##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1364302_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1234290
Sequences flagged as poor quality	0
Sequence length	25
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.43998817133737	31.0	30.0	34.0	26.0	34.0
2	29.466818980952613	31.0	30.0	34.0	25.0	34.0
3	29.509569874178677	31.0	30.0	34.0	25.0	34.0
4	32.84273387939625	35.0	33.0	37.0	26.0	37.0
5	32.4611663385428	35.0	33.0	37.0	25.0	37.0
6	32.363734616662214	35.0	32.0	37.0	23.0	37.0
7	32.10974649393579	35.0	32.0	37.0	21.0	37.0
8	31.994177219292062	35.0	32.0	37.0	19.0	37.0
9	32.72752432572572	37.0	32.0	39.0	15.0	39.0
10	32.81279764074893	37.0	32.0	39.0	17.0	39.0
11	32.74939195812978	37.0	32.0	39.0	16.0	39.0
12	32.73151852481994	37.0	32.0	39.0	17.0	39.0
13	32.752961621661036	37.0	32.0	39.0	16.0	39.0
14	33.6543049040339	38.0	32.0	41.0	16.0	41.0
15	33.684302716541495	38.0	33.0	41.0	16.0	41.0
16	33.62775198697227	38.0	32.0	41.0	16.0	41.0
17	33.627140299281365	38.0	33.0	41.0	13.0	41.0
18	33.57744695330919	38.0	32.0	41.0	11.0	41.0
19	33.50369200106944	39.0	32.0	41.0	10.0	41.0
20	33.42366056599341	39.0	32.0	41.0	10.0	41.0
21	33.34609370569315	38.0	32.0	41.0	8.0	41.0
22	33.25624448063259	38.0	32.0	41.0	2.0	41.0
23	32.980980158633706	38.0	32.0	41.0	2.0	41.0
24	32.90121284301096	38.0	32.0	41.0	2.0	41.0
25	32.73277916859085	38.0	32.0	41.0	2.0	41.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1101	1	0.5547880808673327
1101	2	0.6732777834445933
1101	3	-1.1250764997383769
1101	4	-0.1708294022630099
1101	5	0.7846320037726784
1101	6	1.049428475839818
1101	7	1.1755785850564457
1101	8	1.35217872231188
1101	9	1.943293390776759
1101	10	1.8712361974691945
1101	11	1.661733146874539
1101	12	1.798011358405489
1101	13	1.5303923078269364
1101	14	1.597417474737938
1101	15	1.6293870178184449
1101	16	1.4900838984833698
1101	17	1.492371043033799
1101	18	1.6096269026829049
1101	19	1.6273646039173215
1101	20	1.8455961166743577
1101	21	1.7757395334201789
1101	22	1.8164100416899984
1101	23	2.2218712003722203
1101	24	2.2064484481334006
1101	25	2.390550788009392
1102	1	1.1490924162331275
1102	2	1.0510880630149089
1102	3	1.1073026823843577
1102	4	1.1435425639826349
1102	5	1.3071144649329867
1102	6	1.535968107014348
1102	7	1.6360707147014644
1102	8	1.6774722042151389
1102	9	2.3201226272970814
1102	10	2.003576389419436
1102	11	1.9192266268013398
1102	12	1.922520949179372
1102	13	2.0032027266478707
1102	14	1.9819793878078258
1102	15	1.9936392285342777
1102	16	1.997144803109279
1102	17	1.926306732607344
1102	18	1.9968482779219912
1102	19	2.0243847305724927
1102	20	2.265471087588949
1102	21	2.2945915962608368
1102	22	2.3925765351169233
1102	23	2.7948879347027074
1102	24	2.6616185090712676
1102	25	2.6148736481990795
1103	1	-2.8100145132844467
1103	2	-2.5730528571288502
1103	3	-2.706428189350394
1103	4	-2.5897906037648895
1103	5	-5.228253421190342
1103	6	-10.072798171105227
1103	7	-10.2770041888655
1103	8	-11.029719104517113
1103	9	-11.048742108142342
1103	10	-9.978803625036207
