FastQCFastQC Report
Thu 26 May 2016
SRR1364301_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364301_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2065742
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT61450.297471804320191No Hit
NNNNNNNNNNNNNNNNNNNNNNNNN57840.2799962434805508No Hit
TATCAACGCAGAGTACTTTTTTTTT39770.19252162177077292No Hit
GTACTTTTTTTTTTTTTTTTTTTTT33100.16023298165985878No Hit
GGTATCAACGCAGAGTACTTTTTTT27500.13312407841831167No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCACG551.9464665E-413.83000318
TAACGCA1257.2759576E-1213.6860414
TGCCGTA655.410575E-513.16509915
GGTATCA17800.012.9600131
CGTCTCG1051.9703293E-812.67781217
ATAACGC1457.2759576E-1212.4395543
GTTATCA3900.011.6841141
TATAACG1552.5465852E-1111.6370012
CTTTACG907.3910196E-611.62132617
TTTACGG907.3930223E-611.62104418
GTATCAA44100.011.1078581
AACGCAT3100.011.0317636
ACTGCGT909.524259E-510.5548268
TCGAGCC1451.4713805E-810.47007213
GTCTTAT3000.010.44267751
ACGCATA3150.010.2532617
GTCTAGA2500.010.25281051
CATGGGT11850.010.1859314
GTATAAA3650.010.1435731
ATGGGTA6000.010.1378085
Produced by FastQC (version 0.11.5)