##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1364301_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2065742 Sequences flagged as poor quality 0 Sequence length 25 %GC 47 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.629839544338065 31.0 30.0 34.0 26.0 34.0 2 29.642950571755815 31.0 30.0 34.0 26.0 34.0 3 29.687436766062753 31.0 30.0 34.0 26.0 34.0 4 33.048805223498384 35.0 33.0 37.0 27.0 37.0 5 32.61394598163759 35.0 33.0 37.0 25.0 37.0 6 32.51517566085213 35.0 33.0 37.0 25.0 37.0 7 32.2394597195584 35.0 32.0 37.0 22.0 37.0 8 32.12720562393561 35.0 32.0 37.0 22.0 37.0 9 32.8562458428981 37.0 32.0 39.0 17.0 39.0 10 32.995664511831585 37.0 32.0 39.0 17.0 39.0 11 32.95411430856322 37.0 32.0 39.0 17.0 39.0 12 32.81565703751969 37.0 32.0 39.0 17.0 39.0 13 32.932373452251056 37.0 32.0 39.0 17.0 39.0 14 33.86264257588799 38.0 33.0 41.0 17.0 41.0 15 33.893779571698694 38.0 33.0 41.0 17.0 41.0 16 33.826537873558266 38.0 33.0 41.0 17.0 41.0 17 33.77565930304946 38.0 33.0 41.0 17.0 41.0 18 33.730484252147654 38.0 33.0 41.0 17.0 41.0 19 33.62475468863004 38.0 32.0 41.0 11.0 41.0 20 33.45263348472365 38.0 32.0 41.0 10.0 41.0 21 33.36925908462916 38.0 32.0 41.0 10.0 41.0 22 33.35762888105097 38.0 32.0 41.0 8.0 41.0 23 33.05103977166558 38.0 32.0 41.0 2.0 41.0 24 32.9630583102827 38.0 32.0 41.0 2.0 41.0 25 32.82878936478999 38.0 32.0 41.0 2.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.14792812340004602 1101 2 0.3673532150040515 1101 3 -1.531905848053082 1101 4 -0.4819411240731313 1101 5 0.32375316552376887 1101 6 0.5545666624730359 1101 7 0.7050556155963577 1101 8 0.8222095589519824 1101 9 1.513623378880169 1101 10 1.1919180834215837 1101 11 1.2592103188032624 1101 12 1.216495418943147 1101 13 1.1191597365332413 1101 14 1.2329631420576987 1101 15 1.1962381994635578 1101 16 1.2669060649473778 1101 17 1.0665147640424593 1101 18 1.1855166314088947 1101 19 1.149947930396067 1101 20 1.3610940731242778 1101 21 1.272436993713761 1101 22 1.458855624124027 1101 23 1.7114048715109504 1101 24 1.6196031486628613 1101 25 1.7466707129369325 1102 1 0.37400900917158353 1102 2 0.41302667636864854 1102 3 0.596496761689238 1102 4 0.4531826069651643 1102 5 0.4931406326408023 1102 6 0.6082807722032229 1102 7 0.8124788837356434 1102 8 0.9268074052263344 1102 9 1.4912707388605213 1102 10 1.1186595249392965 1102 11 1.0043097735142723 1102 12 1.2001183302902376 1102 13 0.9037076536857569 1102 14 0.9441475327208906 1102 15 0.9860250104315256 1102 16 1.0357301707511724 1102 17 1.0337207781151037 1102 18 0.9769408442602483 1102 19 1.00194135702219 1102 20 1.164077145052616 1102 21 1.398750542063162 1102 22 1.393413518188531 1102 23 1.6714640497005746 1102 24 1.7298286664843943 1102 25 1.5257350419858895 1103 1 -2.73698114116538 1103 2 -2.7120656151311096 1103 3 -2.5128470653839834 1103 4 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0.25580976286478574 1107 15 0.16122018830987628 1107 16 0.036322160157880035 1107 17 -0.0725270497361663 1107 18 -0.0028626867070684625 