1103	11	-12.163702056220234
1103	12	-11.469624322559351
1103	13	-11.981298441973951
1103	14	-12.036187030894098
1103	15	-12.17747383096981
1103	16	-6.778661123703451
1103	17	-4.686346672292178
1103	18	-4.327737985245186
1103	19	-4.426509556965602
1103	20	-8.76627667886552
1103	21	-5.791133831012633
1103	22	-9.54898989004285
1103	23	-10.867160543853515
1103	24	-12.65687502438907
1103	25	-13.913579374693985
1104	1	0.6245877377600415
1104	2	0.8075431591865119
1104	3	0.8393955829085336
1104	4	0.9021458793126271
1104	5	0.983026085978608
1104	6	1.1260069217466437
1104	7	1.0286263959996695
1104	8	1.2009336587366413
1104	9	1.611821991117715
1104	10	1.3271243236982642
1104	11	1.3069357397187673
1104	12	1.2003786015187003
1104	13	1.0367242865377904
1104	14	1.1998356282327407
1104	15	1.2878733661279185
1104	16	0.966441045565972
1104	17	1.0027767485817733
1104	18	1.1168879062269141
1104	19	1.2539011025378954
1104	20	1.4367162884789693
1104	21	1.2981620609055113
1104	22	0.9952999802569451
1104	23	1.531435898683391
1104	24	1.7407847219595496
1104	25	1.9517024023524954
1105	1	0.5386989844897201
1105	2	0.6347753479275653
1105	3	0.1807403858378933
1105	4	0.4828638744947611
1105	5	0.733473487564595
1105	6	0.7758733298607012
1105	7	0.7895187553907341
1105	8	0.6728315016368178
1105	9	0.9417946110669959
1105	10	0.4393445956990192
1105	11	0.9272070346888555
1105	12	1.0557837464274087
1105	13	0.9552583797076011
1105	14	0.6383377052259576
1105	15	0.4581058649732981
1105	16	0.7130375072006032
1105	17	0.9692235043743977
1105	18	1.1943383369543241
1105	19	0.9440982768390285
1105	20	1.1610968407469926
1105	21	1.2760610621754864
1105	22	0.8239697650233637
1105	23	1.4414879988572409
1105	24	1.3937731263514763
1105	25	1.584166796596996
1106	1	0.09331253777562054
1106	2	0.42096621442266624
1106	3	0.19579052569488553
1106	4	-1.244985737301505
1106	5	-0.12836016876043033
1106	6	0.22021624747804225
1106	7	0.4813453609049141
1106	8	0.5949354981418686
1106	9	1.0358106501842244
1106	10	0.7762822071606976
1106	11	0.8908694467753335
1106	12	1.0278321228997953
1106	13	0.8181948901335758
1106	14	0.09383304173510965
1106	15	0.6494674170063277
1106	16	0.5787363807528791
1106	17	0.4977188267910222
1106	18	0.6526567253871747
1106	19	-0.7825382409059642
1106	20	0.47050628646961457
1106	21	0.09424788093237169
1106	22	0.6494099179136725
1106	23	0.6789605354716741
1106	24	0.6368238044521846
1106	25	0.9490942177958885
1107	1	0.19557377433445922
1107	2	0.06098232606024112
1107	3	0.09102516309893005
1107	4	0.06955146432012782
1107	5	0.3065975054816974
1107	6	0.2721608585838595
1107	7	0.18971724441515647
1107	8	-0.15527243917600586
1107	9	0.6047500483715709
1107	10	0.5910918300324965
1107	11	0.5933251631357521
1107	12	0.5288582585179142
1107	13	0.3589749027313047
1107	14	0.4579499763371899
1107	15	0.29145309222786153
1107	16	0.3285733018226722
1107	17	0.1754818698345133
1107	18	0.2756191733093942
1107	19	0.5250403033272093
1107	20	0.3231385392765347
1107	21	0.41955717016990945
1107	22	0.6758628156720619
