FastQCFastQC Report
Thu 26 May 2016
SRR1364301_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364301_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2065742
Sequences flagged as poor quality0
Sequence length25
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT130360.6310565404585858No Hit
TATCAACGCAGAGTACTTTTTTTTT101750.49255909014775323No Hit
GGTATCAACGCAGAGTACTTTTTTT91270.4418267140814293No Hit
GTACTTTTTTTTTTTTTTTTTTTTT76130.36853585781767517No Hit
ACGCAGAGTACTTTTTTTTTTTTTT45500.22025983883757022No Hit
GAGTACTTTTTTTTTTTTTTTTTTT43810.21207875910931764No Hit
GTGGTATCAACGCAGAGTACATGGG24260.11743964154284514No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCTCGA1400.015.60750210
CGCGCCA1006.6029315E-1014.25032710
TATAACG1950.014.1275022
TAACGCA2000.013.776654
ACCGCGC852.6973248E-713.4130478
TATCACG1550.012.8703282
CTGCTCG1950.012.6678779
ATAACGC2100.012.6675723
GGTATCA27950.012.6458281
ACGAAAC853.9536953E-612.29172117
ATCACGC1551.8189894E-1212.2589393
AGGACCG1501.4551915E-1112.0365245
GAATGCG802.8717339E-511.8732611
TTGCGCG1459.822543E-1111.7905218
GACCGTC658.0189476E-411.6934267
TGCGTAG658.041786E-411.68946314
TAGACAG2800.011.53877455
GTGGTAT9250.011.504361
CTAGACA1900.011.5013784
GCACCGC1001.9281815E-611.4010916
Produced by FastQC (version 0.11.5)