474137143751
1103	22	-4.577952203718851
1103	23	-3.412391867100766
1103	24	-8.988482142146655
1103	25	-5.116896929159825
1104	1	1.8184817051896225
1104	2	1.6418843359538258
1104	3	2.7373980534517415
1104	4	2.091268878400566
1104	5	1.665381710544029
1104	6	1.506800389097453
1104	7	1.7155925952938915
1104	8	2.1248596884297974
1104	9	2.2883562293897093
1104	10	2.515803602309976
1104	11	2.154002291451654
1104	12	2.3512730793347423
1104	13	2.6328321541291544
1104	14	3.1687049861931094
1104	15	2.1912704866159842
1104	16	1.399446947995937
1104	17	1.111978036412971
1104	18	1.1396749530042776
1104	19	1.6629725550984773
1104	20	-9.245111247003248
1104	21	-3.309607389176506
1104	22	-0.5972344357339523
1104	23	0.5184359993873038
1104	24	0.9959669797785118
1104	25	1.498273816339843
1105	1	1.173372722239293
1105	2	1.1141525907107344
1105	3	1.836586559150497
1105	4	1.0500448648340068
1105	5	0.9582741679026796
1105	6	0.8457524591014511
1105	7	1.2413427347513597
1105	8	1.438780699892579
1105	9	1.502779760104925
1105	10	1.8834774689469853
1105	11	1.4469456362922877
1105	12	1.6616787749537423
1105	13	1.5894715104647723
1105	14	2.3649986168410315
1105	15	1.8492346781470204
1105	16	1.3039461743773835
1105	17	0.8002730996401297
1105	18	1.0949968174013769
1105	19	1.1790089828058328
1105	20	1.2189095855159593
1105	21	1.107445585765813
1105	22	1.0325728746285847
1105	23	1.0066500720735228
1105	24	0.8651479317995587
1105	25	0.8910238316919745
1106	1	1.1200963980839056
1106	2	1.3991335857264708
1106	3	1.9844113806647634
1106	4	1.522975755642836
1106	5	0.806100462000046
1106	6	0.7716873761257261
1106	7	1.2979724338073488
1106	8	1.1394619428220132
1106	9	1.5995228393974585
1106	10	-2.010518419947484
1106	11	0.22375661032804928
1106	12	0.956288731001468
1106	13	1.811702274631454
1106	14	0.9137486969668771
1106	15	1.6114038450287964
1106	16	1.1289632390168514
1106	17	-5.858827377303552
1106	18	-1.6410069298894996
1106	19	-0.25732150468847337
1106	20	0.5832911785736812
1106	21	0.9757633068308529
1106	22	1.165634400883011
1106	23	0.6914956327552098
1106	24	0.9570392828858942
1106	25	1.1956196336174187
1107	1	0.9781937654743729
1107	2	1.2748249231634112
1107	3	1.6379422789706766
1107	4	1.1792015202701798
1107	5	0.6380146290401996
1107	6	0.8552791129830304
1107	7	0.7108796630502994
1107	8	0.91371777495063
1107	9	0.8792998375490377
1107	10	-2.4625613038636374
1107	11	-0.27772375586013
1107	12	0.23520696679916497
1107	13	1.1293842525549316
1107	14	1.664127786398133
1107	15	1.2636874651946854
1107	16	-5.31415588611458
1107	17	-1.8709569536917314
1107	18	-0.04596752772606649
1107	19	0.7008964986210344
1107	20	1.1639621379452372
1107	21	1.2104150587189082
1107	22	0.9875535253288916
1107	23	0.5368104344461244
1107	24	0.7800952471548683
1107	25	1.0662978641363594
1108	1	-0.23873048895484317
1108	2	-2.911893756906995
1108	3	0.12502542573597353
1108	4	-0.4555683307915821
1108	5	-0.6647917356363173
1108	6	-0.19750187986655376
1108	7	0.04997228524385022
1108	8	0.36526336034364704
1108	9	-0.1372332897155104
1108	10	-3.096186668974653
1108	11	-1.2492626082279656
1108	12	-5.72664343021512
1108	13	-1.6242912936986293
1108	14	-6.030638708845171
1108	15	-2.595022525252247
1108	16	-7.810239213931524
1108	17	-3.277400681225366
1108	18	-1.7231524430547474
1108	19	-0.33497536130124317
1108	20	-0.24693441205308986
1108	21	0.1371660329275528
1108	22	0.02128313683354932
