##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1364301_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2065742
Sequences flagged as poor quality	0
Sequence length	25
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	25.655302065795244	30.0	25.0	31.0	2.0	34.0
2	25.55129875850905	30.0	25.0	31.0	2.0	34.0
3	24.981929495551718	30.0	23.0	31.0	2.0	34.0
4	28.70066542675707	35.0	27.0	37.0	2.0	37.0
5	29.16933866862367	35.0	28.0	37.0	2.0	37.0
6	29.41385758724952	35.0	30.0	37.0	2.0	37.0
7	28.992657359922003	35.0	28.0	37.0	2.0	37.0
8	28.512622583071845	35.0	27.0	37.0	2.0	37.0
9	29.54473501531169	35.0	27.0	39.0	2.0	39.0
10	28.865162251626778	35.0	25.0	39.0	2.0	39.0
11	29.48354780025773	35.0	27.0	39.0	2.0	39.0
12	28.995111199752923	35.0	27.0	39.0	2.0	39.0
13	28.641286762819366	35.0	25.0	39.0	2.0	39.0
14	28.907784708835855	35.0	22.0	40.0	2.0	41.0
15	29.561245305560906	36.0	27.0	40.0	2.0	41.0
16	29.92058156342854	36.0	27.0	40.0	2.0	41.0
17	30.080116006742372	36.0	27.0	40.0	2.0	41.0
18	29.908636702937734	36.0	27.0	40.0	2.0	41.0
19	29.88747287899457	37.0	27.0	40.0	2.0	41.0
20	29.716567703033583	37.0	27.0	40.0	2.0	41.0
21	29.65116747396335	37.0	27.0	40.0	2.0	41.0
22	29.714527758064655	37.0	27.0	40.0	2.0	41.0
23	29.703170579869123	37.0	27.0	40.0	2.0	41.0
24	29.525928697775424	37.0	27.0	40.0	2.0	41.0
25	29.35463867220592	37.0	27.0	40.0	2.0	41.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1101	1	0.32755561770812847
1101	2	0.39347744175086063
1101	3	1.149202958620151
1101	4	0.4850284493049628
1101	5	0.08957460966377084
1101	6	-0.17011420897601326
1101	7	0.20587515086313246
1101	8	0.3658777834571012
1101	9	0.42588429644720094
1101	10	0.6286626262310016
1101	11	0.186186189830714
1101	12	0.3714601047647754
1101	13	0.3479458291106887
1101	14	0.7368265056466896
1101	15	0.05406941755797945
1101	16	-0.31761208405859165
1101	17	-0.9384172240156978
1101	18	-6.276770525483318
1101	19	-7.654376569566363
1101	20	-8.9159818399444
1101	21	-10.749382692174258
1101	22	-10.969333324662262
1101	23	-12.1147134438847
1101	24	-12.05057401295836
1101	25	-12.391581772062906
1102	1	1.441025637211382
1102	2	1.6377243284839444
1102	3	2.44352116913349
1102	4	1.901912751289995
1102	5	1.11360802513952
1102	6	1.2868366783749927
1102	7	1.4595494519717818
1102	8	1.6664606034622231
1102	9	1.930510662331013
1102	10	-1.6873231595164206
1102	11	0.5304742220420309
1102	12	1.4763286892761514
1102	13	2.0028544945662645
1102	14	2.3858829076305845
1102	15	2.0304323058220106
1102	16	1.7089165001656106
1102	17	1.4913199152991297
1102	18	1.7180570274302127
1102	19	1.344200222700465
1102	20	1.7284912231359257
1102	21	1.7648680516379507
1102	22	1.733353410699035
1102	23	1.6891904747393482
1102	24	1.9710110732664212
1102	25	2.000884693877204
1103	1	-0.8499211557571833
1103	2	-0.29815232294480865
1103	3	-3.647402060047906
1103	4	-0.9812532791544157
1103	5	-3.7154382698476773
1103	6	-7.960366300087703
1103	7	-8.975161577451534
1103	8	-2.8977035890067917
1103	9	-8.782141520461138
1103	10	-4.68263082403093