1107	23	0.6028835136200357
1107	24	0.9916933693445174
1107	25	0.766582727506794
1108	1	0.0031493213726001557
1108	2	0.11038173241433569
1108	3	0.22051176360766078
1108	4	-0.004213285155898916
1108	5	0.5623255322845395
1108	6	0.2112175868924311
1108	7	-0.049027414508636014
1108	8	0.35166082776779817
1108	9	0.5720059221079481
1108	10	0.4407504257395658
1108	11	0.46315826294777196
1108	12	0.48247147003417723
1108	13	0.0024689218839100135
1108	14	0.007943800567048243
1108	15	-0.13354603930446274
1108	16	-0.6446364003122085
1108	17	-0.6992146489610604
1108	18	-0.6743242797952789
1108	19	-0.604514809631489
1108	20	0.2089234603617669
1108	21	0.15276390021541175
1108	22	0.23629948121937616
1108	23	-0.17294375008921037
1108	24	0.08518005477008472
1108	25	0.025173802746977003
1109	1	0.15741378034705633
1109	2	0.17901325857362593
1109	3	0.08793253014401614
1109	4	0.12046705301683147
1109	5	0.27004507176055625
1109	6	0.5178678473682794
1109	7	0.48624325887392317
1109	8	0.3660028206165755
1109	9	1.3795515233598437
1109	10	0.6458925344193105
1109	11	0.7303183618643558
1109	12	0.7710173886705931
1109	13	0.7503495891393186
1109	14	0.4275951762036172
1109	15	0.22262339800442277
1109	16	0.5601818515031596
1109	17	0.529987795379192
1109	18	0.37328291898427324
1109	19	0.7961447940309938
1109	20	1.0373207931811521
1109	21	1.0046853230952948
1109	22	0.5987981507933213
1109	23	1.0448472344644202
1109	24	0.9012192877159393
1109	25	0.9167560652671227
1110	1	0.2000481628123829
1110	2	0.3407243839728693
1110	3	0.09990842154029878
1110	4	0.08791625610516007
1110	5	0.46155740049903926
1110	6	0.5607479159512962
1110	7	0.5500040337783965
1110	8	0.5212267870359888
1110	9	-2.930232534224796
1110	10	-0.9689709529644475
1110	11	-0.14219667987615026
1110	12	0.3893832100724737
1110	13	-0.02488420472479902
1110	14	0.30824399664888347
1110	15	0.536803668886435
1110	16	-0.01461650292258554
1110	17	0.21365907780845106
1110	18	0.25023337925369304
1110	19	0.14415917973728654
1110	20	0.627490212055072
1110	21	0.3685464633925193
1110	22	0.492238331451027
1110	23	0.7955385533806876
1110	24	0.9964226583203768
1110	25	0.9080525038638854
1111	1	0.10887318725031037
1111	2	0.2151075706786365
1111	3	0.14059854861752186
1111	4	0.13073346040623335
1111	5	0.06207010822141967
1111	6	0.12572054547231204
1111	7	0.22060276300615556
1111	8	0.4209359756967892
1111	9	1.1180680522854658
1111	10	0.5748648241616507
1111	11	0.7884797619615824
1111	12	0.7553836988310252
1111	13	0.5621079751383462
1111	14	0.32023275520215577
1111	15	0.6675930139499755
1111	16	0.43890304741954367
1111	17	0.4947656271730665
1111	18	0.39345185432700447
1111	19	0.5676508708418808
1111	20	0.9048997917227126
1111	21	0.7436041369018085
1111	22	0.7635154575507315
1111	23	1.1769891887668038
1111	24	0.9987559916537094
1111	25	1.216663940814037
1112	1	-0.16446630941121754
1112	2	-0.2372118951662543
1112	3	-0.2510367877974744
1112	4	-0.15174111586495087
1112	5	-0.21719568530708244
1112	6	-0.2046148423037515
1112	7	0.019651299215709628
1112	8	-0.3505445070972506