1107 19 0.12832381440924223 1107 20 -0.05613689532847843 1107 21 -0.07864367550702411 1107 22 -0.017719144521187502 1107 23 0.3649173147677658 1107 24 0.564138811462584 1107 25 0.33147427152594844 1108 1 -0.10160438509896608 1108 2 0.043130019374871154 1108 3 0.10598493275836063 1108 4 -0.12288295019922657 1108 5 0.08507817000656104 1108 6 0.1746772136921777 1108 7 -0.3544447997483431 1108 8 0.15802581192068743 1108 9 0.43554863879080585 1108 10 0.21672623808350266 1108 11 0.32847590886719047 1108 12 0.2906509152160268 1108 13 -0.12557160680496082 1108 14 -0.011298880067187156 1108 15 -0.31209979963622203 1108 16 -0.8628699416688619 1108 17 -0.9081025516231733 1108 18 -0.9561287191090955 1108 19 -0.747183812551313 1108 20 -0.0302210143176751 1108 21 -0.4321852255809233 1108 22 -0.2332650856579832 1108 23 -0.6907960894539684 1108 24 -0.3486641503523913 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0.3803101536987903 2209 2 0.38980075829980976 2209 3 0.38259155640799847 2209 4 0.3624301388441964 2209 5 0.3608061431539724 2209 6 0.5521818234733473 2209 7 0.4899561870145348 2209 8 0.6174502407598723 2209 9 1.4507027957991525 2209 10 0.7586604316054206 2209 11 0.6302625362092158 2209 12 1.0246004435254363 2209 13 0.5533650171561746 2209 14 0.5587392137816565 2209 15 0.42329648199689984 2209 16 0.31249130030913363 2209 17 0.19454095939281757 2209 18 0.6031564476141753 2209 19 0.7047259322259833 2209 20 0.9780993842773995 2209 21 0.9909831888898779 2209 22 0.9848512593686607 2209 23 1.248064384796976 2209 24 1.1747460164658463 2209 25 1.2589699064439301 2210 1 7.311019087943293E-4 2210 2 -0.04992138462226592 2210 3 -0.063027150416616 2210 4 0.02694037317036191 2210 5 0.1625145784034956 2210 6 0.06112909499012886 2210 7 0.2774497831955145 2210 8 0.12109662980337532 2210 9 0.9290901834786922 2210 10 0.4097377528801758 2210 11 0.3326223313366228 2210 12 0.5804750603373847 2210 13 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0.9107020339222558 2211 25 0.9235193668487369 2212 1 -0.057103676106983414 2212 2 -0.06725449415686313 2212 3 -0.035097136456570865 2212 4 -0.01070615041225409 2212 5 -0.13690931094821934 2212 6 0.06715489309868872 2212 7 0.13016754338763548 2212 8 -0.03297545494858767 2212 9 0.4352763022422508 2212 10 -0.003831705230879834 2212 11 0.12890585216984363 2212 12 -0.056877479307757994 2212 13 -0.234436170630012 2212 14 -0.2664697900490438 2212 15 -0.1488992344966249 2212 16 0.009689024237175659 2212 17 -0.12826510261520951 2212 18 -0.24128360799944915 2212 19 0.0025379823684517078 2212 20 0.15183404277946977 2212 21 0.25629000518074463 2212 22 -0.073877048806537 2212 23 0.3216369450977439 2212 24 0.5281517017253989 2212 25 0.48602100052136166 2213 1 -0.10575898350510471 2213 2 -0.05692912530548355 2213 3 -0.1237791638233432 2213 4 -0.08154827664892395 2213 5 -0.07480207129186311 2213 6 0.19466414546355537 2213 7 0.08006192842285031 2213 8 0.038468588143786064 2213 9 0.6595513236772987 2213 10 0.0757246151297295 2213 11 0.2792604107346648 2213 12 