1108	23	-0.01813788455939047
1108	24	0.2668491216119655
1108	25	-0.006812137945789942
1109	1	-9.35386519591145
1109	2	-6.67045934425003
1109	3	-5.759035190312169
1109	4	-5.540123414105487
1109	5	-2.8377076701913637
1109	6	-1.6719839085888424
1109	7	-1.2227484792920755
1109	8	-0.5613987452214992
1109	9	-5.661577457404629
1109	10	-1.659822100952404
1109	11	-1.1440245215752611
1109	12	-6.075405959933185
1109	13	-7.916907043247555
1109	14	-7.903639339027876
1109	15	-3.7884319971397424
1109	16	-2.0991868931627273
1109	17	-1.759554132664448
1109	18	-1.2090062278042844
1109	19	-0.8879947925848093
1109	20	-0.7889122030945437
1109	21	-0.7060562766999681
1109	22	-0.7120425630335276
1109	23	-1.1126213239968479
1109	24	-0.6966132499858055
1109	25	-0.6003112925183167
1110	1	-2.538711909554312
1110	2	-2.344134828735662
1110	3	-1.6104067179161667
1110	4	-2.3831298785648123
1110	5	-5.316314856592996
1110	6	-4.148599899564459
1110	7	-8.951084775557366
1110	8	-9.739448560696708
1110	9	-11.483604988557882
1110	10	-9.307869462567911
1110	11	-12.059509225597012
1110	12	-6.814710412540986
1110	13	-5.013696086435385
1110	14	-4.541831628003777
1110	15	-9.965405733965664
1110	16	-11.609168096415413
1110	17	-12.434638033534988
1110	18	-9.035339084621722
1110	19	-12.401160654795987
1110	20	-13.396079899987967
1110	21	-14.083326640373706
1110	22	-14.896461999745837
1110	23	-15.489823845072289
1110	24	-15.881262906662826
1110	25	-16.46595372805344
1111	1	-0.5375354548613309
1111	2	0.1632175877503137
1111	3	-2.588868039278452
1111	4	-3.2989020276638072
1111	5	-1.0749931240070012
1111	6	-0.4824879497171608
1111	7	0.0021433690820096274
1111	8	0.2509893796919158
1111	9	-4.259874231048162
1111	10	-4.423859788717582
1111	11	-1.3331246645257053
1111	12	-5.902981744727054
1111	13	-1.279887801011764
1111	14	0.1067845240035794
1111	15	0.10029278037872658
1111	16	-0.11725010496535049
1111	17	-0.011821117607713205
1111	18	0.26444228796212954
1111	19	0.4641570505218766
1111	20	0.5489583985913278
1111	21	0.6444257306754011
1111	22	0.24642305147322574
1111	23	0.06390070239798362
1111	24	0.21382375616405724
1111	25	0.43602592843780386
1112	1	0.002872143417143036
1112	2	0.49128776492228354
1112	3	-2.452315897446031
1112	4	-0.2492534668155315
1112	5	-0.2859525519031898
1112	6	-0.09204626470623367
1112	7	0.26268219634032874
1112	8	-3.5384952091529307
1112	9	-0.6424966172379953
1112	10	-3.2747769688259467
1112	11	-0.7432289600468067
1112	12	0.2576955043056728
1112	13	0.9051066265484948
1112	14	1.6336738182007302
1112	15	0.8136250400623233
1112	16	0.7612586548308791
1112	17	0.48796477819161765
1112	18	0.5821998947375846
1112	19	0.6698032954483892
1112	20	0.7684195515725847
1112	21	0.6240609334766383
1112	22	0.5921629015311254
1112	23	0.4408952208561949
1112	24	0.4978171206134725
1112	25	0.5201077207873581
1113	1	-0.7848411087569254
1113	2	-0.35417883116472737
1113	3	-2.8016750128965917
1113	4	-1.119018319907866
1113	5	-1.2132908148072694
1113	6	-1.1950670666290506
1113	7	-0.8083681125740583
1113	8	-8.508227487019603
1113	9	-9.872345923803884
1113	10	-3.6136388278588605
1113	11	-9.159696617029681
1113	12	-7.410104050206105
1113	13	-2.570632258806519
1113	14	-0.9437900133941568
1113	15	-6.822194116811602
1113	16	-2.86184763504901
1113	17	-2.1611524441981302
1113	18	-1.3563151922140548
1113	19	-1.093029527613112
1113	20	-6.54083406856947
1113	21	-2.5630664048412903
