##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1364303_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1983313
Sequences flagged as poor quality	0
Sequence length	25
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.822516163611088	31.0	30.0	34.0	26.0	34.0
2	29.828077060958105	31.0	30.0	34.0	26.0	34.0
3	29.887212961342964	31.0	30.0	34.0	26.0	34.0
4	33.27588585361968	35.0	33.0	37.0	28.0	37.0
5	32.85518876748148	35.0	33.0	37.0	26.0	37.0
6	32.757639364033814	35.0	33.0	37.0	26.0	37.0
7	32.485819434451344	35.0	33.0	37.0	25.0	37.0
8	32.3769818480492	35.0	33.0	37.0	23.0	37.0
9	33.15905305919943	37.0	32.0	39.0	17.0	39.0
10	33.27557324537277	37.0	32.0	39.0	19.0	39.0
11	33.24777178387879	37.0	33.0	39.0	18.0	39.0
12	33.1113777805117	37.0	32.0	39.0	17.0	39.0
13	33.22351691336667	37.0	33.0	39.0	18.0	39.0
14	34.16394840350464	38.0	33.0	41.0	18.0	41.0
15	34.20275014584183	38.0	33.0	41.0	18.0	41.0
16	34.14168061218779	38.0	33.0	41.0	18.0	41.0
17	34.089382260893764	38.0	33.0	41.0	17.0	41.0
18	34.03328975305461	38.0	33.0	41.0	17.0	41.0
19	33.934766221973035	39.0	33.0	41.0	18.0	41.0
20	33.77081378481359	39.0	33.0	41.0	16.0	41.0
21	33.6908617046326	39.0	33.0	41.0	11.0	41.0
22	33.672728409484534	39.0	33.0	41.0	11.0	41.0
23	33.363262883871585	38.0	32.0	41.0	8.0	41.0
24	33.2725686767545	38.0	32.0	41.0	2.0	41.0
25	33.136837201188115	38.0	32.0	41.0	2.0	41.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1101	1	0.2573211241750748
1101	2	0.3565405130763004
1101	3	-1.5585615656459133
1101	4	-0.512368249766439
1101	5	0.29605969850550196
1101	6	0.6913797348422008
1101	7	0.62539990985929
1101	8	0.8493915668452345
1101	9	1.6295842810338428
1101	10	1.2692851704068886
1101	11	1.2615909119263407
1101	12	1.2524821958804893
1101	13	1.113529365984597
1101	14	1.2578227111464386
1101	15	1.2463952314863889
1101	16	1.2047865642963984
1101	17	1.0989842042858697
1101	18	1.1021768243285237
1101	19	1.1541191664857422
1101	20	1.3692183083026919
1101	21	1.4147982086483708
1101	22	1.4114464973536016
1101	23	1.6541622857510418
1101	24	1.5961667266947401
1101	25	1.7580567318713491
1102	1	0.6669918018754153
1102	2	0.603676821592753
1102	3	0.8332897729199722
1102	4	0.7098527879497141
1102	5	0.9394934631471088
1102	6	0.9755388169684522
1102	7	1.136764505269035
1102	8	1.3342662387000388
1102	9	1.7948697395931603
1102	10	1.412376686826363
1102	11	1.3613195119734272
1102	12	1.5671513411260989
1102	13	1.277608405113277
1102	14	1.4233198261338487
1102	15	1.439579583568971
1102	16	1.412619628894916
1102	17	1.3182320577387827
1102	18	1.4588054683503913
1102	19	1.3335862720119067
1102	20	1.5472977649697555
1102	21	1.665800522877099
1102	22	1.7254451381328906
1102	23	2.063655065446234
1102	24	2.020928529927552
1102	25	1.8440065650780895
1103	1	-3.0776289484092203
1103	2	-2.915446357100663
1103	3	-2.960090831516016
1103	4	-3.1044092496077553
1103	5	-5.696984551466581
1103	6	-11.344046984406447
1103	7	-11.551188569514427
1103	8	-12.395903677854935
1103	9	-12.978861651013151
1103	10	-11.078636038900356
1103	11	-13.670370862304178