1103	11	-9.46673268371853
1103	12	-3.2064681751692348
1103	13	-0.6768146698618445
1103	14	-0.0637581373448306
1103	15	-0.9847427731166754
1103	16	-1.3172503000291833
1103	17	-1.5264754567730705
1103	18	-1.0716534521714003
1103	19	-6.214707674310073
1103	20	-1.9017709632693425
1103	21	-7.308310074883877
1103	22	-3.3367032994820924
1103	23	-2.1999497612127072
1103	24	-7.867771778580195
1103	25	-4.6919927587203105
1104	1	1.5511940406652052
1104	2	1.578706727578954
1104	3	2.360274271030711
1104	4	1.902581986125739
1104	5	1.4873709274042497
1104	6	1.362722569466122
1104	7	1.4402366156558308
1104	8	1.6667991101053552
1104	9	1.7870093896900805
1104	10	2.028495568015501
1104	11	1.609718771244811
1104	12	1.951898448773182
1104	13	2.1040520398409264
1104	14	2.6449404571209776
1104	15	1.6325539982399917
1104	16	1.1112505154737917
1104	17	0.792010420835993
1104	18	0.8229112126927376
1104	19	1.2658590300911463
1104	20	-8.854817950586167
1104	21	-3.2507929462028073
1104	22	-0.8861932592838784
1104	23	0.04431131200792038
1104	24	0.6769311943413641
1104	25	1.0399094682421186
1105	1	1.4107363466481964
1105	2	1.2635937133375386
1105	3	2.1702796295294142
1105	4	1.6382155366363094
1105	5	1.2859462131463637
1105	6	1.3002626235402772
1105	7	1.4596391173303012
1105	8	1.820090510428301
1105	9	1.8238254107179905
1105	10	2.2938377281999642
1105	11	1.9078334745634393
1105	12	2.1343686749414275
1105	13	2.083195046922473
1105	14	2.8325326548906347
1105	15	2.173164498276922
1105	16	1.7166520685163782
1105	17	0.9965352221634198
1105	18	1.5313079442511714
1105	19	1.5343579584399336
1105	20	1.4090359426334444
1105	21	1.6006204755116222
1105	22	1.5970256911472411
1105	23	1.4483852074617758
1105	24	1.4449689579266547
1105	25	1.4236861936452172
1106	1	0.8250125923704807
1106	2	1.1403294529894374
1106	3	1.7368587994981262
1106	4	1.3130490598247277
1106	5	0.49724944379060787
1106	6	0.22899883414434896
1106	7	0.8626269542533507
1106	8	0.7721892557909946
1106	9	1.1617268207456064
1106	10	-2.2283254125011815
1106	11	-0.23235468318083363
1106	12	0.367714718405292
1106	13	1.2127463394710283
1106	14	0.45400504581759193
1106	15	1.1103718714276276
1106	16	0.5843992755747642
1106	17	-6.128244093712809
1106	18	-2.205933515158687
1106	19	-0.6153202640861615
1106	20	0.0968367872395497
1106	21	0.47638094174630297
1106	22	0.5062989665239748
1106	23	0.1185585154704718
1106	24	0.5038052080158018
1106	25	0.676665304804672
1107	1	0.3594194412238636
1107	2	0.7229009059735887
1107	3	1.2055374473859857
1107	4	0.7039981111531546
1107	5	0.24282412942595855
1107	6	0.45289553171345176
1107	7	0.2744695654380358
1107	8	0.46105251132234315
1107	9	0.5229316834884727
1107	10	-2.677438450861029
1107	11	-0.5788084980415356
1107	12	-0.24028953483329119
1107	13	0.7225724598623664
1107	14	1.0710165760815684
1107	15	0.8353117042411711
1107	16	-5.699840244416883
1107	17	-2.24946376684003
1107	18	-0.5896423297108413
1107	19	0.3021462582741954
1107	20	0.7090847852268567
1107	21	0.7133957473837427