0.17243965961972663 2213 13 -0.13211314202869318 2213 14 -0.0176461728808448 2213 15 0.031879576161060186 2213 16 -0.16791883435725907 2213 17 -0.032654606733842684 2213 18 -0.21000262146323223 2213 19 -0.024892204788145023 2213 20 0.11221599882401989 2213 21 -0.21591234968754236 2213 22 0.07724096127336111 2213 23 -0.09917953250550937 2213 24 -0.04478553571819788 2213 25 0.21250310605222467 2214 1 -0.8518987474099582 2214 2 -0.7117151898023657 2214 3 -0.6845160536184522 2214 4 -0.544236706683229 2214 5 -0.8520019552483404 2214 6 -0.5122871604376158 2214 7 -0.44232205703374206 2214 8 -0.4953494133509402 2214 9 -10.732286892706238 2214 10 -3.7038233526891915 2214 11 -1.5352024957389254 2214 12 -0.9171685994267698 2214 13 -0.7441101740829481 2214 14 -0.7556619664241708 2214 15 -0.8689878777134084 2214 16 -0.9264450010120768 2214 17 -0.8304543154155581 2214 18 -1.1470338929074728 2214 19 -1.3222924469206419 2214 20 -1.0407100600521062 2214 21 -1.6494151959287002 2214 22 -1.4392325822989847 2214 23 -1.678929383797449 2214 24 -1.952624168030745 2214 25 -9.892031309637236 2215 1 -0.8784608735542037 2215 2 -0.6025942783870448 2215 3 -0.5673203698572884 2215 4 -0.765715213721144 2215 5 -0.6898264215124499 2215 6 -0.8308132032402469 2215 7 -0.9691947021158 2215 8 -0.9865204870143245 2215 9 -0.19236733238535209 2215 10 -0.2982658231467994 2215 11 -0.29343094893307864 2215 12 -0.29386757917707484 2215 13 -0.2798581654802348 2215 14 -0.4986688478446766 2215 15 -1.0202346637272015 2215 16 -0.6824499030983517 2215 17 -0.7594443771029304 2215 18 -0.512882629311278 2215 19 -0.7327850857701392 2215 20 -0.6575692842991714 2215 21 -0.46049074683315183 2215 22 -0.44104108418633103 2215 23 -0.14402369315179442 2215 24 -0.048786737622315 2215 25 0.05753688216465491 2216 1 -0.4940812906422103 2216 2 -0.477893739423358 2216 3 -0.5227867084514273 2216 4 -0.3014003805722325 2216 5 -1.2212036510908604 2216 6 -1.7776620128081788 2216 7 -1.084533526560822 2216 8 -1.987910250939759 2216 9 -0.4494637317055634 2216 10 -1.0798660767456276 2216 11 -0.46756803789385515 2216 12 -1.7303123512822651 2216 13 -1.4651735889479198 2216 14 -1.5529662626190586 2216 15 -1.2028979543705205 2216 16 -2.1703834432638516 2216 17 -2.604539304596962 2216 18 -2.008573037557742 2216 19 -3.4479688522686196 2216 20 -2.1507485214908435 2216 21 -2.349654757506542 2216 22 -2.0067858520560513 2216 23 -1.5793248880840487 2216 24 -1.7669555184949282 2216 25 -1.8464300996916485 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 73999.0 3 1.0 4 1101.0 5 10672.0 6 13613.0 7 11060.0 8 8744.0 9 7270.0 10 6434.0 11 5634.0 12 5174.0 13 4977.0 14 4826.0 15 4851.0 16 5127.0 17 5587.0 18 6132.0 19 7436.0 20 10077.0 21 13212.0 22 16296.0 23 19477.0 24 22577.0 25 25626.0 26 29021.0 27 31596.0 28 35271.0 29 41455.0 30 50307.0 31 62035.0 32 76607.0 33 94185.0 34 116167.0 35 145187.0 36 188124.0 37 274323.0 38 631561.