1103	12	-12.074239697541085
1103	13	-12.139329926274446
1103	14	-12.91018789145014
1103	15	-12.853151043205493
1103	16	-7.940739069420328
1103	17	-6.2391043309219185
1103	18	-5.18378031861258
1103	19	-5.20329383521279
1103	20	-9.839335177800503
1103	21	-6.77290125123044
1103	22	-12.888641073981773
1103	23	-13.374692175391385
1103	24	-13.661816076410812
1103	25	-14.448914299060725
1104	1	0.42458158963117043
1104	2	0.5687300175598331
1104	3	0.5875257001098149
1104	4	0.7115348986068213
1104	5	0.6711300080965685
1104	6	0.7920324807233143
1104	7	0.7339979696226635
1104	8	0.9644354426707977
1104	9	1.3897841231178418
1104	10	1.08442684148212
1104	11	0.9960763689540855
1104	12	0.9309653484261133
1104	13	0.890145342260233
1104	14	1.0681294150064033
1104	15	1.000742102698581
1104	16	0.6697464207107942
1104	17	0.600808732672462
1104	18	0.7126856765150933
1104	19	1.0660490866386922
1104	20	1.3565773260193907
1104	21	1.1292396005978205
1104	22	0.889535432054366
1104	23	1.283961052879512
1104	24	1.4065478112801486
1104	25	1.3770143982355094
1105	1	0.035007854958070794
1105	2	0.1528528225809005
1105	3	-0.14720859208395964
1105	4	0.005724449968177225
1105	5	0.07303526118771231
1105	6	0.19312396353301153
1105	7	0.42591935640746215
1105	8	0.21196203191691865
1105	9	0.4405874367732494
1105	10	0.049301810724173833
1105	11	0.5542819861591255
1105	12	0.6258781461769303
1105	13	0.4643897490388724
1105	14	0.3473565436598349
1105	15	0.2194848426437943
1105	16	0.43059050017771483
1105	17	0.3172226724929672
1105	18	0.3062617438531916
1105	19	0.33951841479939304
1105	20	0.6069126036696488
1105	21	0.6410742682424484
1105	22	0.3541456575610624
1105	23	0.692009949522955
1105	24	0.685589211360103
1105	25	0.829737350894689
1106	1	-0.18356159119476345
1106	2	0.03679811525246279
1106	3	-0.11008910322621546
1106	4	-1.41773522810783
1106	5	-0.33867406996504457
1106	6	0.0180340493412956
1106	7	0.08402562320141271
1106	8	0.359309996373959
1106	9	0.9062589260052007
1106	10	0.4357966211680804
1106	11	0.5457589783475001
1106	12	0.6786652695638011
1106	13	0.4218587338400823
1106	14	0.048056626990714335
1106	15	0.40365409788056184
1106	16	0.18013031795855028
1106	17	0.20885398272811528
1106	18	0.4182939171952782
1106	19	-0.9547931266139713
1106	20	0.16296344763301107
1106	21	0.05286697358549475
1106	22	0.49774979603931513
1106	23	0.4180815569080494
1106	24	0.4717677629273709
1106	25	0.8389165244117578
1107	1	0.21992838816152016
1107	2	0.08077398892559629
1107	3	-0.013886724528408223
1107	4	0.17096087992162268
1107	5	0.2475814690896314
1107	6	0.26580053882105403
1107	7	0.04317726411556322
1107	8	0.016582086393938766
1107	9	0.7493791008897972
1107	10	0.5064742518280738
1107	11	0.6272829157188013
1107	12	0.5292171612189875
1107	13	0.3898173752795344
1107	14	0.44977273774524207
1107	15	0.5341918671422476
1107	16	0.2582721220429036
1107	17	0.032249295773077336
1107	18	0.2557005427230479
1107	19	0.3907557271841213
1107	20	0.34385819723902955
1107	21	0.30491849118578784
1107	22	0.3353441988411703
1107	23	0.4799322631562646