1113	22	-1.7949650420697125
1113	23	-1.6013326164349522
1113	24	-7.449460261835082
1113	25	-4.64122658686804
1114	1	-3.84585763915619
1114	2	-3.6744791430137056
1114	3	-0.3615258128096137
1114	4	-0.17111146431702906
1114	5	-0.1848146825171213
1114	6	-0.3152600004744741
1114	7	-0.1062221735140092
1114	8	0.1864931037885924
1114	9	-0.060741497437749814
1114	10	-3.1321133223689444
1114	11	-0.8938798818240592
1114	12	-4.418770589800417
1114	13	-0.8995815027166891
1114	14	-5.699435184150893
1114	15	-1.72586833384932
1114	16	-0.6430500003835213
1114	17	-0.5818861249495164
1114	18	-0.17716692728272676
1114	19	0.08167912361072638
1114	20	0.20388744453807917
1114	21	0.36649731402621555
1114	22	0.48838656024375027
1114	23	0.029888222835101175
1114	24	0.08905020793948992
1114	25	0.2072759623114777
1115	1	0.8088587338804842
1115	2	0.8750758480951326
1115	3	1.6813420247709665
1115	4	0.5466974181310569
1115	5	0.2673184077084585
1115	6	-0.2974372948414512
1115	7	-2.331920413386836
1115	8	-0.05045337719663934
1115	9	0.22409912649874641
1115	10	1.0701861719579178
1115	11	0.022084920140034825
1115	12	0.9164256806441884
1115	13	1.0884971271652795
1115	14	1.820892973751949
1115	15	0.8485484817546514
1115	16	0.5831940299162994
1115	17	0.0154061835749566
1115	18	0.4783991208715399
1115	19	0.7092372579916137
1115	20	0.7212306584436021
1115	21	0.9464766468622159
1115	22	0.49842038948384015
1115	23	0.3463961482530067
1115	24	0.18470495736796977
1115	25	0.5386208981050373
1116	1	0.249784201445177
1116	2	0.46037574064128606
1116	3	1.3775943670674806
1116	4	0.713177553884762
1116	5	-0.14350744181401964
1116	6	-1.0495870406209704
1116	7	-4.634977798021104
1116	8	-0.9820020503105376
1116	9	-1.0154818135367911
1116	10	-0.18897081533457083
1116	11	-2.068212596248099
1116	12	-0.3055359891343947
1116	13	-6.28939276424714
1116	14	-1.4225266905358858
1116	15	-1.0075528265201648
1116	16	-0.6152878208181711
1116	17	-0.20766689607633637
1116	18	-0.08956067072217877
1116	19	0.19123205017561062
1116	20	0.3586796111782924
1116	21	0.3632874548529301
1116	22	0.12569550644399285
1116	23	-0.10810126171802636
1116	24	0.26121900144845966
1116	25	0.0752686625266108
1201	1	2.2597623036155703
1201	2	2.4728484157850303
1201	3	3.0562356543608615
1201	4	2.7161321102915004
1201	5	2.187727396946091
1201	6	2.08532574940385
1201	7	2.322238050983664
1201	8	2.3890895134282353
1201	9	2.7870532463265114
1201	10	2.982110674112466
1201	11	2.4830811059725306
1201	12	2.9309790686115953
1201	13	3.0564363271959323
1201	14	3.6416271012112382
1201	15	3.1013105201709337
1201	16	2.614257382775488
1201	17	2.3940277667498293
1201	18	2.687630715397912
1201	19	2.620207819132446
1201	20	2.728560836986233
1201	21	2.6129904434835893
1201	22	1.7540386630022802
1201	23	1.2187109439978698
1201	24	1.7461678957617437
1201	25	1.627457952207461
1202	1	2.0904693149833413
1202	2	2.244643585468637
1202	3	3.0673237249962355
1202	4	2.5458514013073206
1202	5	1.9624011160442052
1202	6	1.7851560877188923
1202	7	2.1066561377084057
1202	8	2.419907440574544
1202	9	2.55020180770941
1202	10	-1.2998102731871946
1202	11	1.2281699043557062
1202	12	2.2439454738443665
1202	13	2.7471893269635785
1202	14	3.456356322038854
1202	15	2.8778636698721627
1202	16	2.6007157937425553
1202	17	2.1619628042989874
1202	18	2.302896321423315
1202	19	2.478983317705449
1202	20	2.5091534253571766
1202	21	2.598749822004507
1202	22	2.5185984413460822