1107	22	0.4746121017209468
1107	23	0.24211129882587912
1107	24	0.5538602722653394
1107	25	0.7501250485660336
1108	1	-0.4737165812767472
1108	2	-3.1574928546991323
1108	3	-0.2817629099259982
1108	4	-0.9166126196861697
1108	5	-0.8710170461161084
1108	6	-0.5322344064385689
1108	7	-0.18519292541391508
1108	8	0.059047833261871574
1108	9	-0.5274973778859611
1108	10	-3.3699348992660383
1108	11	-1.6534852670228375
1108	12	-5.890898687185956
1108	13	-1.9516027808568523
1108	14	-5.984836739457915
1108	15	-2.7859734708990977
1108	16	-7.561561225374593
1108	17	-3.25908894800725
1108	18	-2.082944523807299
1108	19	-0.6958698709895117
1108	20	-0.6827996408497441
1108	21	-0.29379712933644697
1108	22	-0.42656745831176934
1108	23	-0.35779255698653856
1108	24	-0.2425831102880771
1108	25	-0.5200724057654753
1109	1	-8.407621059825804
1109	2	-6.292509300542008
1109	3	-5.437058399983574
1109	4	-5.483171906771368
1109	5	-2.870959967268817
1109	6	-2.04800243800317
1109	7	-1.5913231936131496
1109	8	-1.089763856486055
1109	9	-6.124419044302908
1109	10	-2.0946561047712926
1109	11	-1.731362219297786
1109	12	-6.472310960565093
1109	13	-8.325649385735666
1109	14	-8.34969008249465
1109	15	-4.374816696710152
1109	16	-2.59246050261369
1109	17	-2.254030335165936
1109	18	-1.66304219998473
1109	19	-1.300058388152408
1109	20	-1.101872537672591
1109	21	-1.0662808440278297
1109	22	-1.237640041200958
1109	23	-1.4347277082811587
1109	24	-1.250030116145794
1109	25	-1.1178716594595492
1110	1	-2.2801288170663554
1110	2	-1.9678000498279573
1110	3	-1.3328667033959185
1110	4	-2.1412534661524454
1110	5	-5.385542583580499
1110	6	-3.857657633664136
1110	7	-8.453779911361444
1110	8	-9.683759146120664
1110	9	-11.22418459857333
1110	10	-9.214810067873238
1110	11	-11.661731109818469
1110	12	-6.572529279891249
1110	13	-4.821733465968546
1110	14	-4.203592145429379
1110	15	-9.561295090489729
1110	16	-11.271025946029454
1110	17	-12.020580343966511
1110	18	-8.724242284025891
1110	19	-11.894916114777693
1110	20	-13.043194454584842
1110	21	-13.951048785657145
1110	22	-14.766318800371893
1110	23	-15.382519051773361
1110	24	-15.880624776314402
1110	25	-16.353964216588096
1111	1	-0.6537361241837409
1111	2	0.0819215352424898
1111	3	-2.4042678822651453
1111	4	-3.3864675675376326
1111	5	-1.0772966813361755
1111	6	-0.46037564134242004
1111	7	0.055443486754025884
1111	8	0.3935567330796701
1111	9	-4.118569922857478
1111	10	-4.01123436872572
1111	11	-1.4859725546846647
1111	12	-5.905122411739626
1111	13	-1.299054329092872
1111	14	0.20759908469056754
1111	15	0.25904529249629604
1111	16	0.2154752289972066
1111	17	0.21247383889632587
1111	18	0.3786105939619411
1111	19	0.506555512427834
1111	20	0.5259401802247865
1111	21	0.8459015854930136
1111	22	0.4384931782421262
1111	23	0.25411617559058897
1111	24	0.4561860487685081
1111	25	0.6791321774477801
1112	1	-0.27200907091988924
1112	2	0.3284320806479073
1112	3	-2.4785529036641236
1112	4	-0.4247044109711986
1112	5	-0.49002625496197894
1112	6	-0.29663760034792475
1112	7	0.14828899475250168