1107	24	0.6502372613852998
1107	25	0.438969676199207
1108	1	0.03443579026503585
1108	2	0.09636143414357079
1108	3	0.14555956756489863
1108	4	-0.010089268817701225
1108	5	0.17198807579572417
1108	6	0.26528508168382814
1108	7	-0.1464640151463925
1108	8	0.28792588849774603
1108	9	0.5560031100918792
1108	10	0.4573330066269321
1108	11	0.3864336434063276
1108	12	0.3814041592297741
1108	13	-0.10738188515710334
1108	14	0.03254173284872053
1108	15	-0.21805665420692577
1108	16	-0.701037456273589
1108	17	-0.8089395366020824
1108	18	-0.6149214268942842
1108	19	-0.4146133256890536
1108	20	0.06588328502983387
1108	21	-0.23721259041973752
1108	22	0.007531746530311523
1108	23	-0.3645063018923409
1108	24	-0.09306358709778095
1108	25	-0.2231700933187355
1109	1	0.06282216300951049
1109	2	0.17457177852360317
1109	3	-0.039781309404396836
1109	4	0.11847295832409799
1109	5	0.04827215081414238
1109	6	0.27375137597651644
1109	7	0.34200001441072914
1109	8	0.13265538597211446
1109	9	1.108682936474807
1109	10	0.39282167657029277
1109	11	0.45409936632773906
1109	12	0.539173673042292
1109	13	0.5759002227676646
1109	14	0.09603582972268043
1109	15	0.0402640612386449
1109	16	0.3901402916240144
1109	17	0.21880969475320455
1109	18	0.13903519161090117
1109	19	0.38064530068134417
1109	20	0.7330080651063966
1109	21	0.7012551346206948
1109	22	0.27737889058594334
1109	23	0.7182763200789068
1109	24	0.42548241471084225
1109	25	0.479410134253456
1110	1	0.07837945153397285
1110	2	0.09399185878008254
1110	3	0.06008309639504361
1110	4	0.16880124673622987
1110	5	0.2627737640221568
1110	6	0.3910487007639034
1110	7	0.28645067691746107
1110	8	0.22119017896271487
1110	9	-3.4573743701983055
1110	10	-1.4199147551375368
1110	11	-0.35052082583364097
1110	12	0.02248788204259
1110	13	-0.34170347555379266
1110	14	0.1660844751911128
1110	15	0.20641381988177443
1110	16	-0.21452592430896544
1110	17	0.028541134115315003
1110	18	0.03476849881826638
1110	19	-0.052613304687156415
1110	20	0.2546981324141413
1110	21	0.04363610245653149
1110	22	0.18439651479881292
1110	23	0.4672063331085212
1110	24	0.4324226821845798
1110	25	0.4997815173759719
1111	1	0.04960099781878213
1111	2	0.0935294835625946
1111	3	0.08470117396499077
1111	4	-0.07285055369982274
1111	5	0.2822042318467979
1111	6	-0.018804595185756057
1111	7	0.07003925905186037
1111	8	0.3311892432033403
1111	9	0.8702265779711311
1111	10	0.4564583216142495
1111	11	0.4698072704164673
1111	12	0.45063452304175655
1111	13	0.21657426683415792
1111	14	0.154262381562134
1111	15	0.19429288374209364
1111	16	0.20066260117946655
1111	17	0.1481384873674827
1111	18	0.005259051902093859
1111	19	0.19067152094884676
1111	20	0.28553765868971936
1111	21	0.31590905195504604
1111	22	0.37543964630003046
1111	23	0.767084584309373
1111	24	0.6917794502018282
1111	25	0.89961268036474
1112	1	-0.19402191795043322
1112	2	-0.29410533193432187
1112	3	-0.13301414550179302
1112	4	-0.15622294680580495
1112	5	-0.10880843063329593
1112	6	-0.14034227238736463
1112	7	-0.13271238007536112
1112	8	-0.4176016164146503
1112	9	0.1365621257893821