1202	23	2.1232893982790877
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2211	18	0.4799669101259454
2211	19	0.7873113545688817
2211	20	0.9192064562620779
2211	21	0.9944010891849295
2211	22	1.0061888123637992
2211	23	0.8131459963564716
2211	24	0.9291328860778627
2211	25	1.0919152446606013
2212	1	0.9752320370961165
2212	2	1.0691496222549972
2212	3	1.8008673868581795
2212	4	1.2558964577741918
2212	5	0.7259999769543093
2212	6	0.45170214869141034
2212	7	0.7839396813702706
2212	8	1.0418417478557487
2212	9	1.0309757646814575
2212	10	1.755485324542427
2212	11	0.9502066126979791
2212	12	1.1489826308008126
2212	13	1.524837613452494
2212	14	-4.424826263929273
2212	15	-0.8671830445166506
2212	16	-0.20787614290531664
2212	17	0.02220291040599065
2212	18	0.4033293316244091
2212	19	0.806863713266118
2212	20	1.228818330075427
2212	21	1.1854318325761959
2212	22	1.0010478747268792
2212	23	1.0367439224554929
2212	24	1.0023017921681188
2212	25	0.9559268555850942
2213	1	0.7796461124620677
2213	2	0.871988309173318
2213	3	1.6016781188657596
2213	4	1.043725668604111
2213	5	0.44935965975282244
2213	6	0.17408038590498975
2213	7	0.45174140884408587
2213	8	-5.325889606387975
2213	9	-1.756405469243841
2213	10	0.2520923809436013
2213	11	0.05603715180011548
2213	12	0.621202033294292
2213	13	-8.166246494256345
2213	14	-2.351405656991659
2213	15	-0.7793675123708432
2213	16	-0.23476177650736574
2213	17	-0.07620649260944035
2213	18	0.26338757402149326
2213	19	0.6584431513241782
2213	20	0.8158394186467071
2213	21	0.7369970520020885
2213	22	0.5890511288138356
2213	23	0.48011525013376755
2213	24	0.5755112794154478
2213	25	0.5562051088171316
2214	1	-0.9210960916597521
2214	2	-0.5989813771200261
2214	3	-7.093980117063502
2214	4	-2.642442457615367
2214	5	-1.714088000525166
2214	6	-1.076400165858793
2214	7	-0.7444542889406698
2214	8	-6.2715878603675925
2214	9	-2.0278270578625737
2214	10	-0.3079165959698038
2214	11	-0.6348309838306427
2214	12	-0.2376158938270052
2214	13	-6.39937275261288
2214	14	-1.7650265947573587
2214	15	-0.9148221107196584
2214	16	-0.9128713264899773
2214	17	-0.7951674336516561
2214	18	-0.6938484804588256
2214	19	-0.2783328872883466
2214	20	0.01851066707849469
2214	21	-0.05179505174615073
2214	22	-0.2375576973582092
2214	23	-0.2688947555853929
2214	24	-0.032409961843928414
2214	25	0.05158567176183837
2215	1	0.3110191159134139
2215	2	0.6530374792803002
2215	3	-2.3532198780562688
2215	4	-0.5344033412987592
2215	5	-0.07331902322111716
2215	6	-0.5430421892711408
2215	7	0.21375437997065205
2215	8	0.4888871617094033
2215	9	0.6893196628108988
2215	10	1.4379456229127676
2215	11	0.5469783968252848
2215	12	0.29490983408075166
2215	13	1.0886954308891994
2215	14	1.0460247552645967
2215	15	0.6572624167203465
2215	16	0.33434892070952316
2215	17	0.38328753668348625
2215	18	0.4727482708525699
2215	19	0.4116063873863176
2215	20	0.6331710863568851
2215	21	0.8016915511336435
2215	22	0.5412824764988713
2215	23	0.33650917600155594
2215	24	0.43707564524722287
2215	25	0.38395572686699353
2216	1	0.36762451327220447
2216	2	0.06558838634858688
2216	3	1.2377259917351857
2216	4	-0.12942023517956613
2216	5	-0.25425134348391865
2216	6	-0.49095772577570074
2216	7	-1.0418258841813604
2216	8	-6.504390737556189
2216	9	-2.206064925698488
2216	10	-0.7341568541000036
2216	11	-1.2844308885965674
2216	12	0.08857328221700556
2216	13	0.5221830699735293
2216	14	1.2604029091393905