1112	8	-3.4731813583903772
1112	9	-0.717268412651741
1112	10	-3.0372993933809376
1112	11	-0.7158722791185141
1112	12	0.3854622090049169
1112	13	0.7524502787936882
1112	14	1.5721578088204247
1112	15	0.9537837665178976
1112	16	0.6610227350305529
1112	17	0.5713168709361547
1112	18	0.5450957573755915
1112	19	0.7169636091617626
1112	20	0.6691459622182201
1112	21	0.5674139241088199
1112	22	0.5088715627132672
1112	23	0.46973881429304143
1112	24	0.6790258374694993
1112	25	0.6644945520606278
1113	1	-1.0082131887010277
1113	2	-0.4173406245843623
1113	3	-2.7123190372211496
1113	4	-1.128590157689068
1113	5	-1.3292203404542597
1113	6	-1.0719919075350397
1113	7	-0.8855708834040144
1113	8	-7.97871264494621
1113	9	-9.54186570219089
1113	10	-3.2954421847531528
1113	11	-8.164586671266111
1113	12	-6.869308275335243
1113	13	-2.579231194841043
1113	14	-0.9172633446104825
1113	15	-6.967860862084347
1113	16	-2.940974610383023
1113	17	-2.075788724749291
1113	18	-1.347089869669098
1113	19	-1.1284009139086066
1113	20	-6.320892541165293
1113	21	-2.5798455650823193
1113	22	-1.9256930778869155
1113	23	-1.7286027856874604
1113	24	-7.3035220388429885
1113	25	-4.27895469804994
1114	1	-3.990389603846161
1114	2	-4.0764280820914855
1114	3	-0.7860278199800419
1114	4	-0.5882894260039393
1114	5	-0.4300933745494362
1114	6	-0.41176363266699667
1114	7	-0.5053028030826496
1114	8	-0.1410044043138292
1114	9	-0.45549244808889355
1114	10	-3.5650836571836066
1114	11	-1.4281571931990271
1114	12	-4.815739739961796
1114	13	-1.4139417729298493
1114	14	-5.860151306988389
1114	15	-2.2159129507876294
1114	16	-1.2808191141423215
1114	17	-1.052692526380497
1114	18	-0.7637470161156656
1114	19	-0.4697966668796276
1114	20	-0.18099555757947172
1114	21	0.03671875148243231
1114	22	-0.0066154851019000205
1114	23	-0.6644642686510096
1114	24	-0.3779780402639048
1114	25	-0.26163474624721417
1115	1	0.7431524373164109
1115	2	0.8260441876153379
1115	3	1.590496405123286
1115	4	0.45991161655593515
1115	5	0.31266746280351043
1115	6	-0.31504949567998963
1115	7	-2.318267966012467
1115	8	-0.19915970864472854
1115	9	0.1512836402367519
1115	10	0.8573796705250203
1115	11	0.09620185648873658
1115	12	0.8251466746274332
1115	13	1.0658063454864433
1115	14	1.8059453914496082
1115	15	0.7704717093705327
1115	16	0.48289400011511674
1115	17	0.049478891908552924
1115	18	0.4546485286760351
1115	19	0.6562420830033204
1115	20	0.7066181223924701
1115	21	0.7597077658612363
1115	22	0.25850200026865977
1115	23	0.1127552105831704
1115	24	0.2864653983842551
1115	25	0.33991725046242394
1116	1	0.5016194962042242
1116	2	0.6860728176618203
1116	3	1.5068118886961983
1116	4	0.8884096899475438
1116	5	0.006201692184991003
1116	6	-0.9063138541699018
1116	7	-4.3114416290191
1116	8	-0.9432898867670012
1116	9	-0.8964671044921921
1116	10	-0.13510365897220566
1116	11	-1.8756550065258466
1116	12	-0.12054300481208813
1116	13	-6.088583125809823
1116	14	-1.3346805451536312
1116	15	-0.775069603836215
1116	16	-0.4565351541807985
1116	17	-0.057046241761806726