1112	10	0.0020968994960099963
1112	11	0.1448672433051641
1112	12	-0.6423840201406961
1112	13	-0.4777959881938827
1112	14	-1.3095429426866758
1112	15	-0.6269479918818419
1112	16	-0.6936071720894219
1112	17	-0.5107750449665005
1112	18	-0.7653095740765394
1112	19	-0.4318763091589304
1112	20	-0.33887644472054035
1112	21	-0.7503130710259285
1112	22	-0.3578256453045583
1112	23	-0.260758940792293
1112	24	-0.2075423720254861
1112	25	-0.29861727960064144
1113	1	-2.233999753590812
1113	2	-2.248696182697966
1113	3	-2.3215842581724573
1113	4	-2.3384692287179725
1113	5	-2.102733866381964
1113	6	-2.2632069993607544
1113	7	-2.657393065827879
1113	8	-2.7267581161626318
1113	9	-11.412821806260457
1113	10	-4.966497996758498
1113	11	-3.4044424040239996
1113	12	-12.96555309436599
1113	13	-5.454162441874335
1113	14	-4.361833058086614
1113	15	-3.858849482638618
1113	16	-3.407018645612144
1113	17	-3.6242606381327462
1113	18	-3.5983653943669474
1113	19	-4.228222821518806
1113	20	-14.342715314008043
1113	21	-17.1513454635662
1113	22	-11.708266291262479
1113	23	-18.125342572181605
1113	24	-19.66566449830731
1113	25	-15.97973250785833
1114	1	-0.1386398695881219
1114	2	-0.083365359509461
1114	3	-0.03871281049226383
1114	4	-0.4779874083561779
1114	5	-0.11967482860290346
1114	6	0.07152198716640612
1114	7	-0.10385442679516643
1114	8	0.03049520928551175
1114	9	0.7584064225838105
1114	10	0.10857409630765602
1114	11	0.06369746351145267
1114	12	-0.10477557924168224
1114	13	0.01272170111672466
1114	14	-0.04820432438126687
1114	15	0.016125293871482427
1114	16	-0.12287302740047323
1114	17	-0.07461046907248203
1114	18	-0.034603486224604296
1114	19	-0.20392023354681754
1114	20	-0.604477285872882
1114	21	-0.7590029517705545
1114	22	-0.8071152616689687
1114	23	-0.5393367600187702
1114	24	-0.24080310872079735
1114	25	-0.4940910318887006
1115	1	-0.1951430770929079
1115	2	-0.14950723346755623
1115	3	-0.2368563342174319
1115	4	-0.07923791827393245
1115	5	-0.3900074940660545
1115	6	-0.42434608255352657
1115	7	-0.7163297009487373
1115	8	-0.37377435183588403
1115	9	0.12054419315951037
1115	10	-0.3558379638201927
1115	11	-0.19600581130623596
1115	12	-0.4050166585920181
1115	13	-0.5765563529995461
1115	14	-0.7422974295418996
1115	15	-0.7120688197055145
1115	16	-0.5563003058031768
1115	17	-0.34798066673651107
1115	18	-0.4522576842282078
1115	19	-0.48688928088146355
1115	20	-0.835733723232444
1115	21	-0.3261577120923036
1115	22	-0.8551181495125988
1115	23	-0.5294019092903852
1115	24	0.002695674613548249
1115	25	-0.005057362666995857
1116	1	-0.563888940554623
1116	2	-0.3965085253885192
1116	3	-0.47921333247937525
1116	4	-0.21442534045517903
1116	5	-1.214417944345488
1116	6	-0.9343644062267202
1116	7	-2.0912476390522485
1116	8	-1.3186528366864962
1116	9	-0.2603246746889809
1116	10	-1.3136202743019467
1116	11	-0.9562757970359073
1116	12	-1.8940849939536974
1116	13	-1.6596995602476774
1116	14	-1.071827655910667
1116	15	-2.283112767425358
1116	16	-2.4186778651059626
1116	17	-3.0110525792109577
1116	18	-2.331952770146138
1116	19	-1.8360494820907078