1112	9	0.2680046213661029
1112	10	-0.08246234584348144
1112	11	-0.03273133202917222
1112	12	-0.502857954995374
1112	13	-0.36689921541121606
1112	14	-1.1388789551214202
1112	15	-0.3176291226704677
1112	16	-0.6059066598223666
1112	17	-0.6667690415480436
1112	18	-0.7646696145995477
1112	19	-0.4671452257169264
1112	20	-0.37671808192905587
1112	21	-0.5666557501038412
1112	22	-0.4639722157928716
1112	23	-0.15333233152081505
1112	24	-0.287347244093592
1112	25	-0.15775593591940051
1113	1	-1.9718715574944312
1113	2	-1.764393930822866
1113	3	-1.970050100031127
1113	4	-2.000623896951126
1113	5	-1.962222684571369
1113	6	-2.0243004755487064
1113	7	-2.1933536934503017
1113	8	-2.4644318921710777
1113	9	-11.629879395161982
1113	10	-5.163945986649157
1113	11	-3.480655373173555
1113	12	-13.979942126994803
1113	13	-5.118681814651442
1113	14	-4.566642620173219
1113	15	-4.401093172236212
1113	16	-3.8912335892170944
1113	17	-3.8337989373914994
1113	18	-3.5273814790063263
1113	19	-4.716949729758717
1113	20	-16.146421529598605
1113	21	-18.401032499734832
1113	22	-13.918230788695514
1113	23	-20.271971772194156
1113	24	-21.317391155493432
1113	25	-18.18401023336014
1114	1	-0.45793733341528053
1114	2	-0.44432023393757447
1114	3	-0.30583516045386716
1114	4	-0.4731108766844798
1114	5	-0.3862844274736936
1114	6	-0.391899959090221
1114	7	-0.3788725456358186
1114	8	-0.39151839896772955
1114	9	0.18042366151788514
1114	10	-0.41325326494194314
1114	11	-0.6589931312214574
1114	12	-0.7043041701974246
1114	13	-0.6686665820275124
1114	14	-0.8004996656244643
1114	15	-0.653446975314651
1114	16	-0.7323210534929601
1114	17	-0.6122295419090591
1114	18	-0.8698398794357445
1114	19	-0.9612229885247316
1114	20	-1.3012140032316601
1114	21	-1.3639664481760256
1114	22	-1.6333371994540968
1114	23	-1.3705921316840808
1114	24	-0.8460357421090343
1114	25	-1.2030727741618108
1115	1	-0.39142812279335715
1115	2	-0.2197701585790064
1115	3	-0.3110302723431779
1115	4	-0.204401910772809
1115	5	-0.5067661363200635
1115	6	-0.6239126423006098
1115	7	-0.8613513854654613
1115	8	-0.6173063823818126
1115	9	-0.13767212238702342
1115	10	-0.3007334291134569
1115	11	-0.10534556554933516
1115	12	-0.42267525313404164
1115	13	-0.6105514135079417
1115	14	-0.5280576378109458
1115	15	-0.7214572043906387
1115	16	-0.5870205333664842
1115	17	-0.5694527134382881
1115	18	-0.7508167046249596
1115	19	-0.612528168459427
1115	20	-1.0942050117707325
1115	21	-0.736011583622215
1115	22	-1.0289714420125833
1115	23	-0.8836090645083061
1115	24	-0.2614012135864634
1115	25	-0.13046924695888862
1116	1	-2.736538577455569
1116	2	-2.4781865469684234
1116	3	-2.4602249807942442
1116	4	-2.505619480770239
1116	5	-4.187076950602108
1116	6	-3.609161807468862
1116	7	-5.258073707582078
1116	8	-3.8895052007737974
1116	9	-2.6671378300674746
1116	10	-4.754311646426583
1116	11	-3.860814355685477
1116	12	-5.0799986541101525
1116	13	-4.760656730744007
1116	14	-4.1136201859743124
1116	15	-5.616083456638261
1116	16	-5.699302834933398
1116	17	-6.692763784591641
