FastQCFastQC Report
Thu 26 May 2016
SRR1364302_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364302_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1234290
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT52490.42526472709006796No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA42950.34797332879631204No Hit
GTACTTTTTTTTTTTTTTTTTTTTT37380.3028461706730185No Hit
TATCAACGCAGAGTACTTTTTTTTT31230.25301995479182365No Hit
NNNNNNNNNNNNNNNNNNNNNNNNN29120.23592510674152753No Hit
GAACAAAAAAAAAAAAAAAAAAAAA25890.20975621612424958No Hit
GAAAAAAAAAAAAAAAAAAAAAAAA25760.20870297904058205No Hit
GGTATCAACGCAGAGTACTTTTTTT22110.17913132246068592No Hit
GAGTACTTTTTTTTTTTTTTTTTTT17840.14453653517406767No Hit
ACGCAGAGTACTTTTTTTTTTTTTT13720.11115702144552739No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCACG607.734161E-817.4062022
TATAACG1152.5465852E-1114.035042
GCACCGT500.001500059313.2990326
ACGAAAT500.001511585513.28553212
ATAACGC1301.4551915E-1113.1454083
GCCCCCG604.0626765E-412.67708318
CTAAGTG751.487774E-512.6585413
CGAAATC604.1286863E-412.65288713
CAACGCG550.003061091512.0944544
CGAGTGG550.003070427312.08953810
GAACAAA9250.011.9058721
GTATAAA1850.011.8032341
AACGCGG658.001631E-411.6957365
AGACGAA658.028971E-411.69098210
GTCTAAG1552.5465852E-1111.6376911
GTATTAG1406.85759E-1011.5283151
CGAAACC752.0573768E-411.40937418
ACGAAAC752.0573768E-411.40937417
GTATAAC2000.011.3926871
GACGAAA752.0942114E-411.38759811
Produced by FastQC (version 0.11.5)