1116	20	-2.330902564225834
1116	21	-1.555838551095139
1116	22	-1.4536452659737762
1116	23	-1.5233029086144896
1116	24	-2.0479557873172567
1116	25	-1.5319255897387443
1201	1	0.5544274498816684
1201	2	0.6825835817169477
1201	3	0.22912349491936013
1201	4	0.6087623020505148
1201	5	0.7953867509954051
1201	6	0.9595493176147443
1201	7	1.1502989677327804
1201	8	0.9711950661722923
1201	9	1.736582935138827
1201	10	1.5260146455078285
1201	11	1.438912580836167
1201	12	1.4984445108199225
1201	13	1.3406491506413971
1201	14	1.5071497636363418
1201	15	1.466785541042242
1201	16	1.3851364970016462
1201	17	1.2981766672737578
1201	18	1.3730381971414403
1201	19	1.5215485069971848
1201	20	1.7144403809810242
1201	21	1.7152692719054343
1201	22	1.7792123116200926
1201	23	1.9553062965182022
1201	24	1.9632595934511272
1201	25	2.0945304869184582
1202	1	0.677233575238418
1202	2	0.6513886286040744
1202	3	0.6269395939281317
1202	4	0.8215995978098505
1202	5	0.8951679882543431
1202	6	0.8334902472906336
1202	7	0.9878766197378113
1202	8	0.8996256736663355
1202	9	1.6367721057255409
1202	10	1.2655313508269685
1202	11	1.4145472777467418
1202	12	1.3665665269443608
1202	13	1.3324422598342522
1202	14	1.3835933127658961
1202	15	1.4016247131633648
1202	16	1.1861858128748821
1202	17	1.2844579637170028
1202	18	1.2895479888565191
1202	19	1.3748479226069321
1202	20	1.506244669432192
1202	21	1.5595169955015606
1202	22	1.6946811171053753
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2211	7	0.29132660782075703
2211	8	0.32773701870486605
2211	9	0.8212421939117647
2211	10	0.22187519765305552
2211	11	0.6651480960513467
2211	12	0.5567180721894545
2211	13	0.4306189500702118
2211	14	0.4965660227047195
2211	15	0.48233596286544156
2211	16	0.5420559566740408
2211	17	0.4346069269330144
2211	18	0.3250012342684485
2211	19	0.12206429664363583
2211	20	0.6388507312419947
2211	21	0.8496983333898598
2211	22	0.5325992071983308
2211	23	0.9586673410640429
2211	24	1.0450350259430081
2211	25	1.1497610124740518
2212	1	0.1325389254500493
2212	2	0.17154603366365961
2212	3	0.20265006224231996
2212	4	0.2370192471106023
2212	5	-0.059193321699041235
2212	6	0.19950869898659107
2212	7	0.11994863206494699
2212	8	-0.06047497194796847
2212	9	0.5701577773789737
2212	10	0.15951211893244732
2212	11	0.3065804751954957
2212	12	0.1168776311203672
2212	13	-0.035899233075689096
2212	14	0.03119615268865772
2212	15	0.24630375898052392
2212	16	0.33157874037492974
2212	17	0.08699848527973586
2212	18	0.09928194114737465
2212	19	0.2519149611682465
2212	20	0.5030595088088106
2212	21	0.581294287822459
2212	22	0.27194125481042164
2212	23	0.6041562413123245
2212	24	0.7399123444766715
2212	25	0.7652294658112808
2213	1	-0.008200965372456182
2213	2	-0.064574241565861
2213	3	-0.1624197980371207
2213	4	0.021467033233577126
2213	5	0.022003797138125947
2213	6	0.3060357710822572
2213	7	0.13367249182964258
2213	8	0.22530981555546958
2213	9	0.8071041731356985
2213	10	0.12198662888459211
2213	11	0.4455511362179081
2213	12	0.33116375679507826
2213	13	-0.021760693203226822
2213	14	0.12800694949000757