2216	15	0.7501132956071856
2216	16	0.012596040032462952
2216	17	-0.017178745901468062
2216	18	0.1460312258229699
2216	19	0.3240106499003659
2216	20	0.41512651891464714
2216	21	0.08090303929799347
2216	22	-0.032007817121634474
2216	23	-0.40477377931137326
2216	24	-0.2339571614302116
2216	25	-0.12440977764122252
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	177182.0
3	4.0
4	1207.0
5	8000.0
6	11616.0
7	10264.0
8	8919.0
9	7508.0
10	6794.0
11	6199.0
12	5794.0
13	5823.0
14	5788.0
15	5974.0
16	6346.0
17	6779.0
18	7666.0
19	10036.0
20	14061.0
21	17718.0
22	21753.0
23	25652.0
24	29795.0
25	33846.0
26	37838.0
27	41885.0
28	46003.0
29	53900.0
30	60713.0
31	68487.0
32	85857.0
33	106676.0
34	133079.0
35	164128.0
36	203145.0
37	235842.0
38	311036.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	46.8697707263872	17.030359379457845	11.77098740826324	24.32888248589171
2	14.595292679817648	20.829396065227463	41.04063260685257	23.53467864810232
3	16.10635882367226	27.79071249908934	30.378247002922205	25.724681674316198
4	10.745306482588907	16.165491896004973	37.63642416405101	35.45277745735512
5	12.619848718662249	39.249462419995446	33.83455184801032	14.296137013331983
6	30.93444929091319	36.5177936644866	18.991572672928083	13.556184371672126
7	28.865692639981148	32.238611083999565	21.554322865016005	17.34137341100329
8	27.027811547385223	34.01028191834745	21.210932047908116	17.750974486359215
9	28.384899118774758	15.333248376464114	19.376993072980987	36.90485943178014
10	13.277207712387328	30.661063217517913	33.79171450963238	22.27001456046237
11	34.867170444494676	23.52984309662046	22.842142365530712	18.760844093354148
12	22.80942888437814	26.802151000911252	30.12963856347769	20.25878155123292
13	27.810263712525433	22.09735195245974	26.836359885275545	23.256024449739282
14	21.059106948350824	21.177486708193133	29.336739595880523	28.426666747575513
15	23.674249319401802	29.104977974102887	23.920043364117003	23.30072934237831
16	23.181157818992364	28.82118041245715	25.2833917301244	22.714270038426083
17	22.648824993982885	28.243932826749564	26.28776371649377	22.81947846277378
18	21.931734922810797	27.60759741372815	28.363232951049035	22.09743471241201
19	23.454164467070786	26.667344243328905	27.634407949693475	22.24408333990684
20	23.416308734556736	26.80691120547887	27.710328811179032	22.066451248785363
21	24.421110010462066	25.92867895314623	27.310237907061058	22.339973129330644
22	23.815897592783152	26.57551217514203	26.83375514197801	22.774835090096808
23	23.288561996158123	26.56973148827489	27.010045614075896	23.13166090149109
24	22.908479149516285	26.713993296939126	27.367410260092516	23.010117293452076
25	22.995957558152764	26.345799425108645	27.706679003825023	22.95156401291357
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	6772.0
1	6772.0
2	4814.5
3	2857.0
4	2857.0
5	2857.0
6	5358.0
7	7859.0
8	7859.0
9	7859.0
10	8108.5
11	8358.0
12	8358.0
13	8358.0
14	10315.0
15	12272.0
16	12272.0
17	12272.0
18	18593.0
19	24914.0
20	24914.0
21	24914.0
22	36198.5
23	47483.0
24	47483.0
25	47483.0
26	66913.0
27	86343.0
28	86343.0
29	86343.0
30	105660.5
31	124978.0
32	124978.0
33	124978.0
34	144740.5
35	164503.0
36	164503.0
37	164503.0
38	182472.5
39	200442.0
40	200442.0
41	200442.0
42	218793.5
43	237145.0
44	237145.0
45	237145.0
46	247236.0
47	257327.0
48	257327.0
49	257327.0
50	251583.5
51	245840.0
52	245840.0
53	245840.0
54	226898.0