1116	18	0.17721956977315045
1116	19	0.1624155920137511
1116	20	0.33056643596847834
1116	21	0.48854628676911815
1116	22	0.22506586481161506
1116	23	0.11942349448593248
1116	24	0.3360221211463852
1116	25	0.3120632289261387
1201	1	1.928910081897584
1201	2	1.996429424578114
1201	3	2.7374078835855684
1201	4	2.1716830796642945
1201	5	1.6572584910843773
1201	6	1.5070326496641862
1201	7	1.851637414053343
1201	8	1.9359525270022644
1201	9	2.333955860342126
1201	10	2.3614105435398507
1201	11	2.011990959710996
1201	12	2.2105638300980637
1201	13	2.3460643588995396
1201	14	3.0956322691043994
1201	15	2.4368306719861828
1201	16	1.9204799435834126
1201	17	1.9139542777743124
1201	18	1.8640084129022298
1201	19	1.9241723822170407
1201	20	2.085349847380879
1201	21	2.0833473579500286
1201	22	1.1668697923262918
1201	23	0.6442441653341575
1201	24	1.2046985527136655
1201	25	1.3100515315592745
1202	1	2.019954067059217
1202	2	2.3710303122304737
1202	3	3.0521021734636804
1202	4	2.646030588938274
1202	5	2.0744087295581473
1202	6	1.8925470243329734
1202	7	2.1858418390665904
1202	8	2.43212038603777
1202	9	2.6027273892239506
1202	10	-1.0301501347855329
1202	11	1.3985933709457576
1202	12	2.3278548343375967
1202	13	2.8291178167691733
1202	14	3.271571259565448
1202	15	2.9584390450651448
1202	16	2.49713435534812
1202	17	2.2285657642608
1202	18	2.365020270347909
1202	19	2.444197554448092
1202	20	2.423030358774625
1202	21	2.6675236298101197
1202	22	2.3697546624905748
1202	23	2.066687196600718
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2211	18	0.5591528606652503
2211	19	0.8397134329114984
2211	20	1.0006605867701417
2211	21	1.0132073160617523
2211	22	0.8344864274252544
2211	23	0.6766752991929827
2211	24	0.8412613767773465
2211	25	0.9397152195454268
2212	1	0.9726294508255897
2212	2	1.1181212688026498
2212	3	1.8404314734490406
2212	4	1.304623872604413
2212	5	0.727407766622715
2212	6	0.6314913922869323
2212	7	0.71890719760518
2212	8	1.1868002663184285
2212	9	1.0618556018243268
2212	10	1.9156988251315745
2212	11	0.9896896673046243
2212	12	1.3281369223307493
2212	13	1.5334876642199298
2212	14	-4.27692658227663
2212	15	-1.0151154255456163
2212	16	-0.20922576983348762
2212	17	0.19114590329515835
2212	18	0.5736508255280519
2212	19	0.9824650871935319
2212	20	1.273647784808798
2212	21	1.1566467884072793
2212	22	1.089781537399066
2212	23	0.9834552248918769
2212	24	1.0216392762530297
2212	25	0.9941971124207356
2213	1	0.8256814713271403
2213	2	0.9229283266657653
2213	3	1.483256647891455
2213	4	0.9174930452165455
2213	5	0.4862268414629902
2213	6	0.12213506451708867
2213	7	0.4592610920089051
2213	8	-4.993912628853252
2213	9	-1.6361141344569923
2213	10	0.3089467764949596
2213	11	0.18916094166957365
2213	12	0.8660378261572177
2213	13	-7.75628749221671
2213	14	-2.038399965269633
2213	15	-0.5280755782158941
2213	16	-0.09465831392761004
2213	17	0.17432393751847997
2213	18	0.3959980776532852
2213	19	0.728284126322631
2213	20	0.9077859656949094
2213	21	0.8323821465753412
2213	22	0.4331216202556689