2213	15	0.11570769232213962
2213	16	-0.019234061433536453
2213	17	0.02962459464801981
2213	18	-0.2554737413559707
2213	19	-0.033531548877483885
2213	20	0.3826464125486737
2213	21	-0.05543675865087039
2213	22	0.13503841486362944
2213	23	-0.23218604255996667
2213	24	0.08518050967917645
2213	25	0.3192692671823849
2214	1	-0.9494721638788768
2214	2	-0.672934407772825
2214	3	-0.7535586621465491
2214	4	-0.5900291538573157
2214	5	-0.8124421318396884
2214	6	-0.7554206928986815
2214	7	-0.7242418451429913
2214	8	-0.7004378650734076
2214	9	-10.617297908929054
2214	10	-3.7904069546064676
2214	11	-1.7349615077431757
2214	12	-1.070120896356343
2214	13	-0.8256907472818895
2214	14	-0.8311762479160194
2214	15	-0.9012444096242831
2214	16	-1.073884641514283
2214	17	-0.9040738749062243
2214	18	-1.2484099186655584
2214	19	-1.171913296678234
2214	20	-1.0421238087665614
2214	21	-1.536702546341509
2214	22	-1.399438643733255
2214	23	-1.6196881306039685
2214	24	-1.8905290029800668
2214	25	-9.68099939671345
2215	1	-0.5952683111790797
2215	2	-0.3613069857580804
2215	3	-0.21175031248391463
2215	4	-0.5527520107361354
2215	5	-0.4224082087476546
2215	6	-0.40869585234565875
2215	7	-0.3833360097658556
2215	8	-0.6457879461204357
2215	9	0.2925586114703762
2215	10	0.0948890244388636
2215	11	0.1983702270117078
2215	12	0.1026353214178215
2215	13	0.021209442957925262
2215	14	-0.1650588456203721
2215	15	-0.7081441256790271
2215	16	-0.2885330445119578
2215	17	-0.13544430657539408
2215	18	-0.16872662040665176
2215	19	-0.2491592118388013
2215	20	-0.030890651878571873
2215	21	-8.380346663585669E-4
2215	22	-0.22783331263577367
2215	23	0.3281575289816274
2215	24	0.4510734713319877
2215	25	0.5600026497363899
2216	1	-0.5428622144306665
2216	2	-0.4884397220718206
2216	3	-0.5775979475504016
2216	4	-0.36583359554545325
2216	5	-1.188344404280123
2216	6	-1.8920206324837245
2216	7	-1.0616239754843413
2216	8	-1.9065136572651724
2216	9	-0.5950068465911187
2216	10	-1.0835699052811023
2216	11	-0.6870828644139166
2216	12	-1.8230124214934271
2216	13	-1.6255107670250872
2216	14	-1.8081255724462721
2216	15	-1.3619880498033794
2216	16	-2.3802741345386984
2216	17	-2.6833640219145494
2216	18	-2.261214163326411
2216	19	-3.538836368607697
2216	20	-2.3681998437647493
2216	21	-2.676986145923035
2216	22	-2.457291150806469
2216	23	-1.9094527188224077
2216	24	-2.124174452159714
2216	25	-2.241647339127187
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	62386.0
3	3.0
4	900.0
5	9225.0
6	12397.0
7	9824.0
8	7876.0
9	6377.0
10	5678.0
11	5364.0
12	4775.0
13	4493.0
14	4500.0
15	4483.0
16	4825.0
17	5035.0
18	5690.0
19	6896.0
20	9228.0
21	12240.0
22	15227.0
23	17955.0
24	20776.0
25	24096.0
26	27083.0
27	30100.0
28	33508.0
29	39492.0
30	47361.0
31	59061.0
32	73374.0
33	90814.0
34	113017.0
35	141385.0
36	182488.0
37	264596.0
38	620785.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.88661839313391	18.118008058589954	11.700136528996147	25.29523701927999
2	16.418543348217707	21.27618379176398	38.15033419251264	24.154938667505675