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.39765228957936 18.100671085692593 12.496313846717037 25.005362778011015 2 16.97751783150331 20.454116373613925 38.02401673794104 24.544349056941726 3 19.61388189516498 25.058030024093046 28.579817121313855 26.748270959428115 4 12.663155927736483 16.301021173841608 35.65481671251153 35.38100618591037 5 13.212573358391852 38.24902556036635 33.985510158917556 14.552890922324243 6 32.67174397285734 36.39529012152323 17.304170210953018 13.628795694666406 7 29.252993801053673 31.506944824390626 20.913007038513065 18.32705433604264 8 27.06648776611739 35.341009400026245 19.14563528014383 18.446867553712533 9 26.731451637896303 15.276007608392476 18.768546425140237 39.22399432857099 10 15.105588173194823 30.10720560347583 32.042359239982105 22.744846983347248 11 35.693344761960105 22.739432152213812 22.003447958585756 19.563775127240326 12 23.34223143984725 25.61003830349224 29.821195502728493 21.226534753932015 13 29.18108274664507 21.57021548721469 25.5971488475285 23.65155291861174 14 22.31270768301279 20.699122018853163 27.453370564587964 29.534799733546084 15 24.27330359239258 29.002355230766625 23.317752824219422 23.406588352621373 16 23.67654861772748 27.975574946358993 25.148045298569315 23.19983113734421 17 22.504789288261172 27.47616006801104 26.86658788204771 23.152462761680077 18 22.220366316965702 26.74764546141597 28.418734831000457 22.613253390617867 19 23.587842659919257 26.064474146721246 28.162580747618122 22.18510244574138 20 23.359452087160204 25.717257066055723 28.556993722396896 22.366297124387177 21 23.88943731490622 25.102311442178603 28.394432940677518 22.61381830223766 22 23.567875007504117 25.20541923339847 28.37937151300488 22.847334246092533 23 22.944830778108923 25.326882819867812 28.574845127645034 23.153441274378235 24 22.920051432300493 25.70487038324422 28.47589207737308 22.899186107082205 25 22.968472554994687 25.29397055060597 28.566706987058705 23.17084990734064 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 1008.0 1 1008.0 2 1431.5 3 1855.0 4 1855.0 5 1855.0 6 5682.0 7 9509.0 8 9509.0 9 9509.0 10 9235.5 11 8962.0 12 8962.0 13 8962.0 14 9389.5 15 9817.0 16 9817.0 17 9817.0 18 16769.0 19 23721.0 20 23721.0 21 23721.0 22 34904.0 23 46087.0 24 46087.0 25 46087.0 26 65415.0 27 84743.0 28 84743.0 29 84743.0 30 103032.5 31 121322.0 32 121322.0 33 121322.0 34 139769.5 35 158217.0 36 158217.0 37 158217.0 38 175096.5 39 191976.0 40 191976.0 41 191976.0 42 208979.5 43 225983.0 44 225983.0 45 225983.0 46 242050.0 47 258117.0 48 258117.0 49 258117.0 50 261109.5 51 264102.0 52 264102.0 53 264102.0 54 248240.0 55 232378.0 56 232378.0 57 232378.0 58 206695.0 59 181012.0 60 181012.0 61 181012.0 62 151512.0 63 122012.0 64 122012.0 65 122012.0 66 95288.0 67 68564.0 68 68564.0 69 68564.0 70 51283.0 71 34002.0 72 34002.0 73 34002.0 74 23522.5 75 13043.0 76 13043.0 77 13043.0 78 9599.5 79 6156.0 80 6156.0 81 6156.0 82 4109.0 83 2062.0 84 2062.0 85 2062.0 86 1362.5 87 663.0 88 663.0 89 663.0 90 535.5 91 408.0 92 408.0 93 408.0 94 214.5 95 21.0 96 21.0 97 21.0 98 11.5 99 2.0 100 2.