2213	23	0.47785090670998187
2213	24	0.6249415252894153
2213	25	0.7983992496844792
2214	1	-0.7621658928067774
2214	2	-0.4363644529175623
2214	3	-6.84431919321829
2214	4	-2.4783885520919426
2214	5	-1.3395720622381901
2214	6	-0.7907166378101138
2214	7	-0.5475725913196143
2214	8	-5.945378954982246
2214	9	-1.601186261014984
2214	10	0.1620913324865434
2214	11	-0.1906851310014197
2214	12	0.07600092169708361
2214	13	-5.899067554300331
2214	14	-1.2857788650030244
2214	15	-0.29549995464575574
2214	16	-0.396809990603618
2214	17	-0.2879934992971833
2214	18	-0.19839353294443995
2214	19	0.2534430436238182
2214	20	0.3480998173906862
2214	21	0.31896440451549424
2214	22	0.30503060504452506
2214	23	0.2312748877049131
2214	24	0.38601500512853093
2214	25	0.30301806066171366
2215	1	-0.1603304434392001
2215	2	0.0834231722670502
2215	3	-2.8498813988908793
2215	4	-0.9837129407049865
2215	5	-0.5545108134656758
2215	6	-1.126147488596164
2215	7	-0.27871660827928935
2215	8	0.16624761130804444
2215	9	0.28941823291679825
2215	10	0.7611577106666978
2215	11	0.10240020359617574
2215	12	-0.18990645406206141
2215	13	0.32525144584063526
2215	14	0.48422166063496874
2215	15	0.08540677430559995
2215	16	-0.0413090695424998
2215	17	-0.20199690502179024
2215	18	-0.22244710296505232
2215	19	-0.2248086844110837
2215	20	0.14433949193202267
2215	21	0.21543385795096626
2215	22	0.02975347606558998
2215	23	-0.15259307749238715
2215	24	-0.3138887928790268
2215	25	-0.1701181686639046
2216	1	0.32243582209306965
2216	2	0.04266900694602782
2216	3	1.2517265579708479
2216	4	-0.23702852035566835
2216	5	-0.2494668969256324
2216	6	-0.6075670067054517
2216	7	-1.1624803934138477
2216	8	-6.149682458418397
2216	9	-1.9977476943072219
2216	10	-0.6785003941717989
2216	11	-1.3350713393329698
2216	12	-0.14052284745260835
2216	13	0.4653327007048098
2216	14	1.2699432260650525
2216	15	0.8350272363954865
2216	16	0.11542850596800847
2216	17	0.05622090142777125
2216	18	0.15534466804420788
2216	19	0.30288699434961686
2216	20	0.4284227904109592
2216	21	0.3385990386732196
2216	22	0.14222839413569943
2216	23	-0.1541922986951576
2216	24	0.03079913232255649
2216	25	0.1490493892164544
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	224508.0
3	4.0
4	1628.0
5	9715.0
6	13791.0
7	12312.0
8	10786.0
9	9134.0
10	8210.0
11	7474.0
12	6722.0
13	6614.0
14	6827.0
15	6920.0
16	7202.0
17	7588.0
18	8836.0
19	11118.0
20	15429.0
21	19346.0
22	24098.0
23	28342.0
24	32520.0
25	36824.0
26	40738.0
27	44527.0
28	48502.0
29	55604.0
30	63116.0
31	71218.0
32	87566.0
33	108068.0
34	132581.0
35	163095.0
36	201953.0
37	232588.0
38	300238.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	46.502773648878396	17.025006557663723	12.653959371630089	23.81826042182779
2	15.016569464968041	20.087913860155854	40.663050627463434	24.232466047412668
3	16.892868802761065	26.12926169488363	29.785807564945664	27.192061937409633
4	10.973803878891523	15.92857281666602	37.02213455633721	36.07548874810525
5	12.830772086171402	38.605085957135856	33.73980954990752	14.82433240678523
