FastQCFastQC Report
Thu 26 May 2016
SRR1364303_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364303_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1983313
Sequences flagged as poor quality0
Sequence length25
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT100690.507685877115715No Hit
TATCAACGCAGAGTACTTTTTTTTT79810.4024074868666721No Hit
GTACTTTTTTTTTTTTTTTTTTTTT73090.3685247865566353No Hit
GGTATCAACGCAGAGTACTTTTTTT65220.32884370747330355No Hit
GAGTACTTTTTTTTTTTTTTTTTTT40780.20561555336953874No Hit
ACGCAGAGTACTTTTTTTTTTTTTT33090.1668420466159401No Hit
GTGGTATCAACGCAGAGTACATGGG22090.11137929313224891No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAACGCA1600.014.8450914
TATAACG1650.014.394152
GTGCGAG551.9615969E-413.81733811
ATAACGC1850.013.3518843
GCCGACC500.001502775213.29650513
TATCACG1153.783498E-1013.2175852
CGAAATC802.0061052E-613.05906813
AGGACCG1800.012.6710065
GGTATCA28850.012.4181521
TACAACG1251.4115358E-912.1601772
CCGAGTG1103.8096914E-812.0923069
GTATAAC3000.012.0371521
AAGTGGC2750.011.4018927
CGCGCCA1001.9270065E-611.40160510
TGGTCGG1001.9270065E-611.40160510
ACCGAGT1354.7202775E-911.2608458
CTACGCT600.00587267911.0843334
CGTCTTA600.005888134211.080420515
ATCGTAG600.005888134211.080420513
TTTATAC2500.011.0204383
Produced by FastQC (version 0.11.5)