3	18.717314708557623	26.714963081881383	29.19627208683674	25.371450122724255
4	12.108001375531078	16.547467419376634	36.28457358032385	35.05995762476844
5	12.988304055222976	38.93510362125899	33.792650554585194	14.28394176893284
6	31.49979098202143	36.5868196137025	18.37860543406403	13.53478397021204
7	28.659358548476	31.972117732079486	21.643504568288307	17.725019151156204
8	27.374476364931148	34.51210274090744	20.023411644494026	18.090009249667386
9	27.70385867788937	14.673255161783635	19.073229337734183	38.549656822592816
10	14.931841406811925	30.49815846204596	32.40864690590352	22.161353225238603
11	35.424795919602616	22.604804312825085	22.592850023605937	19.377549743966362
12	23.16776155889525	26.627563853025592	29.66329290602618	20.54138168205298
13	28.896886907424058	21.3838869059114	26.079568631385857	23.639657555278685
14	22.059308487840298	20.759029644159156	28.279589953559668	28.902071914440874
15	24.167121323206846	28.766598325317595	23.708010661653848	23.35826968982171
16	23.365406821658834	28.155705507489586	25.46882390374121	23.01006376711037
17	22.711624757137866	27.612838550950748	26.90244316912584	22.773093522785548
18	22.004480017427895	26.772743501352476	28.956726783484648	22.266049697734978
19	23.63093833271809	25.89436822857097	27.98099209062797	22.49370134808297
20	23.26547161048168	26.08321823012875	28.32048161298798	22.330828546401595
21	24.262856053649525	25.199927587864007	27.918357837983393	22.618858520503075
22	23.66312978354114	25.816523809667885	27.706290447995112	22.81405595879587
23	22.901107478848076	25.522488683424825	28.253493300819454	23.322910536907642
24	22.90939729972852	25.885736644870335	28.080816535633176	23.124049519767972
25	22.914437315005877	25.501831265168	28.445181168052397	23.138550251773722
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	663.0
1	663.0
2	1177.0
3	1691.0
4	1691.0
5	1691.0
6	5379.5
7	9068.0
8	9068.0
9	9068.0
10	8566.5
11	8065.0
12	8065.0
13	8065.0
14	8414.0
15	8763.0
16	8763.0
17	8763.0
18	14812.5
19	20862.0
20	20862.0
21	20862.0
22	31947.0
23	43032.0
24	43032.0
25	43032.0
26	64822.5
27	86613.0
28	86613.0
29	86613.0
30	102919.5
31	119226.0
32	119226.0
33	119226.0
34	139107.5
35	158989.0
36	158989.0
37	158989.0
38	175732.5
39	192476.0
40	192476.0
41	192476.0
42	209984.0
43	227492.0
44	227492.0
45	227492.0
46	242370.5
47	257249.0
48	257249.0
49	257249.0
50	255427.0
51	253605.0
52	253605.0
53	253605.0
54	235880.5
55	218156.0
56	218156.0
57	218156.0
58	190378.0
59	162600.0
60	162600.0
61	162600.0
62	135572.0
63	108544.0
64	108544.0
65	108544.0
66	82060.5
67	55577.0
68	55577.0
69	55577.0
70	41747.5
71	27918.0
72	27918.0
73	27918.0
74	19907.5
75	11897.0
76	11897.0
77	11897.0
78	9164.5
79	6432.0
80	6432.0
81	6432.0
82	4536.5
83	2641.0
84	2641.0
85	2641.0
86	1847.0
87	1053.0
88	1053.0
89	1053.0
90	856.0
91	659.0
92	659.0
93	659.0
94	348.5
95	38.0
96	38.0
97	38.0
98	21.0
99	4.0
100	4.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0294456800313415
2	0.0012605171246293449
3	2.52103424925869E-4
4	0.00398323411382873
5	0.03327765209021471