6	32.230949488357545	36.19619062602273	17.898211460752066	13.674648424867659
7	29.350762996977537	31.720997179552924	21.104451722182077	17.82378810128746
8	26.31426366805564	35.09298507411952	20.34386291712414	18.24888834070069
9	27.043571325903105	16.20269834548612	19.20701624212853	37.54671408648225
10	13.358645030189303	30.226492693313627	33.49977151413209	22.915090762364976
11	34.90880025794848	23.78496653759531	22.318710102237336	18.98752310221888
12	22.852253618040404	25.932037217875887	30.302190078049545	20.913519086034167
13	27.907868810749026	22.400118863917324	26.409348297497743	23.28266402783591
14	21.250803652418142	21.255902353560447	28.480664754087215	29.01262923993419
15	23.59095347435897	29.566828564367327	23.425041914882563	23.417176046391145
16	23.33793497266937	28.83474935309173	24.867255734770765	22.96005993946813
17	22.208244013876786	28.320583815435857	26.275821718769343	23.195350451918014
18	21.99877253190543	27.81516505755699	27.748499167768863	22.437563242768718
19	23.299731538475356	27.0178435387395	27.637601704869063	22.04482321791608
20	23.403461348341718	26.71782985563768	27.746250822230785	22.132457973789812
21	23.992326184733848	26.210225829836887	27.585481902877003	22.211966082552266
22	23.505087761210262	26.2695124722499	27.424965476252904	22.80043429028693
23	23.22676595087646	26.50836933648355	27.253389345786587	23.011475366853407
24	22.892405760902445	26.652258421952457	27.59934721037316	22.85598860677194
25	22.857818437118553	26.38946478112804	27.694083237279365	23.05863354447404
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	8233.0
1	8233.0
2	6115.5
3	3998.0
4	3998.0
5	3998.0
6	6620.5
7	9243.0
8	9243.0
9	9243.0
10	9829.5
11	10416.0
12	10416.0
13	10416.0
14	12345.5
15	14275.0
16	14275.0
17	14275.0
18	21397.5
19	28520.0
20	28520.0
21	28520.0
22	39105.5
23	49691.0
24	49691.0
25	49691.0
26	67578.0
27	85465.0
28	85465.0
29	85465.0
30	105286.5
31	125108.0
32	125108.0
33	125108.0
34	143973.5
35	162839.0
36	162839.0
37	162839.0
38	181729.5
39	200620.0
40	200620.0
41	200620.0
42	217789.0
43	234958.0
44	234958.0
45	234958.0
46	247709.5
47	260461.0
48	260461.0
49	260461.0
50	258186.5
51	255912.0
52	255912.0
53	255912.0
54	238990.5
55	222069.0
56	222069.0
57	222069.0
58	195452.0
59	168835.0
60	168835.0
61	168835.0
62	140366.0
63	111897.0
64	111897.0
65	111897.0
66	87251.5
67	62606.0
68	62606.0
69	62606.0
70	46482.5
71	30359.0
72	30359.0
73	30359.0
74	21187.0
75	12015.0
76	12015.0
77	12015.0
78	8633.5
79	5252.0
80	5252.0
81	5252.0
82	3581.5
83	1911.0
84	1911.0
85	1911.0
86	1311.0
87	711.0
88	711.0
89	711.0
90	503.5
91	296.0
92	296.0
93	296.0
94	166.5
95	37.0
96	37.0
97	37.0
98	26.0
99	15.0
100	15.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.34283080849399394
2	0.3454448813065717
3	0.3585152453694605
4	0.35928978546207613
5	0.3662122375398283
6	0.304781526444251
7	0.3143664600903695
8	0.31141352598727234
9	0.31010648958098347
10	0.4222211679870962
11	0.31059057713886823
12	0.31025171584834893