55	207956.0
56	207956.0
57	207956.0
58	180164.0
59	152372.0
60	152372.0
61	152372.0
62	125314.5
63	98257.0
64	98257.0
65	98257.0
66	74898.5
67	51540.0
68	51540.0
69	51540.0
70	38446.0
71	25352.0
72	25352.0
73	25352.0
74	18324.5
75	11297.0
76	11297.0
77	11297.0
78	8431.0
79	5565.0
80	5565.0
81	5565.0
82	3912.5
83	2260.0
84	2260.0
85	2260.0
86	1643.5
87	1027.0
88	1027.0
89	1027.0
90	760.0
91	493.0
92	493.0
93	493.0
94	283.5
95	74.0
96	74.0
97	74.0
98	50.5
99	27.0
100	27.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.3257680456892079
2	0.3265747766489707
3	0.33887742378535307
4	0.3406925684448193
5	0.3460371610532478
6	0.28734748373050545
7	0.296776151822733
8	0.29551563469810366
9	0.2944063796284298
10	0.4084579690648929
11	0.29516268990320743
12	0.29319628318878566
13	0.2876500078404165
14	0.29138113852931935
15	0.2844230840013654
16	0.2899189386647493
17	0.28341467030166195
18	0.2843726633163802
19	0.28623822866083165
20	0.4777359902345217
21	0.2869945389356093
22	0.2883054767452238
23	0.2840701392064692
24	0.2878516905803572
25	0.2797339602977442
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1983313.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	63.069967104383764
#Duplication Level	Percentage of deduplicated	Percentage of total
1	85.65028686169377	54.01960774848059
2	7.868567481622053	9.925405844490532
3	2.5637663717777084	4.85089982194036
4	1.1941588630157014	3.0126224083123434
5	0.6591718299186275	2.0786972814552143
6	0.4125575302125937	1.5611993915504454
7	0.2853313190460918	1.2597085834261206
8	0.20693983273252733	1.0441350754421739
9	0.1477932611159018	0.8389184505146573
>10	0.8821235987085335	10.477327745653767
>50	0.08020684156190266	3.542912032258267
>100	0.04639224440404587	5.3709350090363985
>500	0.001966519411295736	0.8184835193028404
>1k	6.555064704319111E-4	0.9284036130998553
>5k	8.193830880398889E-5	0.2707434750364677
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
NNNNNNNNNNNNNNNNNNNNNNNNN	5239	0.26415396863732554	No Hit
GTATCAACGCAGAGTACTTTTTTTT	4627	0.23329650942639918	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	3299	0.16633783976608837	No Hit
TATCAACGCAGAGTACTTTTTTTTT	3077	0.15514444769937977	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	5.0420684985173795E-5	0.0
5	0.0	0.0	0.0	1.0084136997034759E-4	0.0
6	0.0	0.0	0.0	1.0084136997034759E-4	0.0
7	0.0	0.0	0.0	1.0084136997034759E-4	0.0
8	0.0	0.0	0.0	1.0084136997034759E-4	0.0
9	0.0	0.0	0.0	1.0084136997034759E-4	0.0
10	0.0	0.0	0.0	1.0084136997034759E-4	0.0
11	0.0	0.0	0.0	1.0084136997034759E-4	0.0
12	0.0	0.0	0.0	1.0084136997034759E-4	0.0
13	0.0	0.0	0.0	1.0084136997034759E-4	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAACGCA	195	0.0	15.108323	4
ATAACGC	210	0.0	13.5608015	3
TATAACG	210	0.0	13.108775	2
TGCGTAG	60	4.0588796E-4	12.679205	14
ACGGGTT	60	4.0797566E-4	12.671496	5
GGACCGA	100	1.4441139E-7	12.349391	6
TATCACG	70	1.0991968E-4	12.204722	2
CGAAATC	55	0.0030969665	12.076247	13
TCGTCGC	55	0.0030969665	12.076247	13
GTATAAC	390	0.0	11.926552	1
ACGAAAT	65	8.1106543E-4	11.677539	12
GTTATCA	375	0.0	11.644208	1
AGCGTTC	90	7.5690605E-6	11.596444	12
GACGTCT	115	6.98019E-8	11.576665	15
GTATTAG	175	1.8189894E-12	11.391074	1
GGTATCA	1670	0.0	11.368337	1
TCCCGAC	135	4.78758E-9	11.2504425	2
CGACCCG	110	4.9457594E-7	11.231553	5
CGCATAT	110	4.9749906E-7	11.226435	8
CGGTTTG	85	5.2709296E-5	11.187533	14
>>END_MODULE