6	0.011596757546589972
7	0.020369956734010215
8	0.027176749207008678
9	0.03352975551514058
10	0.03685752072416205
11	0.03837014127371726
12	0.008319413022553677
13	0.002218510139347647
14	0.006201744253176377
15	0.006605109733057768
16	0.008016888912642635
17	0.009680771517153368
18	0.014369895220774531
19	0.017596819059825655
20	0.01563041234540388
21	0.011092550696738236
22	0.010638764531871672
23	0.008067309597627808
24	0.005294171923443249
25	0.10936246573284196
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1983313.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	46.387352207094246
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.36449012250614	34.95959147220768
2	12.792022053194518	11.86776064844902
3	4.5853217975263805	6.381028116141682
4	2.1640431943487815	4.015369353904888
5	1.2002509041822642	2.7838230714593006
6	0.7461731294217407	2.07677974571736
7	0.5074336651552397	1.647695290310498
8	0.36688807829971903	1.3615173206938431
9	0.2903929657883134	1.2123504704236647
>10	1.7110309617834834	14.938917028076057
>50	0.15913194945623538	5.112358210377001
>100	0.10550444520527917	9.17660885473424
>500	0.00491422374525222	1.4555360584831647
>1k	0.001965689498100888	1.3956591357418184
>5k	3.27614916350148E-4	1.1049369194875158
>10k+	1.09204972116716E-4	0.5100683037923985
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	10069	0.507685877115715	No Hit
TATCAACGCAGAGTACTTTTTTTTT	7981	0.4024074868666721	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	7309	0.3685247865566353	No Hit
GGTATCAACGCAGAGTACTTTTTTT	6522	0.32884370747330355	No Hit
GAGTACTTTTTTTTTTTTTTTTTTT	4078	0.20561555336953874	No Hit
ACGCAGAGTACTTTTTTTTTTTTTT	3309	0.1668420466159401	No Hit
GTGGTATCAACGCAGAGTACATGGG	2209	0.11137929313224891	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	5.0420684985173795E-5	0.0
2	0.0	0.0	0.0	5.0420684985173795E-5	0.0
3	0.0	0.0	0.0	5.0420684985173795E-5	0.0
4	0.0	0.0	0.0	5.0420684985173795E-5	0.0
5	0.0	0.0	0.0	5.0420684985173795E-5	0.0
6	0.0	0.0	0.0	5.0420684985173795E-5	0.0
7	0.0	0.0	0.0	5.0420684985173795E-5	0.0
8	0.0	0.0	0.0	5.0420684985173795E-5	0.0
9	0.0	0.0	0.0	5.0420684985173795E-5	0.0
10	0.0	0.0	0.0	5.0420684985173795E-5	0.0
11	0.0	0.0	0.0	5.0420684985173795E-5	0.0
12	0.0	0.0	0.0	5.0420684985173795E-5	0.0
13	0.0	0.0	0.0	5.0420684985173795E-5	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAACGCA	160	0.0	14.845091	4
TATAACG	165	0.0	14.39415	2
GTGCGAG	55	1.9615969E-4	13.817338	11
ATAACGC	185	0.0	13.351884	3
GCCGACC	50	0.0015027752	13.296505	13
TATCACG	115	3.783498E-10	13.217585	2
CGAAATC	80	2.0061052E-6	13.059068	13
AGGACCG	180	0.0	12.671006	5
GGTATCA	2885	0.0	12.418152	1
TACAACG	125	1.4115358E-9	12.160177	2
CCGAGTG	110	3.8096914E-8	12.092306	9
GTATAAC	300	0.0	12.037152	1
AAGTGGC	275	0.0	11.401892	7
CGCGCCA	100	1.9270065E-6	11.401605	10
TGGTCGG	100	1.9270065E-6	11.401605	10
ACCGAGT	135	4.7202775E-9	11.260845	8
CTACGCT	60	0.005872679	11.084333	4
CGTCTTA	60	0.0058881342	11.0804205	15
ATCGTAG	60	0.0058881342	11.0804205	13
TTTATAC	250	0.0	11.020438	3
>>END_MODULE