1116	18	-5.97993049861417
1116	19	-5.508113835040469
1116	20	-5.476864914159638
1116	21	-4.765572333686428
1116	22	-5.049631601272797
1116	23	-5.238069634762606
1116	24	-6.299655773052169
1116	25	-5.7721890003624345
1201	1	1.094148770305999
1201	2	1.0550514069470793
1201	3	0.6243386723681184
1201	4	1.0192751072410715
1201	5	1.3341398369220414
1201	6	1.3441741389992075
1201	7	1.4639594978943045
1201	8	1.359647303635871
1201	9	1.9717803321521998
1201	10	1.7687115638094397
1201	11	1.6716144597245517
1201	12	2.011519110539737
1201	13	1.8134211593363503
1201	14	2.0243443687195324
1201	15	1.9421610767772037
1201	16	1.6978066647446255
1201	17	1.6522315957594529
1201	18	1.754547761038097
1201	19	1.6618517275767744
1201	20	1.984514261023051
1201	21	1.9357469260498306
1201	22	1.904883615570725
1201	23	2.2677612020937943
1201	24	2.2395608464847427
1201	25	2.287165368705516
1202	1	1.0840359779479876
1202	2	1.1092005939344496
1202	3	1.0958078509431033
1202	4	1.1660357859876243
1202	5	1.3589422012446803
1202	6	1.299511243146732
1202	7	1.2668236932387167
1202	8	1.3099476036037707
1202	9	2.0538471220529075
1202	10	1.6701206381151437
1202	11	1.7414565061476281
1202	12	1.8216976523193935
1202	13	1.6529684402546252
1202	14	1.7454769412486684
1202	15	1.7092790604616042
1202	16	1.5779611869544254
1202	17	1.794021441126553
1202	18	1.8529285985130528
1202	19	1.850627615027932
1202	20	2.056295140559925
1202	21	2.042115764808784
1202	22	2.09592243429492
1202	23	2.373175235278431
1202	24	2.3958025032816224
1202	25	2.590163940814037
1203	1	1.4163917057688309
1203	2	1.3482186817897457
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2211	13	0.49677847322264057
2211	14	0.43516953681135107
2211	15	0.35214282237909345
2211	16	0.3306520895651346
2211	17	0.2192713742995025
2211	18	0.15987905739213915
2211	19	-0.00811158126539624
2211	20	0.5604361901901456
2211	21	0.5440332556757497
2211	22	0.5195863387767901
2211	23	0.7771117941307892
2211	24	0.8661544590866654
2211	25	1.0490719867910485
2212	1	-0.02810720490655072
2212	2	0.16357815891392846
2212	3	0.25408874469595233
2212	4	0.38418444079839276
2212	5	0.01929559841750006
2212	6	0.23250485919780317
2212	7	0.24300472379047022
2212	8	0.14069087765757615
2212	9	0.9361955032658571
2212	10	0.47284521631851817
2212	11	0.6897738796086443
2212	12	0.3792170321643553
2212	13	0.2696373869030566
2212	14	0.3793209904962751
2212	15	0.3522498766950761
2212	16	0.4978540278116981
2212	17	0.19498131344757041
2212	18	0.24418714844465228
2212	19	0.5150234198614925
2212	20	0.5763703799580071
2212	21	0.9390355094508251
2212	22	0.43928996735465375
2212	23	0.8880087966099381
2212	24	0.9449520700850869
2212	25	0.9617325682650133
2213	1	-0.17565254988112144
2213	2	-0.3088694408156165
2213	3	-0.3204019511326024
2213	4	-0.34541546513099775
2213	5	-0.13144888228332263
2213	6	-0.10772723063962886
2213	7	-0.33609763679394433
2213	8	-0.11680390436318078
2213	9	0.32681342959681103
2213	10	-0.12974686999127272
2213	11	-0.22408095767859493
2213	12	-0.020183267685716544