13	0.3022642711432502
14	0.3095255845115218
15	0.3006667822022305
16	0.3064758328968477
17	0.299504972063307
18	0.30100564349274983
19	0.3015381398064231
20	0.5014178924570445
21	0.3035713075495391
22	0.3044910739095202
23	0.30013428588855723
24	0.30366812506111607
25	0.2970361255180947
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	2065742.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	69.64513911008832
#Duplication Level	Percentage of deduplicated	Percentage of total
1	87.95871875836124	61.258972038712045
2	6.979457617924457	9.721705934266291
3	2.0431249338425466	4.268811607102625
4	0.9013165350410114	2.510892618606162
5	0.5073041479014949	1.766563397586222
6	0.3239556964758009	1.3537163727937622
7	0.20621429129256086	1.0053276102491076
8	0.16509279190723736	0.9198328366761929
9	0.12063353538498851	0.7561385417906359
>10	0.7169303654342037	9.324764880981817
>50	0.05164838779316411	2.453585762843955
>100	0.024260004151621904	2.9597275411202584
>500	7.068076261559633E-4	0.30022763851554135
>1k	4.947653383091576E-4	0.8125196591797642
>5k	1.4136152523118786E-4	0.5872135595756336
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	6145	0.297471804320191	No Hit
NNNNNNNNNNNNNNNNNNNNNNNNN	5784	0.2799962434805508	No Hit
TATCAACGCAGAGTACTTTTTTTTT	3977	0.19252162177077292	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	3310	0.16023298165985878	No Hit
GGTATCAACGCAGAGTACTTTTTTT	2750	0.13312407841831167	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.452262673654309E-4
2	0.0	0.0	0.0	0.0	1.452262673654309E-4
3	0.0	0.0	0.0	0.0	1.452262673654309E-4
4	0.0	0.0	0.0	4.840875578847697E-5	1.452262673654309E-4
5	0.0	0.0	0.0	4.840875578847697E-5	2.4204377894238488E-4
6	0.0	0.0	0.0	4.840875578847697E-5	2.4204377894238488E-4
7	4.840875578847697E-5	0.0	0.0	4.840875578847697E-5	2.4204377894238488E-4
8	9.681751157695394E-5	0.0	0.0	4.840875578847697E-5	2.4204377894238488E-4
9	9.681751157695394E-5	0.0	0.0	4.840875578847697E-5	2.4204377894238488E-4
10	9.681751157695394E-5	0.0	0.0	4.840875578847697E-5	2.904525347308618E-4
11	9.681751157695394E-5	0.0	0.0	4.840875578847697E-5	2.904525347308618E-4
12	9.681751157695394E-5	0.0	0.0	4.840875578847697E-5	3.8727004630781576E-4
13	9.681751157695394E-5	0.0	0.0	4.840875578847697E-5	4.3567880209629276E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGCACG	55	1.9464665E-4	13.830003	18
TAACGCA	125	7.2759576E-12	13.686041	4
TGCCGTA	65	5.410575E-5	13.165099	15
GGTATCA	1780	0.0	12.960013	1
CGTCTCG	105	1.9703293E-8	12.677812	17
ATAACGC	145	7.2759576E-12	12.439554	3
GTTATCA	390	0.0	11.684114	1
TATAACG	155	2.5465852E-11	11.637001	2
CTTTACG	90	7.3910196E-6	11.621326	17
TTTACGG	90	7.3930223E-6	11.621044	18
GTATCAA	4410	0.0	11.107858	1
AACGCAT	310	0.0	11.031763	6
ACTGCGT	90	9.524259E-5	10.554826	8
TCGAGCC	145	1.4713805E-8	10.470072	13
GTCTTAT	300	0.0	10.4426775	1
ACGCATA	315	0.0	10.253261	7
GTCTAGA	250	0.0	10.2528105	1
CATGGGT	1185	0.0	10.185931	4
GTATAAA	365	0.0	10.143573	1
ATGGGTA	600	0.0	10.137808	5
>>END_MODULE