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.028125487113105124 2 7.745400926156316E-4 3 4.840875578847698E-5 4 0.003872700463078158 5 0.034321807854030176 6 0.010214247471368641 7 0.018927823513294497 8 0.02589868434683518 9 0.03165932628566394 10 0.035725661771896004 11 0.036403384352934685 12 0.006486773275655914 13 0.0024688465452123256 14 0.004550423044116836 15 0.0061479119851365754 16 0.007648583414579361 17 0.00943970737875301 18 0.012731502772369443 19 0.017233517060697805 20 0.014716261759697001 21 0.011376057610292088 22 0.010165838715580165 23 0.008229488484041085 24 0.005518598159886375 25 0.10988787563984273 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 2065742.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 52.492640823926116 #Duplication Level Percentage of deduplicated Percentage of total 1 77.78807921597657 40.83301702667371 2 12.264376580918638 12.875790295830663 3 3.9975655815817612 6.295283227321822 4 1.7931761293541757 3.765142019689073 5 0.9743291311940455 2.557255456402851 6 0.6124783845480107 1.929036471149834 7 0.4052923111521067 1.4892404602604512 8 0.30775348370464034 1.2923834465935748 9 0.2380305126714689 1.1245365186118574 >10 1.4266947461329678 14.055317926337707 >50 0.12911163944219017 4.63372536839906 >100 0.0598601155020682 5.527726374476106 >500 0.0020442197735617462 0.6910538235415133 >1k 8.36271725547986E-4 0.9818544231995093 >5k 1.8583816123288579E-4 0.8165048705593536 >10k+ 1.8583816123288579E-4 1.1321322909529963 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 13036 0.6310565404585858 No Hit TATCAACGCAGAGTACTTTTTTTTT 10175 0.49255909014775323 No Hit GGTATCAACGCAGAGTACTTTTTTT 9127 0.4418267140814293 No Hit GTACTTTTTTTTTTTTTTTTTTTTT 7613 0.36853585781767517 No Hit ACGCAGAGTACTTTTTTTTTTTTTT 4550 0.22025983883757022 No Hit GAGTACTTTTTTTTTTTTTTTTTTT 4381 0.21207875910931764 No Hit GTGGTATCAACGCAGAGTACATGGG 2426 0.11743964154284514 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 4.840875578847697E-5 0.0 0.0 0.0 0.0 8 4.840875578847697E-5 0.0 0.0 0.0 0.0 9 4.840875578847697E-5 0.0 0.0 0.0 0.0 10 4.840875578847697E-5 0.0 0.0 0.0 0.0 11 4.840875578847697E-5 0.0 0.0 4.840875578847697E-5 0.0 12 4.840875578847697E-5 0.0 0.0 4.840875578847697E-5 1.452262673654309E-4 13 4.840875578847697E-5 0.0 0.0 4.840875578847697E-5 1.452262673654309E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TGCTCGA 140 0.0 15.607502 10 CGCGCCA 100 6.6029315E-10 14.250327 10 TATAACG 195 0.0 14.127502 2 TAACGCA 200 0.0 13.77665 4 ACCGCGC 85 2.6973248E-7 13.413047 8 TATCACG 155 0.0 12.870328 2 CTGCTCG 195 0.0 12.667877 9 ATAACGC 210 0.0 12.667572 3 GGTATCA 2795 0.0 12.645828 1 ACGAAAC 85 3.9536953E-6 12.291721 17 ATCACGC 155 1.8189894E-12 12.258939 3 AGGACCG 150 1.4551915E-11 12.036524 5 GAATGCG 80 2.8717339E-5 11.87326 11 TTGCGCG 145 9.822543E-11 11.79052 18 GACCGTC 65 8.0189476E-4 11.693426 7 TGCGTAG 65 8.041786E-4 11.689463 14 TAGACAG 280 0.0 11.5387745 5 GTGGTAT 925 0.0 11.50436 1 CTAGACA 190 0.0 11.501378 4 GCACCGC 100 1.9281815E-6 11.401091 6 >>END_MODULE