FastQCFastQC Report
Thu 26 May 2016
SRR1364302_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364302_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1234290
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACTTTTTTTTTTTTTTTTTTTTT136081.102496171888292No Hit
GTATCAACGCAGAGTACTTTTTTTT132801.0759221900849882No Hit
TATCAACGCAGAGTACTTTTTTTTT105680.8562007307844997No Hit
GGTATCAACGCAGAGTACTTTTTTT94760.7677288157564268No Hit
GAGTACTTTTTTTTTTTTTTTTTTT73000.5914331315979228No Hit
ACGCAGAGTACTTTTTTTTTTTTTT48970.3967463075938394No Hit
GTGGTATCAACGCAGAGTACTTTTT20630.16714062335431706No Hit
GCAGAGTACTTTTTTTTTTTTTTTT17700.14340227985319495No Hit
ATCAACGCAGAGTACTTTTTTTTTT17630.14283515219275858No Hit
GTGGTATCAACGCAGAGTACATGGG16710.13538147436988066No Hit
GTATAACGCAGAGTACTTTTTTTTT16070.1301963071887482No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGCGC508.719234E-515.19844310
TATAACG2100.014.9318852
ACTCCGC456.762026E-414.7774628
TAACGCA2250.014.3581584
ATACGTC602.5610674E-514.253745
TGCACCG400.005269105514.253745
ATGCGTA602.570876E-514.24796413
TGCGTAG602.570876E-514.24796414
AATGCGT602.5718586E-514.24738712
TTAGACT954.8839865E-914.0025394
GGTATCA26450.013.47125151
ATAACGC2500.013.3024123
GTTCGCG500.001500857913.29809916
TATCACG905.38741E-712.6694772
GTGTATC1051.9868821E-812.6689641
GAATGCG751.4801708E-512.66485711
CGTTCGC550.003071114212.08918215
TGCCGCG550.003072046412.08869212
GGGCGCG658.020072E-411.6925337
TATACTG2550.011.5520515
Produced by FastQC (version 0.11.5)