2213	13	-0.5142993212134748
2213	14	-0.31220677503272753
2213	15	-0.2788750020420281
2213	16	-0.607920290911288
2213	17	-0.40266765618525824
2213	18	-0.47540746601282535
2213	19	-0.5411367353550176
2213	20	0.026474004616268587
2213	21	-0.2111724747923489
2213	22	-0.1522444125142357
2213	23	-0.5533896218278969
2213	24	-0.3721430588210488
2213	25	-0.09679258092509357
2214	1	-1.1356633981155433
2214	2	-0.898563161028683
2214	3	-1.0312591749597146
2214	4	-0.7438762956913294
2214	5	-1.0799989974696338
2214	6	-0.9966737634707741
2214	7	-1.0483809768312433
2214	8	-1.1234949186121526
2214	9	-10.884667720072258
2214	10	-4.3409400538871274
2214	11	-2.0528373112091494
2214	12	-1.4015756507624708
2214	13	-1.1479977159185637
2214	14	-1.3116940740661356
2214	15	-1.3150086120662863
2214	16	-1.7164871964828947
2214	17	-1.5573441289244982
2214	18	-1.9135603407949446
2214	19	-2.222548316149986
2214	20	-1.7432912651878532
2214	21	-2.5535218793583567
2214	22	-2.3564357619614604
2214	23	-2.8404823559486445
2214	24	-2.986809048996694
2214	25	-10.405669392519297
2215	1	-0.716054551677427
2215	2	-0.4295590959880329
2215	3	-0.3715180014990267
2215	4	-0.5138959102231411
2215	5	-0.5214112437599283
2215	6	-0.4840486852969157
2215	7	-0.4985522486488563
2215	8	-0.36754304528222903
2215	9	0.2796892644066773
2215	10	0.2523426796395114
2215	11	0.3969040043858243
2215	12	0.45310980605712814
2215	13	0.20257650660688142
2215	14	0.019688466157866458
2215	15	-0.4083801795232205
2215	16	-0.2359489339324412
2215	17	-0.15559800919056954
2215	18	-0.12644907807392514
2215	19	-0.21716497997396544
2215	20	-0.10074123391831336
2215	21	-0.10647045517278286
2215	22	-0.03503815550288181
2215	23	0.2367747365523556
2215	24	0.5681009799987038
2215	25	0.5187187193688132
2216	1	-2.503868716576971
2216	2	-2.4993988081241127
2216	3	-2.583423041176392
2216	4	-2.421480474824385
2216	5	-4.200833228383093
2216	6	-4.411522830386371
2216	7	-3.418938453873139
2216	8	-4.990087086128529
2216	9	-3.5190364619441716
2216	10	-3.919376588988495
2216	11	-3.046207186026642
2216	12	-4.452447998911861
2216	13	-4.218980346745852
2216	14	-4.50235654803631
2216	15	-4.1501376043032145
2216	16	-5.184920799489191
2216	17	-6.03370934829308
2216	18	-5.3196614920910505
2216	19	-6.680627833770483
2216	20	-5.728927489925958
2216	21	-5.612285951420077
2216	22	-5.46590014598214
2216	23	-5.033287553137022
2216	24	-4.995562948483677
2216	25	-5.434762457615797
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	47280.0
3	3.0
4	724.0
5	6570.0
6	9030.0
7	7337.0
8	5679.0
9	4578.0
10	4025.0
11	3683.0
12	3250.0
13	2957.0
14	2920.0
15	3095.0
16	3115.0
17	3257.0
18	3724.0
19	4659.0
20	6167.0
21	8217.0
22	9810.0
23	11231.0
24	13333.0
25	14907.0
26	16468.0
27	18478.0
28	20488.0
29	24010.0
30	29114.0
31	36089.0
32	44399.0
33	55056.0
34	68736.0
35	87230.0
36	113931.0
37	172166.0
38	368574.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.92653202467869	19.137980078431756	12.386349730416171	23.549138166473384
2	16.382396072172213	22.450679348926897	38.37474782665057	22.792176752250317
3	18.9839154102733	28.138755411018668	29.92699428901058	22.95033488969745
4	11.922148610207502	17.749335830938488	37.44730601361638	32.88120954523763
5	11.622072155666544	39.62005590082749	34.927320009951615	13.830551933554355
6	28.7399609782658	38.50284884587711	18.940530747441226	13.816659428415859
7	25.978284915073907	33.26918261723577	23.163945730925157	17.588586736765166
8	25.931373502411702	35.12776502774753	21.115427752709923	17.825433717130853
9	26.539745471432198	15.413623105481935	20.675441632107912	37.37118979097795
10	14.669369408068833	31.003745312280913	33.16574931776312	21.16113596188713
11	33.250962034098315	23.696350592133204	24.12241742067879	18.93026995308969
12	22.13964108127729	27.87571290715415	31.070962974606363	18.913683036962194
13	27.934924833807973	22.47053914677966	27.630533151308672	21.964002868103687
14	21.150650480740797	21.853187505519585	30.331839567799157	26.664322445940456
15	22.986134686294545	29.262350402801417	25.984600937749903	21.766913973154132
16	21.267812304274305	29.00568713543874	27.84840895519244	21.878091605094518
17	20.67889830947936	28.333722256793198	29.18595078821821	21.80142864550923
18	20.051096434188427	26.780657252800943	32.14254171133351	21.025704601677123
19	21.540120783300694	26.359136252735777	31.962440979782208	20.138301984181325
20	21.546339961430633	26.013256194597044	32.114022720274846	20.326381123697473
21	22.246250752523334	25.367267848070906	31.789421486799746	20.59705991260601
22	21.645250339698407	25.723413788716908	31.67901356605949	20.952322305525193
23	21.157649537158946	25.68940023091414	32.098361319856586	21.054588912070326
24	20.993639862264533	26.05152926878671	32.1643103099048	20.790520559043955
25	20.994260978301682	25.67030265088145	32.2762191062307	21.059217264586174
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1371.0
1	1371.0
2	2068.0
3	2765.0
4	2765.0
5	2765.0
6	9468.0
7	16171.0
8	16171.0
9	16171.0
10	14322.5
11	12474.0
12	12474.0
13	12474.0
14	10751.0
15	9028.0
16	9028.0
17	9028.0
18	14861.0
19	20694.0
20	20694.0
21	20694.0
22	30474.5
23	40255.0
24	40255.0
25	40255.0
26	56297.5
27	72340.0
28	72340.0
29	72340.0
30	83192.0
31	94044.0
32	94044.0
33	94044.0
34	103467.0
35	112890.0
36	112890.0
37	112890.0
38	119512.0
39	126134.0
40	126134.0
41	126134.0
42	133726.5
43	141319.0
44	141319.0
45	141319.0
46	146208.0
47	151097.0
48	151097.0
49	151097.0
50	145953.5
51	140810.0
52	140810.0
53	140810.0
54	126886.0
55	112962.0
56	112962.0
57	112962.0
58	96199.5
59	79437.0
60	79437.0
61	79437.0
62	64922.5
63	50408.0
64	50408.0
65	50408.0
66	38565.5
67	26723.0
68	26723.0
69	26723.0
70	19962.5
71	13202.0
72	13202.0
73	13202.0
74	9313.0
75	5424.0
76	5424.0
77	5424.0
78	4152.0
79	2880.0
80	2880.0
81	2880.0
82	2010.0
83	1140.0
84	1140.0
85	1140.0
86	801.0
87	462.0
88	462.0
89	462.0
90	349.0
91	236.0
92	236.0
93	236.0
94	129.5
95	23.0
96	23.0
97	23.0
98	12.0
99	1.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.028437401259023408
2	0.0016203647441038976
3	2.4305471161558466E-4
4	0.0033217477254129904
5	0.02584481766845717
6	0.008587933143750658
7	0.01758095747352729
8	0.024791580584789637
9	0.03248831311928315
10	0.03896977209569874
11	0.03613413379351692
12	0.006724513688031176
13	0.0020254559301298722
14	0.003969893623054549
15	0.004780075995106498
16	0.008182841957724684
17	0.009236079041392217
18	0.012395790292394819
19	0.014502264459729885
20	0.012152735580779233
21	0.008830987855366243
22	0.008668951380955853
23	0.005266185418337668
24	0.0032407294882077953
25	0.0934140274975897
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1234290.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	51.352749964497676
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.48981449234633	40.306678183852675
2	12.082694122586432	12.409591403493735
3	3.7964050770189854	5.848675220523165
4	1.7073257049084034	3.507034801284839
5	0.8925773328934173	2.2918150300026934
6	0.5883028321107528	1.812658094447361
7	0.3815766439741102	1.3716506993206241
8	0.28436952557817374	1.1682525715631027
9	0.21461131116703208	0.9918792901722246
>10	1.3586480606305291	13.414492408346723
>50	0.13972602963522598	4.997363321249293
>100	0.05950577229570649	5.424139338550249
>500	0.0025389113456494093	0.8305211065115722
>1k	0.0011107737137216165	1.2060157355152343
>5k	3.1736391820617616E-4	1.3670351337164575
>10k+	4.760458773092642E-4	3.0521976614499065
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GTACTTTTTTTTTTTTTTTTTTTTT	13608	1.102496171888292	No Hit
GTATCAACGCAGAGTACTTTTTTTT	13280	1.0759221900849882	No Hit
TATCAACGCAGAGTACTTTTTTTTT	10568	0.8562007307844997	No Hit
GGTATCAACGCAGAGTACTTTTTTT	9476	0.7677288157564268	No Hit
GAGTACTTTTTTTTTTTTTTTTTTT	7300	0.5914331315979228	No Hit
ACGCAGAGTACTTTTTTTTTTTTTT	4897	0.3967463075938394	No Hit
GTGGTATCAACGCAGAGTACTTTTT	2063	0.16714062335431706	No Hit
GCAGAGTACTTTTTTTTTTTTTTTT	1770	0.14340227985319495	No Hit
ATCAACGCAGAGTACTTTTTTTTTT	1763	0.14283515219275858	No Hit
GTGGTATCAACGCAGAGTACATGGG	1671	0.13538147436988066	No Hit
GTATAACGCAGAGTACTTTTTTTTT	1607	0.1301963071887482	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	2.4305471161558466E-4	0.0	0.0	0.0	0.0
11	2.4305471161558466E-4	0.0	0.0	0.0	0.0
12	2.4305471161558466E-4	0.0	0.0	0.0	0.0
13	2.4305471161558466E-4	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTGCGC	50	8.719234E-5	15.198443	10
TATAACG	210	0.0	14.931885	2
ACTCCGC	45	6.762026E-4	14.777462	8
TAACGCA	225	0.0	14.358158	4
ATACGTC	60	2.5610674E-5	14.25374	5
TGCACCG	40	0.0052691055	14.25374	5
ATGCGTA	60	2.570876E-5	14.247964	13
TGCGTAG	60	2.570876E-5	14.247964	14
AATGCGT	60	2.5718586E-5	14.247387	12
TTAGACT	95	4.8839865E-9	14.002539	4
GGTATCA	2645	0.0	13.4712515	1
ATAACGC	250	0.0	13.302412	3
GTTCGCG	50	0.0015008579	13.298099	16
TATCACG	90	5.38741E-7	12.669477	2
GTGTATC	105	1.9868821E-8	12.668964	1
GAATGCG	75	1.4801708E-5	12.664857	11
CGTTCGC	55	0.0030711142	12.089182	15
TGCCGCG	55	0.0030720464	12.088692	12
GGGCGCG	65	8.020072E-4	11.692533	7
TATACTG	255	0.0	11.552051	5
>>END_MODULE